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The map label for this gene is pyrG [H]

Identifier: 57236939

GI number: 57236939

Start: 32513

End: 34144

Strand: Direct

Name: pyrG [H]

Synonym: CJE0027

Alternate gene names: 57236939

Gene position: 32513-34144 (Clockwise)

Preceding gene: 57236936

Following gene: 57236940

Centisome position: 1.83

GC content: 34.56

Gene sequence:

>1632_bases
ATGAAACAAACAAAATATATTTTTGTAACAGGTGGAGTTTTAAGTTCTTTGGGTAAAGGGATAGCCGCTGCTTCTATCGC
AACACTTTTAAAAAATTCAGGGCTTAAAGTGAGTATTTTAAAAGCTGATCCTTATATCAATGTTGATCCAGGCACTATGA
GTCCTTTTGAGCATGGAGAGGTTTTTGTAACTGATGATGGAGCAGAAACGGATCTTGATTTGGGGCATTATGAGCGTTTT
TTGGATGAGAGTTTATCTCAAGATAATAACTTCACTACAGGACGGGTTTATCAAAGTGTGATTGAAAAAGAAAGGCGTGG
AGAATATCTTGGCAAAACCATTCAAGTTATCCCTCATATAGTAGGAGAAATCAAAGATCGCATTAAAAAAGCAGGAGAAG
GCAAAGATATACTTATCGTTGAAATAGGCGGAACTGTTGGGGATATAGAAGGCTTGCCTTTTTTAGAAGCTATTCGTGCT
TTGCGTTTGGAAGTGGGTAAAAATAATGCTATGAATATTCATCTTACTCTAGTACCTTTTATTAAGGCTGCAGGGGAATT
AAAAACAAAGCCAACTCAACATAGTGTAGGCGAGTTAAGGCGCATAGGCATTAGTCCTGATATGATTATTTGTCGTAGTG
AAAAAGCTCTTGATAGAGATTTAAAAGATAAAATTGCTATTTCTTGCGGGGTGGAAAAAAATTGCGTGATAGAAAGTGTT
GATGCGGCTAGTATTTATCAAATTCCACTTAATTTTTTAAAGCAAGATATTTTAAGCCCTATTGCTGAAATTTTGGATTT
GAAAAATTTAAAACCTAATATGGAAAATTGGGACTCTTTGGTAAAAAGAGTTATAGCTCCAAGCAATGAAGTTAAAATCG
CTTTTGTAGGCAAATATGTGGATTTAAAAGAAAGTTATAAAAGCCTTACTGAAGCTATTATACACGCAGGTGCAGCCCTA
GATACTAAAGTGGAGTTAAAATGGGTAGATAGTGAAAAACTTGAAAATATGGAAAGTTCTGAAGTATTTAAAGATGTGAG
TGGAATTTTGGTTGCTGGGGGATTTGGATATCGTGGTGTAGAAGGTAAGATAAAAGCCATTCAATACGCAAGAGAAAATA
AAATTCCTTTTTTAGGAATTTGTTTAGGTATGCAGCTTGCTTTGGTGGAATTTGCTAGAAATGTATTAAAACTTAAAGAT
GCAAATTCAAGTGAATTTAATGAAAAATGCCAAAATCCTGTGGTTTATTTGATTGATGAATTTATGGATACAAATGGAGA
AAAACAAATTCGTACTGCAAAAACTCCTTTAGGTGGAACTATGCGTTTAGGGGCTTATAAATGTGATATCAAGGAAAAAT
CCCTTTTGGCTAAGGTTTATAATGAAGTAAAGAGTGTAAAAGAACGCCATCGTCATCGCTATGAAGCAAATCCAAAATAT
AGAGCAGATTTTGAAAAACACGGTTTGATTGTAAGTGGTGAAAGCAAAGGATTGATTGAAGCAGTTGAGCTTAACTGTCA
TCCTTTTTTCTTGGCTGTGCAGTTTCATCCAGAATTTACATCAAGACTTGAGCATGTTAATCCTGTGATTTGCAGTTTTA
TCAAGGCAGCTATAAACTATGAAGATAATTAA

Upstream 100 bases:

>100_bases
AAAATATTTTTTGTAATTCTTGCATTTTTTACTTAAAATTCTAATGAAATAAAAAGTTTTATCAAGCTATAATTAGATAA
AAAAATAAGGCTTATTATCA

Downstream 100 bases:

>100_bases
TAAAAATGAAATTAAAAAAATTTTGGCTTCACGCTTTGAAAAAGATTTGCATACCAAACTTTGTGATCTGCCTTTGCCAT
GCTGTTTAAAGGATGCATAT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MKQTKYIFVTGGVLSSLGKGIAAASIATLLKNSGLKVSILKADPYINVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERF
LDESLSQDNNFTTGRVYQSVIEKERRGEYLGKTIQVIPHIVGEIKDRIKKAGEGKDILIVEIGGTVGDIEGLPFLEAIRA
LRLEVGKNNAMNIHLTLVPFIKAAGELKTKPTQHSVGELRRIGISPDMIICRSEKALDRDLKDKIAISCGVEKNCVIESV
DAASIYQIPLNFLKQDILSPIAEILDLKNLKPNMENWDSLVKRVIAPSNEVKIAFVGKYVDLKESYKSLTEAIIHAGAAL
DTKVELKWVDSEKLENMESSEVFKDVSGILVAGGFGYRGVEGKIKAIQYARENKIPFLGICLGMQLALVEFARNVLKLKD
ANSSEFNEKCQNPVVYLIDEFMDTNGEKQIRTAKTPLGGTMRLGAYKCDIKEKSLLAKVYNEVKSVKERHRHRYEANPKY
RADFEKHGLIVSGESKGLIEAVELNCHPFFLAVQFHPEFTSRLEHVNPVICSFIKAAINYEDN

Sequences:

>Translated_543_residues
MKQTKYIFVTGGVLSSLGKGIAAASIATLLKNSGLKVSILKADPYINVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERF
LDESLSQDNNFTTGRVYQSVIEKERRGEYLGKTIQVIPHIVGEIKDRIKKAGEGKDILIVEIGGTVGDIEGLPFLEAIRA
LRLEVGKNNAMNIHLTLVPFIKAAGELKTKPTQHSVGELRRIGISPDMIICRSEKALDRDLKDKIAISCGVEKNCVIESV
DAASIYQIPLNFLKQDILSPIAEILDLKNLKPNMENWDSLVKRVIAPSNEVKIAFVGKYVDLKESYKSLTEAIIHAGAAL
DTKVELKWVDSEKLENMESSEVFKDVSGILVAGGFGYRGVEGKIKAIQYARENKIPFLGICLGMQLALVEFARNVLKLKD
ANSSEFNEKCQNPVVYLIDEFMDTNGEKQIRTAKTPLGGTMRLGAYKCDIKEKSLLAKVYNEVKSVKERHRHRYEANPKY
RADFEKHGLIVSGESKGLIEAVELNCHPFFLAVQFHPEFTSRLEHVNPVICSFIKAAINYEDN
>Mature_543_residues
MKQTKYIFVTGGVLSSLGKGIAAASIATLLKNSGLKVSILKADPYINVDPGTMSPFEHGEVFVTDDGAETDLDLGHYERF
LDESLSQDNNFTTGRVYQSVIEKERRGEYLGKTIQVIPHIVGEIKDRIKKAGEGKDILIVEIGGTVGDIEGLPFLEAIRA
LRLEVGKNNAMNIHLTLVPFIKAAGELKTKPTQHSVGELRRIGISPDMIICRSEKALDRDLKDKIAISCGVEKNCVIESV
DAASIYQIPLNFLKQDILSPIAEILDLKNLKPNMENWDSLVKRVIAPSNEVKIAFVGKYVDLKESYKSLTEAIIHAGAAL
DTKVELKWVDSEKLENMESSEVFKDVSGILVAGGFGYRGVEGKIKAIQYARENKIPFLGICLGMQLALVEFARNVLKLKD
ANSSEFNEKCQNPVVYLIDEFMDTNGEKQIRTAKTPLGGTMRLGAYKCDIKEKSLLAKVYNEVKSVKERHRHRYEANPKY
RADFEKHGLIVSGESKGLIEAVELNCHPFFLAVQFHPEFTSRLEHVNPVICSFIKAAINYEDN

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=558, Percent_Identity=45.5197132616487, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI28559085, Length=560, Percent_Identity=45.5357142857143, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI28559083, Length=560, Percent_Identity=45.5357142857143, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI221316689, Length=560, Percent_Identity=45.5357142857143, Blast_Score=478, Evalue=1e-135,
Organism=Escherichia coli, GI1789142, Length=540, Percent_Identity=52.7777777777778, Blast_Score=569, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI25148299, Length=611, Percent_Identity=40.0981996726678, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322563, Length=571, Percent_Identity=43.7828371278459, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6319432, Length=554, Percent_Identity=43.3212996389892, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=47.0483005366726, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI21357815, Length=504, Percent_Identity=45.2380952380952, Blast_Score=410, Evalue=1e-114,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60347; Mature: 60347

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTKYIFVTGGVLSSLGKGIAAASIATLLKNSGLKVSILKADPYINVDPGTMSPFEHGE
CCCCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCE
VFVTDDGAETDLDLGHYERFLDESLSQDNNFTTGRVYQSVIEKERRGEYLGKTIQVIPHI
EEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGEIKDRIKKAGEGKDILIVEIGGTVGDIEGLPFLEAIRALRLEVGKNNAMNIHLTLVPF
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEHH
IKAAGELKTKPTQHSVGELRRIGISPDMIICRSEKALDRDLKDKIAISCGVEKNCVIESV
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCHHHCCCHHHHEEEECCCCCCCEEECC
DAASIYQIPLNFLKQDILSPIAEILDLKNLKPNMENWDSLVKRVIAPSNEVKIAFVGKYV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECHH
DLKESYKSLTEAIIHAGAALDTKVELKWVDSEKLENMESSEVFKDVSGILVAGGFGYRGV
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCHHHHHHHHHCCEEEECCCCCCCC
EGKIKAIQYARENKIPFLGICLGMQLALVEFARNVLKLKDANSSEFNEKCQNPVVYLIDE
CCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEHH
FMDTNGEKQIRTAKTPLGGTMRLGAYKCDIKEKSLLAKVYNEVKSVKERHRHRYEANPKY
HHCCCCCCEEEECCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RADFEKHGLIVSGESKGLIEAVELNCHPFFLAVQFHPEFTSRLEHVNPVICSFIKAAINY
CCCHHHCCEEEECCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHCCHHHHHHHHHHHCC
EDN
CCC
>Mature Secondary Structure
MKQTKYIFVTGGVLSSLGKGIAAASIATLLKNSGLKVSILKADPYINVDPGTMSPFEHGE
CCCCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCE
VFVTDDGAETDLDLGHYERFLDESLSQDNNFTTGRVYQSVIEKERRGEYLGKTIQVIPHI
EEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGEIKDRIKKAGEGKDILIVEIGGTVGDIEGLPFLEAIRALRLEVGKNNAMNIHLTLVPF
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEHH
IKAAGELKTKPTQHSVGELRRIGISPDMIICRSEKALDRDLKDKIAISCGVEKNCVIESV
HHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCHHHCCCHHHHEEEECCCCCCCEEECC
DAASIYQIPLNFLKQDILSPIAEILDLKNLKPNMENWDSLVKRVIAPSNEVKIAFVGKYV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECHH
DLKESYKSLTEAIIHAGAALDTKVELKWVDSEKLENMESSEVFKDVSGILVAGGFGYRGV
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCHHHHHHHHHCCEEEECCCCCCCC
EGKIKAIQYARENKIPFLGICLGMQLALVEFARNVLKLKDANSSEFNEKCQNPVVYLIDE
CCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEHH
FMDTNGEKQIRTAKTPLGGTMRLGAYKCDIKEKSLLAKVYNEVKSVKERHRHRYEANPKY
HHCCCCCCEEEECCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RADFEKHGLIVSGESKGLIEAVELNCHPFFLAVQFHPEFTSRLEHVNPVICSFIKAAINY
CCCHHHCCEEEECCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHCCHHHHHHHHHHHCC
EDN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA