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The map label for this gene is sbp [H]

Identifier: 91783349

GI number: 91783349

Start: 2193007

End: 2194047

Strand: Direct

Name: sbp [H]

Synonym: Bxe_A2470

Alternate gene names: 91783349

Gene position: 2193007-2194047 (Clockwise)

Preceding gene: 91783345

Following gene: 91783350

Centisome position: 44.79

GC content: 63.11

Gene sequence:

>1041_bases
ATGAATCACCAAGGTACGGGGCTGGCGGGCAGGGCGAAGACACTCATCGTGGCGCTGGCACTGGGTGCGGCAGGCGCGCT
AGGCGTAATGGCGCAGGCACACGCTGCCGACACGACGCTGCTGAACGTGTCCTACGACCCGACGCGCGAGTTGTATCAGG
ACATCAACCAGGCGTTCGGCAAGCAATGGAAGGCGAAGACCGGCGACACGATCACCTTCAAGCAGTCGCACGGCGGCTCC
GGCGCGCAGGCGCGTTCGGTGCTCGACGGTCTGCAGGCCGACGTCGTGACGCTTGCGCTCGCTTACGACATCGACGCACT
GGCCAGCAAGGGGCTGCTGGACAAGGGCTGGCAGAAGCGTTTGCCGGATAACGCGTCGCCGTACACGTCGACGATCGTGT
TTCTGGTGCGCAAGGGCAACCCGAAGCACATCAAGGACTGGGATGATCTGATCAAGCCGGGTGTCTCGATCGTCACGCCG
AATCCGAAGACGTCGGGCGGCGCGCGCTGGAACTACCTCGCGGCCTGGGCGTACGCCGAGCATCAGCCGGGCGGCAATGA
CCAGAAGGCCAAGGAATTCGTCGGCAAGCTTTACAAGAATGCCGGCGTGCTGGATTCGGGCGCGCGCGGCGCGACTACCA
GCTTCGTGCAGCGCGGTATCGGCGACGTGCTGATCGCGTGGGAAAACGAAGCCTTCCTGTCGCTGAAGGAATTCGGTCCG
GACAAGTTCGAGATCGTCGTGCCGTCGGTCAGCATTCTGGCGGAGCCGCCGGTTGCGGTGGTGGACAAGGTGGTCGACAG
GCACGGCACGCGCAAGGTCGCCGAAGCGTATCTGAACTTCCTCTATAGCGAGGAAGGGCAGGAAATCGCCGCGAAGAACT
TCTACCGTCCGCGTTCGAACAAGGTGCCGGCCGAGCTGACCGCGAAGTTTCCGAAGCTGAAGCTGTACACGGTGGACGAC
TCGTTCGGCGGCTGGACCAACGCGCAGAAAACGCACTTTGCCGATGGCGGCGTGTTCGATTCGATCTACCAGCCGCAGTA
A

Upstream 100 bases:

>100_bases
TCGATAGACTGGCCTCCAGTTGGGGCTCATGTGCACGTGACCGCGACGCAGAGCAACACCACACGCTGTTTCAGCGAATT
ACAACGCGGAGAAAGTTGAC

Downstream 100 bases:

>100_bases
TACCGCCCGTGCCTGCAATGGCACAGGCAAAGCGACACGCTCACGCGTCACACCCAGGCGCGCCCCCAGGGGCGCGCTTT
TGGCCAGCCAGGGCCCGGCG

Product: ABC sulfate/thiosulfate transporter, periplasmic ligand binding protein, CysP

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNHQGTGLAGRAKTLIVALALGAAGALGVMAQAHAADTTLLNVSYDPTRELYQDINQAFGKQWKAKTGDTITFKQSHGGS
GAQARSVLDGLQADVVTLALAYDIDALASKGLLDKGWQKRLPDNASPYTSTIVFLVRKGNPKHIKDWDDLIKPGVSIVTP
NPKTSGGARWNYLAAWAYAEHQPGGNDQKAKEFVGKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSLKEFGP
DKFEIVVPSVSILAEPPVAVVDKVVDRHGTRKVAEAYLNFLYSEEGQEIAAKNFYRPRSNKVPAELTAKFPKLKLYTVDD
SFGGWTNAQKTHFADGGVFDSIYQPQ

Sequences:

>Translated_346_residues
MNHQGTGLAGRAKTLIVALALGAAGALGVMAQAHAADTTLLNVSYDPTRELYQDINQAFGKQWKAKTGDTITFKQSHGGS
GAQARSVLDGLQADVVTLALAYDIDALASKGLLDKGWQKRLPDNASPYTSTIVFLVRKGNPKHIKDWDDLIKPGVSIVTP
NPKTSGGARWNYLAAWAYAEHQPGGNDQKAKEFVGKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSLKEFGP
DKFEIVVPSVSILAEPPVAVVDKVVDRHGTRKVAEAYLNFLYSEEGQEIAAKNFYRPRSNKVPAELTAKFPKLKLYTVDD
SFGGWTNAQKTHFADGGVFDSIYQPQ
>Mature_346_residues
MNHQGTGLAGRAKTLIVALALGAAGALGVMAQAHAADTTLLNVSYDPTRELYQDINQAFGKQWKAKTGDTITFKQSHGGS
GAQARSVLDGLQADVVTLALAYDIDALASKGLLDKGWQKRLPDNASPYTSTIVFLVRKGNPKHIKDWDDLIKPGVSIVTP
NPKTSGGARWNYLAAWAYAEHQPGGNDQKAKEFVGKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSLKEFGP
DKFEIVVPSVSILAEPPVAVVDKVVDRHGTRKVAEAYLNFLYSEEGQEIAAKNFYRPRSNKVPAELTAKFPKLKLYTVDD
SFGGWTNAQKTHFADGGVFDSIYQPQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=342, Percent_Identity=64.9122807017544, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1788765, Length=311, Percent_Identity=49.1961414790997, Blast_Score=303, Evalue=8e-84,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37439; Mature: 37439

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQGTGLAGRAKTLIVALALGAAGALGVMAQAHAADTTLLNVSYDPTRELYQDINQAFG
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHC
KQWKAKTGDTITFKQSHGGSGAQARSVLDGLQADVVTLALAYDIDALASKGLLDKGWQKR
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHH
LPDNASPYTSTIVFLVRKGNPKHIKDWDDLIKPGVSIVTPNPKTSGGARWNYLAAWAYAE
CCCCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECC
HQPGGNDQKAKEFVGKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSLKEFGP
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEHHHCCC
DKFEIVVPSVSILAEPPVAVVDKVVDRHGTRKVAEAYLNFLYSEEGQEIAAKNFYRPRSN
CEEEEEECCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
KVPAELTAKFPKLKLYTVDDSFGGWTNAQKTHFADGGVFDSIYQPQ
CCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNHQGTGLAGRAKTLIVALALGAAGALGVMAQAHAADTTLLNVSYDPTRELYQDINQAFG
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHC
KQWKAKTGDTITFKQSHGGSGAQARSVLDGLQADVVTLALAYDIDALASKGLLDKGWQKR
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHH
LPDNASPYTSTIVFLVRKGNPKHIKDWDDLIKPGVSIVTPNPKTSGGARWNYLAAWAYAE
CCCCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCEEEEEEEEECC
HQPGGNDQKAKEFVGKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSLKEFGP
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEHHHCCC
DKFEIVVPSVSILAEPPVAVVDKVVDRHGTRKVAEAYLNFLYSEEGQEIAAKNFYRPRSN
CEEEEEECCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
KVPAELTAKFPKLKLYTVDDSFGGWTNAQKTHFADGGVFDSIYQPQ
CCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]