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The map label for this gene is glcB

Identifier: 86748520

GI number: 86748520

Start: 1584818

End: 1586992

Strand: Reverse

Name: glcB

Synonym: RPB_1395

Alternate gene names: 86748520

Gene position: 1586992-1584818 (Counterclockwise)

Preceding gene: 86748521

Following gene: 86748519

Centisome position: 29.77

GC content: 66.02

Gene sequence:

>2175_bases
ATGAATCGTATCGATGCCCACGGCTTGAAAATTGCGCCCGTCCTGTTCGACTTCATCGCCAAGGAGGCCGCGCCGAAGAC
CGGCGTCGCACCGGACGCCTTCTGGGCCGGGCTCGCCGCGATCGTGCGCGATCTGACGCCGAAGACGGTCGCGCTGCTGC
AGCATCGCGACGGCCTGCAGGCCAAGATCGATGCCTGGCATCTCGCCAACAAGGGCAAGAAGCAGGACATGGCGGCCTAC
ACCGCCTTCCTGAAAGAGATCGGCTATCTGCTGCCGGAGCCGCCGACGGTCGCGGTCGAGACCGCCAATGTCGACGACGA
GATCGGCAAGCTGTGCGGCCCGCAGCTCGTGGTGCCGCTGTCGAATGCGCGCTACGCACTCAACGCGGCGAACGCGCGTT
GGGGCTCGCTGTATGACGCGTTCTATGGCACCGACGCGATCCCGCAGGAGGCCAGCCAGGCCAAGGGCTACGACAAGGCG
CGCGGCGACAAGGTGATCGCCAAGGCCAAGGCGTTCCTCGACCAGGCCGCGCCGCTGGTGGCCGGCAGCCACAGCGACGT
CACCGCCTACAGCGTGATCGCCGGCCAGCTTTCGGCAAAACTCAAGAGCGGCAACGCCACCGGCCTGAAGACCCCCCGGC
AGTTCGCCGGCTATCTGGGCGACGCCGCGTCGCCGAGCGCGGTGCTGCTGGTCAATAACGGCCTGCATATCGAGATCAAG
ATCGACCGCGCCAATACCATCGGCAAGGACGATGCGGCCGGCGTCGCCGACCTCGTGATCGAATCCGCGGTGTCGACCAT
CCTCGACATGGAGGACTCGGTCGCAGCCGTCGACGCCGAAGACAAGGTGCTGATCTACCGCAACACGCTCGGCCTGATGG
ACGGCACGCTGTCGGCCGATTTCGACAAGGGTGGCAAGACCGTCACCCGCGCGCTGAACGGCGACCGCACTTATACCGGC
CCGGACGGCAAGGACGTCACCCTGCACGGGCGCAGCCTGCTGCTGATGCGCAATGTCGGCCATCACATGTGGACCGATGC
GGTGCTCGATTCGAACGGCGACGAGATCCCCGAGGGCTTCCTCGACGCCGCGGTGTCCGGCCTACTGGCGATCCACGACC
TCAAGGCACTCGGCAAGACCCGCAACAGCCGCACCGGCTCGGTCTACATCGTCAAGCCGAAGATGCACGGCCCGGACGAA
GTGGCGCTGACTTGCGAGCTGTTCGGCCGCGTCGAGCAGATGCTCGGCCTGAAGGAGAACACGCTGAAGGTCGGCATCAT
GGACGAGGAGCGCCGCACCACGGTGAACCTCAAGGCCTGCATCCAGAACGCGTCCAAGCGCATCGTGTTCATCAACACGG
GTTTCCTGGACCGCACCGGCGACGAGATCCACACCTCGATGGAAGCGGGTCCGATGATCCGCAAGAACGAGATGAAGGCG
CAGCCCTGGATCAAGGCGTACGAGGACTGGAACGTCGACACCGGCCTGATCGACGGCCTGCCCGGCCATGCCCAGATTGG
TAAGGGCATGTGGGCGGCCCCCGACAAGATGGCCGACATGCTGACGCAGAAGATCGGCCACCCGCAGGCCGGCGCCACCA
CCGCCTGGGTGCCGTCGCCGACCGCCGCGACGCTGCACGCGCTGCACTATCACCAGGTCGACGTGCTGGCGCGTCAGCAG
GAGCTGAAGACGGGCGGCCCGCGCGCCAAGCTCGAGGACATCCTCACCATCCCGGTGTCGCAATCGAATTGGGCGCCGGA
CGACGTCCGCCAGGAGATCGACAACAACTGCCAGGGTATCCTCGGCTACGTCGTGCGCTGGATCGATCAGGGCGTCGGCT
GCTCCAAGGTGCCGGACATCCACGACGTCGGCCTGATGGAAGACCGCGCCACGCTGCGCATCTCCAGCCAGCATCTGGCG
AACTGGCTGCATCACGGCGTCGTCACCAAGGAGCAGGTGCTGGAATCGCTGAAGCGGATGGCGGCGGTGGTCGACAAGCA
GAACGCTGGTGATCCGCTGTACCGGCCGATGGCGCCGGACTTCGACGGCGTCGCCTTCGAGGCGGCGTGCGACCTGATCT
TCAAGGGCCGCGAGCAGCCGAACGGCTACACCGAATTCATCCTGCATATCCGCCGCCGCGAAGCCAAGGCGGCGCATCTG
CAGGACCTGACCTGA

Upstream 100 bases:

>100_bases
CTAAAAGAGGCACCCCCGAGGCCGCCATCCGCGGTCGTCGGCTCATTTTGACCCCTGAAACGGCCGCTGCTTTGCGCCCT
TTCTCGCGAGACATCCTCAT

Downstream 100 bases:

>100_bases
TCAGGTCAAACTTATCGTCCGCCGACAAGCAAGGCCTCTCCCGCAAGGGAGGGGTCTTTTTTACGTCCGGTAACCTGTTA
CTTGGCGCCGAGATTCGGGC

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 724; Mature: 724

Protein sequence:

>724_residues
MNRIDAHGLKIAPVLFDFIAKEAAPKTGVAPDAFWAGLAAIVRDLTPKTVALLQHRDGLQAKIDAWHLANKGKKQDMAAY
TAFLKEIGYLLPEPPTVAVETANVDDEIGKLCGPQLVVPLSNARYALNAANARWGSLYDAFYGTDAIPQEASQAKGYDKA
RGDKVIAKAKAFLDQAAPLVAGSHSDVTAYSVIAGQLSAKLKSGNATGLKTPRQFAGYLGDAASPSAVLLVNNGLHIEIK
IDRANTIGKDDAAGVADLVIESAVSTILDMEDSVAAVDAEDKVLIYRNTLGLMDGTLSADFDKGGKTVTRALNGDRTYTG
PDGKDVTLHGRSLLLMRNVGHHMWTDAVLDSNGDEIPEGFLDAAVSGLLAIHDLKALGKTRNSRTGSVYIVKPKMHGPDE
VALTCELFGRVEQMLGLKENTLKVGIMDEERRTTVNLKACIQNASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMKA
QPWIKAYEDWNVDTGLIDGLPGHAQIGKGMWAAPDKMADMLTQKIGHPQAGATTAWVPSPTAATLHALHYHQVDVLARQQ
ELKTGGPRAKLEDILTIPVSQSNWAPDDVRQEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLA
NWLHHGVVTKEQVLESLKRMAAVVDKQNAGDPLYRPMAPDFDGVAFEAACDLIFKGREQPNGYTEFILHIRRREAKAAHL
QDLT

Sequences:

>Translated_724_residues
MNRIDAHGLKIAPVLFDFIAKEAAPKTGVAPDAFWAGLAAIVRDLTPKTVALLQHRDGLQAKIDAWHLANKGKKQDMAAY
TAFLKEIGYLLPEPPTVAVETANVDDEIGKLCGPQLVVPLSNARYALNAANARWGSLYDAFYGTDAIPQEASQAKGYDKA
RGDKVIAKAKAFLDQAAPLVAGSHSDVTAYSVIAGQLSAKLKSGNATGLKTPRQFAGYLGDAASPSAVLLVNNGLHIEIK
IDRANTIGKDDAAGVADLVIESAVSTILDMEDSVAAVDAEDKVLIYRNTLGLMDGTLSADFDKGGKTVTRALNGDRTYTG
PDGKDVTLHGRSLLLMRNVGHHMWTDAVLDSNGDEIPEGFLDAAVSGLLAIHDLKALGKTRNSRTGSVYIVKPKMHGPDE
VALTCELFGRVEQMLGLKENTLKVGIMDEERRTTVNLKACIQNASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMKA
QPWIKAYEDWNVDTGLIDGLPGHAQIGKGMWAAPDKMADMLTQKIGHPQAGATTAWVPSPTAATLHALHYHQVDVLARQQ
ELKTGGPRAKLEDILTIPVSQSNWAPDDVRQEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLA
NWLHHGVVTKEQVLESLKRMAAVVDKQNAGDPLYRPMAPDFDGVAFEAACDLIFKGREQPNGYTEFILHIRRREAKAAHL
QDLT
>Mature_724_residues
MNRIDAHGLKIAPVLFDFIAKEAAPKTGVAPDAFWAGLAAIVRDLTPKTVALLQHRDGLQAKIDAWHLANKGKKQDMAAY
TAFLKEIGYLLPEPPTVAVETANVDDEIGKLCGPQLVVPLSNARYALNAANARWGSLYDAFYGTDAIPQEASQAKGYDKA
RGDKVIAKAKAFLDQAAPLVAGSHSDVTAYSVIAGQLSAKLKSGNATGLKTPRQFAGYLGDAASPSAVLLVNNGLHIEIK
IDRANTIGKDDAAGVADLVIESAVSTILDMEDSVAAVDAEDKVLIYRNTLGLMDGTLSADFDKGGKTVTRALNGDRTYTG
PDGKDVTLHGRSLLLMRNVGHHMWTDAVLDSNGDEIPEGFLDAAVSGLLAIHDLKALGKTRNSRTGSVYIVKPKMHGPDE
VALTCELFGRVEQMLGLKENTLKVGIMDEERRTTVNLKACIQNASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMKA
QPWIKAYEDWNVDTGLIDGLPGHAQIGKGMWAAPDKMADMLTQKIGHPQAGATTAWVPSPTAATLHALHYHQVDVLARQQ
ELKTGGPRAKLEDILTIPVSQSNWAPDDVRQEIDNNCQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLA
NWLHHGVVTKEQVLESLKRMAAVVDKQNAGDPLYRPMAPDFDGVAFEAACDLIFKGREQPNGYTEFILHIRRREAKAAHL
QDLT

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily

Homologues:

Organism=Escherichia coli, GI1789348, Length=717, Percent_Identity=59.9721059972106, Blast_Score=890, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MASZ_RHOP2 (Q2J0A5)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485016.1
- ProteinModelPortal:   Q2J0A5
- SMR:   Q2J0A5
- STRING:   Q2J0A5
- GeneID:   3908345
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1395
- eggNOG:   COG2225
- HOGENOM:   HBG350005
- OMA:   PKMHGPD
- ProtClustDB:   PRK02999
- BioCyc:   RPAL316058:RPB_1395-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00641
- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253
- PANTHER:   PTHR21631:SF1
- TIGRFAMs:   TIGR01345

Pfam domain/function: PF01274 Malate_synthase; SSF51645 Malat_synth_like

EC number: =2.3.3.9

Molecular weight: Translated: 78444; Mature: 78444

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00589 PTS_HPR_SER

Important sites: ACT_SITE 337-337 ACT_SITE 628-628

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDAHGLKIAPVLFDFIAKEAAPKTGVAPDAFWAGLAAIVRDLTPKTVALLQHRDGLQ
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
AKIDAWHLANKGKKQDMAAYTAFLKEIGYLLPEPPTVAVETANVDDEIGKLCGPQLVVPL
EEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCEEEEEE
SNARYALNAANARWGSLYDAFYGTDAIPQEASQAKGYDKARGDKVIAKAKAFLDQAAPLV
CCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCE
AGSHSDVTAYSVIAGQLSAKLKSGNATGLKTPRQFAGYLGDAASPSAVLLVNNGLHIEIK
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEE
IDRANTIGKDDAAGVADLVIESAVSTILDMEDSVAAVDAEDKVLIYRNTLGLMDGTLSAD
EECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCCCC
FDKGGKTVTRALNGDRTYTGPDGKDVTLHGRSLLLMRNVGHHMWTDAVLDSNGDEIPEGF
CCCCCHHHHHHHCCCCEECCCCCCEEEEECCEEEEEHHCCHHHHHHHHCCCCCCCCHHHH
LDAAVSGLLAIHDLKALGKTRNSRTGSVYIVKPKMHGPDEVALTCELFGRVEQMLGLKEN
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCC
TLKVGIMDEERRTTVNLKACIQNASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMKA
CEEEEEECCCCHHEEHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCCC
QPWIKAYEDWNVDTGLIDGLPGHAQIGKGMWAAPDKMADMLTQKIGHPQAGATTAWVPSP
CCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCC
TAATLHALHYHQVDVLARQQELKTGGPRAKLEDILTIPVSQSNWAPDDVRQEIDNNCQGI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHH
LGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLANWLHHGVVTKEQVLESLKRM
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHH
AAVVDKQNAGDPLYRPMAPDFDGVAFEAACDLIFKGREQPNGYTEFILHIRRREAKAAHL
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
QDLT
CCCC
>Mature Secondary Structure
MNRIDAHGLKIAPVLFDFIAKEAAPKTGVAPDAFWAGLAAIVRDLTPKTVALLQHRDGLQ
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
AKIDAWHLANKGKKQDMAAYTAFLKEIGYLLPEPPTVAVETANVDDEIGKLCGPQLVVPL
EEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCEEEEEE
SNARYALNAANARWGSLYDAFYGTDAIPQEASQAKGYDKARGDKVIAKAKAFLDQAAPLV
CCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCE
AGSHSDVTAYSVIAGQLSAKLKSGNATGLKTPRQFAGYLGDAASPSAVLLVNNGLHIEIK
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEE
IDRANTIGKDDAAGVADLVIESAVSTILDMEDSVAAVDAEDKVLIYRNTLGLMDGTLSAD
EECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCCCC
FDKGGKTVTRALNGDRTYTGPDGKDVTLHGRSLLLMRNVGHHMWTDAVLDSNGDEIPEGF
CCCCCHHHHHHHCCCCEECCCCCCEEEEECCEEEEEHHCCHHHHHHHHCCCCCCCCHHHH
LDAAVSGLLAIHDLKALGKTRNSRTGSVYIVKPKMHGPDEVALTCELFGRVEQMLGLKEN
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCCC
TLKVGIMDEERRTTVNLKACIQNASKRIVFINTGFLDRTGDEIHTSMEAGPMIRKNEMKA
CEEEEEECCCCHHEEHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEECCCCCC
QPWIKAYEDWNVDTGLIDGLPGHAQIGKGMWAAPDKMADMLTQKIGHPQAGATTAWVPSP
CCCHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCC
TAATLHALHYHQVDVLARQQELKTGGPRAKLEDILTIPVSQSNWAPDDVRQEIDNNCQGI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHH
LGYVVRWIDQGVGCSKVPDIHDVGLMEDRATLRISSQHLANWLHHGVVTKEQVLESLKRM
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHH
AAVVDKQNAGDPLYRPMAPDFDGVAFEAACDLIFKGREQPNGYTEFILHIRRREAKAAHL
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
QDLT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA