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The map label for this gene is punA [H]

Identifier: 51891974

GI number: 51891974

Start: 942692

End: 943513

Strand: Reverse

Name: punA [H]

Synonym: STH836

Alternate gene names: 51891974

Gene position: 943513-942692 (Counterclockwise)

Preceding gene: 51891980

Following gene: 51891972

Centisome position: 26.46

GC content: 70.07

Gene sequence:

>822_bases
ATGTCCGACCTGTTGCAGCGCATCGAGGAAGCCCGCGCGGCCGTGGCCGCCAGGGGCGGCGTCACGCCGCAAGTCGGGCT
TATCCTGGGTTCCGGCCTCGGCGACCTGGCCGACCAGGTGGAGGACGCCGTGAAGGTCCCGTACAACGAGATTCCCCACT
TCCCCGTTTCCACCGCGCCCGGCCACGCAGGCCGGCTGGTGATCGGCCGCCTGGAAGGAAAGCCGGTGGTCGCCATGCAG
GGGCGCGTCCACTTCTACGAGGGCTATACCATGGAGCAGGTCACCTTCCCGGTGCGCGTCATGCGTGCCCTGGGCGTCGA
GACCCTGATCGTGACCTGCGCCGCGGGCGGGCTCAACCCGTCGTTCTCCGCCGGCGACCTCATGCTGATCACCGACCACA
TCAACTTCATGGGGCAGGACCCGCTGCGGGGCCCCAACGACGAGCGGCTCGGCCCGCGCTTCCCGGCCACCGTCGGCGCC
TACACGCCGGAGCTGCGTGAGCTGGCCGTTGCGGTGGCGCAGGAGATGGGCGTCTCTCTGCGGCAGGGAACGTACACGGC
CATCTCAGGGCCTTGCTACAACACCAGCGCGGAGCTCCGGATGCTCCAGCGGTTGGGCGGCGACGCCGTCGGCATGTCCA
CGGTGCCGGAGGTCGTGACGGCCCGGCACATGGGGCTGCGGGTGCTCGGCATCGCCTGCATCACGGACATGGCGCTGCCC
GACGCGCCGGTGCACCTGACCCACGAGCAGGTGCTGGAAGTCGCCAACCGCACGAAGCCCCTGTTCCAGAACCTGGTGCG
CGGCGTGGTGCGGCGGATCTGA

Upstream 100 bases:

>100_bases
TTAGGTTTGTCTACAGAAGTTGCGTGTCCCGTGCGCTGGCCGTCCGCGGGCGCATCAGCCGCCTGCCGGCCAGACACCCT
ACCAAGCGAGGTGTTGACCT

Downstream 100 bases:

>100_bases
CGGCCCGGCGGGCGGAGGGCCCGCGCGCAGCCCGGCCACGGGCGAGGCGCCGATCACAGGAGGGCGGCCTGCAGGACACA
CCGGCCGTGTGCCTGCAGGC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSDLLQRIEEARAAVAARGGVTPQVGLILGSGLGDLADQVEDAVKVPYNEIPHFPVSTAPGHAGRLVIGRLEGKPVVAMQ
GRVHFYEGYTMEQVTFPVRVMRALGVETLIVTCAAGGLNPSFSAGDLMLITDHINFMGQDPLRGPNDERLGPRFPATVGA
YTPELRELAVAVAQEMGVSLRQGTYTAISGPCYNTSAELRMLQRLGGDAVGMSTVPEVVTARHMGLRVLGIACITDMALP
DAPVHLTHEQVLEVANRTKPLFQNLVRGVVRRI

Sequences:

>Translated_273_residues
MSDLLQRIEEARAAVAARGGVTPQVGLILGSGLGDLADQVEDAVKVPYNEIPHFPVSTAPGHAGRLVIGRLEGKPVVAMQ
GRVHFYEGYTMEQVTFPVRVMRALGVETLIVTCAAGGLNPSFSAGDLMLITDHINFMGQDPLRGPNDERLGPRFPATVGA
YTPELRELAVAVAQEMGVSLRQGTYTAISGPCYNTSAELRMLQRLGGDAVGMSTVPEVVTARHMGLRVLGIACITDMALP
DAPVHLTHEQVLEVANRTKPLFQNLVRGVVRRI
>Mature_272_residues
SDLLQRIEEARAAVAARGGVTPQVGLILGSGLGDLADQVEDAVKVPYNEIPHFPVSTAPGHAGRLVIGRLEGKPVVAMQG
RVHFYEGYTMEQVTFPVRVMRALGVETLIVTCAAGGLNPSFSAGDLMLITDHINFMGQDPLRGPNDERLGPRFPATVGAY
TPELRELAVAVAQEMGVSLRQGTYTAISGPCYNTSAELRMLQRLGGDAVGMSTVPEVVTARHMGLRVLGIACITDMALPD
APVHLTHEQVLEVANRTKPLFQNLVRGVVRRI

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=251, Percent_Identity=54.1832669322709, Blast_Score=267, Evalue=7e-72,
Organism=Homo sapiens, GI47132622, Length=252, Percent_Identity=26.984126984127, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1788746, Length=252, Percent_Identity=49.6031746031746, Blast_Score=245, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17541190, Length=256, Percent_Identity=42.1875, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI71980569, Length=250, Percent_Identity=26.8, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323238, Length=308, Percent_Identity=43.1818181818182, Blast_Score=220, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=46.9090909090909, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24656093, Length=275, Percent_Identity=46.9090909090909, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24656090, Length=275, Percent_Identity=46.9090909090909, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=46.9090909090909, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=38.0753138075314, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI20130079, Length=228, Percent_Identity=28.5087719298246, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29255; Mature: 29124

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLQRIEEARAAVAARGGVTPQVGLILGSGLGDLADQVEDAVKVPYNEIPHFPVSTAP
CHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCC
GHAGRLVIGRLEGKPVVAMQGRVHFYEGYTMEQVTFPVRVMRALGVETLIVTCAAGGLNP
CCCCEEEEEECCCCEEEEEECEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
SFSAGDLMLITDHINFMGQDPLRGPNDERLGPRFPATVGAYTPELRELAVAVAQEMGVSL
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCHH
RQGTYTAISGPCYNTSAELRMLQRLGGDAVGMSTVPEVVTARHMGLRVLGIACITDMALP
HCCCEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAPVHLTHEQVLEVANRTKPLFQNLVRGVVRRI
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDLLQRIEEARAAVAARGGVTPQVGLILGSGLGDLADQVEDAVKVPYNEIPHFPVSTAP
HHHHHHHHHHHHHHHHCCCCCCCHHHEEECCHHHHHHHHHHHHHCCHHHCCCCCCCCCC
GHAGRLVIGRLEGKPVVAMQGRVHFYEGYTMEQVTFPVRVMRALGVETLIVTCAAGGLNP
CCCCEEEEEECCCCEEEEEECEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCC
SFSAGDLMLITDHINFMGQDPLRGPNDERLGPRFPATVGAYTPELRELAVAVAQEMGVSL
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHCCHH
RQGTYTAISGPCYNTSAELRMLQRLGGDAVGMSTVPEVVTARHMGLRVLGIACITDMALP
HCCCEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAPVHLTHEQVLEVANRTKPLFQNLVRGVVRRI
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]