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The map label for this gene is trpD [H]

Identifier: 59711639

GI number: 59711639

Start: 1136127

End: 1136729

Strand: Reverse

Name: trpD [H]

Synonym: VF_1032

Alternate gene names: 59711639

Gene position: 1136729-1136127 (Counterclockwise)

Preceding gene: 59711640

Following gene: 172087685

Centisome position: 39.23

GC content: 41.46

Gene sequence:

>603_bases
ATGGCTCACATTGTATTACTCGATAACTTTGATTCCTTTACCTACAACTTAGTTGATCAATTTCGCTCTCTTGGTTACCC
AGTGACGATTTACCGTAATAGCTTAACCGCAGCCGCTTTTGAAGAAGCCGTAAATAAGCTAGACAATCCAGTTATCGTTT
TATCTCCTGGCCCTGGAGCACCATCAGAAGCAGGTTGTATGCCTGAAGTTATTCAGCGCCTAAAAGGCAAAATCCCAATG
ATAGGTATTTGTCTTGGGCACCAAGCGATTGTTGAGGCCTATAACGGTACGGTTGCCGGAGCAGGTGAAATTGTTCATGG
TAAATCAGCAATGATGAACCATAACCAACACCCTATTTTTAATGACTTACCAAATCCACTCTCTATTGCTCGTTACCACT
CATTAGTGGCAACAAAAGTAACGAATGAACTAACCATTACAGCCACAGTAGATGACCTTGTAATGGCCGTAATTCAAGAA
GAAGACAAGGTGTGTGGCTTCCAATTTCACCCAGAATCCATTTTGACGACTCAAGGGGCACAACTGCTCATTAATACCTT
GAATTGGGCATTAGATCTAACAGCAAAAGACAATAAAGAATAA

Upstream 100 bases:

>100_bases
CAGAAGCTGATGAAACACGAGGCAAAGCACAAGCGGTTATCTCAGCCATTCAAAAAGCACACTTATCGGCAACCGCGCAA
CAAGTTAAGGAGACAAAATA

Downstream 100 bases:

>100_bases
GGATATTCTCATGACTCAAATAGAAACCATTTTTAACAAGCTATACGATCAAGTTTCTCTTTCAGAATCAGAAAGCCAAG
TTCTTTTTGATGCCATTATT

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MAHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLTAAAFEEAVNKLDNPVIVLSPGPGAPSEAGCMPEVIQRLKGKIPM
IGICLGHQAIVEAYNGTVAGAGEIVHGKSAMMNHNQHPIFNDLPNPLSIARYHSLVATKVTNELTITATVDDLVMAVIQE
EDKVCGFQFHPESILTTQGAQLLINTLNWALDLTAKDNKE

Sequences:

>Translated_200_residues
MAHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLTAAAFEEAVNKLDNPVIVLSPGPGAPSEAGCMPEVIQRLKGKIPM
IGICLGHQAIVEAYNGTVAGAGEIVHGKSAMMNHNQHPIFNDLPNPLSIARYHSLVATKVTNELTITATVDDLVMAVIQE
EDKVCGFQFHPESILTTQGAQLLINTLNWALDLTAKDNKE
>Mature_199_residues
AHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLTAAAFEEAVNKLDNPVIVLSPGPGAPSEAGCMPEVIQRLKGKIPMI
GICLGHQAIVEAYNGTVAGAGEIVHGKSAMMNHNQHPIFNDLPNPLSIARYHSLVATKVTNELTITATVDDLVMAVIQEE
DKVCGFQFHPESILTTQGAQLLINTLNWALDLTAKDNKE

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=190, Percent_Identity=61.5789473684211, Blast_Score=249, Evalue=8e-68,
Organism=Escherichia coli, GI1789760, Length=189, Percent_Identity=42.3280423280423, Blast_Score=148, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322638, Length=190, Percent_Identity=38.9473684210526, Blast_Score=145, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324361, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 21756; Mature: 21625

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLTAAAFEEAVNKLDNPVIVLSPGPGA
CEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCC
PSEAGCMPEVIQRLKGKIPMIGICLGHQAIVEAYNGTVAGAGEIVHGKSAMMNHNQHPIF
CCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCCCEEECCHHHHCCCCCCCC
NDLPNPLSIARYHSLVATKVTNELTITATVDDLVMAVIQEEDKVCGFQFHPESILTTQGA
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCEEECCCHHHHCCCH
QLLINTLNWALDLTAKDNKE
HHHHHHHHHEEEECCCCCCC
>Mature Secondary Structure 
AHIVLLDNFDSFTYNLVDQFRSLGYPVTIYRNSLTAAAFEEAVNKLDNPVIVLSPGPGA
EEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCC
PSEAGCMPEVIQRLKGKIPMIGICLGHQAIVEAYNGTVAGAGEIVHGKSAMMNHNQHPIF
CCCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCCCEEECCHHHHCCCCCCCC
NDLPNPLSIARYHSLVATKVTNELTITATVDDLVMAVIQEEDKVCGFQFHPESILTTQGA
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCEEECCCHHHHCCCH
QLLINTLNWALDLTAKDNKE
HHHHHHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]