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The map label for this gene is maeB [H]

Identifier: 74317199

GI number: 74317199

Start: 1241111

End: 1242334

Strand: Direct

Name: maeB [H]

Synonym: Tbd_1181

Alternate gene names: 74317199

Gene position: 1241111-1242334 (Clockwise)

Preceding gene: 74317198

Following gene: 74317200

Centisome position: 42.65

GC content: 64.95

Gene sequence:

>1224_bases
ATGAGCCAAGCCTCCCTCAAGAAAAAAGCCCTCGACTACCACCGCCTGCCCAGACCTGGCAAGCTCGAAGTCGTTTCGTC
GAAACCCTGCGCGACCCAGGCCGACCTCTCGCTCGCCTATACCCCGGGCGTCGCCCAGCCGGTGCTGGAGATCGCGAAGA
ATCCCGCCAAGGCCTACGACTACACCAACAAGGGCAATCTCGTCGCCGTGATCACCGACGGTACCGCGATTCTCGGACTC
GGTGACCGCGGCGCGCTCGCCGCGAAGCCGGTCATGGAAGGCAAGGCCGTCCTCTTCAAGCAATTCGCCGACATCGACGT
CTACGACATCGAGGTCAATGCCAGAACGCCGCAGGACTTCATCAAGGTCGTCGAGGCGATCGCGCCGACCTTCGGCGGCA
TCAATCTCGAGGACATCGCCGCACCGCACTGCTTCGAGATCGAAGCCGCGCTGAAGAAGAAGCTCGACATCCCGGTGTTC
CACGACGACCAGCATGGCACCGCCGTTATCATCTGCGCGGGGCTCATCAACGCGCTGCACGTCCAGGGCAAGACGCTCGA
CAGTGCGAAGATCGTCTGCCTCGGCGCGGGCGCCGCGGGGATCGCGTCGATGAAGCTCCTGCTCGCGATGGGCGCGAGGA
AGTCCAACATCCTGCTCGTCGATTCCAAGGGCGTCGTGCACAAGGGGCGCACCGACCTGAACGCCTTCAAGAAGGGTTTC
GCCCGCGCGACCAAACTGCGTACGCTCGAGGACGCGATGAACGGCGCCGACGTGTTCGTCGGCGTCTCGGGCGCCGACCT
CGTGAGCCCGGCCATGGTCAAGAGCATGGCCGACAACCCGGTGGTTTTCGCGCTCGCCAACCCGAATCCCGAGATCTCGC
CGGCGAAGGCGATGGCCGCGCGCAGCGACCTCGTGATGGCGACCGGACGCTCCGACTATCCGAACCAGGTCAACAACGTA
CTTGGCTTTCCCTTCATCTTCCGCGGCGCGCTCGATGCGCGCGCACGCGAGATCACGCAGGACATGCTGATCGCCGCCGT
CAACGCGCTCGCCGAACTCGCGCGCGAGCCGGTTCCGGCTGCGGTGCTCAAGGCCTATCAGCTGAAGAAACTCAAGTTCG
GACCGGACTACATCCTGCCCAAGCCTTTCGACCCGCGGCTGGCCGAGCGTGTGCCGCAGGCCGTCGCCAAGGCGGCCCGG
AAGGCCGTCAGAAAACGGCGCTGA

Upstream 100 bases:

>100_bases
GCGCAACTCAAGATCTGGAAAGACTGACCGCGGCCCGCGCCGACATGCGCGGTCAAATCCGCGATAATGGCGGCTTCGTC
AATCCGCTCGCCGTCTTTCC

Downstream 100 bases:

>100_bases
GCGAACAAAACACGGCCGTGAAGCCCGTCCCGCGCCCCGTTTATCTCGATCTGTTCCGCATCCATCTGCCCCTGCCGGGC
TGGGTGTCGATCCTGCACCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSQASLKKKALDYHRLPRPGKLEVVSSKPCATQADLSLAYTPGVAQPVLEIAKNPAKAYDYTNKGNLVAVITDGTAILGL
GDRGALAAKPVMEGKAVLFKQFADIDVYDIEVNARTPQDFIKVVEAIAPTFGGINLEDIAAPHCFEIEAALKKKLDIPVF
HDDQHGTAVIICAGLINALHVQGKTLDSAKIVCLGAGAAGIASMKLLLAMGARKSNILLVDSKGVVHKGRTDLNAFKKGF
ARATKLRTLEDAMNGADVFVGVSGADLVSPAMVKSMADNPVVFALANPNPEISPAKAMAARSDLVMATGRSDYPNQVNNV
LGFPFIFRGALDARAREITQDMLIAAVNALAELAREPVPAAVLKAYQLKKLKFGPDYILPKPFDPRLAERVPQAVAKAAR
KAVRKRR

Sequences:

