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The map label for this gene is cysU [H]

Identifier: 91783350

GI number: 91783350

Start: 2194186

End: 2195079

Strand: Direct

Name: cysU [H]

Synonym: Bxe_A2469

Alternate gene names: 91783350

Gene position: 2194186-2195079 (Clockwise)

Preceding gene: 91783349

Following gene: 91783351

Centisome position: 44.82

GC content: 64.54

Gene sequence:

>894_bases
ATGACGACGTTGACCTTCCGAAAACCGAGCGCGTTGCCCGGTTTTGGCCTGACACTCGGCATCACGGTGGCTTATCTGAG
CCTCGTGGTGCTGATTCCGCTTGCGGCGACCTTTCTGAAGACCGCGACGCTCGACTGGGAGCAGTTCGTGCGCGCGGTGA
GCTCACCGCGCGTGCTCGCGTCGTATCGCCTGACGTTCTTTTCCGCGCTCGGCGGCGCGCTCATCAATGCGGTGTTCGGC
TTTCTGGTGGCGTGGGTGTTGGTGCGCTACACGTTTCCGTTCAAACGCATCGTCGATGCCGTGGTGGATTTGCCGTTCGC
GCTGCCCACCTCGGTGGCCGGCATTTCGCTCGCGGCCGTGTACGCGGGCAACGGCTGGATCGGACAGTTCCTCGAACCGC
TCGGGCTGAAGATCGCGTTTACGCCGGCGGGCGTGCTGGTCGCGCTGACGTTTATCGGTCTGCCGTTCGTGGTGCGCACG
GTGCAGCCGGTGCTCGAAGAGTTCGAGCGCGAACAGGAGGAAGCGGCGGCCTGCCTTGGCGCGTCGCGCTGGCTGACGTT
TCGCCGCGTAGTGTTGCCGGCGGTGTTTCCGGCGCTGTTGACGGGCTTCGCGCTGGCATTCGCCCGCGCGCTCGGTGAAT
ACGGTTCGGTGATCTTCATCGCCGGTAACGTGCCGATGAAATCGGAAATCACCTCGCTGCTGATCATCACGAAGCTCGAG
CAATACGACTACGCGGGCGCAACGGCGCTGGCGGTGGTGATGCTGGTCGTGTCGTTCCTGATGCTGCTCCTGATCAATAC
CTTGCAGTGGTATCTGCAGCGCCGCACGAGCCGCGGCGGCGCGGGTCCGGCGCCTGCCGCCAGTGTTGCGGCCGTCGGCG
GAGGTGTGCAATGA

Upstream 100 bases:

>100_bases
GCTCACGCGTCACACCCAGGCGCGCCCCCAGGGGCGCGCTTTTGGCCAGCCAGGGCCCGGCGGCCGGTAACACGAGCATC
AACCTGAACGAGTAACGAGC

Downstream 100 bases:

>100_bases
GCCGCGGCACCGCGAATGGAGCGGATGACGTGAATGGCGTGCGCGGCGTGAATAACGCCGCGGTGGCCGTGGCGCCGCGC
GCACCGTTGAACGTTGCGCG

Product: ABC sulfate/thiosulfate transporter, innermembrane subunit, CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTTLTFRKPSALPGFGLTLGITVAYLSLVVLIPLAATFLKTATLDWEQFVRAVSSPRVLASYRLTFFSALGGALINAVFG
FLVAWVLVRYTFPFKRIVDAVVDLPFALPTSVAGISLAAVYAGNGWIGQFLEPLGLKIAFTPAGVLVALTFIGLPFVVRT
VQPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGGAGPAPAASVAAVGGGVQ

Sequences:

>Translated_297_residues
MTTLTFRKPSALPGFGLTLGITVAYLSLVVLIPLAATFLKTATLDWEQFVRAVSSPRVLASYRLTFFSALGGALINAVFG
FLVAWVLVRYTFPFKRIVDAVVDLPFALPTSVAGISLAAVYAGNGWIGQFLEPLGLKIAFTPAGVLVALTFIGLPFVVRT
VQPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGGAGPAPAASVAAVGGGVQ
>Mature_296_residues
TTLTFRKPSALPGFGLTLGITVAYLSLVVLIPLAATFLKTATLDWEQFVRAVSSPRVLASYRLTFFSALGGALINAVFGF
LVAWVLVRYTFPFKRIVDAVVDLPFALPTSVAGISLAAVYAGNGWIGQFLEPLGLKIAFTPAGVLVALTFIGLPFVVRTV
QPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLEQ
YDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGGAGPAPAASVAAVGGGVQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=49.2424242424242, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI87082099, Length=262, Percent_Identity=34.3511450381679, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1786980, Length=202, Percent_Identity=33.6633663366337, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786254, Length=211, Percent_Identity=29.8578199052133, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787369, Length=151, Percent_Identity=29.1390728476821, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31753; Mature: 31622

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTFRKPSALPGFGLTLGITVAYLSLVVLIPLAATFLKTATLDWEQFVRAVSSPRVLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
SYRLTFFSALGGALINAVFGFLVAWVLVRYTFPFKRIVDAVVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHEE
YAGNGWIGQFLEPLGLKIAFTPAGVLVALTFIGLPFVVRTVQPVLEEFEREQEEAAACLG
EECCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGGAGPAPAASVAAVGGGVQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
TTLTFRKPSALPGFGLTLGITVAYLSLVVLIPLAATFLKTATLDWEQFVRAVSSPRVLA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
SYRLTFFSALGGALINAVFGFLVAWVLVRYTFPFKRIVDAVVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHEE
YAGNGWIGQFLEPLGLKIAFTPAGVLVALTFIGLPFVVRTVQPVLEEFEREQEEAAACLG
EECCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATALAVVMLVVSFLMLLLINTLQWYLQRRTSRGGAGPAPAASVAAVGGGVQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]