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The map label for this gene is gsiB [H]

Identifier: 66045507

GI number: 66045507

Start: 2632280

End: 2633800

Strand: Reverse

Name: gsiB [H]

Synonym: Psyr_2267

Alternate gene names: 66045507

Gene position: 2633800-2632280 (Counterclockwise)

Preceding gene: 66045512

Following gene: 66045506

Centisome position: 43.22

GC content: 60.49

Gene sequence:

>1521_bases
ATGGGAATCAAGGGTTTCGCTCGCAGCATCGCGCTATTGAGCCTGTTCAGCAGTTTCTCCGTCCTGGCCGGCAAGGCTGA
CGACACACTGGTCTACGCCTCCGACAGCGAACCGGAAAACATCAGCCCCTATCACAACGACTTGCGCGAAGGCGTGATCC
TCGGTCGCCTGATCTGGGACAACCTGGTCTATCGCAACCCGGACAATGGCGAGTACCAACCGATGCTCGCCACCAGCTGG
AAACAGGTCGACGATACGACCATCGACTTCCAGCTGCGCCAGGGCGTGAAGTTTCACAACGGCGACCCGTTCACTGCAGA
TGACGTGGTGTTCACCCTCAATTATGTGGTGTCTCCCGAGTCCAAGGTGGTCACCGTCCAAAACGTCGACTGGATCAAGA
GCGCCGAAAAACTGGGCGATTACAGTGTTCGTCTGCACCTGAAAAAGCCCTTCCCGCCTGCGCTGGAATACCTCTCCAAC
GCCGTACCGATGTTTCCGAAAAAATATTTCGAAGAAGTCGGGCTGGCAGGCTTCAGCCGTAAACCGATCGGCACCGGGCC
TTACAAGGTGACGGCCATCGCCACCGGCGAAGGCGTGAAAATGGACAAGAACCCGGACTACTTCAAGGACAGCCCGCAAG
GTCAGCCAAAGATCGGGCACATCAACTTTCGGGTGATCGCCGATGCCGAAACGCGCCTGGCCGAGCTGATGACCGGTGGC
GTCGACTGGACCTGGCGGGTGGCGCCGGATCAGGCGGAAAACCTCAAGGCCATGCCCAACCTGGTGGTCACCAGCGGTGC
GACCATGCGCATCGGCTTTCTGATTCTCGACGCGCGCGGCACCTCCAGCGCCGATTCGCCGATGAAGCACCTGAAAGTAC
GTCAGGCGATCAACTACGCGATCAATCGGGACGGGCTGGCTTCGCAACTGGTCGGCGGCGAAAGCAAGCCGTTGCAAGTC
GCCTGCTACCCTGGCCAGTTCGGTTGTGACACCACCGCCGCCACGGTCTACAACTACGACCCGGCAAAGGCCAAGGCGCT
GCTCGCCGAAGCCGGCTACCCGAACGGCTTTGAAACCGAAATCTTCGCTTACCGCGACCGCGACTACGTTGAAGCCATCA
TCGGCAACCTGCGCGCCGTGGGCATCAACGCCAAGCTGCGCTACCTGAAATACGCCGCCCTGCGTGATCAGCAGCGCGGC
GGCAAGGTACCGATGTCGTTCCAGGCGTGGGGATCGTTCTCGATCCTCGACACCTCGGCGTCGGCCGGCACCTGGTTCAA
GGGCAACCCCGACGACAACATCAAGGACCCGCAAGTCCAGGGCTGGCTGCAGACCGCCGACAACGCGCTGGACCCGCAGG
TGCGCAAGGACAACTACCGCAAGGCGTTGCAGCGCATCAGCGAGCAGGCCTACTGGGCGCCGCTGTTCAACTACTCGATG
AACTACGCCTATGTCTCGGACCTGAACTTCAAGCCTTACCCGGACGAGCTGCCGCGCTTCGTACTGTCGAGCTGGAAATA
A

Upstream 100 bases:

>100_bases
ACCCAATCAAAAGAACACTGCGGCCTTGTTGCTTCTTCCTTCTCGCATTTGCGACCCACTGCCCTTTGATACATCCACTT
TGCCTTTTGGAGACGACGGC

Downstream 100 bases:

>100_bases
GCACTTGCCCAAGCCAGTCGGCCAGCCTGGCTGTAAGGCGCATGCCGCGCCTTGCAGCAGGGTCAGCCACGCCGTCATTC
CCTGAACACAAGGTCACTTG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MGIKGFARSIALLSLFSSFSVLAGKADDTLVYASDSEPENISPYHNDLREGVILGRLIWDNLVYRNPDNGEYQPMLATSW
KQVDDTTIDFQLRQGVKFHNGDPFTADDVVFTLNYVVSPESKVVTVQNVDWIKSAEKLGDYSVRLHLKKPFPPALEYLSN
AVPMFPKKYFEEVGLAGFSRKPIGTGPYKVTAIATGEGVKMDKNPDYFKDSPQGQPKIGHINFRVIADAETRLAELMTGG
VDWTWRVAPDQAENLKAMPNLVVTSGATMRIGFLILDARGTSSADSPMKHLKVRQAINYAINRDGLASQLVGGESKPLQV
ACYPGQFGCDTTAATVYNYDPAKAKALLAEAGYPNGFETEIFAYRDRDYVEAIIGNLRAVGINAKLRYLKYAALRDQQRG
GKVPMSFQAWGSFSILDTSASAGTWFKGNPDDNIKDPQVQGWLQTADNALDPQVRKDNYRKALQRISEQAYWAPLFNYSM
NYAYVSDLNFKPYPDELPRFVLSSWK

Sequences:

>Translated_506_residues
MGIKGFARSIALLSLFSSFSVLAGKADDTLVYASDSEPENISPYHNDLREGVILGRLIWDNLVYRNPDNGEYQPMLATSW
KQVDDTTIDFQLRQGVKFHNGDPFTADDVVFTLNYVVSPESKVVTVQNVDWIKSAEKLGDYSVRLHLKKPFPPALEYLSN
AVPMFPKKYFEEVGLAGFSRKPIGTGPYKVTAIATGEGVKMDKNPDYFKDSPQGQPKIGHINFRVIADAETRLAELMTGG
VDWTWRVAPDQAENLKAMPNLVVTSGATMRIGFLILDARGTSSADSPMKHLKVRQAINYAINRDGLASQLVGGESKPLQV
ACYPGQFGCDTTAATVYNYDPAKAKALLAEAGYPNGFETEIFAYRDRDYVEAIIGNLRAVGINAKLRYLKYAALRDQQRG
GKVPMSFQAWGSFSILDTSASAGTWFKGNPDDNIKDPQVQGWLQTADNALDPQVRKDNYRKALQRISEQAYWAPLFNYSM
NYAYVSDLNFKPYPDELPRFVLSSWK
>Mature_505_residues
GIKGFARSIALLSLFSSFSVLAGKADDTLVYASDSEPENISPYHNDLREGVILGRLIWDNLVYRNPDNGEYQPMLATSWK
QVDDTTIDFQLRQGVKFHNGDPFTADDVVFTLNYVVSPESKVVTVQNVDWIKSAEKLGDYSVRLHLKKPFPPALEYLSNA
VPMFPKKYFEEVGLAGFSRKPIGTGPYKVTAIATGEGVKMDKNPDYFKDSPQGQPKIGHINFRVIADAETRLAELMTGGV
DWTWRVAPDQAENLKAMPNLVVTSGATMRIGFLILDARGTSSADSPMKHLKVRQAINYAINRDGLASQLVGGESKPLQVA
CYPGQFGCDTTAATVYNYDPAKAKALLAEAGYPNGFETEIFAYRDRDYVEAIIGNLRAVGINAKLRYLKYAALRDQQRGG
KVPMSFQAWGSFSILDTSASAGTWFKGNPDDNIKDPQVQGWLQTADNALDPQVRKDNYRKALQRISEQAYWAPLFNYSMN
YAYVSDLNFKPYPDELPRFVLSSWK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=444, Percent_Identity=26.5765765765766, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789966, Length=440, Percent_Identity=26.1363636363636, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789887, Length=525, Percent_Identity=24.7619047619048, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787762, Length=500, Percent_Identity=23.8, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787551, Length=388, Percent_Identity=25.5154639175258, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87082063, Length=157, Percent_Identity=27.3885350318471, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56367; Mature: 56236

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKGFARSIALLSLFSSFSVLAGKADDTLVYASDSEPENISPYHNDLREGVILGRLIWD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
NLVYRNPDNGEYQPMLATSWKQVDDTTIDFQLRQGVKFHNGDPFTADDVVFTLNYVVSPE
HHEEECCCCCCCCCHHHCCCHHCCCCEEEEEECCCCEECCCCCCCCCCEEEEEEEEECCC
SKVVTVQNVDWIKSAEKLGDYSVRLHLKKPFPPALEYLSNAVPMFPKKYFEEVGLAGFSR
CCEEEEECCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCC
KPIGTGPYKVTAIATGEGVKMDKNPDYFKDSPQGQPKIGHINFRVIADAETRLAELMTGG
CCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHCC
VDWTWRVAPDQAENLKAMPNLVVTSGATMRIGFLILDARGTSSADSPMKHLKVRQAINYA
CCEEEEECCCHHHCHHHCCCEEEECCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
INRDGLASQLVGGESKPLQVACYPGQFGCDTTAATVYNYDPAKAKALLAEAGYPNGFETE
CCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCE
IFAYRDRDYVEAIIGNLRAVGINAKLRYLKYAALRDQQRGGKVPMSFQAWGSFSILDTSA
EEEECCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCC
SAGTWFKGNPDDNIKDPQVQGWLQTADNALDPQVRKDNYRKALQRISEQAYWAPLFNYSM
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHCCC
NYAYVSDLNFKPYPDELPRFVLSSWK
CEEEEECCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
GIKGFARSIALLSLFSSFSVLAGKADDTLVYASDSEPENISPYHNDLREGVILGRLIWD
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
NLVYRNPDNGEYQPMLATSWKQVDDTTIDFQLRQGVKFHNGDPFTADDVVFTLNYVVSPE
HHEEECCCCCCCCCHHHCCCHHCCCCEEEEEECCCCEECCCCCCCCCCEEEEEEEEECCC
SKVVTVQNVDWIKSAEKLGDYSVRLHLKKPFPPALEYLSNAVPMFPKKYFEEVGLAGFSR
CCEEEEECCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCCC
KPIGTGPYKVTAIATGEGVKMDKNPDYFKDSPQGQPKIGHINFRVIADAETRLAELMTGG
CCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHCC
VDWTWRVAPDQAENLKAMPNLVVTSGATMRIGFLILDARGTSSADSPMKHLKVRQAINYA
CCEEEEECCCHHHCHHHCCCEEEECCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
INRDGLASQLVGGESKPLQVACYPGQFGCDTTAATVYNYDPAKAKALLAEAGYPNGFETE
CCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCE
IFAYRDRDYVEAIIGNLRAVGINAKLRYLKYAALRDQQRGGKVPMSFQAWGSFSILDTSA
EEEECCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCC
SAGTWFKGNPDDNIKDPQVQGWLQTADNALDPQVRKDNYRKALQRISEQAYWAPLFNYSM
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHCCC
NYAYVSDLNFKPYPDELPRFVLSSWK
CEEEEECCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA