Click here to switch to the map view.

The map label for this gene is citE

Identifier: 30995348

GI number: 30995348

Start: 25784

End: 26641

Strand: Reverse

Name: citE

Synonym: HI0023

Alternate gene names: 30995348

Gene position: 26641-25784 (Counterclockwise)

Preceding gene: 16271999

Following gene: 16271997

Centisome position: 1.46

GC content: 42.42

Gene sequence:

>858_bases
ATGTTATTTGTGCCGGGCTCAAATGCCGCCATGTTAAGTAATAGCTTTATTTATAAACCAGATTCCATTATGTTTGACTT
AGAAGATGCGGTTGCTCTCAAAGAAAAAGATTCTGCTCGTTTATTAGTCGCTCATGCACTTCAACATCCGCTTTATAAAG
AAATTGAGACCGTAGTTCGTGTTAATCCGCTAGATTCTGAATTTGGTTTATTGGATTTGAATTCGGTAGTGCGTGCGGGT
GTGGATGTGGTACGTATGCCAAAAACTGAAAGCGCGCAAGATGTGTTGGATATGGATCACGCCATTACTGAGATTGAGAA
AGCTTGCGGTCGAGAAGCAGGTTCGACTAAAATGCTAGCCGCAATCGAATCGCCATTAGGTATCACTCAAGCCAACCAAA
TCGCTTTCGCTTCTAAACGTTTAATTGGTATCGCATTGGGTGCAGAGGATTACGTACGTAACTTAAAAACCGAGCGTTCA
CCAGAGGGGATTGAACTGCTTTTTGCTCGTTGTTCTATTTTACAAGCGGCACGTGCGGCTGGCATTCAGGCTTTTGATAC
CGTATATTCTAACGCGAACAATGAAGAAGGCTTCTTAAAAGAGGCTGCGTTAATTAAACAGCTTGGTTTTGACGGTAAAT
CCTTAATCAACCCACGCCAAATTGAATTGTTACACAACTTATTTGCACCGACACAAAAAGACGTGGAGCAGGCAAAACGC
ATTATTGAAGCGGCAGTAGAAGCTGAACGACAAGGGGCTGGTGTTGTTTCGTTAAACGGTAAAATGATTGACGCACCGAT
TATTGATCGCGCAAAACTTGTATTAGAACGTGCGAAATCAGGTATTCGTGAAGAGTAA

Upstream 100 bases:

>100_bases
CGTTAGATTGCGTGTTACAAGCTCGAGTAAAAGCTGCTGCAATGCGTGCAACTGATGAAGCGATTAACTGGGAGGCTGTG
CTATGAAATTAAGAAGAAGT

Downstream 100 bases:

>100_bases
AGGGAGATAAAAAGATGACAACAAGAGAACAACGTATTGAAAAATATCACGCTGATCGCTCAGTCTATCAAGCGGTGCCT
AAATCTGAATCCCTTTCTCG

Product: citrate lyase subunit beta

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MLFVPGSNAAMLSNSFIYKPDSIMFDLEDAVALKEKDSARLLVAHALQHPLYKEIETVVRVNPLDSEFGLLDLNSVVRAG
VDVVRMPKTESAQDVLDMDHAITEIEKACGREAGSTKMLAAIESPLGITQANQIAFASKRLIGIALGAEDYVRNLKTERS
PEGIELLFARCSILQAARAAGIQAFDTVYSNANNEEGFLKEAALIKQLGFDGKSLINPRQIELLHNLFAPTQKDVEQAKR
IIEAAVEAERQGAGVVSLNGKMIDAPIIDRAKLVLERAKSGIREE

Sequences:

>Translated_285_residues
MLFVPGSNAAMLSNSFIYKPDSIMFDLEDAVALKEKDSARLLVAHALQHPLYKEIETVVRVNPLDSEFGLLDLNSVVRAG
VDVVRMPKTESAQDVLDMDHAITEIEKACGREAGSTKMLAAIESPLGITQANQIAFASKRLIGIALGAEDYVRNLKTERS
PEGIELLFARCSILQAARAAGIQAFDTVYSNANNEEGFLKEAALIKQLGFDGKSLINPRQIELLHNLFAPTQKDVEQAKR
IIEAAVEAERQGAGVVSLNGKMIDAPIIDRAKLVLERAKSGIREE
>Mature_285_residues
MLFVPGSNAAMLSNSFIYKPDSIMFDLEDAVALKEKDSARLLVAHALQHPLYKEIETVVRVNPLDSEFGLLDLNSVVRAG
VDVVRMPKTESAQDVLDMDHAITEIEKACGREAGSTKMLAAIESPLGITQANQIAFASKRLIGIALGAEDYVRNLKTERS
PEGIELLFARCSILQAARAAGIQAFDTVYSNANNEEGFLKEAALIKQLGFDGKSLINPRQIELLHNLFAPTQKDVEQAKR
IIEAAVEAERQGAGVVSLNGKMIDAPIIDRAKLVLERAKSGIREE

Specific function: Represents a citryl-ACP lyase

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily

Homologues:

Organism=Homo sapiens, GI45545437, Length=291, Percent_Identity=26.4604810996564, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI87081764, Length=286, Percent_Identity=76.5734265734266, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17557458, Length=287, Percent_Identity=29.2682926829268, Blast_Score=110, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITE_HAEIN (P44460)

Other databases:

- EMBL:   L42023
- PIR:   D64043
- RefSeq:   NP_438196.2
- ProteinModelPortal:   P44460
- SMR:   P44460
- GeneID:   950915
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0023
- NMPDR:   fig|71421.1.peg.22
- TIGR:   HI_0023
- HOGENOM:   HBG676713
- OMA:   DANNEEG
- ProtClustDB:   CLSK874112
- BioCyc:   HINF71421:HI_0023-MONOMER
- BRENDA:   4.1.3.34
- BRENDA:   4.1.3.6
- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PIRSF:   PIRSF015582
- TIGRFAMs:   TIGR01588

Pfam domain/function: PF03328 HpcH_HpaI; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.6; =4.1.3.34

Molecular weight: Translated: 31083; Mature: 31083

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFVPGSNAAMLSNSFIYKPDSIMFDLEDAVALKEKDSARLLVAHALQHPLYKEIETVVR
CEEECCCCCHHHCCCEEECCCCEEEEHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHC
VNPLDSEFGLLDLNSVVRAGVDVVRMPKTESAQDVLDMDHAITEIEKACGREAGSTKMLA
CCCCCCCCCEEEHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AIESPLGITQANQIAFASKRLIGIALGAEDYVRNLKTERSPEGIELLFARCSILQAARAA
HHHCCCCCCCHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
GIQAFDTVYSNANNEEGFLKEAALIKQLGFDGKSLINPRQIELLHNLFAPTQKDVEQAKR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHH
IIEAAVEAERQGAGVVSLNGKMIDAPIIDRAKLVLERAKSGIREE
HHHHHHHHHHCCCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLFVPGSNAAMLSNSFIYKPDSIMFDLEDAVALKEKDSARLLVAHALQHPLYKEIETVVR
CEEECCCCCHHHCCCEEECCCCEEEEHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHC
VNPLDSEFGLLDLNSVVRAGVDVVRMPKTESAQDVLDMDHAITEIEKACGREAGSTKMLA
CCCCCCCCCEEEHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AIESPLGITQANQIAFASKRLIGIALGAEDYVRNLKTERSPEGIELLFARCSILQAARAA
HHHCCCCCCCHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
GIQAFDTVYSNANNEEGFLKEAALIKQLGFDGKSLINPRQIELLHNLFAPTQKDVEQAKR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHH
IIEAAVEAERQGAGVVSLNGKMIDAPIIDRAKLVLERAKSGIREE
HHHHHHHHHHCCCCEEEECCEEECCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800