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The map label for this gene is obgE

Identifier: 86747377

GI number: 86747377

Start: 283922

End: 284983

Strand: Direct

Name: obgE

Synonym: RPB_0251

Alternate gene names: 86747377

Gene position: 283922-284983 (Clockwise)

Preceding gene: 86747375

Following gene: 86747378

Centisome position: 5.33

GC content: 66.95

Gene sequence:

>1062_bases
ATGAAATTCCTCGATGAAGCCAAGGTTTACATCCGCTCCGGCGACGGCGGGAACGGCTGCGTCGCGTTCCGCCGCGAGAA
GTTCATCGAGTTCGGCGGGCCCAATGGCGGCAATGGCGGCCGCGGCGGCGACGTCGTGGTCGAAGCGGCCGACGGCCTCA
ACACGCTGATCGACTATCGCTACCAGCAGCACTTCAAGGCCCAGAAGGGCGTCAACGGCATGGGCAGCGACCGCCACGGC
GCCAACGGCAAGGCCATCGTGCTGAAGGTCCCGGTCGGCACCCAGATCTTCGACGAGGACAAGGAAACGCTGATCCACGA
CTTCACCACGGTCGGGGAACGCTTCGTGCTGGCCGAAGGCGGCAATGGCGGCTTCGGCAACGCGCATTTCAAATCCTCGA
CCAACCGCGCTCCGCGCCACGCCAATCCGGGCCTGCCCGGCGAGGAGCGCTGGATCTGGCTGCGGCTGAAGCTGATCGCG
GATGCCGGCCTGGTCGGTCTGCCGAACGCCGGCAAATCGACGTTTCTCTCCAAGGTCAGCGCCGCCAAGCCGAAGATCGC
GGACTATCCGTTCACCACGCTGCATCCGCAGCTCGGCGTGGTGAATGCCGACGGCCGCGAATTCGTGCTGGCCGACATTC
CCGGGTTGATCGAGGGCGCCCATGAAGGCGCCGGCCTCGGCGACCGTTTCCTCGGCCATGTCGAGCGCTGTCGCGTGCTG
CTGCATCTGATCGACGCCACCTGCGAGCACGCCGGCAAGGCCTACAAGACAGTGCGCGGCGAACTCGAAGCCTATGCGGA
GACGCTCGCCGACAAGATCGAGATCGTGGCGCTGAACAAGATCGACGCGGTCGAGCCCGACGAGCTCAAGAAGCAGAAGG
ACCGGCTCAAGCGCGCGGCGAAGAAGACGCCGCTGCTGATCTCCGGCATCACCGGGCAGGGCGTGCCGGAAGCGCTGCGC
GCGCTGGCCGCCGTGATCGGCGAAGCGCCGGTCTCCGACAAGGCCAAGGGTGCCGCCGACAACGCGGCCAACGCCGAGCC
CTGGGCGCCGCAGGACGCCTGA

Upstream 100 bases:

>100_bases
GGCCGACCGCGGCTCACGCAAATCGCCGCACGGCGAAAGCGACGCAGTTCCGGCGATCGAGTTGTTGCCGCGCCCCCTCC
CTGTACGATACGGATAGCGC

Downstream 100 bases:

>100_bases
TCGGCGCCTGCCACTTTGGCGCAAGGCCGGCCCGGCGCGCTTGCACCCGCCGGTTCGGTGCGGCAAAACCAACCCGCCCG
TCGACGGCGCGCCGCCGATG

Product: GTPase ObgE

Products: NA

Alternate protein names: GTP-binding protein obg

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPNGGNGGRGGDVVVEAADGLNTLIDYRYQQHFKAQKGVNGMGSDRHG
ANGKAIVLKVPVGTQIFDEDKETLIHDFTTVGERFVLAEGGNGGFGNAHFKSSTNRAPRHANPGLPGEERWIWLRLKLIA
DAGLVGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPQLGVVNADGREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
LHLIDATCEHAGKAYKTVRGELEAYAETLADKIEIVALNKIDAVEPDELKKQKDRLKRAAKKTPLLISGITGQGVPEALR
ALAAVIGEAPVSDKAKGAADNAANAEPWAPQDA

Sequences:

>Translated_353_residues
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPNGGNGGRGGDVVVEAADGLNTLIDYRYQQHFKAQKGVNGMGSDRHG
ANGKAIVLKVPVGTQIFDEDKETLIHDFTTVGERFVLAEGGNGGFGNAHFKSSTNRAPRHANPGLPGEERWIWLRLKLIA
DAGLVGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPQLGVVNADGREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
LHLIDATCEHAGKAYKTVRGELEAYAETLADKIEIVALNKIDAVEPDELKKQKDRLKRAAKKTPLLISGITGQGVPEALR
ALAAVIGEAPVSDKAKGAADNAANAEPWAPQDA
>Mature_353_residues
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPNGGNGGRGGDVVVEAADGLNTLIDYRYQQHFKAQKGVNGMGSDRHG
ANGKAIVLKVPVGTQIFDEDKETLIHDFTTVGERFVLAEGGNGGFGNAHFKSSTNRAPRHANPGLPGEERWIWLRLKLIA
DAGLVGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPQLGVVNADGREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
LHLIDATCEHAGKAYKTVRGELEAYAETLADKIEIVALNKIDAVEPDELKKQKDRLKRAAKKTPLLISGITGQGVPEALR
ALAAVIGEAPVSDKAKGAADNAANAEPWAPQDA

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI24308117, Length=327, Percent_Identity=37.6146788990826, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI111955139, Length=347, Percent_Identity=37.7521613832853, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI111955063, Length=181, Percent_Identity=39.7790055248619, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI4758796, Length=113, Percent_Identity=36.283185840708, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557537, Length=88, Percent_Identity=39.7727272727273, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI58761500, Length=144, Percent_Identity=31.25, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789574, Length=337, Percent_Identity=51.0385756676558, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1790615, Length=170, Percent_Identity=31.7647058823529, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508313, Length=281, Percent_Identity=42.3487544483986, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17552324, Length=309, Percent_Identity=31.3915857605178, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17509631, Length=109, Percent_Identity=34.8623853211009, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17555344, Length=106, Percent_Identity=35.8490566037736, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71981008, Length=87, Percent_Identity=37.9310344827586, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321962, Length=158, Percent_Identity=41.7721518987342, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319281, Length=137, Percent_Identity=32.8467153284672, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321612, Length=100, Percent_Identity=37, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129375, Length=325, Percent_Identity=37.5384615384615, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24585318, Length=292, Percent_Identity=38.3561643835616, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640873, Length=144, Percent_Identity=31.9444444444444, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640877, Length=144, Percent_Identity=31.9444444444444, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640875, Length=144, Percent_Identity=31.9444444444444, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356473, Length=88, Percent_Identity=38.6363636363636, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17981711, Length=113, Percent_Identity=34.5132743362832, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OBG_RHOP2 (Q2J3J8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483873.1
- HSSP:   Q5SJ29
- ProteinModelPortal:   Q2J3J8
- STRING:   Q2J3J8
- GeneID:   3907879
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0251
- eggNOG:   COG0536
- HOGENOM:   HBG716038
- OMA:   ATCEHAG
- ProtClustDB:   PRK12299
- BioCyc:   RPAL316058:RPB_0251-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01454
- InterPro:   IPR014100
- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917
- InterPro:   IPR005225
- Gene3D:   G3DSA:2.70.210.12
- PANTHER:   PTHR11702:SF3
- PIRSF:   PIRSF002401
- PRINTS:   PR00326
- TIGRFAMs:   TIGR02729
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01018 GTP1_OBG; PF01926 MMR_HSR1; SSF82051 GTP1_OBG_sub

EC number: NA

Molecular weight: Translated: 37599; Mature: 37599

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPNGGNGGRGGDVVVEAADGLNTLIDYR
CCCCCCCEEEEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
YQQHFKAQKGVNGMGSDRHGANGKAIVLKVPVGTQIFDEDKETLIHDFTTVGERFVLAEG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHCCHHHHHHHHHHHHCCEEEEEEC
GNGGFGNAHFKSSTNRAPRHANPGLPGEERWIWLRLKLIADAGLVGLPNAGKSTFLSKVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHH
AAKPKIADYPFTTLHPQLGVVNADGREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
HCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHH
LHLIDATCEHAGKAYKTVRGELEAYAETLADKIEIVALNKIDAVEPDELKKQKDRLKRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KKTPLLISGITGQGVPEALRALAAVIGEAPVSDKAKGAADNAANAEPWAPQDA
HCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPNGGNGGRGGDVVVEAADGLNTLIDYR
CCCCCCCEEEEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
YQQHFKAQKGVNGMGSDRHGANGKAIVLKVPVGTQIFDEDKETLIHDFTTVGERFVLAEG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHCCHHHHHHHHHHHHCCEEEEEEC
GNGGFGNAHFKSSTNRAPRHANPGLPGEERWIWLRLKLIADAGLVGLPNAGKSTFLSKVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHH
AAKPKIADYPFTTLHPQLGVVNADGREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
HCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHH
LHLIDATCEHAGKAYKTVRGELEAYAETLADKIEIVALNKIDAVEPDELKKQKDRLKRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHH
KKTPLLISGITGQGVPEALRALAAVIGEAPVSDKAKGAADNAANAEPWAPQDA
HCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA