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The map label for this gene is proB

Identifier: 86747378

GI number: 86747378

Start: 285115

End: 286245

Strand: Direct

Name: proB

Synonym: RPB_0252

Alternate gene names: 86747378

Gene position: 285115-286245 (Clockwise)

Preceding gene: 86747377

Following gene: 86747379

Centisome position: 5.35

GC content: 70.56

Gene sequence:

>1131_bases
ATGGCCCGCCCGAAGCTCCATGATTTCCGCCGCATCGTCGTCAAGGTCGGCTCCTCGCTGCTGATCGATTCCGGCGCCGG
CGAGGTCCGGGCGACATGGCTCGCCGCGCTCGCCGCCGACATCGCCGAGCTGCATCGCGACGGCCGCGACGTGATGATCG
TCTCCTCCGGCTCGATCGCGCTCGGCCGCAGCCGACTGAAGCTGCCGCGCGGCGCGCTGAAGCTCGAGGAAAGCCAGGCC
GCCGCGGCCGTCGGCCAGATCGCGCTGGCGCGGACCTGGTCGGAAGTGCTGGGCCACCATGGGATCGGCGCTGGGCAGAT
CCTGGTGACGCTGCAGGACACCGAGGAGCGGCGGCGCTATCTCAACGCCCGCTCGACCATCGCCAAGCTGCTGGAATGGC
GCGCGGTACCGGTGATCAACGAGAACGACACCGTCGCCACCAACGAAATCCGCTACGGCGACAACGACCGCCTCGCCGCG
CGGGTCGCGACCATGGCGAGTGCGGATCTCCTGATCCTGCTGTCCGACATCGACGGCCTCTACACCGCCCCGCCCGGCAG
CAATCCGGATGCGAAGCTGATCCCGGTGGTCGACTCCGTCACCGCCGAAATCGAGGGCATGGCCGGCGCGGCCGGCTCGG
AGCTGTCGCGCGGCGGGATGCGCACCAAGATCGAGGCCGCCAAGATCGCGACCTCGGCCGGCACCCACATGCTGATCGCT
TCGGGCAAGATCGACCATCCGCTCAAGGCCATTGCCGGCGGCGGCCCCTGCACCTGGTTCCTGACACCCGCGAACCCGGT
GACGGCTCGCAAACGCTGGATCGCGGGTTCTCTGGAGCCGAAGGGCACGCTGACGATCGACGCCGGCGCGGTGACGGCGC
TGCGCGCCGGCAAGAGCCTGCTGCCGGCCGGCGTGATCCGGGTCGACGGCCAGTTCGCCCGCGGCGACGCGGTGATCGTG
CGCGGCCCGGACACCCACGAAATCGGCCGCGGCTTGGTCGCCTACGACGCCGACGACGCCGACAGGATCAAGGGCCGCTC
CTCGCCCGACGTGATGAGCATCCTCGGCGTCAGCGGCCGCGCCGAGATGATCCACCGCGACGATCTGGTGGTGGGAACGG
CGCCGGGCTAG

Upstream 100 bases:

>100_bases
CCGGCGCGCTTGCACCCGCCGGTTCGGTGCGGCAAAACCAACCCGCCCGTCGACGGCGCGCCGCCGATGCGTCCCGTCCT
CGTCTCGCCCGATAGTCCCG

Downstream 100 bases:

>100_bases
TCTCGGGCCGCGAATTCCGGGCTGCTGTCGGCGAGGCGCCGCCTGCTGTCAACGCGCGAGTATTCGCCCGGTTTCTCCGC
AACCAGCTTGGCGATCCGGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MARPKLHDFRRIVVKVGSSLLIDSGAGEVRATWLAALAADIAELHRDGRDVMIVSSGSIALGRSRLKLPRGALKLEESQA
AAAVGQIALARTWSEVLGHHGIGAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAA
RVATMASADLLILLSDIDGLYTAPPGSNPDAKLIPVVDSVTAEIEGMAGAAGSELSRGGMRTKIEAAKIATSAGTHMLIA
SGKIDHPLKAIAGGGPCTWFLTPANPVTARKRWIAGSLEPKGTLTIDAGAVTALRAGKSLLPAGVIRVDGQFARGDAVIV
RGPDTHEIGRGLVAYDADDADRIKGRSSPDVMSILGVSGRAEMIHRDDLVVGTAPG

Sequences:

>Translated_376_residues
MARPKLHDFRRIVVKVGSSLLIDSGAGEVRATWLAALAADIAELHRDGRDVMIVSSGSIALGRSRLKLPRGALKLEESQA
AAAVGQIALARTWSEVLGHHGIGAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAA
RVATMASADLLILLSDIDGLYTAPPGSNPDAKLIPVVDSVTAEIEGMAGAAGSELSRGGMRTKIEAAKIATSAGTHMLIA
SGKIDHPLKAIAGGGPCTWFLTPANPVTARKRWIAGSLEPKGTLTIDAGAVTALRAGKSLLPAGVIRVDGQFARGDAVIV
RGPDTHEIGRGLVAYDADDADRIKGRSSPDVMSILGVSGRAEMIHRDDLVVGTAPG
>Mature_375_residues
ARPKLHDFRRIVVKVGSSLLIDSGAGEVRATWLAALAADIAELHRDGRDVMIVSSGSIALGRSRLKLPRGALKLEESQAA
AAVGQIALARTWSEVLGHHGIGAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAAR
VATMASADLLILLSDIDGLYTAPPGSNPDAKLIPVVDSVTAEIEGMAGAAGSELSRGGMRTKIEAAKIATSAGTHMLIAS
GKIDHPLKAIAGGGPCTWFLTPANPVTARKRWIAGSLEPKGTLTIDAGAVTALRAGKSLLPAGVIRVDGQFARGDAVIVR
GPDTHEIGRGLVAYDADDADRIKGRSSPDVMSILGVSGRAEMIHRDDLVVGTAPG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=323, Percent_Identity=31.8885448916409, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI62912457, Length=321, Percent_Identity=31.7757009345794, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786437, Length=370, Percent_Identity=41.0810810810811, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17569967, Length=276, Percent_Identity=31.8840579710145, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=276, Percent_Identity=31.8840579710145, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=412, Percent_Identity=33.7378640776699, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=411, Percent_Identity=32.360097323601, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI21357643, Length=293, Percent_Identity=31.740614334471, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_RHOP2 (Q2J3J7)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483874.1
- ProteinModelPortal:   Q2J3J7
- SMR:   Q2J3J7
- STRING:   Q2J3J7
- GeneID:   3907880
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0252
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   RPAL316058:RPB_0252-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39476; Mature: 39345

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPKLHDFRRIVVKVGSSLLIDSGAGEVRATWLAALAADIAELHRDGRDVMIVSSGSIA
CCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEE
LGRSRLKLPRGALKLEESQAAAAVGQIALARTWSEVLGHHGIGAGQILVTLQDTEERRRY
ECHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHH
LNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAARVATMASADLLILLSDIDGL
HHHHHHHHHHHHHCCCEEECCCCCEEECEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
YTAPPGSNPDAKLIPVVDSVTAEIEGMAGAAGSELSRGGMRTKIEAAKIATSAGTHMLIA
EECCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCCEEEHHHHHECCCCCEEEEE
SGKIDHPLKAIAGGGPCTWFLTPANPVTARKRWIAGSLEPKGTLTIDAGAVTALRAGKSL
CCCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHEECCCCCCCEEEECCCCHHHHHCCCCC
LPAGVIRVDGQFARGDAVIVRGPDTHEIGRGLVAYDADDADRIKGRSSPDVMSILGVSGR
CCCCEEEECCEEECCCEEEEECCCCHHHCCCEEEECCCCHHHHCCCCCCCHHHHHCCCCC
AEMIHRDDLVVGTAPG
HHEEECCCEEEECCCC
>Mature Secondary Structure 
ARPKLHDFRRIVVKVGSSLLIDSGAGEVRATWLAALAADIAELHRDGRDVMIVSSGSIA
CCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEE
LGRSRLKLPRGALKLEESQAAAAVGQIALARTWSEVLGHHGIGAGQILVTLQDTEERRRY
ECHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHH
LNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAARVATMASADLLILLSDIDGL
HHHHHHHHHHHHHCCCEEECCCCCEEECEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
YTAPPGSNPDAKLIPVVDSVTAEIEGMAGAAGSELSRGGMRTKIEAAKIATSAGTHMLIA
EECCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCCEEEHHHHHECCCCCEEEEE
SGKIDHPLKAIAGGGPCTWFLTPANPVTARKRWIAGSLEPKGTLTIDAGAVTALRAGKSL
CCCCCCCHHHHCCCCCEEEEECCCCCHHHHHHHEECCCCCCCEEEECCCCHHHHHCCCCC
LPAGVIRVDGQFARGDAVIVRGPDTHEIGRGLVAYDADDADRIKGRSSPDVMSILGVSGR
CCCCEEEECCEEECCCEEEEECCCCHHHCCCEEEECCCCHHHHCCCCCCCHHHHHCCCCC
AEMIHRDDLVVGTAPG
HHEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA