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The map label for this gene is menB [C]

Identifier: 86748365

GI number: 86748365

Start: 1419324

End: 1420172

Strand: Reverse

Name: menB [C]

Synonym: RPB_1240

Alternate gene names: 86748365

Gene position: 1420172-1419324 (Counterclockwise)

Preceding gene: 86748369

Following gene: 86748364

Centisome position: 26.64

GC content: 65.14

Gene sequence:

>849_bases
ATGGGCGGCTTCGAGACCCTCGTGGTCGAACGTCCCGATCCGGCGATCGCGCGGATCGTGATGAACCGGCCGGAAGCGCG
CAACGCGCAGAACCTGCAGATGACCTACGACCTCAACGCCGCGTTCGACGACGCGGTGCAGGACGATGCCGTGAAGGTCA
TCATTCTCGCCGGCTCCGGCCCGCATTTCTCCGCCGGTCACGACCTGCGCGCCACCACCAAGAACGAAGCCGGCATCGAC
TTCCCGCCGGTCGGCCATTGGGGCGGCTTCCGCGAGCCCGGCGCTCACGGACGGATGGCGCGCGAGCAGGAAATTTATCT
GCAGATCACGCGGCGCTGGCGCAATCTCGCCAAGCCGACCATCGCCGAAGTGCACGGCAAGTGCATCGCCGGCGGGCTGA
TGCTGGCCTGGGCCTGCGACCTGATCGTCGCCGCCGACAATGCCGAGTTCTGCGACCCGGTGGTGACGATGGGCGTCTGC
GGCGTCGAGTGGTTCGTGCATCCATGGGAGCTCGGCCCGCGCAAGGCCAAGGAACTGCTGTTCACCGCCGATAGCTGGAG
TGCGCAAGACGCGCACCGGCTCGGCATGGTCAATCATGTGGTGGCCGCCGCCGAGCTATCCGCCTTCACCTTGAATCTGG
CGCAGCGCATCGCCGCCAAGCCGAGCTTCGCGCTGAAGATGACCAAGGAAGCGGTCAACCGCTCGGTGGACATTCAGGGT
CAGCCGGCCGCGATCGATCAGGCGTTCGCGCTGCATCAGCTTTGTCACGCACACAATCTGCAGGAGTTTGGAATGATCGT
CGATCCGTCGGGACTGCATCCGTCGGTGCGCAAGCAAGTGAAGGTGTAA

Upstream 100 bases:

>100_bases
GCCGCTGCAGATGACGTTTGCGGTCGCCGCAGCGACCTGCCAGACTACGCGCAACCACAACGGCGCGCATAAGCCGAAAC
AACAAGAAAAGGGAGGATGC

Downstream 100 bases:

>100_bases
GGCGATGGATCTCACGCCCAGTGACGAACAGCGGCTGCTGCGCGAAAGCGCCGACCGCTTCATCAACGAGACCTACACCA
GCAAGCTGCGCGAGCAGAAC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGGFETLVVERPDPAIARIVMNRPEARNAQNLQMTYDLNAAFDDAVQDDAVKVIILAGSGPHFSAGHDLRATTKNEAGID
FPPVGHWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPTIAEVHGKCIAGGLMLAWACDLIVAADNAEFCDPVVTMGVC
GVEWFVHPWELGPRKAKELLFTADSWSAQDAHRLGMVNHVVAAAELSAFTLNLAQRIAAKPSFALKMTKEAVNRSVDIQG
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV

Sequences:

>Translated_282_residues
MGGFETLVVERPDPAIARIVMNRPEARNAQNLQMTYDLNAAFDDAVQDDAVKVIILAGSGPHFSAGHDLRATTKNEAGID
FPPVGHWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPTIAEVHGKCIAGGLMLAWACDLIVAADNAEFCDPVVTMGVC
GVEWFVHPWELGPRKAKELLFTADSWSAQDAHRLGMVNHVVAAAELSAFTLNLAQRIAAKPSFALKMTKEAVNRSVDIQG
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV
>Mature_281_residues
GGFETLVVERPDPAIARIVMNRPEARNAQNLQMTYDLNAAFDDAVQDDAVKVIILAGSGPHFSAGHDLRATTKNEAGIDF
PPVGHWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPTIAEVHGKCIAGGLMLAWACDLIVAADNAEFCDPVVTMGVCG
VEWFVHPWELGPRKAKELLFTADSWSAQDAHRLGMVNHVVAAAELSAFTLNLAQRIAAKPSFALKMTKEAVNRSVDIQGQ
PAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=205, Percent_Identity=33.6585365853659, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI45643119, Length=234, Percent_Identity=29.0598290598291, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI260275230, Length=234, Percent_Identity=29.0598290598291, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI260274832, Length=234, Percent_Identity=29.0598290598291, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=28.1632653061224, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788597, Length=241, Percent_Identity=31.9502074688797, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI221142681, Length=222, Percent_Identity=35.1351351351351, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=27.0916334661355, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787660, Length=230, Percent_Identity=29.5652173913043, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87082183, Length=220, Percent_Identity=31.8181818181818, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=230, Percent_Identity=27.8260869565217, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=258, Percent_Identity=28.6821705426357, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=226, Percent_Identity=28.7610619469027, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=218, Percent_Identity=25.6880733944954, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=236, Percent_Identity=26.6949152542373, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=204, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=237, Percent_Identity=24.4725738396624, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=24.4897959183673, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=26.7543859649123, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20129971, Length=229, Percent_Identity=27.9475982532751, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653477, Length=229, Percent_Identity=27.9475982532751, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920382, Length=248, Percent_Identity=29.4354838709677, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357171, Length=244, Percent_Identity=28.6885245901639, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=247, Percent_Identity=29.1497975708502, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30825; Mature: 30693

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGFETLVVERPDPAIARIVMNRPEARNAQNLQMTYDLNAAFDDAVQDDAVKVIILAGSG
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCCCEEEEEEECCC
PHFSAGHDLRATTKNEAGIDFPPVGHWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPT
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
IAEVHGKCIAGGLMLAWACDLIVAADNAEFCDPVVTMGVCGVEWFVHPWELGPRKAKELL
HHHHCCHHHHHHHHHHHHHCEEEECCCCHHHCHHHHHHHHHHHHEECCCCCCHHHHHHEE
FTADSWSAQDAHRLGMVNHVVAAAELSAFTLNLAQRIAAKPSFALKMTKEAVNRSVDIQG
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCC
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV
CCHHHHHHHHHHHHHHHCCHHHCCEEECCCCCCHHHHHHCCC
>Mature Secondary Structure 
GGFETLVVERPDPAIARIVMNRPEARNAQNLQMTYDLNAAFDDAVQDDAVKVIILAGSG
CCCEEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCCCEEEEEEECCC
PHFSAGHDLRATTKNEAGIDFPPVGHWGGFREPGAHGRMAREQEIYLQITRRWRNLAKPT
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
IAEVHGKCIAGGLMLAWACDLIVAADNAEFCDPVVTMGVCGVEWFVHPWELGPRKAKELL
HHHHCCHHHHHHHHHHHHHCEEEECCCCHHHCHHHHHHHHHHHHEECCCCCCHHHHHHEE
FTADSWSAQDAHRLGMVNHVVAAAELSAFTLNLAQRIAAKPSFALKMTKEAVNRSVDIQG
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCC
QPAAIDQAFALHQLCHAHNLQEFGMIVDPSGLHPSVRKQVKV
CCHHHHHHHHHHHHHHHCCHHHCCEEECCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA