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The map label for this gene is proA

Identifier: 86747379

GI number: 86747379

Start: 286398

End: 287690

Strand: Direct

Name: proA

Synonym: RPB_0253

Alternate gene names: 86747379

Gene position: 286398-287690 (Clockwise)

Preceding gene: 86747378

Following gene: 86747380

Centisome position: 5.37

GC content: 68.91

Gene sequence:

>1293_bases
ATGACCGCCTCGCTCAAAGCCATCGACGGCAGCGCCGAGCTCGCGACGCTGATGCACGACCTCGGACGCCGCGCGCGCGC
TGCGGCGCGGGTGCTGGCGCTGGCCTCGCCGGAGCAGAAGAACCGCGCGCTGGAGGCGATGGAGCGGGCGATCCGGGCCG
GCGCCGACAAGATTTTGGCCGCCAATGCCGAGGACGTCGCCGACGCCAACGCCTCGGGCGCCAATTCCGCCTTCCTCGAC
CGGCTGACGCTGACGCCCGCGCGGGTCGAAGCCATGGCCGAAGGCATCGCCGTGGTCCGCGGCATTCCTGATCCGGTCGG
CGCCGTCACCGAAAGCTGGCAGCGGCCGAACGGCATGACGATCGAGCGCGTCCGGGTGCCGCTCGGCGTCGTCGCGGTGA
TCTACGAGAGCCGCCCCAACGTCACCGCCGATGCCGGCGTGCTGTGCCTGAAATCCGGCAATGCGGTGATCCTGCGCGGC
GGCTCGGAGAGTTTTCGCTCCGGCCGTGCGATCCACGACTGTCTGGTGCAGGGTCTTCGCGAGGCGGGGTTGCCGGAAGC
CGCTATCACGCTGGTGCCGACCCGCGATCGCGCCGCGGTGGGCTTGTTACTTGGCGGGCTGAACGGCTCTGTCGACGTGA
TCGTGCCGCGCGGCGGCAAGAGCCTGGTGGCGCGGGTCGAGAGTGAAGCGCGCGTGCCGGTATTCGCGCATCTCGAAGGC
GTCAACCACGTCTATATCGATCGCAGCGCCGATCTCGACATGGCGAAGTCGATCGTGCTCAACGCCAAGATGCGGCGCAC
CGGCGTGTGCGGCGCCGCCGAGACGCTGCTGATCGACCGCGCTGCCGCTGATACGCATCTCGCGCCACTGGTGGCGATGC
TGATCGACGCCGGCTGCGAAGTGCGCGGCGACGCCATCGTGCAGCACGCCGATGCGCGGGTGAAGCCCGCGACCGATCAG
GACTGGGACACCGAATATCTCGACGCGGTGATCGCCGCGAAGGTGGTCGACGACGTCGACGATGCGATCGTGCATATCCA
CGATCACGGCTCGCACCACACCGACGCGATCGTCGCCGAGGATGCGCAGACCGCCGCGAAATTTCTCGGCGAGGTCGATT
CCGCGATCGTGCTGCACAACGCCTCGACCCAGTTCGCCGACGGCGGCGAGTTCGGCTTCGGTGCCGAGATCGGCATCGCC
ACCGGCAAGTTCCACGCCCGTGGCCCGGTCGGCGCCGAGCAGCTCACGACCTTCAAGTATCGCATTCACGGCACCGGGCA
GACCCGGCCGTGA

Upstream 100 bases:

>100_bases
CGCGCGAGTATTCGCCCGGTTTCTCCGCAACCAGCTTGGCGATCCGGCATTTTCCGCCTATTTACATGATCAGACAGGTA
TTTTTCAGGCCGAACCGCCC

Downstream 100 bases:

>100_bases
GCAACCCGACCCGCCGGCGCGGAGCCTGAATTGACGCCCGATCCTGTGGCGGCCCGCCGCGCCGTTCCGGCCTACAGCCC
CGGGATGCGGATCGGCCTGC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTASLKAIDGSAELATLMHDLGRRARAAARVLALASPEQKNRALEAMERAIRAGADKILAANAEDVADANASGANSAFLD
RLTLTPARVEAMAEGIAVVRGIPDPVGAVTESWQRPNGMTIERVRVPLGVVAVIYESRPNVTADAGVLCLKSGNAVILRG
GSESFRSGRAIHDCLVQGLREAGLPEAAITLVPTRDRAAVGLLLGGLNGSVDVIVPRGGKSLVARVESEARVPVFAHLEG
VNHVYIDRSADLDMAKSIVLNAKMRRTGVCGAAETLLIDRAAADTHLAPLVAMLIDAGCEVRGDAIVQHADARVKPATDQ
DWDTEYLDAVIAAKVVDDVDDAIVHIHDHGSHHTDAIVAEDAQTAAKFLGEVDSAIVLHNASTQFADGGEFGFGAEIGIA
TGKFHARGPVGAEQLTTFKYRIHGTGQTRP

Sequences:

>Translated_430_residues
MTASLKAIDGSAELATLMHDLGRRARAAARVLALASPEQKNRALEAMERAIRAGADKILAANAEDVADANASGANSAFLD
RLTLTPARVEAMAEGIAVVRGIPDPVGAVTESWQRPNGMTIERVRVPLGVVAVIYESRPNVTADAGVLCLKSGNAVILRG
GSESFRSGRAIHDCLVQGLREAGLPEAAITLVPTRDRAAVGLLLGGLNGSVDVIVPRGGKSLVARVESEARVPVFAHLEG
VNHVYIDRSADLDMAKSIVLNAKMRRTGVCGAAETLLIDRAAADTHLAPLVAMLIDAGCEVRGDAIVQHADARVKPATDQ
DWDTEYLDAVIAAKVVDDVDDAIVHIHDHGSHHTDAIVAEDAQTAAKFLGEVDSAIVLHNASTQFADGGEFGFGAEIGIA
TGKFHARGPVGAEQLTTFKYRIHGTGQTRP
>Mature_429_residues
TASLKAIDGSAELATLMHDLGRRARAAARVLALASPEQKNRALEAMERAIRAGADKILAANAEDVADANASGANSAFLDR
LTLTPARVEAMAEGIAVVRGIPDPVGAVTESWQRPNGMTIERVRVPLGVVAVIYESRPNVTADAGVLCLKSGNAVILRGG
SESFRSGRAIHDCLVQGLREAGLPEAAITLVPTRDRAAVGLLLGGLNGSVDVIVPRGGKSLVARVESEARVPVFAHLEGV
NHVYIDRSADLDMAKSIVLNAKMRRTGVCGAAETLLIDRAAADTHLAPLVAMLIDAGCEVRGDAIVQHADARVKPATDQD
WDTEYLDAVIAAKVVDDVDDAIVHIHDHGSHHTDAIVAEDAQTAAKFLGEVDSAIVLHNASTQFADGGEFGFGAEIGIAT
GKFHARGPVGAEQLTTFKYRIHGTGQTRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=411, Percent_Identity=37.956204379562, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI62912457, Length=411, Percent_Identity=37.956204379562, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=41.9117647058824, Blast_Score=326, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17569969, Length=420, Percent_Identity=35.4761904761905, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17569967, Length=420, Percent_Identity=35.4761904761905, Blast_Score=247, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324899, Length=430, Percent_Identity=37.906976744186, Blast_Score=266, Evalue=4e-72,
Organism=Drosophila melanogaster, GI21357643, Length=407, Percent_Identity=36.3636363636364, Blast_Score=242, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_RHOP2 (Q2J3J6)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_483875.1
- HSSP:   P54886
- ProteinModelPortal:   Q2J3J6
- SMR:   Q2J3J6
- STRING:   Q2J3J6
- GeneID:   3907881
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0253
- eggNOG:   COG0014
- HOGENOM:   HBG318080
- OMA:   ITHINRY
- ProtClustDB:   PRK00197
- BioCyc:   RPAL316058:RPB_0253-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45108; Mature: 44976

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASLKAIDGSAELATLMHDLGRRARAAARVLALASPEQKNRALEAMERAIRAGADKILA
CCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
ANAEDVADANASGANSAFLDRLTLTPARVEAMAEGIAVVRGIPDPVGAVTESWQRPNGMT
CCCHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCE
IERVRVPLGVVAVIYESRPNVTADAGVLCLKSGNAVILRGGSESFRSGRAIHDCLVQGLR
EEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCHHHHCCCHHHHHHHHHHH
EAGLPEAAITLVPTRDRAAVGLLLGGLNGSVDVIVPRGGKSLVARVESEARVPVFAHLEG
HCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEECC
VNHVYIDRSADLDMAKSIVLNAKMRRTGVCGAAETLLIDRAAADTHLAPLVAMLIDAGCE
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEHHCCCHHHHHHHHHHHHCCCC
VRGDAIVQHADARVKPATDQDWDTEYLDAVIAAKVVDDVDDAIVHIHDHGSHHTDAIVAE
CCCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEC
DAQTAAKFLGEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKFHARGPVGAEQLTTFKY
CHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCEECCCCCCHHHEEEEEE
RIHGTGQTRP
EEECCCCCCC
>Mature Secondary Structure 
TASLKAIDGSAELATLMHDLGRRARAAARVLALASPEQKNRALEAMERAIRAGADKILA
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEE
ANAEDVADANASGANSAFLDRLTLTPARVEAMAEGIAVVRGIPDPVGAVTESWQRPNGMT
CCCHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCE
IERVRVPLGVVAVIYESRPNVTADAGVLCLKSGNAVILRGGSESFRSGRAIHDCLVQGLR
EEEEECCCEEEEEEEECCCCCCCCCCEEEEECCCEEEEECCCHHHHCCCHHHHHHHHHHH
EAGLPEAAITLVPTRDRAAVGLLLGGLNGSVDVIVPRGGKSLVARVESEARVPVFAHLEG
HCCCCCCEEEEEECCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEECC
VNHVYIDRSADLDMAKSIVLNAKMRRTGVCGAAETLLIDRAAADTHLAPLVAMLIDAGCE
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEHHCCCHHHHHHHHHHHHCCCC
VRGDAIVQHADARVKPATDQDWDTEYLDAVIAAKVVDDVDDAIVHIHDHGSHHTDAIVAE
CCCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEC
DAQTAAKFLGEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKFHARGPVGAEQLTTFKY
CHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCEECCCCCCHHHEEEEEE
RIHGTGQTRP
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA