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The map label for this gene is dfrA

Identifier: 41408966

GI number: 41408966

Start: 3195689

End: 3196234

Strand: Reverse

Name: dfrA

Synonym: MAP2868c

Alternate gene names: 41408966

Gene position: 3196234-3195689 (Counterclockwise)

Preceding gene: 41408967

Following gene: 41408965

Centisome position: 66.18

GC content: 73.44

Gene sequence:

>546_bases
ATGACCCGTGCCGAGGTGGGCCTGGTGTGGGCCCAGTCGACGTCTGGCGTCATCGGCCGCGGCGGTGACATCCCGTGGAG
CGTGCCCGAAGACCTCACCCGGTTCAAAGAGGTGACCATGGGGCACACGGTGATCATGGGCCGGCGGACCTGGGAGTCGT
TGCCGGCCAAGGTGCGGCCGCTGCCCGGCCGGCGCAACGTGGTGGTGTCGCGCCGGCCCGACTTCGTCGCCGAGGGGGCG
CGGGTGGCCGGGTCGCTGGAGGCGGCCCTCGCGCACGCCGGGAGCGACCCGGCGCCGTGGGTGATCGGCGGCGCGCAGAT
CTATCTGCTGGCGCTGCCGCATGCCACCCGCTGCGAGGTCACCGAGATCGAGATCGACCTGCGCCGCGACGACGACGACG
CGCTGGCGCCGGCGCTGGACGACAGCTGGGTGGGCGAGACGGGCGAGTGGCTGGCCAGCCGCTCCGGGCTGCGGTACCGG
TTCCACAGCTACCGTCGGGACCCGCGCTCTTCCGTTCGCGGCTGTTCGCCCTCACGCCCGAGCTGA

Upstream 100 bases:

>100_bases
TCATTCTGGCGCATAGGGATTCGATCTTCGACTACACCTACGACGACGTCGTGGTGCACAACTACGATCCGCACCCGGCC
ATCAAGGCCCCCGTCGCGGT

Downstream 100 bases:

>100_bases
CATACTCGGACGCGGGGGTCGGTCACACACCGTCTACCAGCCGCTGTTCGGGAAAAGGGGGAATTGGCAGTGATCACCGA
ACCCGTCAAGACGTTTTCGC

Product: DfrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MTRAEVGLVWAQSTSGVIGRGGDIPWSVPEDLTRFKEVTMGHTVIMGRRTWESLPAKVRPLPGRRNVVVSRRPDFVAEGA
RVAGSLEAALAHAGSDPAPWVIGGAQIYLLALPHATRCEVTEIEIDLRRDDDDALAPALDDSWVGETGEWLASRSGLRYR
FHSYRRDPRSSVRGCSPSRPS

Sequences:

>Translated_181_residues
MTRAEVGLVWAQSTSGVIGRGGDIPWSVPEDLTRFKEVTMGHTVIMGRRTWESLPAKVRPLPGRRNVVVSRRPDFVAEGA
RVAGSLEAALAHAGSDPAPWVIGGAQIYLLALPHATRCEVTEIEIDLRRDDDDALAPALDDSWVGETGEWLASRSGLRYR
FHSYRRDPRSSVRGCSPSRPS
>Mature_180_residues
TRAEVGLVWAQSTSGVIGRGGDIPWSVPEDLTRFKEVTMGHTVIMGRRTWESLPAKVRPLPGRRNVVVSRRPDFVAEGAR
VAGSLEAALAHAGSDPAPWVIGGAQIYLLALPHATRCEVTEIEIDLRRDDDDALAPALDDSWVGETGEWLASRSGLRYRF
HSYRRDPRSSVRGCSPSRPS

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=185, Percent_Identity=28.6486486486486, Blast_Score=71, Evalue=6e-13,
Organism=Escherichia coli, GI1786233, Length=127, Percent_Identity=38.5826771653543, Blast_Score=84, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=31.6939890710383, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324810, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI116008026, Length=151, Percent_Identity=29.8013245033113, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24647458, Length=151, Percent_Identity=29.8013245033113, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19857; Mature: 19725

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAEVGLVWAQSTSGVIGRGGDIPWSVPEDLTRFKEVTMGHTVIMGRRTWESLPAKVRP
CCCCCEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHCCEEEEEECCCHHHCCCEECC
LPGRRNVVVSRRPDFVAEGARVAGSLEAALAHAGSDPAPWVIGGAQIYLLALPHATRCEV
CCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCEE
TEIEIDLRRDDDDALAPALDDSWVGETGEWLASRSGLRYRFHSYRRDPRSSVRGCSPSRP
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEHHHHCCCHHHHHCCCCCCCC
S
C
>Mature Secondary Structure 
TRAEVGLVWAQSTSGVIGRGGDIPWSVPEDLTRFKEVTMGHTVIMGRRTWESLPAKVRP
CCCCEEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHCCEEEEEECCCHHHCCCEECC
LPGRRNVVVSRRPDFVAEGARVAGSLEAALAHAGSDPAPWVIGGAQIYLLALPHATRCEV
CCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCEE
TEIEIDLRRDDDDALAPALDDSWVGETGEWLASRSGLRYRFHSYRRDPRSSVRGCSPSRP
EEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEHHHHCCCHHHHHCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]