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The map label for this gene is pyk [H]

Identifier: 99082356

GI number: 99082356

Start: 2655276

End: 2656721

Strand: Reverse

Name: pyk [H]

Synonym: TM1040_2516

Alternate gene names: 99082356

Gene position: 2656721-2655276 (Counterclockwise)

Preceding gene: 99082357

Following gene: 99082355

Centisome position: 83.0

GC content: 60.17

Gene sequence:

>1446_bases
ATGAAACGCACGCGCAATGTCAAAATCGTGGCCACGCTCGGTCCTGCCTCCGAGACCTACGAGACCATTCGCGCTCTGCA
TGAGGCAGGTGCGGATGTGTTCCGCCTGAATATGTCCCACGGCAGCCACGATGAAATCGCGGTGAAGCACAAGATCATCC
GCCAGGTCGAAGAAGACCTGGACTCCACCATCGCCATTCTGGCCGACCTGCAGGGACCTAAACTCCGCGTCGGCGTGTTT
GCGAATGGTGCCGAGGAACTCGAAGAAGGGGCAAATTTCCGCCTTGATCTCGATCCGGCCGAGGGCGATCTGTCGCGCGT
TTGCCTGCCGCACCCCGAGATTTTTCAGGCGCTGGAGCCCGGTGCTTCGCTTCTGGTCAACGATGGCAAGATCCGTCTCA
AGGTGGAGGATTGCGGCGAGGATTATGCCAATTGCGTCGTGACCACCGGTGGCACCATCTCGAACCGCAAGGGTGTGAAT
GTTCCCGATGTGATCCTGCCGTTGGCGGCACTTTCGGAAAAAGACCGTGCGGATCTGGAGTTTGTCTGCGAGCTCGGTGT
CGACTGGCTGGCGCTGTCCTTCGTACAACGCGCAAAAGACGTCTTTGAGGCCCGCGCTCTGGCTGATGGGCGCGCCGCAA
TCCTCTCCAAGATCGAAAAGCCCGCCGCCGTCGACAACTTTGCAGAAATTCTGGATGCCTCCGATGGCATCATGGTAGCG
CGCGGAGATCTTGGCGTTGAGCTGCCGGTTTCGCATGTGCCGCCGATCCAGAAACGCCTGGTCCGCAAGAGCCGCGCGGC
CGCAAAGCCCGTGATTGTGGCGACACAGATGCTCGAGAGCATGATCGAGAGCCCGATGCCCACCCGTGCAGAGGTCTCTG
ACGTGGCAACCGCGATCTACGAGGGCACCGACGCTATCATGCTCAGCGCGGAGTCTGCAGCGGGTCAGTACCCTATCGAA
GCGGTTCAGACGATGGACAAGGTCGCAACCGAAGTGGAGCAGGACCCGACATATCGCGACATTATCGCCGCTTCCCGCTC
TTCCAAGGGTGACACCATCGCGGATGGCATCGTGGCAGCTGCGCGCGAGATTGCGGAGAAGACCGAGATCAAGGCGATCT
GCTGTTTTACACAGTCCGGAACCACCGCGCTTTTGACTGCGCGCGAGCGGCCCTATGTGCCGATCCTCGCGCTGACACCG
CTGGCAAAAACCGCCCGCCGCCTTTGCCTGACCTGGGGTTGCAACTGTGTGGTTACGCCCAAGGTGGATCGCTTCAAGGG
GGCTGTGGTATCTGCTGCGCGTGCCGCGCGTTCGGGTGGCTTTGCCTCTGAGACGGATCAGGTTGTCGTCACTGCCGGTG
TGCCGTTCAACGTACCGGGCACCACCAATATCTTGCGCATTGCGCCTTGTGATGAACGCCTGATTTACAATACCGACCCG
GAATAA

Upstream 100 bases:

>100_bases
ACGGTCTGTGTGAAGCTTTTGGTTGCATAGCGCCCTACCACGTAAATTCGTTATCAAGATTTGCCGTTCAAAAATGCGGC
TGCCGAAGGATGGAGATCCC

Downstream 100 bases:

>100_bases
TACCCTTTCCCCCGTATGTATTAGGGGGTGAGGCGTATGGACACGGACCTGTTATTGGTGACTGGGCTGATACTCGCCTG
GCTTGGTGTGCCTGCCTTCT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MKRTRNVKIVATLGPASETYETIRALHEAGADVFRLNMSHGSHDEIAVKHKIIRQVEEDLDSTIAILADLQGPKLRVGVF
ANGAEELEEGANFRLDLDPAEGDLSRVCLPHPEIFQALEPGASLLVNDGKIRLKVEDCGEDYANCVVTTGGTISNRKGVN
VPDVILPLAALSEKDRADLEFVCELGVDWLALSFVQRAKDVFEARALADGRAAILSKIEKPAAVDNFAEILDASDGIMVA
RGDLGVELPVSHVPPIQKRLVRKSRAAAKPVIVATQMLESMIESPMPTRAEVSDVATAIYEGTDAIMLSAESAAGQYPIE
AVQTMDKVATEVEQDPTYRDIIAASRSSKGDTIADGIVAAAREIAEKTEIKAICCFTQSGTTALLTARERPYVPILALTP
LAKTARRLCLTWGCNCVVTPKVDRFKGAVVSAARAARSGGFASETDQVVVTAGVPFNVPGTTNILRIAPCDERLIYNTDP
E

Sequences:

>Translated_481_residues
MKRTRNVKIVATLGPASETYETIRALHEAGADVFRLNMSHGSHDEIAVKHKIIRQVEEDLDSTIAILADLQGPKLRVGVF
ANGAEELEEGANFRLDLDPAEGDLSRVCLPHPEIFQALEPGASLLVNDGKIRLKVEDCGEDYANCVVTTGGTISNRKGVN
VPDVILPLAALSEKDRADLEFVCELGVDWLALSFVQRAKDVFEARALADGRAAILSKIEKPAAVDNFAEILDASDGIMVA
RGDLGVELPVSHVPPIQKRLVRKSRAAAKPVIVATQMLESMIESPMPTRAEVSDVATAIYEGTDAIMLSAESAAGQYPIE
AVQTMDKVATEVEQDPTYRDIIAASRSSKGDTIADGIVAAAREIAEKTEIKAICCFTQSGTTALLTARERPYVPILALTP
LAKTARRLCLTWGCNCVVTPKVDRFKGAVVSAARAARSGGFASETDQVVVTAGVPFNVPGTTNILRIAPCDERLIYNTDP
E
>Mature_481_residues
MKRTRNVKIVATLGPASETYETIRALHEAGADVFRLNMSHGSHDEIAVKHKIIRQVEEDLDSTIAILADLQGPKLRVGVF
ANGAEELEEGANFRLDLDPAEGDLSRVCLPHPEIFQALEPGASLLVNDGKIRLKVEDCGEDYANCVVTTGGTISNRKGVN
VPDVILPLAALSEKDRADLEFVCELGVDWLALSFVQRAKDVFEARALADGRAAILSKIEKPAAVDNFAEILDASDGIMVA
RGDLGVELPVSHVPPIQKRLVRKSRAAAKPVIVATQMLESMIESPMPTRAEVSDVATAIYEGTDAIMLSAESAAGQYPIE
AVQTMDKVATEVEQDPTYRDIIAASRSSKGDTIADGIVAAAREIAEKTEIKAICCFTQSGTTALLTARERPYVPILALTP
LAKTARRLCLTWGCNCVVTPKVDRFKGAVVSAARAARSGGFASETDQVVVTAGVPFNVPGTTNILRIAPCDERLIYNTDP
E

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=37.9591836734694, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=37.9591836734694, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=38.1632653061225, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=37.8048780487805, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=37.8048780487805, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=39.0243902439024, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=39.0243902439024, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=35.3191489361702, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=35.3191489361702, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI310128738, Length=212, Percent_Identity=34.4339622641509, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=40.0835073068894, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=39.2781316348195, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=37.1900826446281, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=37.5, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=37.5, Blast_Score=252, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=37.5, Blast_Score=252, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=37.5, Blast_Score=251, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=426, Percent_Identity=38.0281690140845, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=496, Percent_Identity=34.4758064516129, Blast_Score=229, Evalue=9e-61,
Organism=Drosophila melanogaster, GI24648964, Length=486, Percent_Identity=36.6255144032922, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=36.6255144032922, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=33.5390946502058, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24648966, Length=386, Percent_Identity=38.0829015544041, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24646914, Length=259, Percent_Identity=38.6100386100386, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51626; Mature: 51626

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTRNVKIVATLGPASETYETIRALHEAGADVFRLNMSHGSHDEIAVKHKIIRQVEEDL
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
DSTIAILADLQGPKLRVGVFANGAEELEEGANFRLDLDPAEGDLSRVCLPHPEIFQALEP
HHHEEHHEECCCCEEEEEEECCCHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCC
GASLLVNDGKIRLKVEDCGEDYANCVVTTGGTISNRKGVNVPDVILPLAALSEKDRADLE
CCEEEEECCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHH
FVCELGVDWLALSFVQRAKDVFEARALADGRAAILSKIEKPAAVDNFAEILDASDGIMVA
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
RGDLGVELPVSHVPPIQKRLVRKSRAAAKPVIVATQMLESMIESPMPTRAEVSDVATAIY
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EGTDAIMLSAESAAGQYPIEAVQTMDKVATEVEQDPTYRDIIAASRSSKGDTIADGIVAA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHH
AREIAEKTEIKAICCFTQSGTTALLTARERPYVPILALTPLAKTARRLCLTWGCNCVVTP
HHHHHHHHCEEEEEEEECCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHCCCCEEECC
KVDRFKGAVVSAARAARSGGFASETDQVVVTAGVPFNVPGTTNILRIAPCDERLIYNTDP
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEECCCC
E
C
>Mature Secondary Structure
MKRTRNVKIVATLGPASETYETIRALHEAGADVFRLNMSHGSHDEIAVKHKIIRQVEEDL
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
DSTIAILADLQGPKLRVGVFANGAEELEEGANFRLDLDPAEGDLSRVCLPHPEIFQALEP
HHHEEHHEECCCCEEEEEEECCCHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCC
GASLLVNDGKIRLKVEDCGEDYANCVVTTGGTISNRKGVNVPDVILPLAALSEKDRADLE
CCEEEEECCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHH
FVCELGVDWLALSFVQRAKDVFEARALADGRAAILSKIEKPAAVDNFAEILDASDGIMVA
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
RGDLGVELPVSHVPPIQKRLVRKSRAAAKPVIVATQMLESMIESPMPTRAEVSDVATAIY
ECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EGTDAIMLSAESAAGQYPIEAVQTMDKVATEVEQDPTYRDIIAASRSSKGDTIADGIVAA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHH
AREIAEKTEIKAICCFTQSGTTALLTARERPYVPILALTPLAKTARRLCLTWGCNCVVTP
HHHHHHHHCEEEEEEEECCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHCCCCEEECC
KVDRFKGAVVSAARAARSGGFASETDQVVVTAGVPFNVPGTTNILRIAPCDERLIYNTDP
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEECCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA