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The map label for this gene is ligB

Identifier: 77461491

GI number: 77461491

Start: 5931067

End: 5932743

Strand: Direct

Name: ligB

Synonym: Pfl01_5270

Alternate gene names: 77461491

Gene position: 5931067-5932743 (Clockwise)

Preceding gene: 77461490

Following gene: 77461492

Centisome position: 92.12

GC content: 64.88

Gene sequence:

>1677_bases
ATGCTTGCCACACTGCGCCTGTTTTTGTTTTTCCTGCCGTTTTTCCACCTCCACACCTTCGCTGACGACTGCCCCGACTG
GGCCGCCGCCCGGGCATCGAGCGAAGTCGTCGCCCTGCAACAACAGATCGATCGCTGGGACGACGCCTATCATCGCGAAG
GCCGCTCGGTGATCGCCGATGAGCTCTACGATCAGTCCCGCCTGCGCCTGAACCAATGGCGCCAGTGCTTCAATCTTCCT
TCTCCGCCCGAGCCGTTGCGCACCGCATCAGGCCCGGTTGCGCACCCGGTCGCTCACACCGGTCTGGACAAGCTTCACGA
CGCGGCGGACATCGCCACCTGGCTGCGCGACCGCCAAAACGTATGGGTGCAACCCAAGGTCGATGGCGTGGCGGTGACCC
TGATCTATCGCGACGGCCGATTGCATCAGGCGATCAGTCGCGGGGATGGTGTTCGCGGGCAGGACTGGACGGCATCTGCA
AAAAAGATCGGAGCCATTCCGCAACAGCTGACGCAACCGCAGGATCTGCTGGTGCAAGGCGAACTCTACTGGCGCCTGAA
CGGACACATGCAGGCACGTGCCGGCAGCGCCAACGCCCGCGCCACCGTGGCCGGCCTGCTGGGACGCAAGGATCTCGGCG
CGGAACACGCCGCCGGTATCGGGCTGTTTGTCTGGGACTGGCCGCACGGGCCGAGGGACTTGCCGGAGCGAATCACGGCC
CTGACACAATTCGGTTTTCCTACCACGGTGCCCTACACCCAGGCCGTCGCCAGCCTGAACGACGCGCAACACTGGCGCGA
TCACTGGTATCACTCGCCACTGCCATTCGCCACGGACGGCGTGGTTCTGCGCCAGAGCCTGCGACCGCCCGCCGAGCGCT
GGCAGGCTCGTCCGCCCTACTGGGCTGTCGCCTGGAAATACCCGTTCGCCCAGGCACTGGCCGATGTGCGCAAGGTGCAC
TTCAAGATCGGCCGCACCGGGCGCATTACGCCGGTGCTTGAACTGTCGCCGGTGATGCTCGATGACCGGCAGATCAAACG
GGTCAGCGTCAGTTCGCTCAGGCGTTGGCAGGAGCTGGACATTCGCCCCGGCGATCAGGTCGCAATCAGCCTGGCCGGGC
TGACGATTCCGCGCCTCGACAGCGTGGTACTGCGCAGTACCGAACGCGCCGATCTGAACGTTCCGCTTGCCAGCGATTTT
CACGCCCTGAGCTGCTGGCAACCGACACCGGGCTGCGAGAGTCAGTTCCTCGCGCGCCTGACCTGGCTCAGCGGCAAGCA
GGGACTGGCCCTGCCGCATGTCGGTCGCGGCACCTGGGAGAAACTTCTCGAAACAGGCCGCCTGAACAGCCTGCTGGATT
GGTTGACCCTCGACGGGCCGGAGCTTGCTAACATTGCCGGTCTCGGCGAGCGTAGCAGTGCCCGCCTGCTGCACAGTTTT
CACAGCGCCCGCCAACGGCCGTTCGCACAATGGCTCAAAGCCCTGGGGTTGCCACCGACCGGTCAGGCAACACTCGCCGA
TTCATGGCAGGCGCTGGCACAACGCAACACCGAACAATGGCAGGCAGAAGCCGGGATAGGCCCGGGTCGCGCGGCGCAAT
TGAGCGCCTTTTTTCGCGACCCGCAGGTACTGACCCTGAGTGAAACCCTGCAAGCCGCCGGCGTCGACGGCTTCTGA

Upstream 100 bases:

>100_bases
GGCTTTTTCATGGCCGCGAAACAACCGGCAAAACAGCCGCCCGGCCCGCTTCAAAATCCCGGCTAGCCTTCAGGTATCTC
TCTGAGCAAGGACGCTCACG

Downstream 100 bases:

>100_bases
ACACGCAATCCCCGGCCAGCCGGGAACCCAATGTCCGCCGGAGCGCTCCAACAGCGCAGTGCTTTACCGACCCGATTGCC
TTTGCACATGGAGCTTTTAT

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MLATLRLFLFFLPFFHLHTFADDCPDWAAARASSEVVALQQQIDRWDDAYHREGRSVIADELYDQSRLRLNQWRQCFNLP
SPPEPLRTASGPVAHPVAHTGLDKLHDAADIATWLRDRQNVWVQPKVDGVAVTLIYRDGRLHQAISRGDGVRGQDWTASA
KKIGAIPQQLTQPQDLLVQGELYWRLNGHMQARAGSANARATVAGLLGRKDLGAEHAAGIGLFVWDWPHGPRDLPERITA
LTQFGFPTTVPYTQAVASLNDAQHWRDHWYHSPLPFATDGVVLRQSLRPPAERWQARPPYWAVAWKYPFAQALADVRKVH
FKIGRTGRITPVLELSPVMLDDRQIKRVSVSSLRRWQELDIRPGDQVAISLAGLTIPRLDSVVLRSTERADLNVPLASDF
HALSCWQPTPGCESQFLARLTWLSGKQGLALPHVGRGTWEKLLETGRLNSLLDWLTLDGPELANIAGLGERSSARLLHSF
HSARQRPFAQWLKALGLPPTGQATLADSWQALAQRNTEQWQAEAGIGPGRAAQLSAFFRDPQVLTLSETLQAAGVDGF

Sequences:

>Translated_558_residues
MLATLRLFLFFLPFFHLHTFADDCPDWAAARASSEVVALQQQIDRWDDAYHREGRSVIADELYDQSRLRLNQWRQCFNLP
SPPEPLRTASGPVAHPVAHTGLDKLHDAADIATWLRDRQNVWVQPKVDGVAVTLIYRDGRLHQAISRGDGVRGQDWTASA
KKIGAIPQQLTQPQDLLVQGELYWRLNGHMQARAGSANARATVAGLLGRKDLGAEHAAGIGLFVWDWPHGPRDLPERITA
LTQFGFPTTVPYTQAVASLNDAQHWRDHWYHSPLPFATDGVVLRQSLRPPAERWQARPPYWAVAWKYPFAQALADVRKVH
FKIGRTGRITPVLELSPVMLDDRQIKRVSVSSLRRWQELDIRPGDQVAISLAGLTIPRLDSVVLRSTERADLNVPLASDF
HALSCWQPTPGCESQFLARLTWLSGKQGLALPHVGRGTWEKLLETGRLNSLLDWLTLDGPELANIAGLGERSSARLLHSF
HSARQRPFAQWLKALGLPPTGQATLADSWQALAQRNTEQWQAEAGIGPGRAAQLSAFFRDPQVLTLSETLQAAGVDGF
>Mature_558_residues
MLATLRLFLFFLPFFHLHTFADDCPDWAAARASSEVVALQQQIDRWDDAYHREGRSVIADELYDQSRLRLNQWRQCFNLP
SPPEPLRTASGPVAHPVAHTGLDKLHDAADIATWLRDRQNVWVQPKVDGVAVTLIYRDGRLHQAISRGDGVRGQDWTASA
KKIGAIPQQLTQPQDLLVQGELYWRLNGHMQARAGSANARATVAGLLGRKDLGAEHAAGIGLFVWDWPHGPRDLPERITA
LTQFGFPTTVPYTQAVASLNDAQHWRDHWYHSPLPFATDGVVLRQSLRPPAERWQARPPYWAVAWKYPFAQALADVRKVH
FKIGRTGRITPVLELSPVMLDDRQIKRVSVSSLRRWQELDIRPGDQVAISLAGLTIPRLDSVVLRSTERADLNVPLASDF
HALSCWQPTPGCESQFLARLTWLSGKQGLALPHVGRGTWEKLLETGRLNSLLDWLTLDGPELANIAGLGERSSARLLHSF
HSARQRPFAQWLKALGLPPTGQATLADSWQALAQRNTEQWQAEAGIGPGRAAQLSAFFRDPQVLTLSETLQAAGVDGF

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily

Homologues:

Organism=Escherichia coli, GI87082305, Length=539, Percent_Identity=42.4860853432282, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1788750, Length=567, Percent_Identity=26.63139329806, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIGB_PSEPF (Q3K5E7)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350998.1
- ProteinModelPortal:   Q3K5E7
- SMR:   Q3K5E7
- STRING:   Q3K5E7
- GeneID:   3715338
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5270
- eggNOG:   COG0272
- HOGENOM:   HBG336755
- OMA:   NARSKVA
- ProtClustDB:   PRK08097
- BioCyc:   PFLU205922:PFL_5270-MONOMER
- HAMAP:   MF_01587
- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00532

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 62268; Mature: 62268

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATLRLFLFFLPFFHLHTFADDCPDWAAARASSEVVALQQQIDRWDDAYHREGRSVIAD
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELYDQSRLRLNQWRQCFNLPSPPEPLRTASGPVAHPVAHTGLDKLHDAADIATWLRDRQN
HHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCC
VWVQPKVDGVAVTLIYRDGRLHQAISRGDGVRGQDWTASAKKIGAIPQQLTQPQDLLVQG
EEEEECCCCEEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHCCCHHEEEEC
ELYWRLNGHMQARAGSANARATVAGLLGRKDLGAEHAAGIGLFVWDWPHGPRDLPERITA
EEEEEECCCEEECCCCCCCHHHHHHHHCCHHCCCHHHCCCEEEEEECCCCCHHHHHHHHH
LTQFGFPTTVPYTQAVASLNDAQHWRDHWYHSPLPFATDGVVLRQSLRPPAERWQARPPY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHCCCCCCE
WAVAWKYPFAQALADVRKVHFKIGRTGRITPVLELSPVMLDDRQIKRVSVSSLRRWQELD
EEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHCC
IRPGDQVAISLAGLTIPRLDSVVLRSTERADLNVPLASDFHALSCWQPTPGCESQFLARL
CCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHH
TWLSGKQGLALPHVGRGTWEKLLETGRLNSLLDWLTLDGPELANIAGLGERSSARLLHSF
HHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHH
HSARQRPFAQWLKALGLPPTGQATLADSWQALAQRNTEQWQAEAGIGPGRAAQLSAFFRD
HHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCC
PQVLTLSETLQAAGVDGF
CCEEEHHHHHHHCCCCCC
>Mature Secondary Structure
MLATLRLFLFFLPFFHLHTFADDCPDWAAARASSEVVALQQQIDRWDDAYHREGRSVIAD
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
ELYDQSRLRLNQWRQCFNLPSPPEPLRTASGPVAHPVAHTGLDKLHDAADIATWLRDRQN
HHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCC
VWVQPKVDGVAVTLIYRDGRLHQAISRGDGVRGQDWTASAKKIGAIPQQLTQPQDLLVQG
EEEEECCCCEEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHCCCHHEEEEC
ELYWRLNGHMQARAGSANARATVAGLLGRKDLGAEHAAGIGLFVWDWPHGPRDLPERITA
EEEEEECCCEEECCCCCCCHHHHHHHHCCHHCCCHHHCCCEEEEEECCCCCHHHHHHHHH
LTQFGFPTTVPYTQAVASLNDAQHWRDHWYHSPLPFATDGVVLRQSLRPPAERWQARPPY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHCCCCCCE
WAVAWKYPFAQALADVRKVHFKIGRTGRITPVLELSPVMLDDRQIKRVSVSSLRRWQELD
EEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHCC
IRPGDQVAISLAGLTIPRLDSVVLRSTERADLNVPLASDFHALSCWQPTPGCESQFLARL
CCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHH
TWLSGKQGLALPHVGRGTWEKLLETGRLNSLLDWLTLDGPELANIAGLGERSSARLLHSF
HHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHH
HSARQRPFAQWLKALGLPPTGQATLADSWQALAQRNTEQWQAEAGIGPGRAAQLSAFFRD
HHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHCC
PQVLTLSETLQAAGVDGF
CCEEEHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA