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The map label for this gene is metK [H]

Identifier: 77461490

GI number: 77461490

Start: 5929733

End: 5930923

Strand: Direct

Name: metK [H]

Synonym: Pfl01_5269

Alternate gene names: 77461490

Gene position: 5929733-5930923 (Clockwise)

Preceding gene: 77461489

Following gene: 77461491

Centisome position: 92.1

GC content: 61.96

Gene sequence:

>1191_bases
ATGAGCGAATACTCCCTCTTCACCTCCGAGTCCGTGTCTGAAGGACATCCGGACAAAATCGCCGACCAGATTTCCGATGC
AGTGCTGGACGCCATCATCGCCCAGGACAAGCACGCACGCGTTGCGGTGGAAACCCTGGTCAAGACGGGCGTGGCCATCG
TTGCCGGTGAAGTGACCACCAGCGCCTGGGTCGACCTGGAGCAGATCGTTCGTGACGTGATCTGCGACATCGGCTACACC
AGCTCCGACGTCGGCTTCGACGGCGCGACCTGCGGCGTGATGAACATCATCGGCAAGCAGTCCCCTGACATCAACCAGGG
TGTTGACCGTGCCAAGCCTGAAGATCAGGGCGCCGGCGACCAGGGCCTGATGTTCGGCTACGCCAGCAACGAAACCGACG
TGCTGATGCCGGCACCGATCACCTTCTCGCACCAGCTGGTACAGCGTCAGGCCGAAGCCCGTAAATCGGGTCTGCTGCCT
TGGCTGCGTCCGGACGCCAAGTCGCAAGTCACCTGCCGTTACGAAGGCGGCAAGGTTGTCGGTATCGACGCCGTGGTTCT
GTCGACCCAGCACAACCCGGAAGTGTCGTACAACGACCTGCGCGAAGGCGTGATGGAGCTGATCGTCAAGCACGTGCTGC
CTGCCGAACTGCTGAGCAAGGACACCCAGTTCCACATCAACCCGACCGGCCAGTTCATCATTGGCGGCCCGGTAGGTGAC
TGCGGTCTGACCGGTCGCAAGATCATCGTCGACAGCTACGGCGGCATGGCCCGTCACGGCGGCGGCGCGTTCTCCGGTAA
GGATCCATCCAAGGTTGACCGTTCGGCGGCCTACGCTGGCCGTTACGTTGCCAAGAACATCGTGGCGGCCGGCCTGGCCG
AGCGTTGCGAGATTCAGGTTTCCTACGCGATCGGTGTGGCCCAGCCTACCTCGATCTCGCTGAACACCTTCGGCACCGGC
AAGATCAGCGACGACAAGATCATCAAACTGGTCCGCGAAGTGTTCGACCTGCGTCCATACGCGATCACCACCATGCTGGA
TCTGCTGCACCCGATGTACCAGGAAACCGCAGCCTACGGCCACTTCGGCCGTGCTCCGCAGACCAAGACCGTTGGCGAAG
ACACCTTCTCCACGTTCACCTGGGAAAAAACCGACCGCGCCGACGCTCTGCGTTCCGCTGCCGGCCTGTAA

Upstream 100 bases:

>100_bases
CCTCTATGTAGGCTTACGTAATGGTTTCCAGATCCAGGTCCGCCATTTTCAGCGACCGGCTGGCGACACTCACCATCGGT
AAATTCAGGAAAACATCGAG

Downstream 100 bases:

>100_bases
TTTTCCTGGCGGTACAAGAAGCCCCGCACGGTTCGCCGTGCGGGGCTTTTTCATGGCCGCGAAACAACCGGCAAAACAGC
CGCCCGGCCCGCTTCAAAAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAQDKHARVAVETLVKTGVAIVAGEVTTSAWVDLEQIVRDVICDIGYT
SSDVGFDGATCGVMNIIGKQSPDINQGVDRAKPEDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLP
WLRPDAKSQVTCRYEGGKVVGIDAVVLSTQHNPEVSYNDLREGVMELIVKHVLPAELLSKDTQFHINPTGQFIIGGPVGD
CGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTG
KISDDKIIKLVREVFDLRPYAITTMLDLLHPMYQETAAYGHFGRAPQTKTVGEDTFSTFTWEKTDRADALRSAAGL

Sequences:

>Translated_396_residues
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAQDKHARVAVETLVKTGVAIVAGEVTTSAWVDLEQIVRDVICDIGYT
SSDVGFDGATCGVMNIIGKQSPDINQGVDRAKPEDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLP
WLRPDAKSQVTCRYEGGKVVGIDAVVLSTQHNPEVSYNDLREGVMELIVKHVLPAELLSKDTQFHINPTGQFIIGGPVGD
CGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTG
KISDDKIIKLVREVFDLRPYAITTMLDLLHPMYQETAAYGHFGRAPQTKTVGEDTFSTFTWEKTDRADALRSAAGL
>Mature_395_residues
SEYSLFTSESVSEGHPDKIADQISDAVLDAIIAQDKHARVAVETLVKTGVAIVAGEVTTSAWVDLEQIVRDVICDIGYTS
SDVGFDGATCGVMNIIGKQSPDINQGVDRAKPEDQGAGDQGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPW
LRPDAKSQVTCRYEGGKVVGIDAVVLSTQHNPEVSYNDLREGVMELIVKHVLPAELLSKDTQFHINPTGQFIIGGPVGDC
GLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQVSYAIGVAQPTSISLNTFGTGK
ISDDKIIKLVREVFDLRPYAITTMLDLLHPMYQETAAYGHFGRAPQTKTVGEDTFSTFTWEKTDRADALRSAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=383, Percent_Identity=54.5691906005222, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI5174529, Length=383, Percent_Identity=54.0469973890339, Blast_Score=391, Evalue=1e-109,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=72.3785166240409, Blast_Score=591, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=54.5691906005222, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=53.9845758354756, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=53.3678756476684, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17551082, Length=388, Percent_Identity=52.5773195876289, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=48.586118251928, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=51.4880952380952, Blast_Score=327, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI25145635, Length=339, Percent_Identity=50.1474926253687, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=314, Percent_Identity=50.6369426751592, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=99, Percent_Identity=56.5656565656566, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=50, Percent_Identity=68, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=53.5897435897436, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=53.2637075718016, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=383, Percent_Identity=51.4360313315927, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580529, Length=383, Percent_Identity=51.4360313315927, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580537, Length=383, Percent_Identity=51.4360313315927, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580533, Length=383, Percent_Identity=51.4360313315927, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580535, Length=383, Percent_Identity=51.4360313315927, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580531, Length=383, Percent_Identity=51.4360313315927, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580527, Length=383, Percent_Identity=51.4360313315927, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580525, Length=383, Percent_Identity=51.4360313315927, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=49.0909090909091, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=52.991452991453, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42528; Mature: 42397

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAQDKHARVAVETLVKTGVAIVAGEVTT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCHH
SAWVDLEQIVRDVICDIGYTSSDVGFDGATCGVMNIIGKQSPDINQGVDRAKPEDQGAGD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCC
QGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPWLRPDAKSQVTCRYEGGKVV
CCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEE
GIDAVVLSTQHNPEVSYNDLREGVMELIVKHVLPAELLSKDTQFHINPTGQFIIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
SYAIGVAQPTSISLNTFGTGKISDDKIIKLVREVFDLRPYAITTMLDLLHPMYQETAAYG
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
HFGRAPQTKTVGEDTFSTFTWEKTDRADALRSAAGL
CCCCCCCCCCCCCCHHCEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
SEYSLFTSESVSEGHPDKIADQISDAVLDAIIAQDKHARVAVETLVKTGVAIVAGEVTT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCHH
SAWVDLEQIVRDVICDIGYTSSDVGFDGATCGVMNIIGKQSPDINQGVDRAKPEDQGAGD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCC
QGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPWLRPDAKSQVTCRYEGGKVV
CCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEE
GIDAVVLSTQHNPEVSYNDLREGVMELIVKHVLPAELLSKDTQFHINPTGQFIIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
SYAIGVAQPTSISLNTFGTGKISDDKIIKLVREVFDLRPYAITTMLDLLHPMYQETAAYG
EEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
HFGRAPQTKTVGEDTFSTFTWEKTDRADALRSAAGL
CCCCCCCCCCCCCCHHCEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA