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The map label for this gene is cysW [H]

Identifier: 86748521

GI number: 86748521

Start: 1587138

End: 1588004

Strand: Reverse

Name: cysW [H]

Synonym: RPB_1396

Alternate gene names: 86748521

Gene position: 1588004-1587138 (Counterclockwise)

Preceding gene: 86748522

Following gene: 86748520

Centisome position: 29.78

GC content: 66.67

Gene sequence:

>867_bases
ATGGCGAAGCCGCAGCCCCCGATCAAGAGAGACTGGATGGTAACGCCGGTCGGCGCCGGGCCGGTCGCACGCTTCGTCGT
GCTGAGCGTGGTCGCCGTGGTGACGCTGTTCATTCTGGTGGCGCCGCTGGCGGTGATCCTGAGTTCGGCCTTCGCGCAGG
GCGTCGGCGTGTTCCTGCGCAATCTCGGCGATCCCGGCACGCTGCACGCGATGTGGCTGACCACGATCACCGCGCTGATC
GCGGTACCGGTGAACATCCTGTTCGGGCTCGCTGCGGCCTGGACCGTGACCAAGTTCGAATTCCCCGGCCGCACACTGCT
GATCGCGCTGATCGAGCTGCCCTATTCGATCTCGCCGATCGTCGCCGGCGTCGCCTATCTGTTCGTCTACGGCTCGCAGG
GGCTGTTCGGGCCGCTGCTGGAACAGCTCGACATCAAGGTGATGTTCGCGCTGCCGGGCATCGTGCTCGCCAGCATGTTC
GTCACCGCGCCCTATGTCGCCCGCGAGTTGATCCCGCTGATGCAGGTGCAGGGCACCGACGAGGAAGAGGCAGCGGTGAC
GCTCGGCGCCGGCGGCTTCGCCACCTTCCTGCGCGTCACGCTGCCGAATATCCGCTGGGCGGTGCTGTACGGCGCGATCC
TATGCAACGCCCGGGTGCTGGGCGAATTCGGCGCGGTGTCGGTGGTGTCCGGCAACGTCCGCGGCCAGACCACGACGCTG
CCGCTGCAGATCGAACTGCTGTACCAGGACTATAATGTCGCAGGCGCGTTCGCCGCCGCGACCACATTGACGGCGGTGGC
GGTGGTCACCATCCTGCTGAAAATGCTGCTGGAGCGGCTGGCCGGCGACGAGCATCCTTCGGCTTAG

Upstream 100 bases:

>100_bases
CGATCGCGGTGGTGATGCTGGTGATGGCGTTCGCGATGCTGCTCGTGACCAATGCGGTGCAGGCCTGGCAATTGCGCTAT
CTCGGACGGGGTGACAGGTG

Downstream 100 bases:

>100_bases
GTCGCGGCTGGCGCGAGGGGCCTTCCAATGCGCTCCGCTTTGGCGCTAAAAGAGGCACCCCCGAGGCCGCCATCCGCGGT
CGTCGGCTCATTTTGACCCC

Product: sulfate ABC transporter, permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAKPQPPIKRDWMVTPVGAGPVARFVVLSVVAVVTLFILVAPLAVILSSAFAQGVGVFLRNLGDPGTLHAMWLTTITALI
AVPVNILFGLAAAWTVTKFEFPGRTLLIALIELPYSISPIVAGVAYLFVYGSQGLFGPLLEQLDIKVMFALPGIVLASMF
VTAPYVARELIPLMQVQGTDEEEAAVTLGAGGFATFLRVTLPNIRWAVLYGAILCNARVLGEFGAVSVVSGNVRGQTTTL
PLQIELLYQDYNVAGAFAAATTLTAVAVVTILLKMLLERLAGDEHPSA

Sequences:

>Translated_288_residues
MAKPQPPIKRDWMVTPVGAGPVARFVVLSVVAVVTLFILVAPLAVILSSAFAQGVGVFLRNLGDPGTLHAMWLTTITALI
AVPVNILFGLAAAWTVTKFEFPGRTLLIALIELPYSISPIVAGVAYLFVYGSQGLFGPLLEQLDIKVMFALPGIVLASMF
VTAPYVARELIPLMQVQGTDEEEAAVTLGAGGFATFLRVTLPNIRWAVLYGAILCNARVLGEFGAVSVVSGNVRGQTTTL
PLQIELLYQDYNVAGAFAAATTLTAVAVVTILLKMLLERLAGDEHPSA
>Mature_287_residues
AKPQPPIKRDWMVTPVGAGPVARFVVLSVVAVVTLFILVAPLAVILSSAFAQGVGVFLRNLGDPGTLHAMWLTTITALIA
VPVNILFGLAAAWTVTKFEFPGRTLLIALIELPYSISPIVAGVAYLFVYGSQGLFGPLLEQLDIKVMFALPGIVLASMFV
TAPYVARELIPLMQVQGTDEEEAAVTLGAGGFATFLRVTLPNIRWAVLYGAILCNARVLGEFGAVSVVSGNVRGQTTTLP
LQIELLYQDYNVAGAFAAATTLTAVAVVTILLKMLLERLAGDEHPSA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=270, Percent_Identity=52.5925925925926, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788764, Length=200, Percent_Identity=37, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786980, Length=225, Percent_Identity=31.1111111111111, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30505; Mature: 30373

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPQPPIKRDWMVTPVGAGPVARFVVLSVVAVVTLFILVAPLAVILSSAFAQGVGVFLR
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLGDPGTLHAMWLTTITALIAVPVNILFGLAAAWTVTKFEFPGRTLLIALIELPYSISPI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCHHHH
VAGVAYLFVYGSQGLFGPLLEQLDIKVMFALPGIVLASMFVTAPYVARELIPLMQVQGTD
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEEAAVTLGAGGFATFLRVTLPNIRWAVLYGAILCNARVLGEFGAVSVVSGNVRGQTTTL
CCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEE
PLQIELLYQDYNVAGAFAAATTLTAVAVVTILLKMLLERLAGDEHPSA
EEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKPQPPIKRDWMVTPVGAGPVARFVVLSVVAVVTLFILVAPLAVILSSAFAQGVGVFLR
CCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLGDPGTLHAMWLTTITALIAVPVNILFGLAAAWTVTKFEFPGRTLLIALIELPYSISPI
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCHHHH
VAGVAYLFVYGSQGLFGPLLEQLDIKVMFALPGIVLASMFVTAPYVARELIPLMQVQGTD
HHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEEAAVTLGAGGFATFLRVTLPNIRWAVLYGAILCNARVLGEFGAVSVVSGNVRGQTTTL
CCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEE
PLQIELLYQDYNVAGAFAAATTLTAVAVVTILLKMLLERLAGDEHPSA
EEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]