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The map label for this gene is lcfB [H]

Identifier: 86748369

GI number: 86748369

Start: 1422634

End: 1424142

Strand: Reverse

Name: lcfB [H]

Synonym: RPB_1244

Alternate gene names: 86748369

Gene position: 1424142-1422634 (Counterclockwise)

Preceding gene: 86748375

Following gene: 86748365

Centisome position: 26.71

GC content: 67.86

Gene sequence:

>1509_bases
ATGGCGCTTCACGACTTCACCCTGGGCGATGTCTATCGCCGCAATGCATCGCTGTTTCCGGACCGCACGGCTTTCGTGAT
CGATGGCGTCCGCGTCACCCACCGCGACTATCTCGCCCGGATCGAGCGGCTGGCCGCCGGCCTGCTGCGCGACAACGTCC
GGCCCGGCGACCGTGTTGCGATCCTGTCGCAGAACTGTCCGGAAATGGTCGAGCTGATCGGCGCGGTAGCGTTGATCGGC
GCCATCCTGCTGCCGGTGAACTATCGGCTCAACGCCGAGGAGATCGGCTTCGTGCTCGGCGACGGCGCGCCGACCGTGCT
GATCGCGGGCAGTGATTACCGCGACATCATCGCCGGGCTGCAGCCGTCGCTGGCGAGCGTGCGAAAGACCTATGCGATCG
GCGCCGATCCCGGCCCGTTCGCGCCGTTCGCCGAGCTCGAGTCCGACGATGCCTTCGTGCCACCGGAGGTCGCCGCCGCC
GACGGCTTCGTCATCATCCACACCGCCGCCGTCGGCGGCAAACCGCGCGGCGCGCTGATCTCGCAGGGCAATCTGTTGAT
CGCGCAGAGTTCGCTGATCGAGGCATGGCGGCTGACCGAGCGCGACGTCAATCTCGGCATGCTGCCGTTGTTTCACGTCA
CCGGCCTCGGCCTGATGCTGACGCTGCAGCAGGCCGGCGGCGCCAGCGTGATTTCGGCGAAGTTCGATCCGGCGCAGGCG
GCGCGCGACATCGAGGCGCACCGCGTCACGGTGCTGGCCGAGTTCGCGCCGACGCTCGGCAATATTCTCGATCAGGCTCA
GTCGGCGCAATTGCAGAGCCTGCGCGCCGTCACCGGCCTCGATACGCCGGAGACGATCGAGCGGTTCGAGAAAGAGTGTC
CGCAGGCGACGTTCTGGGCGACCTTCGGCCAGTCCGAGACTTCGGGGCTGGCGACGTTTGCGCCGTATCGCGACCGGCCG
AAATCCGCCGGGCGACCGTTGCCCTGGCGCACGATCGCGGTGGTCGATGCCGACGATCGCCCGCTGCCCGCGGGTGAGGT
CGGCGAGATCGTGCTGCGCGGCCCGACCGTGTTCAAAGGCTACTGGAACAACGAGGCGGCGACCCAGCACGCCTTCCGCA
ACGGCTGGCACCACACCGGCGACATGGGGAAGTTCGACGCCGACGGCTATCTGTTCTACGCCGGCCGCGCGCCGGAAAAG
GAACTGATCAAGACCGGCGGCGAGAACGTCTATCCGGCCGAGGTCGAAGGCGCGCTGAAGCAGCATCCGGCGATCGCCGA
GGCCGTGGTGATCGGCGTGCCCGATCCGCAATGGAGCGAGGCCATCAAGGCGGTGTGCGTCTGCAAGCCCGGTCAAAACG
TCGACGCCGTGACGCTGGCCGACTTTGTCGGCTCGCTGATCGCGCGCTACAAGAAGCCGAAGCACGTGGTGTTCGTCGAC
GCCTTGCCCAAGGACGCCAAGGGCGCGGTCGACCGCGCCGCGGTGAAGGCCGCGCACGGAGGGGCGTAA

Upstream 100 bases:

>100_bases
TTTCGCTAAAAGGCTGACGGCCCGCCCGCCGACCCGGCGCAGGGGCAAAGCAATCGGGCCGGCCGGACGCGGCTGAAGCC
TTGAAAGACGGGGGAAATGC

Downstream 100 bases:

>100_bases
CGTCAACGCAGCGAGAAAATCACCGGCACGGTGAAGCTGGCCGAGGACTGCGTCATCTCCGGCGGGAATGCCGGTAGCGG
CTGGGCGCGGCGGATCATCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MALHDFTLGDVYRRNASLFPDRTAFVIDGVRVTHRDYLARIERLAAGLLRDNVRPGDRVAILSQNCPEMVELIGAVALIG
AILLPVNYRLNAEEIGFVLGDGAPTVLIAGSDYRDIIAGLQPSLASVRKTYAIGADPGPFAPFAELESDDAFVPPEVAAA
DGFVIIHTAAVGGKPRGALISQGNLLIAQSSLIEAWRLTERDVNLGMLPLFHVTGLGLMLTLQQAGGASVISAKFDPAQA
ARDIEAHRVTVLAEFAPTLGNILDQAQSAQLQSLRAVTGLDTPETIERFEKECPQATFWATFGQSETSGLATFAPYRDRP
KSAGRPLPWRTIAVVDADDRPLPAGEVGEIVLRGPTVFKGYWNNEAATQHAFRNGWHHTGDMGKFDADGYLFYAGRAPEK
ELIKTGGENVYPAEVEGALKQHPAIAEAVVIGVPDPQWSEAIKAVCVCKPGQNVDAVTLADFVGSLIARYKKPKHVVFVD
ALPKDAKGAVDRAAVKAAHGGA

Sequences:

>Translated_502_residues
MALHDFTLGDVYRRNASLFPDRTAFVIDGVRVTHRDYLARIERLAAGLLRDNVRPGDRVAILSQNCPEMVELIGAVALIG
AILLPVNYRLNAEEIGFVLGDGAPTVLIAGSDYRDIIAGLQPSLASVRKTYAIGADPGPFAPFAELESDDAFVPPEVAAA
DGFVIIHTAAVGGKPRGALISQGNLLIAQSSLIEAWRLTERDVNLGMLPLFHVTGLGLMLTLQQAGGASVISAKFDPAQA
ARDIEAHRVTVLAEFAPTLGNILDQAQSAQLQSLRAVTGLDTPETIERFEKECPQATFWATFGQSETSGLATFAPYRDRP
KSAGRPLPWRTIAVVDADDRPLPAGEVGEIVLRGPTVFKGYWNNEAATQHAFRNGWHHTGDMGKFDADGYLFYAGRAPEK
ELIKTGGENVYPAEVEGALKQHPAIAEAVVIGVPDPQWSEAIKAVCVCKPGQNVDAVTLADFVGSLIARYKKPKHVVFVD
ALPKDAKGAVDRAAVKAAHGGA
>Mature_501_residues
ALHDFTLGDVYRRNASLFPDRTAFVIDGVRVTHRDYLARIERLAAGLLRDNVRPGDRVAILSQNCPEMVELIGAVALIGA
ILLPVNYRLNAEEIGFVLGDGAPTVLIAGSDYRDIIAGLQPSLASVRKTYAIGADPGPFAPFAELESDDAFVPPEVAAAD
GFVIIHTAAVGGKPRGALISQGNLLIAQSSLIEAWRLTERDVNLGMLPLFHVTGLGLMLTLQQAGGASVISAKFDPAQAA
RDIEAHRVTVLAEFAPTLGNILDQAQSAQLQSLRAVTGLDTPETIERFEKECPQATFWATFGQSETSGLATFAPYRDRPK
SAGRPLPWRTIAVVDADDRPLPAGEVGEIVLRGPTVFKGYWNNEAATQHAFRNGWHHTGDMGKFDADGYLFYAGRAPEKE
LIKTGGENVYPAEVEGALKQHPAIAEAVVIGVPDPQWSEAIKAVCVCKPGQNVDAVTLADFVGSLIARYKKPKHVVFVDA
LPKDAKGAVDRAAVKAAHGGA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=27.8195488721804, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=27.8195488721804, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI156151445, Length=545, Percent_Identity=25.3211009174312, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI42544132, Length=490, Percent_Identity=23.8775510204082, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI38505220, Length=490, Percent_Identity=25.9183673469388, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI122937307, Length=494, Percent_Identity=25.9109311740891, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI58082049, Length=477, Percent_Identity=26.4150943396226, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI115511026, Length=506, Percent_Identity=26.2845849802372, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI157311624, Length=498, Percent_Identity=26.5060240963855, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI157311622, Length=498, Percent_Identity=26.5060240963855, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI27477105, Length=157, Percent_Identity=41.4012738853503, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI13375727, Length=544, Percent_Identity=21.5073529411765, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI40807491, Length=468, Percent_Identity=23.2905982905983, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI57165412, Length=152, Percent_Identity=35.5263157894737, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI57165410, Length=152, Percent_Identity=35.5263157894737, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=28.7378640776699, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=25.6120527306968, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI145693145, Length=523, Percent_Identity=27.151051625239, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1786810, Length=521, Percent_Identity=26.679462571977, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=25.4646840148699, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788595, Length=489, Percent_Identity=24.3353783231084, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=502, Percent_Identity=29.8804780876494, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71994703, Length=546, Percent_Identity=24.5421245421245, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=546, Percent_Identity=24.5421245421245, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=546, Percent_Identity=24.5421245421245, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=494, Percent_Identity=26.3157894736842, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17557194, Length=535, Percent_Identity=26.3551401869159, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=444, Percent_Identity=25, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=454, Percent_Identity=27.3127753303965, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=550, Percent_Identity=25.6363636363636, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=521, Percent_Identity=24.3761996161228, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=514, Percent_Identity=24.5136186770428, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=514, Percent_Identity=23.5408560311284, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=205, Percent_Identity=30.7317073170732, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=507, Percent_Identity=28.5996055226824, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319264, Length=551, Percent_Identity=23.049001814882, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323182, Length=555, Percent_Identity=23.4234234234234, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859661, Length=473, Percent_Identity=28.1183932346723, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355181, Length=513, Percent_Identity=26.9005847953216, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24581924, Length=540, Percent_Identity=24.4444444444444, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24648253, Length=517, Percent_Identity=24.1779497098646, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161076582, Length=515, Percent_Identity=25.631067961165, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24648255, Length=517, Percent_Identity=24.1779497098646, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24653035, Length=512, Percent_Identity=24.8046875, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922652, Length=524, Percent_Identity=23.8549618320611, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20130357, Length=508, Percent_Identity=23.6220472440945, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24656500, Length=519, Percent_Identity=26.3969171483622, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356947, Length=502, Percent_Identity=23.9043824701195, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358303, Length=529, Percent_Identity=23.4404536862004, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648257, Length=518, Percent_Identity=25.2895752895753, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281365686, Length=345, Percent_Identity=26.0869565217391, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356441, Length=458, Percent_Identity=23.7991266375546, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648260, Length=485, Percent_Identity=22.8865979381443, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19921316, Length=543, Percent_Identity=23.7569060773481, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=23.8721804511278, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667955, Length=532, Percent_Identity=23.8721804511278, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667959, Length=385, Percent_Identity=25.1948051948052, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17933690, Length=134, Percent_Identity=34.3283582089552, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 53690; Mature: 53558

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHDFTLGDVYRRNASLFPDRTAFVIDGVRVTHRDYLARIERLAAGLLRDNVRPGDRVA
CCCCCCCHHHHHHCCCCCCCCCCEEEEECEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ILSQNCPEMVELIGAVALIGAILLPVNYRLNAEEIGFVLGDGAPTVLIAGSDYRDIIAGL
EECCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHCCEEEECCCCEEEEECCCHHHHHHCC
QPSLASVRKTYAIGADPGPFAPFAELESDDAFVPPEVAAADGFVIIHTAAVGGKPRGALI
CHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCEE
SQGNLLIAQSSLIEAWRLTERDVNLGMLPLFHVTGLGLMLTLQQAGGASVISAKFDPAQA
ECCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCEEEEECCCHHHH
ARDIEAHRVTVLAEFAPTLGNILDQAQSAQLQSLRAVTGLDTPETIERFEKECPQATFWA
HHHCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEE
TFGQSETSGLATFAPYRDRPKSAGRPLPWRTIAVVDADDRPLPAGEVGEIVLRGPTVFKG
ECCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCEEEEE
YWNNEAATQHAFRNGWHHTGDMGKFDADGYLFYAGRAPEKELIKTGGENVYPAEVEGALK
ECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHH
QHPAIAEAVVIGVPDPQWSEAIKAVCVCKPGQNVDAVTLADFVGSLIARYKKPKHVVFVD
HCCCCEEEEEEECCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
ALPKDAKGAVDRAAVKAAHGGA
CCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALHDFTLGDVYRRNASLFPDRTAFVIDGVRVTHRDYLARIERLAAGLLRDNVRPGDRVA
CCCCCCHHHHHHCCCCCCCCCCEEEEECEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ILSQNCPEMVELIGAVALIGAILLPVNYRLNAEEIGFVLGDGAPTVLIAGSDYRDIIAGL
EECCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHCCEEEECCCCEEEEECCCHHHHHHCC
QPSLASVRKTYAIGADPGPFAPFAELESDDAFVPPEVAAADGFVIIHTAAVGGKPRGALI
CHHHHHHHHHEEECCCCCCCCCHHHCCCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCEE
SQGNLLIAQSSLIEAWRLTERDVNLGMLPLFHVTGLGLMLTLQQAGGASVISAKFDPAQA
ECCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCCCCEEEEECCCHHHH
ARDIEAHRVTVLAEFAPTLGNILDQAQSAQLQSLRAVTGLDTPETIERFEKECPQATFWA
HHHCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEE
TFGQSETSGLATFAPYRDRPKSAGRPLPWRTIAVVDADDRPLPAGEVGEIVLRGPTVFKG
ECCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCEEEEE
YWNNEAATQHAFRNGWHHTGDMGKFDADGYLFYAGRAPEKELIKTGGENVYPAEVEGALK
ECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHH
QHPAIAEAVVIGVPDPQWSEAIKAVCVCKPGQNVDAVTLADFVGSLIARYKKPKHVVFVD
HCCCCEEEEEEECCCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
ALPKDAKGAVDRAAVKAAHGGA
CCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]