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The map label for this gene is cysU [H]

Identifier: 86748522

GI number: 86748522

Start: 1588004

End: 1588831

Strand: Reverse

Name: cysU [H]

Synonym: RPB_1397

Alternate gene names: 86748522

Gene position: 1588831-1588004 (Counterclockwise)

Preceding gene: 86748523

Following gene: 86748521

Centisome position: 29.8

GC content: 66.55

Gene sequence:

>828_bases
TTGCGACGCAGTGTCATTCCCGGCTTCGGTCTCAGTCTCGGCATCACCGTGCTTTATCTCGGCGTGATCGTGCTGCTGCC
GCTGTGCGCGCTGATCCTGAAGGCCTCCGACATCGGCGCGGCCCAGCTCTGGGCGATCCTGTCGTCGCCGCGGACGCTTG
CCGCCATCAGGCTGACGGTGACGATGGCGCTCGCCGCCACGATCTTCAACGCGGTCTACGGCCTGCTGCTGGCGTGGGTA
CTGGCGCGCTATCAGTTCCCCGGCAAGCGGCTGCTCGATGCGCTGGTCGACGTGCCGTTCGCGCTGCCGACGGCGGTGGC
AGGCCTGGCGCTGACGGCGTTGTTCTCGAAGACCGGCTGGTTTGGGTCCGTGCTGGAGCCGCTCGGCGTCAGCGTCGTCT
ACACCCCGCTCGGCATCGGCATCGCGATGGCGTTCACCAGCATTCCGTTCGTGGTGCGCACCGTGCAGCCGGTGATCGAG
GATCTCGGCAGCGACGTCGAGGAGGCCAGCCGCTCGCTCGGCGCCAGCGATCTGCAGATCCTGTCGAAGATCATCTTTCC
GTCGATCTTCCCGGCGCTGCTGGCCGGCGCGTCGCTGGCCTTCGCGCGCAGCCTCGGCGAATTCGGCGCCGTGATCTTCA
TCGCCGGCAATCAGCCGATGAAGACCGAGATCGTCGCGCTGCTCGCCTTCATCCGGCTCGAGGAATACGACTATCCGGCC
GCCGCGACGATCGCGGTGGTGATGCTGGTGATGGCGTTCGCGATGCTGCTCGTGACCAATGCGGTGCAGGCCTGGCAATT
GCGCTATCTCGGACGGGGTGACAGGTGA

Upstream 100 bases:

>100_bases
GCCTCGCGGAGACGCCCCCTCACCCCACCCCTCTCCCCGCACGCGGGGAGAGGGAGCATGCCGCACTCGCGGTTACTTCA
TCCATTCACAGGTAACTTCT

Downstream 100 bases:

>100_bases
TGGCGAAGCCGCAGCCCCCGATCAAGAGAGACTGGATGGTAACGCCGGTCGGCGCCGGGCCGGTCGCACGCTTCGTCGTG
CTGAGCGTGGTCGCCGTGGT

Product: sulfate ABC transporter, permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRRSVIPGFGLSLGITVLYLGVIVLLPLCALILKASDIGAAQLWAILSSPRTLAAIRLTVTMALAATIFNAVYGLLLAWV
LARYQFPGKRLLDALVDVPFALPTAVAGLALTALFSKTGWFGSVLEPLGVSVVYTPLGIGIAMAFTSIPFVVRTVQPVIE
DLGSDVEEASRSLGASDLQILSKIIFPSIFPALLAGASLAFARSLGEFGAVIFIAGNQPMKTEIVALLAFIRLEEYDYPA
AATIAVVMLVMAFAMLLVTNAVQAWQLRYLGRGDR

Sequences:

>Translated_275_residues
MRRSVIPGFGLSLGITVLYLGVIVLLPLCALILKASDIGAAQLWAILSSPRTLAAIRLTVTMALAATIFNAVYGLLLAWV
LARYQFPGKRLLDALVDVPFALPTAVAGLALTALFSKTGWFGSVLEPLGVSVVYTPLGIGIAMAFTSIPFVVRTVQPVIE
DLGSDVEEASRSLGASDLQILSKIIFPSIFPALLAGASLAFARSLGEFGAVIFIAGNQPMKTEIVALLAFIRLEEYDYPA
AATIAVVMLVMAFAMLLVTNAVQAWQLRYLGRGDR
>Mature_275_residues
MRRSVIPGFGLSLGITVLYLGVIVLLPLCALILKASDIGAAQLWAILSSPRTLAAIRLTVTMALAATIFNAVYGLLLAWV
LARYQFPGKRLLDALVDVPFALPTAVAGLALTALFSKTGWFGSVLEPLGVSVVYTPLGIGIAMAFTSIPFVVRTVQPVIE
DLGSDVEEASRSLGASDLQILSKIIFPSIFPALLAGASLAFARSLGEFGAVIFIAGNQPMKTEIVALLAFIRLEEYDYPA
AATIAVVMLVMAFAMLLVTNAVQAWQLRYLGRGDR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=262, Percent_Identity=54.1984732824427, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI87082099, Length=268, Percent_Identity=35.4477611940299, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1786980, Length=224, Percent_Identity=30.3571428571429, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787369, Length=220, Percent_Identity=28.6363636363636, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786254, Length=210, Percent_Identity=23.8095238095238, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787368, Length=218, Percent_Identity=27.5229357798165, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29228; Mature: 29228

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSVIPGFGLSLGITVLYLGVIVLLPLCALILKASDIGAAQLWAILSSPRTLAAIRLTV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
TMALAATIFNAVYGLLLAWVLARYQFPGKRLLDALVDVPFALPTAVAGLALTALFSKTGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
FGSVLEPLGVSVVYTPLGIGIAMAFTSIPFVVRTVQPVIEDLGSDVEEASRSLGASDLQI
HHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
LSKIIFPSIFPALLAGASLAFARSLGEFGAVIFIAGNQPMKTEIVALLAFIRLEEYDYPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCH
AATIAVVMLVMAFAMLLVTNAVQAWQLRYLGRGDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRRSVIPGFGLSLGITVLYLGVIVLLPLCALILKASDIGAAQLWAILSSPRTLAAIRLTV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
TMALAATIFNAVYGLLLAWVLARYQFPGKRLLDALVDVPFALPTAVAGLALTALFSKTGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
FGSVLEPLGVSVVYTPLGIGIAMAFTSIPFVVRTVQPVIEDLGSDVEEASRSLGASDLQI
HHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
LSKIIFPSIFPALLAGASLAFARSLGEFGAVIFIAGNQPMKTEIVALLAFIRLEEYDYPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCH
AATIAVVMLVMAFAMLLVTNAVQAWQLRYLGRGDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]