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The map label for this gene is tgt [H]

Identifier: 38232874

GI number: 38232874

Start: 214616

End: 215908

Strand: Direct

Name: tgt [H]

Synonym: DIP0251

Alternate gene names: 38232874

Gene position: 214616-215908 (Clockwise)

Preceding gene: 38232873

Following gene: 38232875

Centisome position: 8.62

GC content: 60.09

Gene sequence:

>1293_bases
ATGACTGACACGACTTTTGAGCTTCGTACCGAGCTGGACGACGCGCCAGGCCGCCACGGCCGAACTGGTGTAATCCATAC
CCCTCATGGTGACATCAACACGCCGGCGTTCATCCCTGTGGCCACCAAGGCGACGGTGAAAACCCTCACCCCACAGCAAA
TTCGTGACACTGGCGCCCAGGCGATCCTCTCCAACGCCTACCACCTCTACTTGCAGCCGGGCCCCGATATTGTCGATGAA
GCCGGCGGTGTATCCAAGTTCGAGAACTGGCACGGCCCCACCTACACCGACTCCGGCGGATTCCAGGTGATGAGCCTTGG
TGTTGGCTTTAAAAAGGTGCTCGCTATGGATACCGCGGGGCTTGTAGAAGGCGACATCCGCGCGCCTAAAAAAGATCGCT
ACGCGCAGGTTGATGAAGACGGCGTGGACTTCCGCAGCGTTATCGACGGCTCCCGCCACCGCTTCACCCCAGAAGTCAGC
ATGCAAATCCAGCACCGGCTGGGCGCCGACATCATCTTCGCTTTCGACGAGCTGACCACCCTCGTGGACACCCGCCAGTA
CCAAGAAAGCTCCGTCGAACGCACCCACCGCTGGGCACGCCGCTGCCTCATCGAACACGACCGCCTCACCCGCGAGCGCG
AAGGAAAACCACTGCAATCCCTGTGGGGCGTTGTCCAAGGCGCCCAGTACGAGGATCTGCGCAGACAAGCAGTCCGTGGC
CTGCTTGACCTAGACAAAGAGGCAGAAGATAACGGACGACGCGGCTTCGGTGGCTTCGGCATCGGTGGCGCATTAGAAAA
GGAAAACCTAGGAACCATCGTAGGCTGGGTCTGCGACGAAATCCCGCAGCACAAACCACGCCACCTACTGGGAATCAGCG
AACCAGACGACCTCTTTACCGCCATCGAAGCAGGTGCCGACACCTTCGACTGTGTTGCGCCGACGCGCCTCGGCCGTCGC
GGCGGCGTGTACACCCTCGACGGGCGCGTGAACCTCACCGCAGCCCGCTTCAAACGCGACTTCCGTCAAGTAGACGAGGA
GTTCGGCGGCCCTATCGCCGAGTACTCCCGCGCCTACATTCACCACCTGTTCAAGGCCAAGGAATTCCTCGGTGGAACAT
TGTGCACAATGCATAACGTGGCCTTTATGATCCAGCTGGTAGACAACATTCGCAGCGCTATCAACAACGGCGACTTTGAA
GCCTACCGCGATGAATTCCTCGGCCGCTACTACGCAAGCAAAGGAGGCCCAGCAGCAGCTGGGCGCTCCGGAGGGATGAT
CTACGGGGTTTAG

Upstream 100 bases:

>100_bases
ATCACAAGAGCTCTGTGGCTATGAAACGTAGCGCAAGCACCAACTACTGTTTGTTCCCCCAAAGTGCCTGTCCACGTGCA
ACAAGCTAGAATTTCTCTCT

Downstream 100 bases:

>100_bases
CGCATAAGCATGGGTACTTTTCAGGGAGCAGGGCGCTATGCGCCAAGTCCAAGCGGGGATCTTCACTTTGGCAACGTGCG
CACCGCTGTGTTGGCGTGGC

Product: putative tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTDTTFELRTELDDAPGRHGRTGVIHTPHGDINTPAFIPVATKATVKTLTPQQIRDTGAQAILSNAYHLYLQPGPDIVDE
AGGVSKFENWHGPTYTDSGGFQVMSLGVGFKKVLAMDTAGLVEGDIRAPKKDRYAQVDEDGVDFRSVIDGSRHRFTPEVS
MQIQHRLGADIIFAFDELTTLVDTRQYQESSVERTHRWARRCLIEHDRLTREREGKPLQSLWGVVQGAQYEDLRRQAVRG
LLDLDKEAEDNGRRGFGGFGIGGALEKENLGTIVGWVCDEIPQHKPRHLLGISEPDDLFTAIEAGADTFDCVAPTRLGRR
GGVYTLDGRVNLTAARFKRDFRQVDEEFGGPIAEYSRAYIHHLFKAKEFLGGTLCTMHNVAFMIQLVDNIRSAINNGDFE
AYRDEFLGRYYASKGGPAAAGRSGGMIYGV

Sequences:

>Translated_430_residues
MTDTTFELRTELDDAPGRHGRTGVIHTPHGDINTPAFIPVATKATVKTLTPQQIRDTGAQAILSNAYHLYLQPGPDIVDE
AGGVSKFENWHGPTYTDSGGFQVMSLGVGFKKVLAMDTAGLVEGDIRAPKKDRYAQVDEDGVDFRSVIDGSRHRFTPEVS
MQIQHRLGADIIFAFDELTTLVDTRQYQESSVERTHRWARRCLIEHDRLTREREGKPLQSLWGVVQGAQYEDLRRQAVRG
LLDLDKEAEDNGRRGFGGFGIGGALEKENLGTIVGWVCDEIPQHKPRHLLGISEPDDLFTAIEAGADTFDCVAPTRLGRR
GGVYTLDGRVNLTAARFKRDFRQVDEEFGGPIAEYSRAYIHHLFKAKEFLGGTLCTMHNVAFMIQLVDNIRSAINNGDFE
AYRDEFLGRYYASKGGPAAAGRSGGMIYGV
>Mature_429_residues
TDTTFELRTELDDAPGRHGRTGVIHTPHGDINTPAFIPVATKATVKTLTPQQIRDTGAQAILSNAYHLYLQPGPDIVDEA
GGVSKFENWHGPTYTDSGGFQVMSLGVGFKKVLAMDTAGLVEGDIRAPKKDRYAQVDEDGVDFRSVIDGSRHRFTPEVSM
QIQHRLGADIIFAFDELTTLVDTRQYQESSVERTHRWARRCLIEHDRLTREREGKPLQSLWGVVQGAQYEDLRRQAVRGL
LDLDKEAEDNGRRGFGGFGIGGALEKENLGTIVGWVCDEIPQHKPRHLLGISEPDDLFTAIEAGADTFDCVAPTRLGRRG
GVYTLDGRVNLTAARFKRDFRQVDEEFGGPIAEYSRAYIHHLFKAKEFLGGTLCTMHNVAFMIQLVDNIRSAINNGDFEA
YRDEFLGRYYASKGGPAAAGRSGGMIYGV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=396, Percent_Identity=32.8282828282828, Blast_Score=227, Evalue=1e-59,
Organism=Escherichia coli, GI1786607, Length=406, Percent_Identity=38.1773399014778, Blast_Score=266, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17544680, Length=394, Percent_Identity=32.2335025380711, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI19920510, Length=400, Percent_Identity=32.75, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 47676; Mature: 47545

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTTFELRTELDDAPGRHGRTGVIHTPHGDINTPAFIPVATKATVKTLTPQQIRDTGAQ
CCCCCCHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCHHHHHCCHHHHHHHHHH
AILSNAYHLYLQPGPDIVDEAGGVSKFENWHGPTYTDSGGFQVMSLGVGFKKVLAMDTAG
HHHCCCEEEEECCCCCHHHCCCCCHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCC
LVEGDIRAPKKDRYAQVDEDGVDFRSVIDGSRHRFTPEVSMQIQHRLGADIIFAFDELTT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHH
LVDTRQYQESSVERTHRWARRCLIEHDRLTREREGKPLQSLWGVVQGAQYEDLRRQAVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LLDLDKEAEDNGRRGFGGFGIGGALEKENLGTIVGWVCDEIPQHKPRHLLGISEPDDLFT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHH
AIEAGADTFDCVAPTRLGRRGGVYTLDGRVNLTAARFKRDFRQVDEEFGGPIAEYSRAYI
HHHCCCCCCHHCCCHHCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HHLFKAKEFLGGTLCTMHNVAFMIQLVDNIRSAINNGDFEAYRDEFLGRYYASKGGPAAA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
GRSGGMIYGV
CCCCCEEEEC
>Mature Secondary Structure 
TDTTFELRTELDDAPGRHGRTGVIHTPHGDINTPAFIPVATKATVKTLTPQQIRDTGAQ
CCCCCHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCHHHHHCCHHHHHHHHHH
AILSNAYHLYLQPGPDIVDEAGGVSKFENWHGPTYTDSGGFQVMSLGVGFKKVLAMDTAG
HHHCCCEEEEECCCCCHHHCCCCCHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCC
LVEGDIRAPKKDRYAQVDEDGVDFRSVIDGSRHRFTPEVSMQIQHRLGADIIFAFDELTT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHH
LVDTRQYQESSVERTHRWARRCLIEHDRLTREREGKPLQSLWGVVQGAQYEDLRRQAVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LLDLDKEAEDNGRRGFGGFGIGGALEKENLGTIVGWVCDEIPQHKPRHLLGISEPDDLFT
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHH
AIEAGADTFDCVAPTRLGRRGGVYTLDGRVNLTAARFKRDFRQVDEEFGGPIAEYSRAYI
HHHCCCCCCHHCCCHHCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HHLFKAKEFLGGTLCTMHNVAFMIQLVDNIRSAINNGDFEAYRDEFLGRYYASKGGPAAA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
GRSGGMIYGV
CCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA