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The map label for this gene is trpE [H]

Identifier: 59711640

GI number: 59711640

Start: 1136729

End: 1137604

Strand: Reverse

Name: trpE [H]

Synonym: VF_1033

Alternate gene names: 59711640

Gene position: 1137604-1136729 (Counterclockwise)

Preceding gene: 172087686

Following gene: 59711639

Centisome position: 39.26

GC content: 43.84

Gene sequence:

>876_bases
ATGCAGTTCCAAGTGTCTCTGATGATGATTTGCCAAACGGTTTTAAATTTAAAAGAATTTGTCATCAAAGGAGATGTGTT
CCAAGTTGTTCCTTCTCGTCGTTTTACTCTGCCTTGCCCTGCTCCACTTTCCGCTTACCGCGAACTGAAGATCGGTAACC
CAAGTCCTTATATGTTCTATCTGCAAGATCGTGATTTCACACTGTTTGGAGCCTCTCCTGAATCAGCTCTTAAATATTGC
AAAGTCAGCAATCAAGTTGAAATTTATCCAATAGCAGGTACTCGTAAACGTGGTAAAAATGCAGATGGTTCCATCAACCA
CGATTTAGATAGTCGTATCGAATTGGAATTACGCTGTGACACAAAAGAAAATGCAGAACACATGATGCTGGTTGATTTAG
CACGAAACGATGTAGCTCGAATTTCGCAAGCTGGCACTCGTCACGTTGCTGATTTGCTTAAAGTGGATCGCTATAGTCAT
GTTATGCATTTAGTGTCACGAGTGGTTGGGCAACTCAGAACCGAACTCGATGCTCTTCACGCTTATCAAGCATGCATGAA
CATGGGAACCCTCACTGGTGCTCCTAAAATTCGAGCAATGCAACTTATCCGTGATGTAGAAAAAACTCGCCGTGGTGCTT
ATGGTGGCGCTGTGGGTTATTTAACGGGAGATGGCAGCATGGATACCTGTATTGTGATCCGTTCAGCTTATGTCGAAGAT
AGTGTTGCTCAAGTTCAAGCTGGTGCCGGCGTAGTATATGACTCAGATCCTCAGGCAGAAGCTGATGAAACACGAGGCAA
AGCACAAGCGGTTATCTCAGCCATTCAAAAAGCACACTTATCGGCAACCGCGCAACAAGTTAAGGAGACAAAATAA

Upstream 100 bases:

>100_bases
GAAGAAATAAAGCAACAAGTATCAAAACGTTTGTCTTCCATAAAAACATTCTGCTCTGAATCTCATGATGTTCCATTAGC
GCCAGTCTTAACTGAATGTG

Downstream 100 bases:

>100_bases
TGGCTCACATTGTATTACTCGATAACTTTGATTCCTTTACCTACAACTTAGTTGATCAATTTCGCTCTCTTGGTTACCCA
GTGACGATTTACCGTAATAG

Product: component I of anthranilate synthase

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQFQVSLMMICQTVLNLKEFVIKGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFYLQDRDFTLFGASPESALKYC
KVSNQVEIYPIAGTRKRGKNADGSINHDLDSRIELELRCDTKENAEHMMLVDLARNDVARISQAGTRHVADLLKVDRYSH
VMHLVSRVVGQLRTELDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGAYGGAVGYLTGDGSMDTCIVIRSAYVED
SVAQVQAGAGVVYDSDPQAEADETRGKAQAVISAIQKAHLSATAQQVKETK

Sequences:

>Translated_291_residues
MQFQVSLMMICQTVLNLKEFVIKGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFYLQDRDFTLFGASPESALKYC
KVSNQVEIYPIAGTRKRGKNADGSINHDLDSRIELELRCDTKENAEHMMLVDLARNDVARISQAGTRHVADLLKVDRYSH
VMHLVSRVVGQLRTELDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGAYGGAVGYLTGDGSMDTCIVIRSAYVED
SVAQVQAGAGVVYDSDPQAEADETRGKAQAVISAIQKAHLSATAQQVKETK
>Mature_291_residues
MQFQVSLMMICQTVLNLKEFVIKGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFYLQDRDFTLFGASPESALKYC
KVSNQVEIYPIAGTRKRGKNADGSINHDLDSRIELELRCDTKENAEHMMLVDLARNDVARISQAGTRHVADLLKVDRYSH
VMHLVSRVVGQLRTELDALHAYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGAYGGAVGYLTGDGSMDTCIVIRSAYVED
SVAQVQAGAGVVYDSDPQAEADETRGKAQAVISAIQKAHLSATAQQVKETK

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=263, Percent_Identity=71.4828897338403, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1788114, Length=262, Percent_Identity=33.206106870229, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786809, Length=215, Percent_Identity=29.7674418604651, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082077, Length=262, Percent_Identity=27.8625954198473, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=267, Percent_Identity=45.6928838951311, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324361, Length=267, Percent_Identity=26.5917602996255, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 32146; Mature: 32146

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFQVSLMMICQTVLNLKEFVIKGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFY
CCEEHHHHHHHHHHHHHHHHHHCCCHHEECCCCEEECCCCCCHHHHHEEECCCCCCEEEE
LQDRDFTLFGASPESALKYCKVSNQVEIYPIAGTRKRGKNADGSINHDLDSRIELELRCD
EECCCEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
TKENAEHMMLVDLARNDVARISQAGTRHVADLLKVDRYSHVMHLVSRVVGQLRTELDALH
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGAYGGAVGYLTGDGSMDTCIVIRSAYVED
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEHHHHHHH
SVAQVQAGAGVVYDSDPQAEADETRGKAQAVISAIQKAHLSATAQQVKETK
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFQVSLMMICQTVLNLKEFVIKGDVFQVVPSRRFTLPCPAPLSAYRELKIGNPSPYMFY
CCEEHHHHHHHHHHHHHHHHHHCCCHHEECCCCEEECCCCCCHHHHHEEECCCCCCEEEE
LQDRDFTLFGASPESALKYCKVSNQVEIYPIAGTRKRGKNADGSINHDLDSRIELELRCD
EECCCEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
TKENAEHMMLVDLARNDVARISQAGTRHVADLLKVDRYSHVMHLVSRVVGQLRTELDALH
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYQACMNMGTLTGAPKIRAMQLIRDVEKTRRGAYGGAVGYLTGDGSMDTCIVIRSAYVED
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEHHHHHHH
SVAQVQAGAGVVYDSDPQAEADETRGKAQAVISAIQKAHLSATAQQVKETK
HHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]