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The map label for this gene is rsmF [H]

Identifier: 51891972

GI number: 51891972

Start: 940647

End: 942125

Strand: Reverse

Name: rsmF [H]

Synonym: STH834

Alternate gene names: 51891972

Gene position: 942125-940647 (Counterclockwise)

Preceding gene: 51891974

Following gene: 51891969

Centisome position: 26.42

GC content: 73.9

Gene sequence:

>1479_bases
ATGTTTGAATCGCCTGACAAGGGGGTGGTTGGCCTGGAGGATCGCGCACTCCCCCAGCCGTTCGTCGCCCGCATGTCCGC
GTTGCTGCAGGACGAGTTCCCCGCCTTCCTGGCGACGTACAGCTCGCCCCCGGAACGGGGGATCCGGGCGAACCGGCTGA
AGATCGCACGGGAGGAGCTGGCAGAGCGCATGGGACTTACGGCCGAACCGGTGCCCTGGAATCCGGACGGCCTGTACTGC
CCGCCTTCGCTGCGCCCCGGCTGGCACCCGTACCATGCGGCCGGGCTTTACTACGGGCAGGAACCCAGCGCAATGGTGGT
CGCGCCGCTGGTCGGGGCCCGACCCGGCGAGCGGGTGCTGGACCTGGCCGCGGCCCCTGGGGGCAAGACGACCCAGCTCG
CGGCGCAGATGGAAAACCGCGGCCTCCTCGTGGCCAACGAGGTCGACCCCGGCCGGGTGCGCGCCCTGGTCGAGAACCTG
GAGCGGCTGGGCATCACGTGCGCCGCGGTGGTGAGCGAGCGGCCCGAGCGGCTGGCGGAAAGGCTGCCGGGTTTCTTCGA
CCGGGTCCTGGTGGACGCCCCCTGCTCCGGGGAGGGCATGTTCCGCAAGACGCCCGAGGTCCGCGACCGCTGGCGACCCG
ACATGCCGGAGGCCTGCGCCCGCGTGCAGGGGGAGATCCTGAACAGCGCCGTGGAGCTCCTCCGCCCCGGCGGGCGGCTG
GTCTACTCCACCTGCACCTTCGCCCCCGAGGAGAACGAGGAGGTCCTGCTTCAGTTGCTCACGAGGCGGGCCGACCTGCG
CATGCTCCCCCTCCCCCCGGTTCCCGGGATCGCGCCCGGGCGTCCCGAATGGACCCGCGATCCGGCGCGGGCGGCCACCG
TCGGCGTCGGGCTGGCCGGGCGGCTCTGGCCCCACCGGGTGCGGGGCGAGGGCCACTTCGTCGCGGTGCTGGAGCGGGTC
GCGGACGGCGCGGGCCGGCAGCGCCCGCCGCTCCGCAGGGATTGGCAGCGGCCGTCCAAGGAGGCGCTGGCGGACTTCCG
CACGTTCTGCCGCGAGACGCTGACACCGGCCGGACAGGACGCGCTGCCGCCGGAGGCGGAACTGCGGCAGCACGGAGAGT
GGCTCTTTGCGCCTCCGGCAGACAGCCGGGCGCTGGTGGGGATGCACGTGGTGCGCGCCGGCCTGCAGCTCGGCCGGGCG
CTCAGGGGCCGGTTCGAACCCTCACATCCGCTGGCCCTGGCGCTGCGGCCCGGGCACGTGCAGCGGGTGCGCGACCTGGC
CGCCGGCTCCCCCGAACTCCTGGCCTACCTGCGGGGCGAGACCCTGCCCGCCGGCGGCGAGCGCGGCTGGACGCTGGTCA
CGGTCGACGGCTTCCCCCTGGGCTGGGCCAAGGCGGCCAACGGGATCCTGAAGAACCACTACCCCAAGGTCCTGCGCTGG
GACGCCGGGGCGCCGGAGCCCATCGACCCCGAAAGCTGA

Upstream 100 bases:

>100_bases
AGCGCCCGCCTGGCCCGCTCCGCGACGCTGCTCAGGGCCTCTTCAGCCTGGGCCATACCGATCACGCCTCCCTCGCATTC
CCTATGCTGTTATTATATGG

Downstream 100 bases:

>100_bases
AGACCGCCGGGCTGCGCATACAGCCCGGCGGTCCGTGATGTTCGGGGAAGGCGGTGCGGTCGGGCCTGCGGTCCTCCGGG
GCCCGGCTCAGTCGTGGGTC

Product: putative rRNA methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MFESPDKGVVGLEDRALPQPFVARMSALLQDEFPAFLATYSSPPERGIRANRLKIAREELAERMGLTAEPVPWNPDGLYC
PPSLRPGWHPYHAAGLYYGQEPSAMVVAPLVGARPGERVLDLAAAPGGKTTQLAAQMENRGLLVANEVDPGRVRALVENL
ERLGITCAAVVSERPERLAERLPGFFDRVLVDAPCSGEGMFRKTPEVRDRWRPDMPEACARVQGEILNSAVELLRPGGRL
VYSTCTFAPEENEEVLLQLLTRRADLRMLPLPPVPGIAPGRPEWTRDPARAATVGVGLAGRLWPHRVRGEGHFVAVLERV
ADGAGRQRPPLRRDWQRPSKEALADFRTFCRETLTPAGQDALPPEAELRQHGEWLFAPPADSRALVGMHVVRAGLQLGRA
LRGRFEPSHPLALALRPGHVQRVRDLAAGSPELLAYLRGETLPAGGERGWTLVTVDGFPLGWAKAANGILKNHYPKVLRW
DAGAPEPIDPES

Sequences:

>Translated_492_residues
MFESPDKGVVGLEDRALPQPFVARMSALLQDEFPAFLATYSSPPERGIRANRLKIAREELAERMGLTAEPVPWNPDGLYC
PPSLRPGWHPYHAAGLYYGQEPSAMVVAPLVGARPGERVLDLAAAPGGKTTQLAAQMENRGLLVANEVDPGRVRALVENL
ERLGITCAAVVSERPERLAERLPGFFDRVLVDAPCSGEGMFRKTPEVRDRWRPDMPEACARVQGEILNSAVELLRPGGRL
VYSTCTFAPEENEEVLLQLLTRRADLRMLPLPPVPGIAPGRPEWTRDPARAATVGVGLAGRLWPHRVRGEGHFVAVLERV
ADGAGRQRPPLRRDWQRPSKEALADFRTFCRETLTPAGQDALPPEAELRQHGEWLFAPPADSRALVGMHVVRAGLQLGRA
LRGRFEPSHPLALALRPGHVQRVRDLAAGSPELLAYLRGETLPAGGERGWTLVTVDGFPLGWAKAANGILKNHYPKVLRW
DAGAPEPIDPES
>Mature_492_residues
MFESPDKGVVGLEDRALPQPFVARMSALLQDEFPAFLATYSSPPERGIRANRLKIAREELAERMGLTAEPVPWNPDGLYC
PPSLRPGWHPYHAAGLYYGQEPSAMVVAPLVGARPGERVLDLAAAPGGKTTQLAAQMENRGLLVANEVDPGRVRALVENL
ERLGITCAAVVSERPERLAERLPGFFDRVLVDAPCSGEGMFRKTPEVRDRWRPDMPEACARVQGEILNSAVELLRPGGRL
VYSTCTFAPEENEEVLLQLLTRRADLRMLPLPPVPGIAPGRPEWTRDPARAATVGVGLAGRLWPHRVRGEGHFVAVLERV
ADGAGRQRPPLRRDWQRPSKEALADFRTFCRETLTPAGQDALPPEAELRQHGEWLFAPPADSRALVGMHVVRAGLQLGRA
LRGRFEPSHPLALALRPGHVQRVRDLAAGSPELLAYLRGETLPAGGERGWTLVTVDGFPLGWAKAANGILKNHYPKVLRW
DAGAPEPIDPES

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=308, Percent_Identity=34.0909090909091, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI76150623, Length=308, Percent_Identity=34.0909090909091, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI39995082, Length=325, Percent_Identity=29.2307692307692, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI301336155, Length=280, Percent_Identity=31.0714285714286, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI32698918, Length=208, Percent_Identity=35.0961538461538, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI11545785, Length=227, Percent_Identity=34.8017621145374, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI270288816, Length=292, Percent_Identity=30.8219178082192, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI8922322, Length=256, Percent_Identity=31.640625, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI270288818, Length=256, Percent_Identity=31.640625, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI23199998, Length=256, Percent_Identity=31.640625, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI40316918, Length=184, Percent_Identity=29.8913043478261, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87081985, Length=482, Percent_Identity=31.5352697095436, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI2367212, Length=265, Percent_Identity=31.3207547169811, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17536757, Length=320, Percent_Identity=31.875, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI25143471, Length=270, Percent_Identity=31.4814814814815, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI115534021, Length=225, Percent_Identity=31.1111111111111, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71998419, Length=225, Percent_Identity=31.1111111111111, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=321, Percent_Identity=33.6448598130841, Blast_Score=156, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6319447, Length=274, Percent_Identity=31.7518248175182, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI22024126, Length=284, Percent_Identity=33.4507042253521, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21355201, Length=315, Percent_Identity=31.4285714285714, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356513, Length=197, Percent_Identity=33.502538071066, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24668781, Length=226, Percent_Identity=31.4159292035398, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920494, Length=169, Percent_Identity=31.3609467455621, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356579, Length=182, Percent_Identity=27.4725274725275, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53944; Mature: 53944

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFESPDKGVVGLEDRALPQPFVARMSALLQDEFPAFLATYSSPPERGIRANRLKIAREEL
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHH
AERMGLTAEPVPWNPDGLYCPPSLRPGWHPYHAAGLYYGQEPSAMVVAPLVGARPGERVL
HHHHCCCCCCCCCCCCCEECCCCCCCCCCCHHHCCEEECCCCCCEEEEHHHCCCCCHHHH
DLAAAPGGKTTQLAAQMENRGLLVANEVDPGRVRALVENLERLGITCAAVVSERPERLAE
HHHCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLPGFFDRVLVDAPCSGEGMFRKTPEVRDRWRPDMPEACARVQGEILNSAVELLRPGGRL
HCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VYSTCTFAPEENEEVLLQLLTRRADLRMLPLPPVPGIAPGRPEWTRDPARAATVGVGLAG
EEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
RLWPHRVRGEGHFVAVLERVADGAGRQRPPLRRDWQRPSKEALADFRTFCRETLTPAGQD
CCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
ALPPEAELRQHGEWLFAPPADSRALVGMHVVRAGLQLGRALRGRFEPSHPLALALRPGHV
CCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHH
QRVRDLAAGSPELLAYLRGETLPAGGERGWTLVTVDGFPLGWAKAANGILKNHYPKVLRW
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHCCCCEEEE
DAGAPEPIDPES
CCCCCCCCCCCC
>Mature Secondary Structure
MFESPDKGVVGLEDRALPQPFVARMSALLQDEFPAFLATYSSPPERGIRANRLKIAREEL
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHH
AERMGLTAEPVPWNPDGLYCPPSLRPGWHPYHAAGLYYGQEPSAMVVAPLVGARPGERVL
HHHHCCCCCCCCCCCCCEECCCCCCCCCCCHHHCCEEECCCCCCEEEEHHHCCCCCHHHH
DLAAAPGGKTTQLAAQMENRGLLVANEVDPGRVRALVENLERLGITCAAVVSERPERLAE
HHHCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RLPGFFDRVLVDAPCSGEGMFRKTPEVRDRWRPDMPEACARVQGEILNSAVELLRPGGRL
HCCHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VYSTCTFAPEENEEVLLQLLTRRADLRMLPLPPVPGIAPGRPEWTRDPARAATVGVGLAG
EEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
RLWPHRVRGEGHFVAVLERVADGAGRQRPPLRRDWQRPSKEALADFRTFCRETLTPAGQD
CCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
ALPPEAELRQHGEWLFAPPADSRALVGMHVVRAGLQLGRALRGRFEPSHPLALALRPGHV
CCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHH
QRVRDLAAGSPELLAYLRGETLPAGGERGWTLVTVDGFPLGWAKAANGILKNHYPKVLRW
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCHHHHCCCCEEEE
DAGAPEPIDPES
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA