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The map label for this gene is mrcA [H]

Identifier: 78066728

GI number: 78066728

Start: 2314205

End: 2316721

Strand: Reverse

Name: mrcA [H]

Synonym: Bcep18194_A5259

Alternate gene names: 78066728

Gene position: 2316721-2314205 (Counterclockwise)

Preceding gene: 78066733

Following gene: 78066723

Centisome position: 62.71

GC content: 66.67

Gene sequence:

>2517_bases
ATGCCAATTATCAAACGACCGCCTTCTTCTTCCGACAGGAACGAACCCCACTACACGCTGCGCCGTTCACCGTCCGGAGC
CTATTACCCCGATGACGACGATCGCGACGACGACCGCCGCACCCAGCGCAGCGGCGGCGGAGGGCGCGGTCGCTCGTTCG
GCTCGCGCGTCATGCTGTGGTTCGCCGGCCTGTTCGTGACGCTGGCGATCGTCGGCGCGCTGATCGTCGGCTACGCGCTG
GTCGTGATGGCGCCGCAGCTGCCGTCGCTCGATGCGCTGACCAACTACCAGCCGAAAGTACCGTTGCGCGTGTTCACGGC
CGATCACGTGTTGATCGGCGAGTTCGGCGAAGAGCGCCGCAGCCTCGTGCGCTTCCAGGAAATCCCGGACGTGATGAAGA
AGGCTGTGCTCGCGATCGAGGACTACCGCTTCTACGAACACGGCGGCGTCGACTTCGTCGGTATCCTGCGCGCGGGTGTC
GCCGACCTGATGCACGGCGGCGCGCGCCAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGTCGAG
CGAGAAGACCTACACGCGCAAGATTTACGAAATGCTGCTCGCGTACAAGATCGAGAGGGCCCTGACGAAGGACCAGATCC
TCGAGCTGTACATGAACCAGATCTATCTCGGCCAGCGTTCGTACGGCTTCGCGGCCGCCGCGCGCGTGTATTTCGGCAAG
GACCTGAAGGACATCACGCTCGCGGAAGCGGCGATGCTCGCGGGGCTGCCGAAGGCGCCGTCCGCGTATAACCCGGTCGT
CAATCCGAAGCGCGCGAAGGTGCGCCAGGAATACATCCTGAAGCGCATGCTCGAGGTCGGCTACATCACGCAGCCGCAGT
ACGACGAAGCCGTGAAGGAAGAGATCCACGTGCGCACGCCGGGCAACCAGTACGCGGTGCACGGCGAGTACGTCGCCGAG
ATGGTGCGGCAGATGATGTACCAGCAGTACAAGGATGAAACCTATACGCGCGGGCTGACCGTCACGACGACGATCAACTC
GTCCGACCAGGAAGCCGCGTACCAGGCCGTGCGTCGCGGCATTCTCGACTACGAGCGCCGCCACGGCTATCGCGGGCCGG
AAGCGACGATCAAGCTGCCCGCGGCCGGCGACGACCGCGACGAGGCGATCGACGACGCGCTCGCCGATCACCCGGACAAC
GGCGACCTGCAGTCGGCAGTGGTGCTGTCGGCGTCGCCGAATGCGGTCGAGGTGCAGTTCGTCGGCGGCGCGACCACGAC
GATCGGCCCGGCCGGGCTGCGCTTCGTGGCGGGCGCGCTCAGCCCCCGCGCGAACGACACGCTGCGCATCAAGCCGGGCT
CGGTCGTGCGCGTGCTGAAGGACGGCAAGACGGGCTGGCAGGTCGTGCAACTGCCGCAGGTCGAAGGCGCGCTCGTCGCG
ATGGCGCCGCAGGATGGCGCGATCCGCTCGCTCGTCGGCGGCTTCGACTTCAACAAGAGCAAGTTCAACCACGTGACGCA
GGCATGGCGGCAGCCGGGCTCGTCGTTCAAGCCGTTCATCTACTCGGCATCGCTCGACAAGGGCATGGGGCCGGCGACGA
TGATCAACGACGCGCCGCTGTACTTCCCGCCGAGCGTGCCGGGCGGCACCGCATGGGAGCCGAAGGACGACGACCAGCCG
GATGGCCCGATGACGATGCGCACCGGCCTGCAGCGTTCGAAGAACCTCGTGTCGATCCGGATCCTCGCGTCGATCGGTAC
GCAGTATGCGCAGCAGTACGTGACGCAGCGCTTCGGCTTCGATCCCGCGAAGACGCCGCCTTACCTGCCGATGGCACTCG
GCGCGGGCCTCGTGACGCCGCTGCAGCTCGCGACCGGCTATGCGGTGTTCGCGAACGGCGGCTACAAGGTCGATCCGTAC
CTGATCGCCGAGGTCGACGATGCACGCGGCCAGGCGCTGCAGAAGTCGCAGCCGGTGCTCGCGGGCAGCACGGCACCGCG
CACGATCGAAGGCCGCAACGCGTACGTGATGAACAGCCTGCTGCATTCGGTCGCGACGGCCGGCACCGGCGCGGGCACCA
ATGCACTGGGCCGCAGCGACCTGCAGGGCAAGACGGGTACGACCAACGACGCGAAGGACGGCTGGTTCGCCGGCTACCAG
CAGTCGCTCGTCGCGGTCGCGTGGATGGGCTTCGACCAGCCGAAGAGCCTCGGCAGCCGCGAATTCGGCGCACAGCTCGC
GTTGCCGATCTGGGTGAACTACATGCGCACCGCGCTGAACGGCGTACCCGAACAGCAGATGCCGATGCCGGACGGCCTGA
CTTCGATCGACGGCGAGCTGTACTACGCCGATCGCACGCCGGGCAATGGCTTCGTCGCGAACGTCGACATCAACCCGGCC
GAGAATTCGATCAGCGCGAACGACGCACTCGGTTCGGCCGGCGCGGCGGGGCTGGCGCCGCCGCCCGTCACGCCGCAGGA
GAAGCAGCAGATCATGGACATGTTCGAGAGCAAGTAA

Upstream 100 bases:

>100_bases
TCTGTCGACGTGACAGCCGCATCACGCTGTATCCGCTCTGATTCATTCGCCTGCATTTCCGCATGCGTCCGAGGGCGCCA
CGTACGCCCGCCATTCCGAC

Downstream 100 bases:

>100_bases
GCGGCACGCCGCAGTTCCGGCATGCGACGGGCGCCTTCGGGCGCCCGTTTTTTTTGCGCGAATTGTCGGCGGAGAGAAGG
GGAATCAGAACGACGCGCAG

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 838; Mature: 837

Protein sequence:

>838_residues
MPIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDDRRTQRSGGGGRGRSFGSRVMLWFAGLFVTLAIVGALIVGYAL
VVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERRSLVRFQEIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGV
ADLMHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRSYGFAAAARVYFGK
DLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEVGYITQPQYDEAVKEEIHVRTPGNQYAVHGEYVAE
MVRQMMYQQYKDETYTRGLTVTTTINSSDQEAAYQAVRRGILDYERRHGYRGPEATIKLPAAGDDRDEAIDDALADHPDN
GDLQSAVVLSASPNAVEVQFVGGATTTIGPAGLRFVAGALSPRANDTLRIKPGSVVRVLKDGKTGWQVVQLPQVEGALVA
MAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGMGPATMINDAPLYFPPSVPGGTAWEPKDDDQP
DGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGFDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPY
LIAEVDDARGQALQKSQPVLAGSTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRSDLQGKTGTTNDAKDGWFAGYQ
QSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTSIDGELYYADRTPGNGFVANVDINPA
ENSISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK

Sequences:

>Translated_838_residues
MPIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDDRRTQRSGGGGRGRSFGSRVMLWFAGLFVTLAIVGALIVGYAL
VVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERRSLVRFQEIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGV
ADLMHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRSYGFAAAARVYFGK
DLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEVGYITQPQYDEAVKEEIHVRTPGNQYAVHGEYVAE
MVRQMMYQQYKDETYTRGLTVTTTINSSDQEAAYQAVRRGILDYERRHGYRGPEATIKLPAAGDDRDEAIDDALADHPDN
GDLQSAVVLSASPNAVEVQFVGGATTTIGPAGLRFVAGALSPRANDTLRIKPGSVVRVLKDGKTGWQVVQLPQVEGALVA
MAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGMGPATMINDAPLYFPPSVPGGTAWEPKDDDQP
DGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGFDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPY
LIAEVDDARGQALQKSQPVLAGSTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRSDLQGKTGTTNDAKDGWFAGYQ
QSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTSIDGELYYADRTPGNGFVANVDINPA
ENSISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK
>Mature_837_residues
PIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDDRRTQRSGGGGRGRSFGSRVMLWFAGLFVTLAIVGALIVGYALV
VMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERRSLVRFQEIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVA
DLMHGGARQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRSYGFAAAARVYFGKD
LKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEVGYITQPQYDEAVKEEIHVRTPGNQYAVHGEYVAEM
VRQMMYQQYKDETYTRGLTVTTTINSSDQEAAYQAVRRGILDYERRHGYRGPEATIKLPAAGDDRDEAIDDALADHPDNG
DLQSAVVLSASPNAVEVQFVGGATTTIGPAGLRFVAGALSPRANDTLRIKPGSVVRVLKDGKTGWQVVQLPQVEGALVAM
APQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGMGPATMINDAPLYFPPSVPGGTAWEPKDDDQPD
GPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGFDPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPYL
IAEVDDARGQALQKSQPVLAGSTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRSDLQGKTGTTNDAKDGWFAGYQQ
SLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTSIDGELYYADRTPGNGFVANVDINPAE
NSISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=818, Percent_Identity=39.7310513447433, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1786343, Length=265, Percent_Identity=35.4716981132075, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1788867, Length=229, Percent_Identity=31.8777292576419, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=34.0909090909091, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 91311; Mature: 91179

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDDRRTQRSGGGGRGRSFGSRVMLW
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
FAGLFVTLAIVGALIVGYALVVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCEEEECHHHHHHH
SLVRFQEIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVADLMHGGARQGASTITMQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
RNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRSYGFAAAARVYFGK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEVGYITQPQYDEAVKE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EIHVRTPGNQYAVHGEYVAEMVRQMMYQQYKDETYTRGLTVTTTINSSDQEAAYQAVRRG
CEEEECCCCCEEECHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHHHHHH
ILDYERRHGYRGPEATIKLPAAGDDRDEAIDDALADHPDNGDLQSAVVLSASPNAVEVQF
HHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEE
VGGATTTIGPAGLRFVAGALSPRANDTLRIKPGSVVRVLKDGKTGWQVVQLPQVEGALVA
ECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCCEEEEECCCCCEEEE
MAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGMGPATMINDAPL
ECCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCCCE
YFPPSVPGGTAWEPKDDDQPDGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGF
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCC
DPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPYLIAEVDDARGQALQKSQPVL
CCCCCCCCCHHHHCCCHHHHHHHHCCEEEEECCCEEECCEEEEEECCHHCHHHHHCCCEE
AGSTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRSDLQGKTGTTNDAKDGWFAGYQ
ECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTSIDGEL
HHHHHHHHCCCCCCHHCCCHHHCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
YYADRTPGNGFVANVDINPAENSISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK
EEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIIKRPPSSSDRNEPHYTLRRSPSGAYYPDDDDRDDDRRTQRSGGGGRGRSFGSRVMLW
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
FAGLFVTLAIVGALIVGYALVVMAPQLPSLDALTNYQPKVPLRVFTADHVLIGEFGEERR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCEEEECHHHHHHH
SLVRFQEIPDVMKKAVLAIEDYRFYEHGGVDFVGILRAGVADLMHGGARQGASTITMQVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
RNFFLSSEKTYTRKIYEMLLAYKIERALTKDQILELYMNQIYLGQRSYGFAAAARVYFGK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
DLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKVRQEYILKRMLEVGYITQPQYDEAVKE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EIHVRTPGNQYAVHGEYVAEMVRQMMYQQYKDETYTRGLTVTTTINSSDQEAAYQAVRRG
CEEEECCCCCEEECHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHHHHHH
ILDYERRHGYRGPEATIKLPAAGDDRDEAIDDALADHPDNGDLQSAVVLSASPNAVEVQF
HHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEE
VGGATTTIGPAGLRFVAGALSPRANDTLRIKPGSVVRVLKDGKTGWQVVQLPQVEGALVA
ECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCCEEEEECCCCCEEEE
MAPQDGAIRSLVGGFDFNKSKFNHVTQAWRQPGSSFKPFIYSASLDKGMGPATMINDAPL
ECCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECCCCE
YFPPSVPGGTAWEPKDDDQPDGPMTMRTGLQRSKNLVSIRILASIGTQYAQQYVTQRFGF
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCC
DPAKTPPYLPMALGAGLVTPLQLATGYAVFANGGYKVDPYLIAEVDDARGQALQKSQPVL
CCCCCCCCCHHHHCCCHHHHHHHHCCEEEEECCCEEECCEEEEEECCHHCHHHHHCCCEE
AGSTAPRTIEGRNAYVMNSLLHSVATAGTGAGTNALGRSDLQGKTGTTNDAKDGWFAGYQ
ECCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QSLVAVAWMGFDQPKSLGSREFGAQLALPIWVNYMRTALNGVPEQQMPMPDGLTSIDGEL
HHHHHHHHCCCCCCHHCCCHHHCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
YYADRTPGNGFVANVDINPAENSISANDALGSAGAAGLAPPPVTPQEKQQIMDMFESK
EEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA