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The map label for this gene is fpoH [H]

Identifier: 239618415

GI number: 239618415

Start: 2184557

End: 2185438

Strand: Reverse

Name: fpoH [H]

Synonym: Kole_2055

Alternate gene names: 239618415

Gene position: 2185438-2184557 (Counterclockwise)

Preceding gene: 239618416

Following gene: 239618414

Centisome position: 94.93

GC content: 43.65

Gene sequence:

>882_bases
ATGACAGTGATAAAAAGTATTGGATTACTTTTATTAGCTTTCGTTTATACGATAACGCTGGAAGGTATTGCCAGAAAAAT
AGTTGCAAGGATAGAAAGAAGGTACGGCCCTCCTTTCTGGCAAAATTTCATCGATATCTTCAAAGCGCTGACAAAACATA
GTATCTCCCATGGGTTTATCTTCGATTTTGGTGTATGGATGGCGCTGGGTGGAACTATTGCAACTGTGCTATTCATTCCT
GCCGGGTCGCTCGTTGTATTCCCGGGGCTTGATAATATCTTCGTAGTTATATACGTGCTTGCCATAGGTCTTCTGGGTAT
GGCCATGTCGGCCGTGGGCTCTGGTAACCCCAACGCGTCGATAGGTATTGGGCGTGCTCTTACCCAAATGCTTGGTTACG
AGCTCCCCTTTCTTATAGTTATCCTCGGTATTCTGTTTGAGAACAGGACGTCATCGATCTCAGCAATCGTGGACAAGCAG
ATCGAAGCCGGAGTTTATAACGCATGGATAATGCCAATAGGGGCGGTTGTGGCTTTCATATCGCTAATGGGAATGTTGGG
CAAGAAACCCTTTGATTCTTTCATTGCACCTGCTGAAATCGCATCAGGTCCATTGGTAGAGTACAGTGGTAAATTCCTTG
GTTTGCTGATGATTCAACACGCATTTGCCGTGTTTATTGAGATATCGCTCTTTGTTAACTTCTTCCTTGGTGGTGGAAGA
ACCGTATGGGAATTTCTCGCAAAATTCTTTGTTGTCTTTTTCATTCTTGTAATTATTTCTGCTGTGCTACCAAGGTTCAG
AGTAGAGCAAGCAGTGAAATATTACTGGAAATGGCCGTTGATTTTGGCCTTCGTTCAAACGATCGTCGTTATCTTCTGGT
AA

Upstream 100 bases:

>100_bases
GCCTTTTTCCGTAGCCTCTTCTATCCTTCAAGCCCACAAGGCTATGTAACTTTGAGCATTCTTGCAATACTAACAGCCTA
TTGGTGGTTGAGGTGATAGC

Downstream 100 bases:

>100_bases
GGAGGTGCAATGAATGAACGCTGAAAAATCTGCTTGGAATAAAGTGGCAAACATGCTTCGTGCCCGCTCGTTATGGATGC
TTTATTACTGTACCGGATGC

Product: respiratory-chain NADH dehydrogenase subunit 1

Products: NAD+; ubiquinol

Alternate protein names: F(420)H(2) dehydrogenase subunit H; FPO subunit H [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTVIKSIGLLLLAFVYTITLEGIARKIVARIERRYGPPFWQNFIDIFKALTKHSISHGFIFDFGVWMALGGTIATVLFIP
AGSLVVFPGLDNIFVVIYVLAIGLLGMAMSAVGSGNPNASIGIGRALTQMLGYELPFLIVILGILFENRTSSISAIVDKQ
IEAGVYNAWIMPIGAVVAFISLMGMLGKKPFDSFIAPAEIASGPLVEYSGKFLGLLMIQHAFAVFIEISLFVNFFLGGGR
TVWEFLAKFFVVFFILVIISAVLPRFRVEQAVKYYWKWPLILAFVQTIVVIFW

Sequences:

>Translated_293_residues
MTVIKSIGLLLLAFVYTITLEGIARKIVARIERRYGPPFWQNFIDIFKALTKHSISHGFIFDFGVWMALGGTIATVLFIP
AGSLVVFPGLDNIFVVIYVLAIGLLGMAMSAVGSGNPNASIGIGRALTQMLGYELPFLIVILGILFENRTSSISAIVDKQ
IEAGVYNAWIMPIGAVVAFISLMGMLGKKPFDSFIAPAEIASGPLVEYSGKFLGLLMIQHAFAVFIEISLFVNFFLGGGR
TVWEFLAKFFVVFFILVIISAVLPRFRVEQAVKYYWKWPLILAFVQTIVVIFW
>Mature_292_residues
TVIKSIGLLLLAFVYTITLEGIARKIVARIERRYGPPFWQNFIDIFKALTKHSISHGFIFDFGVWMALGGTIATVLFIPA
GSLVVFPGLDNIFVVIYVLAIGLLGMAMSAVGSGNPNASIGIGRALTQMLGYELPFLIVILGILFENRTSSISAIVDKQI
EAGVYNAWIMPIGAVVAFISLMGMLGKKPFDSFIAPAEIASGPLVEYSGKFLGLLMIQHAFAVFIEISLFVNFFLGGGRT
VWEFLAKFFVVFFILVIISAVLPRFRVEQAVKYYWKWPLILAFVQTIVVIFW

Specific function: FPO shuttles electrons from F(420)H(2), via FAD and iron-sulfur (Fe-S) centers, to quinones in the F(420)H(2):heterodisulfide oxidoreduction chain. The immediate electron acceptor for the enzyme in this species is believed to be methanophenazine. Couples

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI87082115, Length=244, Percent_Identity=30.327868852459, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788618, Length=319, Percent_Identity=27.8996865203762, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789077, Length=249, Percent_Identity=29.3172690763052, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023021
- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.6.5.3

Molecular weight: Translated: 32378; Mature: 32247

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKSIGLLLLAFVYTITLEGIARKIVARIERRYGPPFWQNFIDIFKALTKHSISHGFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
FDFGVWMALGGTIATVLFIPAGSLVVFPGLDNIFVVIYVLAIGLLGMAMSAVGSGNPNAS
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGIGRALTQMLGYELPFLIVILGILFENRTSSISAIVDKQIEAGVYNAWIMPIGAVVAFI
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLMGMLGKKPFDSFIAPAEIASGPLVEYSGKFLGLLMIQHAFAVFIEISLFVNFFLGGGR
HHHHHHCCCCHHHHCCCHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TVWEFLAKFFVVFFILVIISAVLPRFRVEQAVKYYWKWPLILAFVQTIVVIFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVIKSIGLLLLAFVYTITLEGIARKIVARIERRYGPPFWQNFIDIFKALTKHSISHGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
FDFGVWMALGGTIATVLFIPAGSLVVFPGLDNIFVVIYVLAIGLLGMAMSAVGSGNPNAS
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IGIGRALTQMLGYELPFLIVILGILFENRTSSISAIVDKQIEAGVYNAWIMPIGAVVAFI
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLMGMLGKKPFDSFIAPAEIASGPLVEYSGKFLGLLMIQHAFAVFIEISLFVNFFLGGGR
HHHHHHCCCCHHHHCCCHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TVWEFLAKFFVVFFILVIISAVLPRFRVEQAVKYYWKWPLILAFVQTIVVIFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10751389; 12125824 [H]