Click here to switch to the map view.

The map label for this gene is pheT [H]

Identifier: 57237725

GI number: 57237725

Start: 914297

End: 916618

Strand: Reverse

Name: pheT [H]

Synonym: CJE0975

Alternate gene names: 57237725

Gene position: 916618-914297 (Counterclockwise)

Preceding gene: 57237726

Following gene: 57237724

Centisome position: 51.56

GC content: 28.38

Gene sequence:

>2322_bases
ATGATAATTACTAAAAGTTGGTTGAATGATTGGTTAGAACTTGAAGAAATAAGTTCAGATAAAATAGCTAAAACTTTAAA
CTCTATAGGTATAGAAGTTGATAGGGTTGGCGCTTTAAAGGCTCCAGATAAAGTTGTTGTAGGCTATGTTAAGGAAAAAA
TTAAACATGAAAATTCAGATAAATTAAGCATTTGTCAAGTTGATATCGGAAGTGAAACCTTGCAAATTGTCTGCGGTGCT
GCGAATGTTGATGCAGGACAATTTGTAGCTGTTGCAACTAAAGGTGCTATCATGCCAAACGGTATGGAAATAAAAGAAGC
AAAATTGCGAGGAGTGGATTCTTGTGGTATGCTTTGTTCTAGTTTAGAACTGGGTTTTGAAAAAATCAATGAAGGAATTA
TGCTTTTAGATGAGAGTATAGGGAAATTAGAACTTGGGAGACCTTTAAATACTTATGAAATTTTTAATGATGAGCTAATA
GAAGTGGAACTTACTCCTAATAGGGGTGATTGTTTAAGTATTTATGGTATAGCTCGTGATTTAGCCGCAGCTTTAAATTT
AAATTTAAAAGAACCAAAACCATTTAAAGAAAGTGAAAATGTTTTAGGTATCGGTAGAATTTTGCGCTTAGCAGCTGAGA
AAGAACTTAATGGGCTTTATAATTATCGAGCCATAGGGCTAAAAGAAGAAATTCAAACAAATTTATTGCTTAGTTTAAGA
CTTGCTCAAATTGAAGGTTTGGGAAAAAATTCCATAGAAAATTTATTAAATTATGCAACGCATTCAACTGGTGTTCTTTT
TAATGCTTATGATTTAAGTAGTTTTTCTGAAAAAGATGAAGAATTTATCATTAATTTAAGCAAGCAAGTGCATGGAGAAA
CAAAGGTATCTTACAAAGATAAACTTTTAAGCTTTAGCGGAATTTTCCAAAATAATGAAAGTAGATGTAAAGATGATTCT
AAAATAATTATTATTGAGGCTAATTATACCGATCCATTGGTAATTGCAGATGCTAAAATATATCATAAAGATCAAGATGA
AAAAATGCTTTATAGAAGTTTTAGGGGAAGTGAACCTAAATTAAATTTAGGAATGGATTTTTTGCTTGGTATTTTTGAGC
ATATTCCAAATCTTGTTATTTATAGTTCTTCTCAACAAATTTTAACCGACAAAGAATTACCAATCATACCAATAAGTATT
GAAGGTATTAGCGATATAATAGGACAAAATGTTGATAAAGATGAAGTATTAAAAATATTAAAGAAATTAGGTTTTGAGCT
CATACTTTCAGGTGAAGGGCTTATAAATGTTAAAGCACCTTTACATCGTCCTGATATTAAAAATTTATCAGATATTTGCG
AGGAAGTTGTAAGAATTATAGGTATTGATAATATTGCTTCCAAGGGTTTAGAATTTATAGAAAAAAATCGTTTAAATTCT
GCATATAAAAATTATATTGAGTTTTTAAATTTAAGAAAAAGAGCAGTAGCTAGTGGTTATTTTGAAAGCTTACATTATGT
TTTGGATAATGGAGAAGAACTTAAAAGATTAGGTTTTGACTCTGTTAAATTAAAACTAATCAATCCAATTACAGCAGAAC
TTAATACTTTAAGAACAACCCTTTTAAATCATTTGTTAAATGCAGCAAGTTTAAATGCAAAAAATTCTAAAAAAATTATT
AAACTTTTTGAATTAGGGGCTGTTTTCAATGTTAATAATCAAGAATTAAATCGTATTGCATTTATCCATTCTGGCTTAAA
AGAAGAAGCTAAAATTTCCAACAAGGCCAAACCTGAATCGGTGCAATTTTATGATTTTCTTTTAGATATTAAAAATATTA
TAGGTGATTTTAAACTAAAATCATCAAAATATAATATTTTAAGCCCTTATGAACAAGCAGATATTTATCTATCGGATATT
AAAGTAGGTTTTATAGGCCGTTTGCATTTAAAAATAGAAAATGAGAGAGATTTACCAAAAACATATATTTGCGAACTTGA
TTTAGATTTAATCAGGCAAGATTTTAAAATAGCTAAGCCTTACTCCAAATTCCCAGCTATCACTAGAGATCTTAGCGTGT
TAATACCTAAAGGTTTTGAATACAATCAGATTAAAAATTGTATCGAAGAATTGAATTTAGAAATACTTGAAAATTTTCGT
TTAGTTGATATTTATAGTGATGAAAACCTTAAAGAATTTTATAGCATTACTATAAGTTTTTCTTTTAGGGATATAAATAA
AACTCTAGAAGATAATCAAGTGAATGAATGTATGGATAAAATTTTAAATACTCTTAAAAATTTGGGTCTTGATTTAAGAT
GA

Upstream 100 bases:

>100_bases
CTTTTGGATTGGGAGTAGAACGTTTTGCAATGCTTTTGCATCAAATTCCAGATTTAAGATCTTTATTTGAAGGTGATTTG
AGATTATTGGAGCAGTTTAG

Downstream 100 bases:

>100_bases
AAATTTACAAATTGCAAACCCCTGTAAATGCTATACTTGAAAATATAGCAGCAGATAAAAGCATATCTCATCGTTTTGCT
ATATTTTCGCTTTTAACACA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKAPDKVVVGYVKEKIKHENSDKLSICQVDIGSETLQIVCGA
ANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCSSLELGFEKINEGIMLLDESIGKLELGRPLNTYEIFNDELI
EVELTPNRGDCLSIYGIARDLAAALNLNLKEPKPFKESENVLGIGRILRLAAEKELNGLYNYRAIGLKEEIQTNLLLSLR
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFIINLSKQVHGETKVSYKDKLLSFSGIFQNNESRCKDDS
KIIIIEANYTDPLVIADAKIYHKDQDEKMLYRSFRGSEPKLNLGMDFLLGIFEHIPNLVIYSSSQQILTDKELPIIPISI
EGISDIIGQNVDKDEVLKILKKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIDNIASKGLEFIEKNRLNS
AYKNYIEFLNLRKRAVASGYFESLHYVLDNGEELKRLGFDSVKLKLINPITAELNTLRTTLLNHLLNAASLNAKNSKKII
KLFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPESVQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDI
KVGFIGRLHLKIENERDLPKTYICELDLDLIRQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEILENFR
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR

Sequences:

>Translated_773_residues
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKAPDKVVVGYVKEKIKHENSDKLSICQVDIGSETLQIVCGA
ANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCSSLELGFEKINEGIMLLDESIGKLELGRPLNTYEIFNDELI
EVELTPNRGDCLSIYGIARDLAAALNLNLKEPKPFKESENVLGIGRILRLAAEKELNGLYNYRAIGLKEEIQTNLLLSLR
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFIINLSKQVHGETKVSYKDKLLSFSGIFQNNESRCKDDS
KIIIIEANYTDPLVIADAKIYHKDQDEKMLYRSFRGSEPKLNLGMDFLLGIFEHIPNLVIYSSSQQILTDKELPIIPISI
EGISDIIGQNVDKDEVLKILKKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIDNIASKGLEFIEKNRLNS
AYKNYIEFLNLRKRAVASGYFESLHYVLDNGEELKRLGFDSVKLKLINPITAELNTLRTTLLNHLLNAASLNAKNSKKII
KLFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPESVQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDI
KVGFIGRLHLKIENERDLPKTYICELDLDLIRQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEILENFR
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR
>Mature_773_residues
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKAPDKVVVGYVKEKIKHENSDKLSICQVDIGSETLQIVCGA
ANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCSSLELGFEKINEGIMLLDESIGKLELGRPLNTYEIFNDELI
EVELTPNRGDCLSIYGIARDLAAALNLNLKEPKPFKESENVLGIGRILRLAAEKELNGLYNYRAIGLKEEIQTNLLLSLR
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFIINLSKQVHGETKVSYKDKLLSFSGIFQNNESRCKDDS
KIIIIEANYTDPLVIADAKIYHKDQDEKMLYRSFRGSEPKLNLGMDFLLGIFEHIPNLVIYSSSQQILTDKELPIIPISI
EGISDIIGQNVDKDEVLKILKKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIDNIASKGLEFIEKNRLNS
AYKNYIEFLNLRKRAVASGYFESLHYVLDNGEELKRLGFDSVKLKLINPITAELNTLRTTLLNHLLNAASLNAKNSKKII
KLFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPESVQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDI
KVGFIGRLHLKIENERDLPKTYICELDLDLIRQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEILENFR
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=805, Percent_Identity=27.3291925465839, Blast_Score=230, Evalue=3e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87284; Mature: 87284

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKAPDKVVVGYVKEKIKHENSD
CEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCC
KLSICQVDIGSETLQIVCGAANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCS
CEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
SLELGFEKINEGIMLLDESIGKLELGRPLNTYEIFNDELIEVELTPNRGDCLSIYGIARD
HHHHHHHHHCCCEEEEECCCCCEECCCCCCHHEECCCCEEEEEECCCCCCEEEHHHHHHH
LAAALNLNLKEPKPFKESENVLGIGRILRLAAEKELNGLYNYRAIGLKEEIQTNLLLSLR
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHH
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFIINLSKQVHGETKVSYKD
HHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHEEEHHHHHCCCCCCCHHH
KLLSFSGIFQNNESRCKDDSKIIIIEANYTDPLVIADAKIYHKDQDEKMLYRSFRGSEPK
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCEEECCCHHHHHHHHHCCCCCC
LNLGMDFLLGIFEHIPNLVIYSSSQQILTDKELPIIPISIEGISDIIGQNVDKDEVLKIL
CCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCEEEEEECCHHHHHCCCCCHHHHHHHH
KKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIDNIASKGLEFIEKNRLNS
HHCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AYKNYIEFLNLRKRAVASGYFESLHYVLDNGEELKRLGFDSVKLKLINPITAELNTLRTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LLNHLLNAASLNAKNSKKIIKLFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPES
HHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHHCCCCCCCC
VQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDIKVGFIGRLHLKIENERDLPK
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHCCEEEEEEEEEEEEEEEEEECCCCCCCC
TYICELDLDLIRQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEILENFR
EEEEECCHHHHHHHHHHCCCHHHCCHHHCCHHEEECCCCCHHHHHHHHHHHCHHHHCCEE
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR
EEEEECCCCCCEEEEEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKAPDKVVVGYVKEKIKHENSD
CEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCC
KLSICQVDIGSETLQIVCGAANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCS
CEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
SLELGFEKINEGIMLLDESIGKLELGRPLNTYEIFNDELIEVELTPNRGDCLSIYGIARD
HHHHHHHHHCCCEEEEECCCCCEECCCCCCHHEECCCCEEEEEECCCCCCEEEHHHHHHH
LAAALNLNLKEPKPFKESENVLGIGRILRLAAEKELNGLYNYRAIGLKEEIQTNLLLSLR
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHH
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFIINLSKQVHGETKVSYKD
HHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHEEEHHHHHCCCCCCCHHH
KLLSFSGIFQNNESRCKDDSKIIIIEANYTDPLVIADAKIYHKDQDEKMLYRSFRGSEPK
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCCEEECCCHHHHHHHHHCCCCCC
LNLGMDFLLGIFEHIPNLVIYSSSQQILTDKELPIIPISIEGISDIIGQNVDKDEVLKIL
CCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCEEEEEECCHHHHHCCCCCHHHHHHHH
KKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIDNIASKGLEFIEKNRLNS
HHCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AYKNYIEFLNLRKRAVASGYFESLHYVLDNGEELKRLGFDSVKLKLINPITAELNTLRTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LLNHLLNAASLNAKNSKKIIKLFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPES
HHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHHCCCCCCCC
VQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDIKVGFIGRLHLKIENERDLPK
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHCCEEEEEEEEEEEEEEEEEECCCCCCCC
TYICELDLDLIRQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEILENFR
EEEEECCHHHHHHHHHHCCCHHHCCHHHCCHHEEECCCCCHHHHHHHHHHHCHHHHCCEE
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR
EEEEECCCCCCEEEEEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]