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The map label for this gene is ispH

Identifier: 57237723

GI number: 57237723

Start: 912191

End: 913024

Strand: Reverse

Name: ispH

Synonym: CJE0973

Alternate gene names: 57237723

Gene position: 913024-912191 (Counterclockwise)

Preceding gene: 57237724

Following gene: 57237722

Centisome position: 51.36

GC content: 28.9

Gene sequence:

>834_bases
TTGATTATTGAATTAGCAAAAAACTATGGTTTTTGTTTTGGTGTTAAAAGAGCTATTAAAAAAGCAGAACAAATAAAAGA
TGCTGCAACTATAGGCCCTCTTATTCACAATAACGAAGAAATTTCTCGTTTGCAAAAAAATTTTAATGTCAAAACTTTGG
AAAATATACAAGCTTTAAGTAATGAAAAAAAGGCTATTATAAGAACTCATGGTATTACAAAGCAAGATTTAGAAGAATTG
AGAAAAAAAGATATAGAAATTTTTGATGCCACTTGTCCTTTTGTTACAAAACCACAACAAATTTGCGAGCAAATGAGTAA
GGAGGGTTATGAAGTTGTTATTTTTGGAGATGAAAATCATCCCGAAGTTAAAGGAGTCAAAAGTTATGTAAGTACAAAAG
CTTATGTAGTTTTGGATAAAAAAGAACTTCAAAATATAAAACTTCCTAATAAAATAGCTGTAGTAAGCCAAACAACTAAG
AAGCCTGAGCATTTTATGGAGATAGTAAATTTTTTAATACTTAAAACCAAAGAAGTAAGAGTTTTTAACACTATATGTGA
TGCAACCTTTAAAAATCAAGATGCCATTAAAGAACTCTCGCTTAAAAGTGATGTTATGGTTGTTGTAGGGGGTAAAAACT
CAGCCAATACAAAACAACTTTTTTTGATTGCAAAAACCAATTGTGAAGATAGCTATCTAATTGAAACCGAAGAAGAGTTA
AAAAAAGAATGGTTTTTAGATAAAAAACATTGTGGCATTAGTGCAGGTGCTAGTACTCCTGATTGGATTATACAAAAAGT
TATCGCCAAAATAGAAAATTTTAAAATTAATTAA

Upstream 100 bases:

>100_bases
ATTTTAGGTTTGCTTTGTGGAATCGAGATTGATGATAGTGATTGTATAAAAACTTCTTTTCCAAATTTTATAGAGATTTT
ATCAAATTTAGGAGCTAGGA

Downstream 100 bases:

>100_bases
AAAATGTTTTTAAATAGAATAAAAAAGTCAAAAATTTAAAAAAAATTAACAAAAATAAAAAAACTTAACACTAATTAAAG
AAAAATAAGGTAAAATCAAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MIIELAKNYGFCFGVKRAIKKAEQIKDAATIGPLIHNNEEISRLQKNFNVKTLENIQALSNEKKAIIRTHGITKQDLEEL
RKKDIEIFDATCPFVTKPQQICEQMSKEGYEVVIFGDENHPEVKGVKSYVSTKAYVVLDKKELQNIKLPNKIAVVSQTTK
KPEHFMEIVNFLILKTKEVRVFNTICDATFKNQDAIKELSLKSDVMVVVGGKNSANTKQLFLIAKTNCEDSYLIETEEEL
KKEWFLDKKHCGISAGASTPDWIIQKVIAKIENFKIN

Sequences:

>Translated_277_residues
MIIELAKNYGFCFGVKRAIKKAEQIKDAATIGPLIHNNEEISRLQKNFNVKTLENIQALSNEKKAIIRTHGITKQDLEEL
RKKDIEIFDATCPFVTKPQQICEQMSKEGYEVVIFGDENHPEVKGVKSYVSTKAYVVLDKKELQNIKLPNKIAVVSQTTK
KPEHFMEIVNFLILKTKEVRVFNTICDATFKNQDAIKELSLKSDVMVVVGGKNSANTKQLFLIAKTNCEDSYLIETEEEL
KKEWFLDKKHCGISAGASTPDWIIQKVIAKIENFKIN
>Mature_277_residues
MIIELAKNYGFCFGVKRAIKKAEQIKDAATIGPLIHNNEEISRLQKNFNVKTLENIQALSNEKKAIIRTHGITKQDLEEL
RKKDIEIFDATCPFVTKPQQICEQMSKEGYEVVIFGDENHPEVKGVKSYVSTKAYVVLDKKELQNIKLPNKIAVVSQTTK
KPEHFMEIVNFLILKTKEVRVFNTICDATFKNQDAIKELSLKSDVMVVVGGKNSANTKQLFLIAKTNCEDSYLIETEEEL
KKEWFLDKKHCGISAGASTPDWIIQKVIAKIENFKIN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=291, Percent_Identity=29.2096219931272, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_CAMJ8 (A8FLU3)

Other databases:

- EMBL:   X89371
- EMBL:   CP000814
- PIR:   PC4273
- RefSeq:   YP_001482407.1
- STRING:   A8FLU3
- GeneID:   5616971
- GenomeReviews:   CP000814_GR
- KEGG:   cju:C8J_0831
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   CJEJ407148:C8J_0831-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 31523; Mature: 31523

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIELAKNYGFCFGVKRAIKKAEQIKDAATIGPLIHNNEEISRLQKNFNVKTLENIQALS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHCCCCHHHHHHHHHC
NEKKAIIRTHGITKQDLEELRKKDIEIFDATCPFVTKPQQICEQMSKEGYEVVIFGDENH
CCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
PEVKGVKSYVSTKAYVVLDKKELQNIKLPNKIAVVSQTTKKPEHFMEIVNFLILKTKEVR
CHHHHHHHHHCCEEEEEEEHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHH
VFNTICDATFKNQDAIKELSLKSDVMVVVGGKNSANTKQLFLIAKTNCEDSYLIETEEEL
HHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCEEECCHHHH
KKEWFLDKKHCGISAGASTPDWIIQKVIAKIENFKIN
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIIELAKNYGFCFGVKRAIKKAEQIKDAATIGPLIHNNEEISRLQKNFNVKTLENIQALS
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHCCCCHHHHHHHHHC
NEKKAIIRTHGITKQDLEELRKKDIEIFDATCPFVTKPQQICEQMSKEGYEVVIFGDENH
CCCCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
PEVKGVKSYVSTKAYVVLDKKELQNIKLPNKIAVVSQTTKKPEHFMEIVNFLILKTKEVR
CHHHHHHHHHCCEEEEEEEHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHH
VFNTICDATFKNQDAIKELSLKSDVMVVVGGKNSANTKQLFLIAKTNCEDSYLIETEEEL
HHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCEEECCHHHH
KKEWFLDKKHCGISAGASTPDWIIQKVIAKIENFKIN
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA