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The map label for this gene is fliA [H]

Identifier: 74317198

GI number: 74317198

Start: 1240327

End: 1241037

Strand: Direct

Name: fliA [H]

Synonym: Tbd_1180

Alternate gene names: 74317198

Gene position: 1240327-1241037 (Clockwise)

Preceding gene: 74317197

Following gene: 74317199

Centisome position: 42.63

GC content: 62.45

Gene sequence:

>711_bases
ATGGTCGGTAAACCATCGTCGCAGGACGAGCAGATCACCAAATATGCACCGCTGGTCAAGCGCATCGCGTATCACATGAT
GGCGCGCCTGCCGGCCAGCGTCGAAGTCGACGACCTGATCCAGGTCGGGTTGATCGGCCTCATGGACGCCGTATCGCGCT
TCGACGGCACGCAGGGTGCGCAATTCGAGTCGTATGCGACGCAGCGCATCCGTGGCGCGATGCTCGACGAGTTGCGCGAG
GCGGACTGGCTGCCGCGCCACGTACGGCAGAAGTCACGGCAGATCGAAGCGGCGATCCACAAGCTCGAACAGCGGCACGG
CAAGGCGCCGAGCGAGTCGGAAATATCGGCCGAACTCGGCATTCCGATCGATCAGTACCAGTCGATGCTTGGCGACGTCA
AATGCTCGCAGCTGCTCTACTACGAGGATTTTTCCGACGAGGACAGCGCGAGTTTTCTCGAACGCTATCTCGTCGACGGC
AGCAACGATCCGCTCGCGGTGCTCGAGGACGAGGGCTTCCGCGCCAGCCTGATCGCAGCGATCCATCACCTGCCCGAGCG
CGAGCGCAGCATGATGGGCATGTACTACGAGCAGGACATGAACCTCAAGGAGATCGGCGCGGTGCTCGGCGTCTCCGAAT
CACGCGTATGCCAGCTGCACTCGCAGGCCGTCGCGCGGCTGCGCGCGCAACTCAAGATCTGGAAAGACTGA

Upstream 100 bases:

>100_bases
GTGGCTGCAGAGACTGACGCTTTTGCCGCGCTTGCCGTTAGAATGAGCGCGGAGGAGGGCCGTTTGACGGCTTTTTATAA
CAAAGGGGCAAACCCGAATC

Downstream 100 bases:

>100_bases
CCGCGGCCCGCGCCGACATGCGCGGTCAAATCCGCGATAATGGCGGCTTCGTCAATCCGCTCGCCGTCTTTCCATGAGCC
AAGCCTCCCTCAAGAAAAAA

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVGKPSSQDEQITKYAPLVKRIAYHMMARLPASVEVDDLIQVGLIGLMDAVSRFDGTQGAQFESYATQRIRGAMLDELRE
ADWLPRHVRQKSRQIEAAIHKLEQRHGKAPSESEISAELGIPIDQYQSMLGDVKCSQLLYYEDFSDEDSASFLERYLVDG
SNDPLAVLEDEGFRASLIAAIHHLPERERSMMGMYYEQDMNLKEIGAVLGVSESRVCQLHSQAVARLRAQLKIWKD

Sequences:

>Translated_236_residues
MVGKPSSQDEQITKYAPLVKRIAYHMMARLPASVEVDDLIQVGLIGLMDAVSRFDGTQGAQFESYATQRIRGAMLDELRE
ADWLPRHVRQKSRQIEAAIHKLEQRHGKAPSESEISAELGIPIDQYQSMLGDVKCSQLLYYEDFSDEDSASFLERYLVDG
SNDPLAVLEDEGFRASLIAAIHHLPERERSMMGMYYEQDMNLKEIGAVLGVSESRVCQLHSQAVARLRAQLKIWKD
>Mature_236_residues
MVGKPSSQDEQITKYAPLVKRIAYHMMARLPASVEVDDLIQVGLIGLMDAVSRFDGTQGAQFESYATQRIRGAMLDELRE
ADWLPRHVRQKSRQIEAAIHKLEQRHGKAPSESEISAELGIPIDQYQSMLGDVKCSQLLYYEDFSDEDSASFLERYLVDG
SNDPLAVLEDEGFRASLIAAIHHLPERERSMMGMYYEQDMNLKEIGAVLGVSESRVCQLHSQAVARLRAQLKIWKD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=220, Percent_Identity=49.5454545454545, Blast_Score=214, Evalue=3e-57,
Organism=Escherichia coli, GI1789448, Length=200, Percent_Identity=29, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789098, Length=240, Percent_Identity=25, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26700; Mature: 26700

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGKPSSQDEQITKYAPLVKRIAYHMMARLPASVEVDDLIQVGLIGLMDAVSRFDGTQGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
QFESYATQRIRGAMLDELREADWLPRHVRQKSRQIEAAIHKLEQRHGKAPSESEISAELG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
IPIDQYQSMLGDVKCSQLLYYEDFSDEDSASFLERYLVDGSNDPLAVLEDEGFRASLIAA
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHH
IHHLPERERSMMGMYYEQDMNLKEIGAVLGVSESRVCQLHSQAVARLRAQLKIWKD
HHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVGKPSSQDEQITKYAPLVKRIAYHMMARLPASVEVDDLIQVGLIGLMDAVSRFDGTQGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
QFESYATQRIRGAMLDELREADWLPRHVRQKSRQIEAAIHKLEQRHGKAPSESEISAELG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
IPIDQYQSMLGDVKCSQLLYYEDFSDEDSASFLERYLVDGSNDPLAVLEDEGFRASLIAA
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHH
IHHLPERERSMMGMYYEQDMNLKEIGAVLGVSESRVCQLHSQAVARLRAQLKIWKD
HHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]