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The map label for this gene is gyrB [H]

Identifier: 39995114

GI number: 39995114

Start: 3901

End: 6288

Strand: Direct

Name: gyrB [H]

Synonym: GSU0003

Alternate gene names: 39995114

Gene position: 3901-6288 (Clockwise)

Preceding gene: 39995113

Following gene: 39995115

Centisome position: 0.1

GC content: 61.31

Gene sequence:

>2388_bases
ATGACCGACGAACAGAACTACGATTACGGTGCAGACAAGATCAAGGTCCTGGAGGGGCTCGAGGCGGTACGCAAGCGCCC
CGCCATGTACATCGGCTCCACCGCGGCCCAGGGGCTCCACCACCTGGTCTACGAGATCGTGGACAACTCCATTGACGAGG
CCCTGGCCGGCTACTGCAACGAGGTGAACGTCACCATCCATCTGGATGGTTCCCTGACGGTGGTGGACAATGGCCGCGGC
ATTCCTACCGACATCCACCCCACGGAGGGGAAGCCGGCGGCGGAGGTGGCCCTCACGGTCCTCCATGCCGGCGGCAAGTT
CGACAACACCTCCTATAAGGTCTCCGGCGGGCTCCACGGGGTCGGGGTCTCGGTCGTGAACGCCCTGTCCAGGAAGCTGG
AGCTGGAGATTCGCCGGGACGGGAAGGTGTACCGCCAGTCCTACGCCTGCGGCGACCCCCAGACTCCCCTCGAAGTGATG
GGGGAAACGAAAAAGCGTGGGACCAAAATAACCTTCCTCCCCGACGATACCATCTTCGAGACCACGGAGTTTTCCTTCGA
CGTTCTTTCCCAGCGGCTCCGGGAGCTGGCGTTCCTCAACGCCGGGGTCCGGATCACCATCACCGACGAACGGGAGGAGG
GAAAGCACCACGACTTTCACTACGAAGGAGGCATCGTCTCCTTTGTGGAGTACCTGAACCGCAACCGGACCGCCCTCCAC
CCGAAGCCGATCTACTTCAAGGGTGAAAAGGCAGGAGTCGAGATGGAGGTGGCCCTCCAGTACAACGATTCCTATGACGA
AAAGGTTTTCTCTTTTGCCAACAACATCAACACCCACGAGGGTGGAACCCACTTGGTGGGGTTCCGGGCGGCCCTCACCC
GGACCATGAACACCTATGCCGCCGCCAACGAACTCCTCAAAAAGGAGAAGGTTGCCATCTCCGGCGAGGATCTGCGAGAG
GGTCTCACGGCGGTCATCTCGGTCAAGATTCCCCAACCACAGTTCGAGGGGCAGACCAAGACCAAGCTGGGGAACTCGGA
GGTTAAGGGGTACGTGGAATCCCTCATGAACGAGAAACTGGCGGTCTTCCTGGAAGAGAACCCGAAGATCGCCAAAGACA
TCATCGGCAAATCCATCGAGGCGGCCCGTGCCCGGGAAGCGGCCCGCAAGGCGCGGGAATTGACCCGGCGCAAGGGCGCC
CTGGACATCTCCAACCTTCCGGGCAAACTGGCCGACTGCCAGGAGCGGGATCCGGCCCTGTGCGAACTCTACCTGGTGGA
GGGCGACTCCGCCGGCGGTTCCGCCAAACAGGGGCGCGACCGGAAATTCCAGGCCATTCTGCCGCTGAAAGGAAAGATCC
TCAACGTGGAGAAGGCCCGGTTCGACAAGATGCTTTCCTCCCAGGAGATCGGGACCCTTATCACGGCGCTCGGCACCGGT
ATCGGGAAAGACGACTTCGACATCGCCAAGCTCCGCTACCACCGGATCATCGTCATGACCGACGCCGACGTGGACGGCTC
CCACATCCTGACGCTGCTGCTCACCTTCTTCTTCCGGCAGATGCCGGAGGTGGTGGAGCGGGGGCACCTCTACATCGCCC
AGCCCCCCCTCTATAAGGTGAAGCGAGGGCGCAAGGAGCTCTATCTCCGCAATGAGGCGGCCATGCAGGCCTACCTCCTT
GAGGAGGGGACCGAGGACATGCTCCTTTTTCTGGAGAGCGGCGAGAAGACCTACAGCGGCAAGCAGATCATCCCGATCCT
CAAGCAGTTGGTGGAGTATCGGACCATCCTGGATAAGGTAGTGCGCAAGGGGATCAACGAGGAGCTCATACGGGTATTCC
TGCGGCTCGGCGTCAAGGGGGGGATTGAGGACATGGAGCAGTTGGTGCCGTTCCTGACGAACATCTCCCGGGTCTATGAC
GGCGGCGATTTCAGCCCCTTGGACGACGGGCGGGTCATCGTGCGGCTCGGCAACCTCCGGATCGCCCTGGATCAGCATAC
GCTCGACCTCATCGGTTCCTACGAGTACGGGCTCCTGGTGGAGAGCTACCGAAAGGTCAGGGATATCTTCGGCGACGGCC
GGGCCGTGGTCTCCAGCGAGGGTAAGGAACTCTTCGCGACCACCATCGGGCTCGACCTCCTCTCCTTCTTCATGGACAGC
GCCAAGAAGGGGCTCTCCATCCAGCGCTACAAGGGTCTCGGCGAGATGAACCCGGAGCAGCTCTGGGAAACCACCATGGA
GCCCGTCAACCGGACCCTGCTCCAGGTGAAGATTGAGGACGCCATCGAGGCAGACACCATATTCACCATCCTCATGGGGG
ACCAGGTGGAGCCCCGCCGGGACTTCATCGAGCAGAACGCCCTGAACGTGTCGAATTTGGACATTTAG

Upstream 100 bases:

>100_bases
ACCACCACCGATGTATCAACCCTCCGTCTGGAGGGGATCGACACCCACGCGTCGTTTCGCGTTTCACGTGGAACGGTGAC
GCCTGTATAGAGGGAGAGGC

Downstream 100 bases:

>100_bases
ACGATTATGTGAGGGGTGTGATTCATCGCGCCCGGGTGCAATGAATCACACCCCTACGGGTGCCATGCCGTTATCGGGCG
AGGCATCGTCTCGACCCTAC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MTDEQNYDYGADKIKVLEGLEAVRKRPAMYIGSTAAQGLHHLVYEIVDNSIDEALAGYCNEVNVTIHLDGSLTVVDNGRG
IPTDIHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHGVGVSVVNALSRKLELEIRRDGKVYRQSYACGDPQTPLEVM
GETKKRGTKITFLPDDTIFETTEFSFDVLSQRLRELAFLNAGVRITITDEREEGKHHDFHYEGGIVSFVEYLNRNRTALH
PKPIYFKGEKAGVEMEVALQYNDSYDEKVFSFANNINTHEGGTHLVGFRAALTRTMNTYAAANELLKKEKVAISGEDLRE
GLTAVISVKIPQPQFEGQTKTKLGNSEVKGYVESLMNEKLAVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKGA
LDISNLPGKLADCQERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTG
IGKDDFDIAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKVKRGRKELYLRNEAAMQAYLL
EEGTEDMLLFLESGEKTYSGKQIIPILKQLVEYRTILDKVVRKGINEELIRVFLRLGVKGGIEDMEQLVPFLTNISRVYD
GGDFSPLDDGRVIVRLGNLRIALDQHTLDLIGSYEYGLLVESYRKVRDIFGDGRAVVSSEGKELFATTIGLDLLSFFMDS
AKKGLSIQRYKGLGEMNPEQLWETTMEPVNRTLLQVKIEDAIEADTIFTILMGDQVEPRRDFIEQNALNVSNLDI

Sequences:

>Translated_795_residues
MTDEQNYDYGADKIKVLEGLEAVRKRPAMYIGSTAAQGLHHLVYEIVDNSIDEALAGYCNEVNVTIHLDGSLTVVDNGRG
IPTDIHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHGVGVSVVNALSRKLELEIRRDGKVYRQSYACGDPQTPLEVM
GETKKRGTKITFLPDDTIFETTEFSFDVLSQRLRELAFLNAGVRITITDEREEGKHHDFHYEGGIVSFVEYLNRNRTALH
PKPIYFKGEKAGVEMEVALQYNDSYDEKVFSFANNINTHEGGTHLVGFRAALTRTMNTYAAANELLKKEKVAISGEDLRE
GLTAVISVKIPQPQFEGQTKTKLGNSEVKGYVESLMNEKLAVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKGA
LDISNLPGKLADCQERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTG
IGKDDFDIAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKVKRGRKELYLRNEAAMQAYLL
EEGTEDMLLFLESGEKTYSGKQIIPILKQLVEYRTILDKVVRKGINEELIRVFLRLGVKGGIEDMEQLVPFLTNISRVYD
GGDFSPLDDGRVIVRLGNLRIALDQHTLDLIGSYEYGLLVESYRKVRDIFGDGRAVVSSEGKELFATTIGLDLLSFFMDS
AKKGLSIQRYKGLGEMNPEQLWETTMEPVNRTLLQVKIEDAIEADTIFTILMGDQVEPRRDFIEQNALNVSNLDI
>Mature_794_residues
TDEQNYDYGADKIKVLEGLEAVRKRPAMYIGSTAAQGLHHLVYEIVDNSIDEALAGYCNEVNVTIHLDGSLTVVDNGRGI
PTDIHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHGVGVSVVNALSRKLELEIRRDGKVYRQSYACGDPQTPLEVMG
ETKKRGTKITFLPDDTIFETTEFSFDVLSQRLRELAFLNAGVRITITDEREEGKHHDFHYEGGIVSFVEYLNRNRTALHP
KPIYFKGEKAGVEMEVALQYNDSYDEKVFSFANNINTHEGGTHLVGFRAALTRTMNTYAAANELLKKEKVAISGEDLREG
LTAVISVKIPQPQFEGQTKTKLGNSEVKGYVESLMNEKLAVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKGAL
DISNLPGKLADCQERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTGI
GKDDFDIAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKVKRGRKELYLRNEAAMQAYLLE
EGTEDMLLFLESGEKTYSGKQIIPILKQLVEYRTILDKVVRKGINEELIRVFLRLGVKGGIEDMEQLVPFLTNISRVYDG
GDFSPLDDGRVIVRLGNLRIALDQHTLDLIGSYEYGLLVESYRKVRDIFGDGRAVVSSEGKELFATTIGLDLLSFFMDSA
KKGLSIQRYKGLGEMNPEQLWETTMEPVNRTLLQVKIEDAIEADTIFTILMGDQVEPRRDFIEQNALNVSNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=580, Percent_Identity=25.5172413793103, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI19913408, Length=588, Percent_Identity=26.0204081632653, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI48994957, Length=813, Percent_Identity=55.5965559655597, Blast_Score=835, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=555, Percent_Identity=41.6216216216216, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI212645845, Length=511, Percent_Identity=27.2015655577299, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17535065, Length=578, Percent_Identity=25.2595155709343, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=146, Percent_Identity=32.1917808219178, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=585, Percent_Identity=27.8632478632479, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17136538, Length=575, Percent_Identity=25.9130434782609, Blast_Score=137, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 88788; Mature: 88656

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEQNYDYGADKIKVLEGLEAVRKRPAMYIGSTAAQGLHHLVYEIVDNSIDEALAGYCN
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCC
EVNVTIHLDGSLTVVDNGRGIPTDIHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHG
CEEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCEEECCCCCH
VGVSVVNALSRKLELEIRRDGKVYRQSYACGDPQTPLEVMGETKKRGTKITFLPDDTIFE
HHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCCCHHHHCCHHHCCCEEEECCCCCEEC
TTEFSFDVLSQRLRELAFLNAGVRITITDEREEGKHHDFHYEGGIVSFVEYLNRNRTALH
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCHHHHHHHHCCCCCCCC
PKPIYFKGEKAGVEMEVALQYNDSYDEKVFSFANNINTHEGGTHLVGFRAALTRTMNTYA
CCCEEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
AANELLKKEKVAISGEDLREGLTAVISVKIPQPQFEGQTKTKLGNSEVKGYVESLMNEKL
HHHHHHHHHHHEECCHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHCCCE
AVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKGALDISNLPGKLADCQERDPAL
EEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
CELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTG
EEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGKDDFDIAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKV
CCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
KRGRKELYLRNEAAMQAYLLEEGTEDMLLFLESGEKTYSGKQIIPILKQLVEYRTILDKV
HCCCHHEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
VRKGINEELIRVFLRLGVKGGIEDMEQLVPFLTNISRVYDGGDFSPLDDGRVIVRLGNLR
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEE
IALDQHTLDLIGSYEYGLLVESYRKVRDIFGDGRAVVSSEGKELFATTIGLDLLSFFMDS
EEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
AKKGLSIQRYKGLGEMNPEQLWETTMEPVNRTLLQVKIEDAIEADTIFTILMGDQVEPRR
HHCCCEEHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEHHHHCCCCEEEEEECCCCCHHH
DFIEQNALNVSNLDI
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDEQNYDYGADKIKVLEGLEAVRKRPAMYIGSTAAQGLHHLVYEIVDNSIDEALAGYCN
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCC
EVNVTIHLDGSLTVVDNGRGIPTDIHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHG
CEEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCEEECCCCCH
VGVSVVNALSRKLELEIRRDGKVYRQSYACGDPQTPLEVMGETKKRGTKITFLPDDTIFE
HHHHHHHHHHHHEEEEEECCCHHHHHHCCCCCCCCCHHHHCCHHHCCCEEEECCCCCEEC
TTEFSFDVLSQRLRELAFLNAGVRITITDEREEGKHHDFHYEGGIVSFVEYLNRNRTALH
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCHHHHHHHHCCCCCCCC
PKPIYFKGEKAGVEMEVALQYNDSYDEKVFSFANNINTHEGGTHLVGFRAALTRTMNTYA
CCCEEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
AANELLKKEKVAISGEDLREGLTAVISVKIPQPQFEGQTKTKLGNSEVKGYVESLMNEKL
HHHHHHHHHHHEECCHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHCCCE
AVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKGALDISNLPGKLADCQERDPAL
EEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
CELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTG
EEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGKDDFDIAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKV
CCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
KRGRKELYLRNEAAMQAYLLEEGTEDMLLFLESGEKTYSGKQIIPILKQLVEYRTILDKV
HCCCHHEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
VRKGINEELIRVFLRLGVKGGIEDMEQLVPFLTNISRVYDGGDFSPLDDGRVIVRLGNLR
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEE
IALDQHTLDLIGSYEYGLLVESYRKVRDIFGDGRAVVSSEGKELFATTIGLDLLSFFMDS
EEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
AKKGLSIQRYKGLGEMNPEQLWETTMEPVNRTLLQVKIEDAIEADTIFTILMGDQVEPRR
HHCCCEEHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEHHHHCCCCEEEEEECCCCCHHH
DFIEQNALNVSNLDI
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]