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The map label for this gene is citG

Identifier: 16271996

GI number: 16271996

Start: 22689

End: 23939

Strand: Reverse

Name: citG

Synonym: HI0021

Alternate gene names: 16271996

Gene position: 23939-22689 (Counterclockwise)

Preceding gene: 16271997

Following gene: 16271995

Centisome position: 1.31

GC content: 37.65

Gene sequence:

>1251_bases
GTGGGTGGAGTAAAAAAAAATGCTTTGTTGGATTATGTTTTTACAAAAGCTCTAGAAAATCTGACCGCACTTTTTACTCA
GTTAAACATCACAGCAGTAAAAGAAATAATCCGTCCGCTAGAAACGGGGCATGAAGCCTATTTTGTATTACCGATAGATG
CTCGTACGCTAAAAGTTCTGATGATTGAATTGGAAGAAAGTATTCCTCTTGCTCGTTTGTGGGATTTAGATGTGTTTAAT
GCTAAAGGCAATCTACTGAGTCGTACAGATTTTGATCTTTCTCCAAGAACTTGTTTAGTGTGCGGAGAAAATGCAAAAAT
CTGTGCGAGAACCCACAAGCACGAGATTGATGAGATTGTAGATAAAATTCAATCTCTCGCACAGAATCATGATTTCGCAG
AACATATTGGTGAACAAGTTTATCTGGCATTAATACAAGAGGCTCGCTTATCGCCAAAACCTGGTTTAGTGGATGCCATC
AATAACGGTTCGCACAAAGATATGAACCTACATACTTTTGAGCAAAGTGCGATTAGTTTAAAACCTTTTTTTACGCAGTT
TGTTCTCAAAGGCATGATGACCGCGCATTTGTCTGAAAATCAAATTCTCTCTGAAATTCGTCCTTTAGGTTTATTAGCAG
AAAAAGCAATGTTTAAAGTTACTGATGGCGTAAACACCCACAAAGGAGCTATTTTTTCTTTTGGTTTAGTGTGTACGGCA
ATTGGACGATTATTGGCACAAAAAAGTTTGGTTCAAAGTGCGGTAGATTTTGATGTTAAATTAATCTGCTCACTTGTTGC
ACAATTTACTCAAGGTTTAACGGATGAACTGAAAAATTATCCTGAACACTTGCCTAGTACGGCTGGAGTGCGTTTATTCC
AAAAATATGGCTTAACAGGTGTCCGTGGCGAAGCAGAAAATGGTTTTAATCTCATTCAAACGTTGTTGCCACAATTTGAT
GAGTACCATCAGTTAGAATGGGAACATCGTTTACTGATTTTGTTGTTGAATTTAATGGCAATTAACTCCGACACTAACGT
GGTTCATCGAGGTGGTTTAGCGGGATTGTATTTCATACAGCAAACAGCACAGGATCTGCTTACAGATCAACACCTTGTGA
CAGATAAAACAGCACTGACACAAGCATTAATGAAATTTGATACCGCCTGCATTGAACGTAATTTAAGTTCGGGCGGTAGT
GCTGATTTATTAGCACTAACAATTTTCTTTTTATCATTTAGAGGTAATTAA

Upstream 100 bases:

>100_bases
ACTTGAAGCATTGCTTAATGCTCGAGAGGAACGTGCAATTTTACAACAACAGCTTATAACACAATATGGACAAACCTTAC
TTTGCATCACTTTAACTGCT

Downstream 100 bases:

>100_bases
TATGGCATTATCCAAAAATACAAAACTGGTGATTTTAATGGCTATCCCATTGATCACTTTCTTACTACCTGCTCCTGATG
GGCTTTCTTTGATTGCTTGG

Product: CitG protein

Products: NA

Alternate protein names: Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase; Apo-ACP nucleodityltransferase; Holo-ACP synthase; Holo-citrate lyase synthase; 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase; 2-(5''-triphosphoribosyl)-3'-dephospho-CoA synthase

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MGGVKKNALLDYVFTKALENLTALFTQLNITAVKEIIRPLETGHEAYFVLPIDARTLKVLMIELEESIPLARLWDLDVFN
AKGNLLSRTDFDLSPRTCLVCGENAKICARTHKHEIDEIVDKIQSLAQNHDFAEHIGEQVYLALIQEARLSPKPGLVDAI
NNGSHKDMNLHTFEQSAISLKPFFTQFVLKGMMTAHLSENQILSEIRPLGLLAEKAMFKVTDGVNTHKGAIFSFGLVCTA
IGRLLAQKSLVQSAVDFDVKLICSLVAQFTQGLTDELKNYPEHLPSTAGVRLFQKYGLTGVRGEAENGFNLIQTLLPQFD
EYHQLEWEHRLLILLLNLMAINSDTNVVHRGGLAGLYFIQQTAQDLLTDQHLVTDKTALTQALMKFDTACIERNLSSGGS
ADLLALTIFFLSFRGN

Sequences:

>Translated_416_residues
MGGVKKNALLDYVFTKALENLTALFTQLNITAVKEIIRPLETGHEAYFVLPIDARTLKVLMIELEESIPLARLWDLDVFN
AKGNLLSRTDFDLSPRTCLVCGENAKICARTHKHEIDEIVDKIQSLAQNHDFAEHIGEQVYLALIQEARLSPKPGLVDAI
NNGSHKDMNLHTFEQSAISLKPFFTQFVLKGMMTAHLSENQILSEIRPLGLLAEKAMFKVTDGVNTHKGAIFSFGLVCTA
IGRLLAQKSLVQSAVDFDVKLICSLVAQFTQGLTDELKNYPEHLPSTAGVRLFQKYGLTGVRGEAENGFNLIQTLLPQFD
EYHQLEWEHRLLILLLNLMAINSDTNVVHRGGLAGLYFIQQTAQDLLTDQHLVTDKTALTQALMKFDTACIERNLSSGGS
ADLLALTIFFLSFRGN
>Mature_415_residues
GGVKKNALLDYVFTKALENLTALFTQLNITAVKEIIRPLETGHEAYFVLPIDARTLKVLMIELEESIPLARLWDLDVFNA
KGNLLSRTDFDLSPRTCLVCGENAKICARTHKHEIDEIVDKIQSLAQNHDFAEHIGEQVYLALIQEARLSPKPGLVDAIN
NGSHKDMNLHTFEQSAISLKPFFTQFVLKGMMTAHLSENQILSEIRPLGLLAEKAMFKVTDGVNTHKGAIFSFGLVCTAI
GRLLAQKSLVQSAVDFDVKLICSLVAQFTQGLTDELKNYPEHLPSTAGVRLFQKYGLTGVRGEAENGFNLIQTLLPQFDE
YHQLEWEHRLLILLLNLMAINSDTNVVHRGGLAGLYFIQQTAQDLLTDQHLVTDKTALTQALMKFDTACIERNLSSGGSA
DLLALTIFFLSFRGN

Specific function: Bifunctional enzyme that catalyzes formation of 2-(5''- triphosphoribosyl)-3'-dephosphocoenzyme-A, and then the transfer of this prosthetic group precursor to the apo-acyl carrier protein (gamma chain) of the citrate lyase to yield the holo-acyl carrier p

COG id: COG1767

COG function: function code H; Triphosphoribosyl-dephospho-CoA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the CitG/MdcB family

Homologues:

Organism=Escherichia coli, GI1786830, Length=274, Percent_Identity=47.8102189781022, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1786831, Length=130, Percent_Identity=33.0769230769231, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITXG_HAEIN (P44458)

Other databases:

- EMBL:   L42023
- PIR:   E64140
- RefSeq:   NP_438194.1
- GeneID:   950919
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0021
- NMPDR:   fig|71421.1.peg.20
- TIGR:   HI_0021
- HOGENOM:   HBG305005
- OMA:   KGAIFAF
- ProtClustDB:   CLSK892351
- BioCyc:   HINF71421:HI_0021-MONOMER
- BRENDA:   2.7.7.61
- BRENDA:   2.7.8.25
- GO:   GO:0016310
- HAMAP:   MF_00397
- HAMAP:   MF_00398
- InterPro:   IPR002736
- InterPro:   IPR005551
- InterPro:   IPR017551
- TIGRFAMs:   TIGR03125
- TIGRFAMs:   TIGR03124

Pfam domain/function: PF01874 CitG; PF03802 CitX

EC number: =2.7.7.61; =2.7.8.25

Molecular weight: Translated: 46294; Mature: 46163

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGVKKNALLDYVFTKALENLTALFTQLNITAVKEIIRPLETGHEAYFVLPIDARTLKVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHH
MIELEESIPLARLWDLDVFNAKGNLLSRTDFDLSPRTCLVCGENAKICARTHKHEIDEIV
HHHHHHCCCHHHEECCEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
DKIQSLAQNHDFAEHIGEQVYLALIQEARLSPKPGLVDAINNGSHKDMNLHTFEQSAISL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEHHHHHHHHH
KPFFTQFVLKGMMTAHLSENQILSEIRPLGLLAEKAMFKVTDGVNTHKGAIFSFGLVCTA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IGRLLAQKSLVQSAVDFDVKLICSLVAQFTQGLTDELKNYPEHLPSTAGVRLFQKYGLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
VRGEAENGFNLIQTLLPQFDEYHQLEWEHRLLILLLNLMAINSDTNVVHRGGLAGLYFIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
QTAQDLLTDQHLVTDKTALTQALMKFDTACIERNLSSGGSADLLALTIFFLSFRGN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GGVKKNALLDYVFTKALENLTALFTQLNITAVKEIIRPLETGHEAYFVLPIDARTLKVL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHH
MIELEESIPLARLWDLDVFNAKGNLLSRTDFDLSPRTCLVCGENAKICARTHKHEIDEIV
HHHHHHCCCHHHEECCEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
DKIQSLAQNHDFAEHIGEQVYLALIQEARLSPKPGLVDAINNGSHKDMNLHTFEQSAISL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEHHHHHHHHH
KPFFTQFVLKGMMTAHLSENQILSEIRPLGLLAEKAMFKVTDGVNTHKGAIFSFGLVCTA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
IGRLLAQKSLVQSAVDFDVKLICSLVAQFTQGLTDELKNYPEHLPSTAGVRLFQKYGLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
VRGEAENGFNLIQTLLPQFDEYHQLEWEHRLLILLLNLMAINSDTNVVHRGGLAGLYFIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHH
QTAQDLLTDQHLVTDKTALTQALMKFDTACIERNLSSGGSADLLALTIFFLSFRGN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800