BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is braG [H]

Identifier: 86749171

GI number: 86749171

Start: 2328025

End: 2328765

Strand: Direct

Name: braG [H]

Synonym: RPB_2049

Alternate gene names: 86749171

Gene position: 2328025-2328765 (Clockwise)

Preceding gene: 86749170

Following gene: 86749172

Centisome position: 43.66

GC content: 67.07

Gene sequence:

>741_bases
ATGAGTATGCCCGAGACGGCCACGGCGCGCCCCGCGGTGCTCGACGTCAAGGGACTATCCGCCGGCTGGGGACCGATGCG
GGTGATCCACGACCTCGATCTCACCGTCTTCGCCGGCGAGCGGATCGGTCTGGTCGGCCTCAACGGCCACGGCAAGTCGA
CGTTGTTTCAGGCGATCGCCGGGCTCACCGGCTGGCAGCGCGGATCGATCAAGCTCAACGGCTATGAGGTCGGCCGCACC
CGCAGCCAGGGGCCGGGCCGCTACACGCATCTAATTGTGCGGAGAGGTCTGGCGCTGATCCCGCAGGGCGATGAGATCTT
TCACGGCCTGACGGTCGAGGAGCATCTCGACAGCGGCGCCTTCACCAAGAGCGCCTGGGCCGAGCGCGGCATCCGCAAGA
AGCGAATTCTGCAGATCTTCCCGCCGCTGGAGAAGCTGATGAAGATTCCGGTGGGCCGCCTGTCGGGCGGCGAGCGACGG
ATGGTGTCGATCGGCCGCGGCCTGATGTCCGACGCCGAACTGTTCCTGGTCGACGAGCCGTCGCTCGGGCTGGCGCCGAA
GATCGGCCGCTCGGTGGTCGATGCCTTGATGGCGGTCGAGCTCGGCGGCTCGGCGATGGTGATCGCCGAACAGAACGTCG
CGCTGCTCGAAGGCAGCGTCGATCGCATCATCGGAATGCACGCCGGCAAGCTGAAGGGCGAGGCCTCGGCCTCCAAGTCC
ATGTCGACGGAGCGCAGCTGA

Upstream 100 bases:

>100_bases
CCGGCGAGACGATCGCCGAAGGATCGCTTGCGGAGATCCTGCTCAACCCGCGCGTCAAGGCTGCGTATTTCGAGACCGAT
GCCGGCGGGGGGCTGCATTG

Downstream 100 bases:

>100_bases
TGAATTGGGGTTTCATCGTCACCAACGCGATCACGCTGGCCTGCATCTACGGGACGCTGGCGATCGGGATTTCGGTCACC
TGGTCGAGCCTCGGCCTGGT

Product: ABC transporter related

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSMPETATARPAVLDVKGLSAGWGPMRVIHDLDLTVFAGERIGLVGLNGHGKSTLFQAIAGLTGWQRGSIKLNGYEVGRT
RSQGPGRYTHLIVRRGLALIPQGDEIFHGLTVEEHLDSGAFTKSAWAERGIRKKRILQIFPPLEKLMKIPVGRLSGGERR
MVSIGRGLMSDAELFLVDEPSLGLAPKIGRSVVDALMAVELGGSAMVIAEQNVALLEGSVDRIIGMHAGKLKGEASASKS
MSTERS

Sequences:

>Translated_246_residues
MSMPETATARPAVLDVKGLSAGWGPMRVIHDLDLTVFAGERIGLVGLNGHGKSTLFQAIAGLTGWQRGSIKLNGYEVGRT
RSQGPGRYTHLIVRRGLALIPQGDEIFHGLTVEEHLDSGAFTKSAWAERGIRKKRILQIFPPLEKLMKIPVGRLSGGERR
MVSIGRGLMSDAELFLVDEPSLGLAPKIGRSVVDALMAVELGGSAMVIAEQNVALLEGSVDRIIGMHAGKLKGEASASKS
MSTERS
>Mature_245_residues
SMPETATARPAVLDVKGLSAGWGPMRVIHDLDLTVFAGERIGLVGLNGHGKSTLFQAIAGLTGWQRGSIKLNGYEVGRTR
SQGPGRYTHLIVRRGLALIPQGDEIFHGLTVEEHLDSGAFTKSAWAERGIRKKRILQIFPPLEKLMKIPVGRLSGGERRM
VSIGRGLMSDAELFLVDEPSLGLAPKIGRSVVDALMAVELGGSAMVIAEQNVALLEGSVDRIIGMHAGKLKGEASASKSM
STERS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI150417984, Length=213, Percent_Identity=28.6384976525822, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI47078218, Length=212, Percent_Identity=27.8301886792453, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI116734710, Length=210, Percent_Identity=30, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI45446740, Length=210, Percent_Identity=27.6190476190476, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI31657092, Length=212, Percent_Identity=28.3018867924528, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27881501, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI30795238, Length=220, Percent_Identity=26.8181818181818, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI27436953, Length=210, Percent_Identity=27.6190476190476, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI89111135, Length=228, Percent_Identity=25.4385964912281, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI153792144, Length=228, Percent_Identity=24.5614035087719, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI105990541, Length=200, Percent_Identity=25.5, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI27262624, Length=161, Percent_Identity=26.7080745341615, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI27262626, Length=161, Percent_Identity=26.7080745341615, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI21536376, Length=200, Percent_Identity=26.5, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87082268, Length=203, Percent_Identity=34.4827586206897, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1789672, Length=241, Percent_Identity=25.7261410788382, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789593, Length=238, Percent_Identity=29.8319327731092, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787029, Length=221, Percent_Identity=26.2443438914027, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082267, Length=180, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790467, Length=224, Percent_Identity=26.7857142857143, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788225, Length=235, Percent_Identity=25.9574468085106, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787089, Length=239, Percent_Identity=25.1046025104602, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1790190, Length=222, Percent_Identity=26.1261261261261, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790544, Length=246, Percent_Identity=28.8617886178862, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994943, Length=234, Percent_Identity=25.6410256410256, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787792, Length=229, Percent_Identity=27.0742358078603, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786872, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790525, Length=220, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87081791, Length=232, Percent_Identity=29.3103448275862, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788897, Length=235, Percent_Identity=26.3829787234043, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787370, Length=230, Percent_Identity=27.8260869565217, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787105, Length=216, Percent_Identity=25, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789991, Length=244, Percent_Identity=24.1803278688525, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87081782, Length=241, Percent_Identity=30.2904564315353, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786563, Length=170, Percent_Identity=31.7647058823529, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788761, Length=229, Percent_Identity=24.0174672489083, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87081834, Length=211, Percent_Identity=27.4881516587678, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786253, Length=228, Percent_Identity=27.6315789473684, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=232, Percent_Identity=30.6034482758621, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=232, Percent_Identity=30.6034482758621, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=232, Percent_Identity=30.6034482758621, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=165, Percent_Identity=31.5151515151515, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988343, Length=177, Percent_Identity=28.8135593220339, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=226, Percent_Identity=26.5486725663717, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24666092, Length=226, Percent_Identity=26.5486725663717, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648314, Length=218, Percent_Identity=27.5229357798165, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650853, Length=222, Percent_Identity=29.2792792792793, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24650855, Length=222, Percent_Identity=29.2792792792793, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362751, Length=226, Percent_Identity=28.3185840707965, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24643674, Length=232, Percent_Identity=26.7241379310345, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221512771, Length=237, Percent_Identity=25.7383966244726, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582014, Length=224, Percent_Identity=28.125, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24582012, Length=224, Percent_Identity=28.125, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI85725262, Length=247, Percent_Identity=29.1497975708502, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26316; Mature: 26185

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMPETATARPAVLDVKGLSAGWGPMRVIHDLDLTVFAGERIGLVGLNGHGKSTLFQAIA
CCCCCCCCCCCEEEEECCCCCCCCHHEEEEECCEEEEECCEEEEEEECCCCHHHHHHHHH
GLTGWQRGSIKLNGYEVGRTRSQGPGRYTHLIVRRGLALIPQGDEIFHGLTVEEHLDSGA
HCCCCCCCCEEECCEEECCCCCCCCCHHHHHHHHCCEEECCCCCHHHCCCCHHHHCCCCC
FTKSAWAERGIRKKRILQIFPPLEKLMKIPVGRLSGGERRMVSIGRGLMSDAELFLVDEP
CHHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECC
SLGLAPKIGRSVVDALMAVELGGSAMVIAEQNVALLEGSVDRIIGMHAGKLKGEASASKS
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHHHHHHCCCCCCCCCCHHHHC
MSTERS
CCCCCC
>Mature Secondary Structure 
SMPETATARPAVLDVKGLSAGWGPMRVIHDLDLTVFAGERIGLVGLNGHGKSTLFQAIA
CCCCCCCCCCEEEEECCCCCCCCHHEEEEECCEEEEECCEEEEEEECCCCHHHHHHHHH
GLTGWQRGSIKLNGYEVGRTRSQGPGRYTHLIVRRGLALIPQGDEIFHGLTVEEHLDSGA
HCCCCCCCCEEECCEEECCCCCCCCCHHHHHHHHCCEEECCCCCHHHCCCCHHHHCCCCC
FTKSAWAERGIRKKRILQIFPPLEKLMKIPVGRLSGGERRMVSIGRGLMSDAELFLVDEP
CHHHHHHHCCCCHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECC
SLGLAPKIGRSVVDALMAVELGGSAMVIAEQNVALLEGSVDRIIGMHAGKLKGEASASKS
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEECCCHHHHHHCCCCCCCCCCHHHHC
MSTERS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]