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The map label for this gene is gluQ

Identifier: 38232875

GI number: 38232875

Start: 215918

End: 216811

Strand: Direct

Name: gluQ

Synonym: DIP0252

Alternate gene names: 38232875

Gene position: 215918-216811 (Clockwise)

Preceding gene: 38232874

Following gene: 38232876

Centisome position: 8.68

GC content: 62.08

Gene sequence:

>894_bases
ATGGGTACTTTTCAGGGAGCAGGGCGCTATGCGCCAAGTCCAAGCGGGGATCTTCACTTTGGCAACGTGCGCACCGCTGT
GTTGGCGTGGCTGTTTGCCCGCCACACTGGTCGTCGCTTCCTTATTCGCGTAGAAGATGTGGACACGCAGCGTTCGTCCC
TGGAATCTGCCGCCCGTCAGCTGGAGGACCTGCATGCGCTGGGCATGGATTGGGACGCCGCCCCTACCTATCAGCACAAC
AACTTTGACCGCTATGAGCAGGCGTTACGCAACCTTCCGCATTATGAGTGCTACTGCTCGCGTAAGGACATCCAAGAGGC
CTCCCGCGCGCCGCACACCATCCCGGGCCAGTACCCCGGCACGTGCCGAAATTTAACTGAGGTGCAGCGGGAGGAGCGTC
GTCAAGCATTAGCCAAGCAGGGGAGGGTGCCGGCGCTGCGTTTGCGTGCCGACGTTCCCACGTGGCACGTCCGCGACTAC
TATGCGGGGGACGTGCTGGGCGACGTAGACGACATGATCCTGCGCCGCGGCGGCCAGCAACCTGACTGGGCGTACAACCT
TGCCGTTGTGGTCGACGATGCCGCCGACGGTATCGACCAAGTGGTTCGCGGCAACGACCTGCTCACCTCTGCGCCGCGCC
AGGCCTACCTTGCGCACCTACTCGGCGTGCCGGCCCCAACCTATGTGCATGTGCCGCTGGTGCTCAACGCGGCGGGGCAG
CGCCTAGCCAAGCGCGATGGTGCCGTGACCATGCGCGAGATGCCCGACATTCTGCCGCGCGTTGCCGAATCTCTAGGGCT
AAGCGCGCGTACTCTGCCTGGCATGCTCGAAGAATTCGATCCCGATAGGTTCTCGCAACAACCATGGATTTTTGCAATCC
ATAAGGAGGTATAA

Upstream 100 bases:

>100_bases
CTACCGCGATGAATTCCTCGGCCGCTACTACGCAAGCAAAGGAGGCCCAGCAGCAGCTGGGCGCTCCGGAGGGATGATCT
ACGGGGTTTAGCGCATAAGC

Downstream 100 bases:

>100_bases
GTCTCATATAAGTGTGGAGTATGCATGCATACACATACATTAGAGGGGTCTTTTATTTTGTAACCAAGGAGAAAATCCGA
TGGAAGATTGGGTTCCCACC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGTFQGAGRYAPSPSGDLHFGNVRTAVLAWLFARHTGRRFLIRVEDVDTQRSSLESAARQLEDLHALGMDWDAAPTYQHN
NFDRYEQALRNLPHYECYCSRKDIQEASRAPHTIPGQYPGTCRNLTEVQREERRQALAKQGRVPALRLRADVPTWHVRDY
YAGDVLGDVDDMILRRGGQQPDWAYNLAVVVDDAADGIDQVVRGNDLLTSAPRQAYLAHLLGVPAPTYVHVPLVLNAAGQ
RLAKRDGAVTMREMPDILPRVAESLGLSARTLPGMLEEFDPDRFSQQPWIFAIHKEV

Sequences:

>Translated_297_residues
MGTFQGAGRYAPSPSGDLHFGNVRTAVLAWLFARHTGRRFLIRVEDVDTQRSSLESAARQLEDLHALGMDWDAAPTYQHN
NFDRYEQALRNLPHYECYCSRKDIQEASRAPHTIPGQYPGTCRNLTEVQREERRQALAKQGRVPALRLRADVPTWHVRDY
YAGDVLGDVDDMILRRGGQQPDWAYNLAVVVDDAADGIDQVVRGNDLLTSAPRQAYLAHLLGVPAPTYVHVPLVLNAAGQ
RLAKRDGAVTMREMPDILPRVAESLGLSARTLPGMLEEFDPDRFSQQPWIFAIHKEV
>Mature_296_residues
GTFQGAGRYAPSPSGDLHFGNVRTAVLAWLFARHTGRRFLIRVEDVDTQRSSLESAARQLEDLHALGMDWDAAPTYQHNN
FDRYEQALRNLPHYECYCSRKDIQEASRAPHTIPGQYPGTCRNLTEVQREERRQALAKQGRVPALRLRADVPTWHVRDYY
AGDVLGDVDDMILRRGGQQPDWAYNLAVVVDDAADGIDQVVRGNDLLTSAPRQAYLAHLLGVPAPTYVHVPLVLNAAGQR
LAKRDGAVTMREMPDILPRVAESLGLSARTLPGMLEEFDPDRFSQQPWIFAIHKEV

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=335, Percent_Identity=30.4477611940298, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1788743, Length=262, Percent_Identity=35.4961832061069, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=36.5758754863813, Blast_Score=134, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=297, Percent_Identity=28.956228956229, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324540, Length=266, Percent_Identity=31.203007518797, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24665321, Length=264, Percent_Identity=31.4393939393939, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLUQ_CORDI (Q6NJY9)

Other databases:

- EMBL:   BX248354
- RefSeq:   NP_938642.1
- ProteinModelPortal:   Q6NJY9
- SMR:   Q6NJY9
- GeneID:   2650581
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0252
- NMPDR:   fig|257309.1.peg.233
- HOGENOM:   HBG628189
- OMA:   DAPSSYH
- ProtClustDB:   PRK05710
- BioCyc:   CDIP257309:DIP0252-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: 6.1.1.17

Molecular weight: Translated: 33391; Mature: 33260

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 45-45 BINDING 185-185 BINDING 203-203 BINDING 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTFQGAGRYAPSPSGDLHFGNVRTAVLAWLFARHTGRRFLIRVEDVDTQRSSLESAARQ
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
LEDLHALGMDWDAAPTYQHNNFDRYEQALRNLPHYECYCSRKDIQEASRAPHTIPGQYPG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCCCCCCC
TCRNLTEVQREERRQALAKQGRVPALRLRADVPTWHVRDYYAGDVLGDVDDMILRRGGQQ
CHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCHHHCCCHHCCHHHHHHHCCCCC
PDWAYNLAVVVDDAADGIDQVVRGNDLLTSAPRQAYLAHLLGVPAPTYVHVPLVLNAAGQ
CCCEEEEEEEEECHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
RLAKRDGAVTMREMPDILPRVAESLGLSARTLPGMLEEFDPDRFSQQPWIFAIHKEV
HHHHHCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
GTFQGAGRYAPSPSGDLHFGNVRTAVLAWLFARHTGRRFLIRVEDVDTQRSSLESAARQ
CCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHH
LEDLHALGMDWDAAPTYQHNNFDRYEQALRNLPHYECYCSRKDIQEASRAPHTIPGQYPG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCCCCCCCC
TCRNLTEVQREERRQALAKQGRVPALRLRADVPTWHVRDYYAGDVLGDVDDMILRRGGQQ
CHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCHHHCCCHHCCHHHHHHHCCCCC
PDWAYNLAVVVDDAADGIDQVVRGNDLLTSAPRQAYLAHLLGVPAPTYVHVPLVLNAAGQ
CCCEEEEEEEEECHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
RLAKRDGAVTMREMPDILPRVAESLGLSARTLPGMLEEFDPDRFSQQPWIFAIHKEV
HHHHHCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910