>Translated_407_residues
MSQASLKKKALDYHRLPRPGKLEVVSSKPCATQADLSLAYTPGVAQPVLEIAKNPAKAYDYTNKGNLVAVITDGTAILGL
GDRGALAAKPVMEGKAVLFKQFADIDVYDIEVNARTPQDFIKVVEAIAPTFGGINLEDIAAPHCFEIEAALKKKLDIPVF
HDDQHGTAVIICAGLINALHVQGKTLDSAKIVCLGAGAAGIASMKLLLAMGARKSNILLVDSKGVVHKGRTDLNAFKKGF
ARATKLRTLEDAMNGADVFVGVSGADLVSPAMVKSMADNPVVFALANPNPEISPAKAMAARSDLVMATGRSDYPNQVNNV
LGFPFIFRGALDARAREITQDMLIAAVNALAELAREPVPAAVLKAYQLKKLKFGPDYILPKPFDPRLAERVPQAVAKAAR
KAVRKRR
>Mature_406_residues
SQASLKKKALDYHRLPRPGKLEVVSSKPCATQADLSLAYTPGVAQPVLEIAKNPAKAYDYTNKGNLVAVITDGTAILGLG
DRGALAAKPVMEGKAVLFKQFADIDVYDIEVNARTPQDFIKVVEAIAPTFGGINLEDIAAPHCFEIEAALKKKLDIPVFH
DDQHGTAVIICAGLINALHVQGKTLDSAKIVCLGAGAAGIASMKLLLAMGARKSNILLVDSKGVVHKGRTDLNAFKKGFA
RATKLRTLEDAMNGADVFVGVSGADLVSPAMVKSMADNPVVFALANPNPEISPAKAMAARSDLVMATGRSDYPNQVNNVL
GFPFIFRGALDARAREITQDMLIAAVNALAELAREPVPAAVLKAYQLKKLKFGPDYILPKPFDPRLAERVPQAVAKAARK
AVRKRR

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=346, Percent_Identity=27.4566473988439, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI62420882, Length=349, Percent_Identity=25.5014326647564, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI62420880, Length=349, Percent_Identity=25.5014326647564, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI239049447, Length=349, Percent_Identity=25.5014326647564, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4505145, Length=345, Percent_Identity=27.2463768115942, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=27.9874213836478, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788806, Length=400, Percent_Identity=57, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI87081919, Length=402, Percent_Identity=24.6268656716418, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646388, Length=345, Percent_Identity=28.695652173913, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24646386, Length=345, Percent_Identity=28.695652173913, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281363505, Length=397, Percent_Identity=25.6926952141058, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707236, Length=397, Percent_Identity=25.6926952141058, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707232, Length=397, Percent_Identity=25.6926952141058, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281363503, Length=397, Percent_Identity=25.6926952141058, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362672, Length=400, Percent_Identity=26, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356279, Length=400, Percent_Identity=26, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362674, Length=400, Percent_Identity=26, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI78707242, Length=342, Percent_Identity=26.6081871345029, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78707238, Length=342, Percent_Identity=26.6081871345029, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78707240, Length=342, Percent_Identity=26.6081871345029, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 43391; Mature: 43260

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQASLKKKALDYHRLPRPGKLEVVSSKPCATQADLSLAYTPGVAQPVLEIAKNPAKAYD
CCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHCCCHHHCC
YTNKGNLVAVITDGTAILGLGDRGALAAKPVMEGKAVLFKQFADIDVYDIEVNARTPQDF
CCCCCCEEEEEECCEEEEEECCCCCCCCCCHHCCHHHHHHHHCCCEEEEEEECCCCHHHH
IKVVEAIAPTFGGINLEDIAAPHCFEIEAALKKKLDIPVFHDDQHGTAVIICAGLINALH
HHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHH
VQGKTLDSAKIVCLGAGAAGIASMKLLLAMGARKSNILLVDSKGVVHKGRTDLNAFKKGF
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCHHHHHHHHH
ARATKLRTLEDAMNGADVFVGVSGADLVSPAMVKSMADNPVVFALANPNPEISPAKAMAA
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
RSDLVMATGRSDYPNQVNNVLGFPFIFRGALDARAREITQDMLIAAVNALAELAREPVPA
HCCEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVLKAYQLKKLKFGPDYILPKPFDPRLAERVPQAVAKAARKAVRKRR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQASLKKKALDYHRLPRPGKLEVVSSKPCATQADLSLAYTPGVAQPVLEIAKNPAKAYD
CCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHCCCHHHCC
YTNKGNLVAVITDGTAILGLGDRGALAAKPVMEGKAVLFKQFADIDVYDIEVNARTPQDF
CCCCCCEEEEEECCEEEEEECCCCCCCCCCHHCCHHHHHHHHCCCEEEEEEECCCCHHHH
IKVVEAIAPTFGGINLEDIAAPHCFEIEAALKKKLDIPVFHDDQHGTAVIICAGLINALH
HHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHH
VQGKTLDSAKIVCLGAGAAGIASMKLLLAMGARKSNILLVDSKGVVHKGRTDLNAFKKGF
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCHHHHHHHHH
ARATKLRTLEDAMNGADVFVGVSGADLVSPAMVKSMADNPVVFALANPNPEISPAKAMAA
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
RSDLVMATGRSDYPNQVNNVLGFPFIFRGALDARAREITQDMLIAAVNALAELAREPVPA
HCCEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVLKAYQLKKLKFGPDYILPKPFDPRLAERVPQAVAKAARKAVRKRR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]