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The map label for this gene is purH [H]

Identifier: 229917954

GI number: 229917954

Start: 2180595

End: 2182115

Strand: Direct

Name: purH [H]

Synonym: EAT1b_2233

Alternate gene names: 229917954

Gene position: 2180595-2182115 (Clockwise)

Preceding gene: 229917953

Following gene: 229917955

Centisome position: 72.69

GC content: 52.66

Gene sequence:

>1521_bases
GTGAGAGCATTATTAAGTGTTTCTGATAAAACGGGAATTGTCGAACTCGCGACCGTCTTGCATGAGGCGGGCATCGAGCT
CATTTCAACAGGTGGGACCGAGGCTGCGCTTCAGCAGGCGGGATTGCCAGTCAAGAACATTTCGGAAGTGACATCGTTCC
CTGAAATGTTAGACGGCCGTGTCAAAACGTTGCACCCACTCGTTCACGGGGGCTTGCTCGGACGTCGCGACCTCGATACG
CATGTCGCACAAATGAAAGATCATCACATCGAACCAATCGATTATGTCGTGGTCAATCTGTATCCGTTCAAGGAGACGAT
TGCGAAAGAGAATACGACATACGAAGAAGCGATTGAAAACATTGATATTGGTGGACCGAGCATGTTGCGTTCGGCTGCGA
AAAACCATGCGAGTGTCACCGTCGTCGTGGACCCGAGTGATTATACACTCGTCGCCGAGGAAGTACGTGGAGGCGGTACA
TCGTTTGAAACACGTCAACGTCTCGCGACGAAAGCGTTCCGACATACGGCTGCCTATGACGCGTTGATTGCCGACTACTT
AGGTCGTCAAATCGGCGAAACGTTCCCAGAGCAGTTGACGGTCACGTATGAAAAAGTTCAAGACCTTCGTTACGGAGAGA
ACCCACACCAACAAGCGGCGTTTTATAAGACACCGTTATATGACGGGATGACACTTGCGAAGGCGACACAGCTTCACGGG
AAAGAGCTCTCGTATAACAACATTCAAGATACGAATGCAGCCCTTGAAGCACTCAGTGAATTGACTGAGCCGGCGGCAGT
CGTCGTCAAGCACATGAACCCGTGCGGCGTTGCAGTCGGACGGACGATTTCTCAAGCGTTCGAACGTGCCCATGCGGCCG
ACCCGGTCTCCATCTTTGGTGGAATCGTCGCCTTAAACCGTGAAGTCGATCTTGAGACGGCAGATGCCCTTCGCAACATC
TTTTTAGAGATCATCATTGCCCCATCCTTTAGTGAGGCAGCACTTGACCGATTGAAAGAGAAAAAGAATCTTCGGTTGTT
GACGTTAGATATGGGACAGATGACAGGCATGCGCCTTACGGCTGTCGCCGGAGGATTGCTCGTACAAGACGGGGATGACA
TGACACTCGATGATGCCTCATGCCAAATCGTGACGGATCGTCGCCCGACAGCAGCGGAATGGGAAGACTTGAAACTTGCA
TGGCGTGTCGTCAAGCACGTCAAATCGAACGCGATTGTCGTCGCAAAAGACGAGACGACGATTGGAATCGGTGCCGGACA
GATGAACCGCGTCGGTGCAGCGAAAATCGCTTTTGAACAAGCCGGGGAACGCGCGTCAGGCGCGGCTCTCGGAAGTGATG
CCTTCTTCCCGATGGCAGATACGGTCGAAGCGGCAGCGGCTGCAGGAATCACGGCCATCATCCAACCGGGTGGGTCGATT
CGCGATGCCGAATCGATTGAAGCGGCAAATCGCCATGGCATCACGATGGTATTCACATCAGTCAGACATTTCAAACACTG
A

Upstream 100 bases:

>100_bases
GGTGTGACCGCACCGAGGCGACGCGTCGCATTCAGACGATCGAGCACCAACTGTATCCGCGCGTATTACAACAAGTGTTG
AATCAACAGGAGGTTTTCAA

Downstream 100 bases:

>100_bases
GAGGGGTTTGCGTCATGAACGTATTGGTCATCGGTCGAGGAGGGCGCGAGCATGCGCTCGCATGGAAGTTGAAACAAGAC
GGGCATCACGTCTTCGTTGC

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MRALLSVSDKTGIVELATVLHEAGIELISTGGTEAALQQAGLPVKNISEVTSFPEMLDGRVKTLHPLVHGGLLGRRDLDT
HVAQMKDHHIEPIDYVVVNLYPFKETIAKENTTYEEAIENIDIGGPSMLRSAAKNHASVTVVVDPSDYTLVAEEVRGGGT
SFETRQRLATKAFRHTAAYDALIADYLGRQIGETFPEQLTVTYEKVQDLRYGENPHQQAAFYKTPLYDGMTLAKATQLHG
KELSYNNIQDTNAALEALSELTEPAAVVVKHMNPCGVAVGRTISQAFERAHAADPVSIFGGIVALNREVDLETADALRNI
FLEIIIAPSFSEAALDRLKEKKNLRLLTLDMGQMTGMRLTAVAGGLLVQDGDDMTLDDASCQIVTDRRPTAAEWEDLKLA
WRVVKHVKSNAIVVAKDETTIGIGAGQMNRVGAAKIAFEQAGERASGAALGSDAFFPMADTVEAAAAAGITAIIQPGGSI
RDAESIEAANRHGITMVFTSVRHFKH

Sequences:

>Translated_506_residues
MRALLSVSDKTGIVELATVLHEAGIELISTGGTEAALQQAGLPVKNISEVTSFPEMLDGRVKTLHPLVHGGLLGRRDLDT
HVAQMKDHHIEPIDYVVVNLYPFKETIAKENTTYEEAIENIDIGGPSMLRSAAKNHASVTVVVDPSDYTLVAEEVRGGGT
SFETRQRLATKAFRHTAAYDALIADYLGRQIGETFPEQLTVTYEKVQDLRYGENPHQQAAFYKTPLYDGMTLAKATQLHG
KELSYNNIQDTNAALEALSELTEPAAVVVKHMNPCGVAVGRTISQAFERAHAADPVSIFGGIVALNREVDLETADALRNI
FLEIIIAPSFSEAALDRLKEKKNLRLLTLDMGQMTGMRLTAVAGGLLVQDGDDMTLDDASCQIVTDRRPTAAEWEDLKLA
WRVVKHVKSNAIVVAKDETTIGIGAGQMNRVGAAKIAFEQAGERASGAALGSDAFFPMADTVEAAAAAGITAIIQPGGSI
RDAESIEAANRHGITMVFTSVRHFKH
>Mature_506_residues
MRALLSVSDKTGIVELATVLHEAGIELISTGGTEAALQQAGLPVKNISEVTSFPEMLDGRVKTLHPLVHGGLLGRRDLDT
HVAQMKDHHIEPIDYVVVNLYPFKETIAKENTTYEEAIENIDIGGPSMLRSAAKNHASVTVVVDPSDYTLVAEEVRGGGT
SFETRQRLATKAFRHTAAYDALIADYLGRQIGETFPEQLTVTYEKVQDLRYGENPHQQAAFYKTPLYDGMTLAKATQLHG
KELSYNNIQDTNAALEALSELTEPAAVVVKHMNPCGVAVGRTISQAFERAHAADPVSIFGGIVALNREVDLETADALRNI
FLEIIIAPSFSEAALDRLKEKKNLRLLTLDMGQMTGMRLTAVAGGLLVQDGDDMTLDDASCQIVTDRRPTAAEWEDLKLA
WRVVKHVKSNAIVVAKDETTIGIGAGQMNRVGAAKIAFEQAGERASGAALGSDAFFPMADTVEAAAAAGITAIIQPGGSI
RDAESIEAANRHGITMVFTSVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=472, Percent_Identity=40.0423728813559, Blast_Score=279, Evalue=5e-75,
Organism=Escherichia coli, GI1790439, Length=523, Percent_Identity=54.1108986615679, Blast_Score=536, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI71985564, Length=470, Percent_Identity=34.8936170212766, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=306, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=468, Percent_Identity=37.3931623931624, Blast_Score=280, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6323056, Length=468, Percent_Identity=37.1794871794872, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24649832, Length=469, Percent_Identity=38.8059701492537, Blast_Score=275, Evalue=4e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54621; Mature: 54621

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALLSVSDKTGIVELATVLHEAGIELISTGGTEAALQQAGLPVKNISEVTSFPEMLDGR
CCCEEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHCCH
VKTLHPLVHGGLLGRRDLDTHVAQMKDHHIEPIDYVVVNLYPFKETIAKENTTYEEAIEN
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHCCCCCHHHHHHH
IDIGGPSMLRSAAKNHASVTVVVDPSDYTLVAEEVRGGGTSFETRQRLATKAFRHTAAYD
CCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALIADYLGRQIGETFPEQLTVTYEKVQDLRYGENPHQQAAFYKTPLYDGMTLAKATQLHG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCCCCCHHHHHHHHCC
KELSYNNIQDTNAALEALSELTEPAAVVVKHMNPCGVAVGRTISQAFERAHAADPVSIFG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
GIVALNREVDLETADALRNIFLEIIIAPSFSEAALDRLKEKKNLRLLTLDMGQMTGMRLT
HHHEECCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEE
AVAGGLLVQDGDDMTLDDASCQIVTDRRPTAAEWEDLKLAWRVVKHVKSNAIVVAKDETT
HECCCEEEECCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCE
IGIGAGQMNRVGAAKIAFEQAGERASGAALGSDAFFPMADTVEAAAAAGITAIIQPGGSI
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCC
RDAESIEAANRHGITMVFTSVRHFKH
CCHHHHHHHCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MRALLSVSDKTGIVELATVLHEAGIELISTGGTEAALQQAGLPVKNISEVTSFPEMLDGR
CCCEEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHCCH
VKTLHPLVHGGLLGRRDLDTHVAQMKDHHIEPIDYVVVNLYPFKETIAKENTTYEEAIEN
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHCCCCCHHHHHHH
IDIGGPSMLRSAAKNHASVTVVVDPSDYTLVAEEVRGGGTSFETRQRLATKAFRHTAAYD
CCCCCHHHHHHHHCCCCEEEEEECCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALIADYLGRQIGETFPEQLTVTYEKVQDLRYGENPHQQAAFYKTPLYDGMTLAKATQLHG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHEEECCCCCCCHHHHHHHHCC
KELSYNNIQDTNAALEALSELTEPAAVVVKHMNPCGVAVGRTISQAFERAHAADPVSIFG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
GIVALNREVDLETADALRNIFLEIIIAPSFSEAALDRLKEKKNLRLLTLDMGQMTGMRLT
HHHEECCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEE
AVAGGLLVQDGDDMTLDDASCQIVTDRRPTAAEWEDLKLAWRVVKHVKSNAIVVAKDETT
HECCCEEEECCCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCE
IGIGAGQMNRVGAAKIAFEQAGERASGAALGSDAFFPMADTVEAAAAAGITAIIQPGGSI
EEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCC
RDAESIEAANRHGITMVFTSVRHFKH
CCHHHHHHHCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]