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The map label for this gene is aroA [H]

Identifier: 57237724

GI number: 57237724

Start: 913014

End: 914300

Strand: Reverse

Name: aroA [H]

Synonym: CJE0974

Alternate gene names: 57237724

Gene position: 914300-913014 (Counterclockwise)

Preceding gene: 57237725

Following gene: 57237723

Centisome position: 51.43

GC content: 31.08

Gene sequence:

>1287_bases
ATGAAAATTTACAAATTGCAAACCCCTGTAAATGCTATACTTGAAAATATAGCAGCAGATAAAAGCATATCTCATCGTTT
TGCTATATTTTCGCTTTTAACACAAGAAGAAAATAAGGCTCAAAATTATCTCTTAGCTCAAGATACTTTAAACACTCTTG
AAATTATAAAAAATCTTGGAGCTAAAATTGAACAAAAAGATTCTTGCGTCAAAATTATACCCCCTAAAGAAATTTTATCT
CCAAATTGTATTTTAGACTGTGGAAATTCAGGAACTGCTATGCGTTTGATGATAGGATTTTTAGCAGGAATTTTTGGTTT
TTTTGTTTTAAGTGGAGATAAGTATTTAAACAATCGTCCTATGAGAAGGATAAGCAAACCACTTACTCAAATAGGCGCTA
GAATTTATGGAAGAAATGAGGCAAATTTAGCTCCACTTTGTATAGAAGGTCAAAAATTAAAAGCTTTTAATTTTAAAAGC
GAAATTTCTTCGGCTCAAGTTAAAACAGCTATGATTTTATCTGCTTTTAGAGCTGATAATGTATGCACTTTTAGTGAAAT
TTCTCTTAGTCGAAATCATAGTGAAAACATGCTAAAGGCTATGAAAGCTCCAATAAGGGTTAGTAATGATGGCTTAAGTC
TTGAAATAAATCCTTTAAAAAAACCTTTAAAAGCTCAAAATATAATCATTCCTAATGATCCTTCTTCGGCTTTTTATTTT
GTTTTAGCAGCTATTATTTTACCTAAATCTCAAATTATTTTAAAAAATATTTTGCTTAATCCTACTCGTATAGAGGCGTA
TAAAATTTTGCAAAAAATGGGTGCCAAACTTGAAATGACAATAACTCAAAATGATTTTGAAACTATTGGTGAGATCAGGG
TGGAGTCTAGCAAGCTTAATGGCATAGAAGTTAAAGATAATATCGCTTGGTTAATAGATGAAGCGCCTGCTTTGGCTATA
GCTTTTGCTTTGGCTAAGGGTAAATCTAGTTTAATAAATGCTAAAGAATTACGCGTTAAAGAAAGCGATAGGATTGCTGT
GATGGTTGAAAATCTAAAGCTTTGTGGTGTTGAAGCTAGAGAACTTGATGATGGTTTTGAAATAGAAGGTGGATGCGAAC
TAAAATCTTCAAAAATTAAAAGCTATGGAGATCACCGTATTGCTATGAGTTTTGCTATTTTAGGTTTGCTTTGTGGAATC
GAGATTGATGATAGTGATTGTATAAAAACTTCTTTTCCAAATTTTATAGAGATTTTATCAAATTTAGGAGCTAGGATTGA
TTATTGA

Upstream 100 bases:

>100_bases
TTTCTTTTAGGGATATAAATAAAACTCTAGAAGATAATCAAGTGAATGAATGTATGGATAAAATTTTAAATACTCTTAAA
AATTTGGGTCTTGATTTAAG

Downstream 100 bases:

>100_bases
ATTAGCAAAAAACTATGGTTTTTGTTTTGGTGTTAAAAGAGCTATTAAAAAAGCAGAACAAATAAAAGATGCTGCAACTA
TAGGCCCTCTTATTCACAAT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKIYKLQTPVNAILENIAADKSISHRFAIFSLLTQEENKAQNYLLAQDTLNTLEIIKNLGAKIEQKDSCVKIIPPKEILS
PNCILDCGNSGTAMRLMIGFLAGIFGFFVLSGDKYLNNRPMRRISKPLTQIGARIYGRNEANLAPLCIEGQKLKAFNFKS
EISSAQVKTAMILSAFRADNVCTFSEISLSRNHSENMLKAMKAPIRVSNDGLSLEINPLKKPLKAQNIIIPNDPSSAFYF
VLAAIILPKSQIILKNILLNPTRIEAYKILQKMGAKLEMTITQNDFETIGEIRVESSKLNGIEVKDNIAWLIDEAPALAI
AFALAKGKSSLINAKELRVKESDRIAVMVENLKLCGVEARELDDGFEIEGGCELKSSKIKSYGDHRIAMSFAILGLLCGI
EIDDSDCIKTSFPNFIEILSNLGARIDY

Sequences:

>Translated_428_residues
MKIYKLQTPVNAILENIAADKSISHRFAIFSLLTQEENKAQNYLLAQDTLNTLEIIKNLGAKIEQKDSCVKIIPPKEILS
PNCILDCGNSGTAMRLMIGFLAGIFGFFVLSGDKYLNNRPMRRISKPLTQIGARIYGRNEANLAPLCIEGQKLKAFNFKS
EISSAQVKTAMILSAFRADNVCTFSEISLSRNHSENMLKAMKAPIRVSNDGLSLEINPLKKPLKAQNIIIPNDPSSAFYF
VLAAIILPKSQIILKNILLNPTRIEAYKILQKMGAKLEMTITQNDFETIGEIRVESSKLNGIEVKDNIAWLIDEAPALAI
AFALAKGKSSLINAKELRVKESDRIAVMVENLKLCGVEARELDDGFEIEGGCELKSSKIKSYGDHRIAMSFAILGLLCGI
EIDDSDCIKTSFPNFIEILSNLGARIDY
>Mature_428_residues
MKIYKLQTPVNAILENIAADKSISHRFAIFSLLTQEENKAQNYLLAQDTLNTLEIIKNLGAKIEQKDSCVKIIPPKEILS
PNCILDCGNSGTAMRLMIGFLAGIFGFFVLSGDKYLNNRPMRRISKPLTQIGARIYGRNEANLAPLCIEGQKLKAFNFKS
EISSAQVKTAMILSAFRADNVCTFSEISLSRNHSENMLKAMKAPIRVSNDGLSLEINPLKKPLKAQNIIIPNDPSSAFYF
VLAAIILPKSQIILKNILLNPTRIEAYKILQKMGAKLEMTITQNDFETIGEIRVESSKLNGIEVKDNIAWLIDEAPALAI
AFALAKGKSSLINAKELRVKESDRIAVMVENLKLCGVEARELDDGFEIEGGCELKSSKIKSYGDHRIAMSFAILGLLCGI
EIDDSDCIKTSFPNFIEILSNLGARIDY

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=421, Percent_Identity=28.0285035629454, Blast_Score=104, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320332, Length=459, Percent_Identity=23.3115468409586, Blast_Score=83, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 47349; Mature: 47349

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYKLQTPVNAILENIAADKSISHRFAIFSLLTQEENKAQNYLLAQDTLNTLEIIKNLG
CEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCEEEEHHHHHHHHHHHHHC
AKIEQKDSCVKIIPPKEILSPNCILDCGNSGTAMRLMIGFLAGIFGFFVLSGDKYLNNRP
CCCCCCCCEEEECCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
MRRISKPLTQIGARIYGRNEANLAPLCIEGQKLKAFNFKSEISSAQVKTAMILSAFRADN
HHHHHHHHHHHHHHEECCCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
VCTFSEISLSRNHSENMLKAMKAPIRVSNDGLSLEINPLKKPLKAQNIIIPNDPSSAFYF
CEEEHHHHCCCCCHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCCCEEECCCCCHHHHH
VLAAIILPKSQIILKNILLNPTRIEAYKILQKMGAKLEMTITQNDFETIGEIRVESSKLN
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHEEEECCCCC
GIEVKDNIAWLIDEAPALAIAFALAKGKSSLINAKELRVKESDRIAVMVENLKLCGVEAR
CEEEECCEEEEECCCCHHHHHHHHHCCCHHHCCHHHHEECCCCCEEEEEECHHCCCCCHH
ELDDGFEIEGGCELKSSKIKSYGDHRIAMSFAILGLLCGIEIDDSDCIKTSFPNFIEILS
HCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHH
NLGARIDY
HCCCCCCC
>Mature Secondary Structure
MKIYKLQTPVNAILENIAADKSISHRFAIFSLLTQEENKAQNYLLAQDTLNTLEIIKNLG
CEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHCEEEEHHHHHHHHHHHHHC
AKIEQKDSCVKIIPPKEILSPNCILDCGNSGTAMRLMIGFLAGIFGFFVLSGDKYLNNRP
CCCCCCCCEEEECCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
MRRISKPLTQIGARIYGRNEANLAPLCIEGQKLKAFNFKSEISSAQVKTAMILSAFRADN
HHHHHHHHHHHHHHEECCCCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCC
VCTFSEISLSRNHSENMLKAMKAPIRVSNDGLSLEINPLKKPLKAQNIIIPNDPSSAFYF
CEEEHHHHCCCCCHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCCCEEECCCCCHHHHH
VLAAIILPKSQIILKNILLNPTRIEAYKILQKMGAKLEMTITQNDFETIGEIRVESSKLN
HHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCHHHHHHEEEECCCCC
GIEVKDNIAWLIDEAPALAIAFALAKGKSSLINAKELRVKESDRIAVMVENLKLCGVEAR
CEEEECCEEEEECCCCHHHHHHHHHCCCHHHCCHHHHEECCCCCEEEEEECHHCCCCCHH
ELDDGFEIEGGCELKSSKIKSYGDHRIAMSFAILGLLCGIEIDDSDCIKTSFPNFIEILS
HCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHH
NLGARIDY
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8973316 [H]