BacMap

Definition	Rhodopseudomonas palustris HaA2, complete genome.
Accession	NC_007778
Length	5,331,656

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The map label for this gene is braF [H]

Identifier: 86749170

GI number: 86749170

Start: 2327288

End: 2328025

Strand: Direct

Name: braF [H]

Synonym: RPB_2048

Alternate gene names: 86749170

Gene position: 2327288-2328025 (Clockwise)

Preceding gene: 86749169

Following gene: 86749171

Centisome position: 43.65

GC content: 65.85

Gene sequence:

>738_bases
ATGGCCGCATCCGCGATCTTCTCCTGCGATCAGATCGGTCTCGATCTCGGCGGACGTTCGATCCTGCGCGGCATTTCGCT
CGACGTGAAGCCGGGCGAGGTGCTGGGGATCATCGGGCCGAACGGCGCCGGCAAGACCAGCCTGTTCGAAGTGCTGTCGG
GGCGCATCTCGCCCAAGAGCGGAACGGTCGCCTATGGCGGCCGCGAGATCACCGGCCTGCCGCTGCACCGGCGGGCGCGT
CTCGGCATCGGCCGTACCTACCAGACCCCGATCGTGCCGGAAGACATGACCGTCGCGCAGGTTCTGAAAGCGGCGCGGCA
GGCGTATCGGCCGTATCTGACGCCCGACGACGCCGAATGGGGCGCGGGGCTGGTCGGCTTCCGGATTCCGGGCGCGATGA
TGGCGGATCGGCTCGACACGCTGAACCGCCGCAAACTGCTGATTGCCTGTCTGCTGATGCGGCGGCCGTCGGTGCTGCTG
ATGGACGAGCCGGCCGCGGGATTGATCAATTCCGAGATCGACGAACTCGACGACATCATGCGGGTGCTCGCCAAGGAACT
GAACATCGCGATCGTGCTGATCGAGCACCGGATGGAGCTGCTCGATACGCTTGCCGATCGGGTGCTGGTGATGGATGCCG
GCGAGACGATCGCCGAAGGATCGCTTGCGGAGATCCTGCTCAACCCGCGCGTCAAGGCTGCGTATTTCGAGACCGATGCC
GGCGGGGGGCTGCATTGA

Upstream 100 bases:

>100_bases
TTCCAGGTCCAGAACAACGAGCACAAGATCATCTATCCGGACGTGCTCGTCGAGAACAAGCTGCAGAAGGCGCCGTGGTG
GTGACGCCGGGTCGATGACG

Downstream 100 bases:

>100_bases
TGAGTATGCCCGAGACGGCCACGGCGCGCCCCGCGGTGCTCGACGTCAAGGGACTATCCGCCGGCTGGGGACCGATGCGG
GTGATCCACGACCTCGATCT

Product: ABC transporter related

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MAASAIFSCDQIGLDLGGRSILRGISLDVKPGEVLGIIGPNGAGKTSLFEVLSGRISPKSGTVAYGGREITGLPLHRRAR
LGIGRTYQTPIVPEDMTVAQVLKAARQAYRPYLTPDDAEWGAGLVGFRIPGAMMADRLDTLNRRKLLIACLLMRRPSVLL
MDEPAAGLINSEIDELDDIMRVLAKELNIAIVLIEHRMELLDTLADRVLVMDAGETIAEGSLAEILLNPRVKAAYFETDA
GGGLH

Sequences:

>Translated_245_residues
MAASAIFSCDQIGLDLGGRSILRGISLDVKPGEVLGIIGPNGAGKTSLFEVLSGRISPKSGTVAYGGREITGLPLHRRAR
LGIGRTYQTPIVPEDMTVAQVLKAARQAYRPYLTPDDAEWGAGLVGFRIPGAMMADRLDTLNRRKLLIACLLMRRPSVLL
MDEPAAGLINSEIDELDDIMRVLAKELNIAIVLIEHRMELLDTLADRVLVMDAGETIAEGSLAEILLNPRVKAAYFETDA
GGGLH
>Mature_244_residues
AASAIFSCDQIGLDLGGRSILRGISLDVKPGEVLGIIGPNGAGKTSLFEVLSGRISPKSGTVAYGGREITGLPLHRRARL
GIGRTYQTPIVPEDMTVAQVLKAARQAYRPYLTPDDAEWGAGLVGFRIPGAMMADRLDTLNRRKLLIACLLMRRPSVLLM
DEPAAGLINSEIDELDDIMRVLAKELNIAIVLIEHRMELLDTLADRVLVMDAGETIAEGSLAEILLNPRVKAAYFETDAG
GGLH

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI11967969, Length=232, Percent_Identity=29.3103448275862, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI116734710, Length=222, Percent_Identity=28.3783783783784, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI105990541, Length=210, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4505771, Length=240, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI9961252, Length=240, Percent_Identity=27.0833333333333, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI27477115, Length=217, Percent_Identity=27.6497695852535, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI27436953, Length=207, Percent_Identity=25.6038647342995, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI42741659, Length=240, Percent_Identity=26.25, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI153792144, Length=207, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=32.6693227091633, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1790525, Length=227, Percent_Identity=32.5991189427313, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994997, Length=218, Percent_Identity=31.651376146789, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789593, Length=239, Percent_Identity=29.7071129707113, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788165, Length=235, Percent_Identity=25.531914893617, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1790190, Length=240, Percent_Identity=27.5, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787089, Length=235, Percent_Identity=32.3404255319149, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787500, Length=222, Percent_Identity=26.1261261261261, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1788210, Length=212, Percent_Identity=30.6603773584906, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786803, Length=235, Percent_Identity=27.2340425531915, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786345, Length=236, Percent_Identity=26.6949152542373, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790739, Length=243, Percent_Identity=26.3374485596708, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48994883, Length=227, Percent_Identity=25.9911894273128, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788761, Length=209, Percent_Identity=29.1866028708134, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1786698, Length=219, Percent_Identity=25.5707762557078, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48995001, Length=228, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789672, Length=233, Percent_Identity=25.7510729613734, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788897, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787972, Length=240, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787792, Length=218, Percent_Identity=27.5229357798165, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787370, Length=226, Percent_Identity=24.7787610619469, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786319, Length=238, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082268, Length=240, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787758, Length=218, Percent_Identity=29.3577981651376, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788472, Length=211, Percent_Identity=27.4881516587678, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI145693107, Length=230, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081791, Length=225, Percent_Identity=24.8888888888889, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081782, Length=202, Percent_Identity=26.7326732673267, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786253, Length=212, Percent_Identity=25.4716981132075, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788506, Length=228, Percent_Identity=22.8070175438596, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789586, Length=229, Percent_Identity=26.6375545851528, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994943, Length=221, Percent_Identity=29.8642533936652, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787712, Length=214, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789891, Length=234, Percent_Identity=26.0683760683761, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789751, Length=213, Percent_Identity=27.6995305164319, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786703, Length=209, Percent_Identity=26.7942583732057, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788450, Length=227, Percent_Identity=24.6696035242291, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI87081834, Length=209, Percent_Identity=27.2727272727273, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1789032, Length=233, Percent_Identity=27.8969957081545, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=218, Percent_Identity=27.5229357798165, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146777, Length=237, Percent_Identity=27.0042194092827, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17565586, Length=206, Percent_Identity=28.6407766990291, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17541710, Length=235, Percent_Identity=28.0851063829787, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=199, Percent_Identity=28.643216080402, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193202349, Length=221, Percent_Identity=23.9819004524887, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320874, Length=192, Percent_Identity=27.6041666666667, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321121, Length=197, Percent_Identity=23.3502538071066, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24643674, Length=218, Percent_Identity=27.5229357798165, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45552337, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552335, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552339, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552347, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552363, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552351, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552345, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552355, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583969, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552361, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552341, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552343, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552359, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552357, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552353, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552349, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550972, Length=240, Percent_Identity=31.25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221512771, Length=208, Percent_Identity=31.25, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI85724864, Length=216, Percent_Identity=28.2407407407407, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580555, Length=216, Percent_Identity=28.2407407407407, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI85816269, Length=216, Percent_Identity=28.2407407407407, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580553, Length=216, Percent_Identity=28.2407407407407, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666092, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648314, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574533, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281363262, Length=221, Percent_Identity=25.3393665158371, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24653245, Length=221, Percent_Identity=25.3393665158371, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26355; Mature: 26224

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASAIFSCDQIGLDLGGRSILRGISLDVKPGEVLGIIGPNGAGKTSLFEVLSGRISPKS
CCCCHHCCHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
GTVAYGGREITGLPLHRRARLGIGRTYQTPIVPEDMTVAQVLKAARQAYRPYLTPDDAEW
CEEEECCEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GAGLVGFRIPGAMMADRLDTLNRRKLLIACLLMRRPSVLLMDEPAAGLINSEIDELDDIM
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
RVLAKELNIAIVLIEHRMELLDTLADRVLVMDAGETIAEGSLAEILLNPRVKAAYFETDA
HHHHHHCCEEEEEHHHHHHHHHHHHHHEEEEECCCHHHCCCHHHHHCCCCEEEEEEECCC
GGGLH
CCCCC
>Mature Secondary Structure 
AASAIFSCDQIGLDLGGRSILRGISLDVKPGEVLGIIGPNGAGKTSLFEVLSGRISPKS
CCCHHCCHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
GTVAYGGREITGLPLHRRARLGIGRTYQTPIVPEDMTVAQVLKAARQAYRPYLTPDDAEW
CEEEECCEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GAGLVGFRIPGAMMADRLDTLNRRKLLIACLLMRRPSVLLMDEPAAGLINSEIDELDDIM
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
RVLAKELNIAIVLIEHRMELLDTLADRVLVMDAGETIAEGSLAEILLNPRVKAAYFETDA
HHHHHHCCEEEEEHHHHHHHHHHHHHHEEEEECCCHHHCCCHHHHHCCCCEEEEEEECCC
GGGLH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]