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The map label for this gene is mrpA [H]

Identifier: 239618416

GI number: 239618416

Start: 2185443

End: 2187278

Strand: Reverse

Name: mrpA [H]

Synonym: Kole_2056

Alternate gene names: 239618416

Gene position: 2187278-2185443 (Counterclockwise)

Preceding gene: 239618417

Following gene: 239618415

Centisome position: 95.01

GC content: 40.96

Gene sequence:

>1836_bases
ATGAGTTTAACCGTTCTCCTGACAATTAGTTTGATATCAGCACCGATTCTATACCTTCTGGCAAAAGTAAACAAAAACTT
CGCATATACGGTTTATGCGTTGATACAGCTTTTCGCATTTTCTGCGATATTTTTTAACTGGATCTATATTCCCGAGCATT
CGGGATTGAGAATTTTTTCCAACCTCGAACTGACGTTCAATGCCTCTATCTTGAATAGATTTTTTGCCTCAGTTACTATG
CTAATAATCTCAATGACTGCGGTATTCATGCTTTCGATCAAGAAAAAGAAAGAAATGAAGTTAATGCTTTTGAGTCTTGT
AAGTACCGGTGTAATCGGAAGTATGCTATCGAATGATTTCCTGACATTACTTATATTTTGGGAGTTAACAACATGGTCCT
CATTGATCATGATTGTCCTCGATAAAAAGATAGCCTGGAAGGAAGCTCTCAAGTATGTAGCAGTCGGTTCAATAGGAACC
TATTCAATGATGTACGCAGTATTTCTTCTTTACAACCGCTTTGGAACGCTCATCTATACAGATGTTGCAAATGAACTTAT
GAAAACTTCTGGAGGATTTCAACTGTTAATCTTCATTTTATTAGCGGTAATGGCTTTTGCAAAAGCTGGAAGTTTTCCAT
TGCACATCTGGTTGAGGGGAGCACATTCCTCCGCTCCGGACGAATTCAGCTCTATACTATCAGGAGCTTTAACGAAAATG
GGAGCTTTCTTGATGTTCATCGTCGTTGCGGTACTTCCATCGACTAAATTTTTTGCATGGATGCCTGAGGTTCAGGGAAT
TCCGCTGCCAAACTATATACTCGCCCTTCTAAGTGGCATCTCGATAATCGTCGGTACGGTGATGGCAATCCGAATGGAAG
ACGCCAAGCAATTGATCGCTTTTTCTACAGTAAGTAATTCCGGATATATTTTGATGGCACTAGCCATAGGCGGGCCGTAT
GCCACTGCTGGGGGATTGATGCATGTTCTAAATCACGCGCTTGCCTCTTCAGCTATGTTTCTCGCCATAGCGGCTGTTGC
TCATCGAACAGGAACAACTAAGATGCACGAAATGGGTGGTTTGATTTTCAGAATGCCTTTCACTTTCACGGCTTATCTTG
TAGCAATAATCTCCATTGCCGGGATACCACCAATGAGTGGCTTCGCTTCGAAATGGTTGATATACCAACAATTGGTACGT
AATGGTTTACCATTCCTTGCTTTTATGGCGTTCTTTGGAAGCATCGGTTCTTTCATGTACGTGTTCAAACCTCTTGCCGG
CATATTCCTTGGACAGTTGAAACCACAACATAAGAACATAAAAGAGGCACCGATACCGATGCTGGTATCTATGATGGTTC
TCACTCTTCTAACGATATTCTGGGGAATACTTCCCAGCAACGCTATATCATTCACGAATAAAATAAGTGAACAGGTTGGA
ATCGCCACGATAAAAACAACCTTTTCTGAAATCTTTGCTTCAACCGGAAAATGGGATTCAATCATAGTTGTAACGGTGTT
CACCATAGGTTTTGTTATAGCATTTGTTATAAGTGCTCTCGCAAAGAAGCCAAAAAAGGTAGATTTAATGGATACATACA
CAGGTGGGGAGTTCATTTACACTGCGGAACTCTATCACTTTTCTTACAAAATGTACCGTTCATTCGACAGAATGTTCGAA
CGCTGGCCTTTGATGGAAAAATGGCTTGAAAATACGTCTTATAAACTGAGAGAACTTGGAGCCTTTTTCCGTAGCCTCTT
CTATCCTTCAAGCCCACAAGGCTATGTAACTTTGAGCATTCTTGCAATACTAACAGCCTATTGGTGGTTGAGGTGA

Upstream 100 bases:

>100_bases
AATAGCTTCACAAGGAGCTTTTGAAGTATTTGAAAAAGCTGGAACAGCCATACTCGATAGCTTATCCTATGCTTCTACGG
TTCTTCTGGGAGGGATTTGA

Downstream 100 bases:

>100_bases
TAGCATGACAGTGATAAAAAGTATTGGATTACTTTTATTAGCTTTCGTTTATACGATAACGCTGGAAGGTATTGCCAGAA
AAATAGTTGCAAGGATAGAA

Product: NADH dehydrogenase subunit M

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSLTVLLTISLISAPILYLLAKVNKNFAYTVYALIQLFAFSAIFFNWIYIPEHSGLRIFSNLELTFNASILNRFFASVTM
LIISMTAVFMLSIKKKKEMKLMLLSLVSTGVIGSMLSNDFLTLLIFWELTTWSSLIMIVLDKKIAWKEALKYVAVGSIGT
YSMMYAVFLLYNRFGTLIYTDVANELMKTSGGFQLLIFILLAVMAFAKAGSFPLHIWLRGAHSSAPDEFSSILSGALTKM
GAFLMFIVVAVLPSTKFFAWMPEVQGIPLPNYILALLSGISIIVGTVMAIRMEDAKQLIAFSTVSNSGYILMALAIGGPY
ATAGGLMHVLNHALASSAMFLAIAAVAHRTGTTKMHEMGGLIFRMPFTFTAYLVAIISIAGIPPMSGFASKWLIYQQLVR
NGLPFLAFMAFFGSIGSFMYVFKPLAGIFLGQLKPQHKNIKEAPIPMLVSMMVLTLLTIFWGILPSNAISFTNKISEQVG
IATIKTTFSEIFASTGKWDSIIVVTVFTIGFVIAFVISALAKKPKKVDLMDTYTGGEFIYTAELYHFSYKMYRSFDRMFE
RWPLMEKWLENTSYKLRELGAFFRSLFYPSSPQGYVTLSILAILTAYWWLR

Sequences:

>Translated_611_residues
MSLTVLLTISLISAPILYLLAKVNKNFAYTVYALIQLFAFSAIFFNWIYIPEHSGLRIFSNLELTFNASILNRFFASVTM
LIISMTAVFMLSIKKKKEMKLMLLSLVSTGVIGSMLSNDFLTLLIFWELTTWSSLIMIVLDKKIAWKEALKYVAVGSIGT
YSMMYAVFLLYNRFGTLIYTDVANELMKTSGGFQLLIFILLAVMAFAKAGSFPLHIWLRGAHSSAPDEFSSILSGALTKM
GAFLMFIVVAVLPSTKFFAWMPEVQGIPLPNYILALLSGISIIVGTVMAIRMEDAKQLIAFSTVSNSGYILMALAIGGPY
ATAGGLMHVLNHALASSAMFLAIAAVAHRTGTTKMHEMGGLIFRMPFTFTAYLVAIISIAGIPPMSGFASKWLIYQQLVR
NGLPFLAFMAFFGSIGSFMYVFKPLAGIFLGQLKPQHKNIKEAPIPMLVSMMVLTLLTIFWGILPSNAISFTNKISEQVG
IATIKTTFSEIFASTGKWDSIIVVTVFTIGFVIAFVISALAKKPKKVDLMDTYTGGEFIYTAELYHFSYKMYRSFDRMFE
RWPLMEKWLENTSYKLRELGAFFRSLFYPSSPQGYVTLSILAILTAYWWLR
>Mature_610_residues
SLTVLLTISLISAPILYLLAKVNKNFAYTVYALIQLFAFSAIFFNWIYIPEHSGLRIFSNLELTFNASILNRFFASVTML
IISMTAVFMLSIKKKKEMKLMLLSLVSTGVIGSMLSNDFLTLLIFWELTTWSSLIMIVLDKKIAWKEALKYVAVGSIGTY
SMMYAVFLLYNRFGTLIYTDVANELMKTSGGFQLLIFILLAVMAFAKAGSFPLHIWLRGAHSSAPDEFSSILSGALTKMG
AFLMFIVVAVLPSTKFFAWMPEVQGIPLPNYILALLSGISIIVGTVMAIRMEDAKQLIAFSTVSNSGYILMALAIGGPYA
TAGGLMHVLNHALASSAMFLAIAAVAHRTGTTKMHEMGGLIFRMPFTFTAYLVAIISIAGIPPMSGFASKWLIYQQLVRN
GLPFLAFMAFFGSIGSFMYVFKPLAGIFLGQLKPQHKNIKEAPIPMLVSMMVLTLLTIFWGILPSNAISFTNKISEQVGI
ATIKTTFSEIFASTGKWDSIIVVTVFTIGFVIAFVISALAKKPKKVDLMDTYTGGEFIYTAELYHFSYKMYRSFDRMFER
WPLMEKWLENTSYKLRELGAFFRSLFYPSSPQGYVTLSILAILTAYWWLR

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=282, Percent_Identity=24.822695035461, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI251831116, Length=285, Percent_Identity=25.6140350877193, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788827, Length=409, Percent_Identity=30.8068459657702, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788831, Length=295, Percent_Identity=28.8135593220339, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI2367154, Length=341, Percent_Identity=29.3255131964809, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788613, Length=366, Percent_Identity=26.5027322404372, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788829, Length=363, Percent_Identity=24.7933884297521, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788614, Length=363, Percent_Identity=25.068870523416, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 68163; Mature: 68032

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTVLLTISLISAPILYLLAKVNKNFAYTVYALIQLFAFSAIFFNWIYIPEHSGLRIFS
CCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEE
NLELTFNASILNRFFASVTMLIISMTAVFMLSIKKKKEMKLMLLSLVSTGVIGSMLSNDF
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLLIFWELTTWSSLIMIVLDKKIAWKEALKYVAVGSIGTYSMMYAVFLLYNRFGTLIYT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHEEH
DVANELMKTSGGFQLLIFILLAVMAFAKAGSFPLHIWLRGAHSSAPDEFSSILSGALTKM
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
GAFLMFIVVAVLPSTKFFAWMPEVQGIPLPNYILALLSGISIIVGTVMAIRMEDAKQLIA
HHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTVSNSGYILMALAIGGPYATAGGLMHVLNHALASSAMFLAIAAVAHRTGTTKMHEMGG
HEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
LIFRMPFTFTAYLVAIISIAGIPPMSGFASKWLIYQQLVRNGLPFLAFMAFFGSIGSFMY
EEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VFKPLAGIFLGQLKPQHKNIKEAPIPMLVSMMVLTLLTIFWGILPSNAISFTNKISEQVG
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
IATIKTTFSEIFASTGKWDSIIVVTVFTIGFVIAFVISALAKKPKKVDLMDTYTGGEFIY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEE
TAELYHFSYKMYRSFDRMFERWPLMEKWLENTSYKLRELGAFFRSLFYPSSPQGYVTLSI
EEHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LAILTAYWWLR
HHHHHHHHHCC
>Mature Secondary Structure 
SLTVLLTISLISAPILYLLAKVNKNFAYTVYALIQLFAFSAIFFNWIYIPEHSGLRIFS
CEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEE
NLELTFNASILNRFFASVTMLIISMTAVFMLSIKKKKEMKLMLLSLVSTGVIGSMLSNDF
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLLIFWELTTWSSLIMIVLDKKIAWKEALKYVAVGSIGTYSMMYAVFLLYNRFGTLIYT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHEEH
DVANELMKTSGGFQLLIFILLAVMAFAKAGSFPLHIWLRGAHSSAPDEFSSILSGALTKM
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
GAFLMFIVVAVLPSTKFFAWMPEVQGIPLPNYILALLSGISIIVGTVMAIRMEDAKQLIA
HHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTVSNSGYILMALAIGGPYATAGGLMHVLNHALASSAMFLAIAAVAHRTGTTKMHEMGG
HEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
LIFRMPFTFTAYLVAIISIAGIPPMSGFASKWLIYQQLVRNGLPFLAFMAFFGSIGSFMY
EEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VFKPLAGIFLGQLKPQHKNIKEAPIPMLVSMMVLTLLTIFWGILPSNAISFTNKISEQVG
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
IATIKTTFSEIFASTGKWDSIIVVTVFTIGFVIAFVISALAKKPKKVDLMDTYTGGEFIY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEE
TAELYHFSYKMYRSFDRMFERWPLMEKWLENTSYKLRELGAFFRSLFYPSSPQGYVTLSI
EEHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LAILTAYWWLR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]