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The map label for this gene is dut [H]

Identifier: 238028416

GI number: 238028416

Start: 3264038

End: 3264484

Strand: Direct

Name: dut [H]

Synonym: bglu_1g28800

Alternate gene names: 238028416

Gene position: 3264038-3264484 (Clockwise)

Preceding gene: 238028415

Following gene: 238028419

Centisome position: 83.55

GC content: 68.9

Gene sequence:

>447_bases
ATGAAACTCGACCTCAAGATCCTCGACGCGCGCATGCGCGACTACCTGCCGAACTACGCGACGCCGGGCAGCGCGGGCCT
CGACCTGCGCGCCTGCCTCGACGCCGCCGTCACGCTGCAGCCGGGCGAAACGACGCTGGTGCCGACCGGGCTCGCGATCC
ACCTCGCCGATGCCCGCTATGCGGCGCTGATCCTGCCGCGCTCGGGCCTCGGCCACAAGCACGGCATCGTGCTCGGCAAC
CTCGTCGGCCTGATTGACTCCGATTACCAGGGCCAGCTGATGATCTCGACCTGGAATCGCGGGCAGACCGCGTTCACGCT
CGAGCCGTTCGAGCGGCTCGCCCAGCTCGTGATCGTGCCGGTCGTGCAGGCGAGCTTCAACGTCGTCGAAGCGTTCGCAC
AGAGCGAGCGCGGTGAAGGCGGCTTCGGCAGCACCGGCCGTGGCTGA

Upstream 100 bases:

>100_bases
ACACGCGCCTGATCTGATCCATCCAAGCCGCCGGGCCGCAACGCATGGCGCGGCCCGGTGCATGTTGCAATTTCACGACT
CCTGCCAGCAGACCCGCCGC

Downstream 100 bases:

>100_bases
CGGCCCAAGGGCGACCAGAGCGCCCGCCGCAGGGACGTCGCGGGGGGCTCGCGGGGGCCGCCGCGATTAGTACGTTTATT
CCATTCGAATCACCGGCCGG

Product: Deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKLDLKILDARMRDYLPNYATPGSAGLDLRACLDAAVTLQPGETTLVPTGLAIHLADARYAALILPRSGLGHKHGIVLGN
LVGLIDSDYQGQLMISTWNRGQTAFTLEPFERLAQLVIVPVVQASFNVVEAFAQSERGEGGFGSTGRG

Sequences:

>Translated_148_residues
MKLDLKILDARMRDYLPNYATPGSAGLDLRACLDAAVTLQPGETTLVPTGLAIHLADARYAALILPRSGLGHKHGIVLGN
LVGLIDSDYQGQLMISTWNRGQTAFTLEPFERLAQLVIVPVVQASFNVVEAFAQSERGEGGFGSTGRG
>Mature_148_residues
MKLDLKILDARMRDYLPNYATPGSAGLDLRACLDAAVTLQPGETTLVPTGLAIHLADARYAALILPRSGLGHKHGIVLGN
LVGLIDSDYQGQLMISTWNRGQTAFTLEPFERLAQLVIVPVVQASFNVVEAFAQSERGEGGFGSTGRG

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=131, Percent_Identity=36.6412213740458, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI4503423, Length=131, Percent_Identity=36.6412213740458, Blast_Score=80, Evalue=6e-16,
Organism=Homo sapiens, GI70906441, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=72.2972972972973, Blast_Score=221, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71988561, Length=145, Percent_Identity=35.1724137931034, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319729, Length=145, Percent_Identity=35.1724137931034, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583610, Length=149, Percent_Identity=31.5436241610738, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921126, Length=149, Percent_Identity=31.5436241610738, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 15806; Mature: 15806

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDLKILDARMRDYLPNYATPGSAGLDLRACLDAAVTLQPGETTLVPTGLAIHLADARY
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCEEEEECCCCEEEECCCEEEEECCCCE
AALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISTWNRGQTAFTLEPFERLAQLVIVP
EEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHH
VVQASFNVVEAFAQSERGEGGFGSTGRG
HHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLDLKILDARMRDYLPNYATPGSAGLDLRACLDAAVTLQPGETTLVPTGLAIHLADARY
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCEEEEECCCCEEEECCCEEEEECCCCE
AALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISTWNRGQTAFTLEPFERLAQLVIVP
EEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHH
VVQASFNVVEAFAQSERGEGGFGSTGRG
HHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA