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The map label for this gene is purD [H]

Identifier: 229917955

GI number: 229917955

Start: 2182130

End: 2183383

Strand: Direct

Name: purD [H]

Synonym: EAT1b_2234

Alternate gene names: 229917955

Gene position: 2182130-2183383 (Clockwise)

Preceding gene: 229917954

Following gene: 229917956

Centisome position: 72.74

GC content: 55.18

Gene sequence:

>1254_bases
ATGAACGTATTGGTCATCGGTCGAGGAGGGCGCGAGCATGCGCTCGCATGGAAGTTGAAACAAGACGGGCATCACGTCTT
CGTTGCACCGGGCAACGACTTCATGGAAGGGATGACGTGCGCGTCGATCCATGAAACAGACGTGGAGGCGCTCGCCCATT
TCGCAGAAGAGAACGAAATCGAATGGACGATTGTCGGACCGGAGTCGTCGCTCATGGCAGGAGTCGTGGACACGTTCCGC
GACAAAGGGCTGAACATCTTTGGACCGACGAAAGCGGCTGCCTTACTCGAGGGAAGCAAGGCATTCGCCAAAGAAGTCAT
GATGGAAGCCGGAATTCCGACAGCGGCATACGAGGCGTTCACGGATGCGGCATCGGCGCTTGCCTATATCGAGAAGCTCG
GTGCACCACTCGTCGTCAAGGCGGACGGATTGGCTGCCGGTAAAGGCGTCACGGTTGCGTTCACGGTCGAAGAGGCAGAA
GCTGCCGTACGTGATATTTTCACGACGGATAAATTTGGCCAACAGTCACAAGTCGTCATCGAGGAGTTCCTCGATGGGGA
AGAGTTTTCGCTCATGGCATTCGTCTCGGGAGAGCGTGTCATTCCGATGATCACCTCGCAAGATCATAAACGTGCCTATG
ACGGCGACGGGGGACCAAACACGGGTGGGATGGGCGCATATAGCCCGATGCCGCATCTTTCAAAGGACGTGTATGAGGAG
GCCGTCGAGACCGTATTAAAACCGCTCGCACAGACGATGGTCAAGCGAGGTACTCCATTTGAAGGATTCTTGTACGCCGG
TCTCATCTTGACGGCAAATGGACCAAAAGTCATCGAATTCAACGCACGCTTCGGTGACCCGGAAACGGAGGTCATCTTGC
CGAACTTGAAATCGCCGCTCCTTGACGTGATTGAAGCGGTCGTTCGCCAAGAATCGTATTCGGTTGACTGGATTGACGGA
TACACGATGGGTGTCGTCATTGCGGCCGAAGGGTATCCGGACCGACCGATCACGGGCCAGGTGTTGTCAGGATTTGAGTC
ACTTCCGAAAGATGTGCTAGTCTTCCATGCCGGCACGACAAAGAAGGGCGATCAAGTCGTAGGGAGCGGAGGTCGTCTAC
TCGTTGTGACGGCGACGAAGCCGACACTGCTTGAGGCGAAAACGGCTGTGTATGCGGCAATTTCACAACTCGATCTACCT
GAAACGTTCCACCGAACGGACATCGGGTCACGGGCGTTCACACAGATTATTTGA

Upstream 100 bases:

>100_bases
CGATTCGCGATGCCGAATCGATTGAAGCGGCAAATCGCCATGGCATCACGATGGTATTCACATCAGTCAGACATTTCAAA
CACTGAGAGGGGTTTGCGTC

Downstream 100 bases:

>100_bases
AGGATGGGTCAACCCATCCTTTTTTTCATGCGAAAAAAAGGAAATGCCGATATCCTGTTCTACAATAATAAAGTAGCTTG
AAGAAGGAGGGTGGTCGGCC

Product: phosphoribosylamine/glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNVLVIGRGGREHALAWKLKQDGHHVFVAPGNDFMEGMTCASIHETDVEALAHFAEENEIEWTIVGPESSLMAGVVDTFR
DKGLNIFGPTKAAALLEGSKAFAKEVMMEAGIPTAAYEAFTDAASALAYIEKLGAPLVVKADGLAAGKGVTVAFTVEEAE
AAVRDIFTTDKFGQQSQVVIEEFLDGEEFSLMAFVSGERVIPMITSQDHKRAYDGDGGPNTGGMGAYSPMPHLSKDVYEE
AVETVLKPLAQTMVKRGTPFEGFLYAGLILTANGPKVIEFNARFGDPETEVILPNLKSPLLDVIEAVVRQESYSVDWIDG
YTMGVVIAAEGYPDRPITGQVLSGFESLPKDVLVFHAGTTKKGDQVVGSGGRLLVVTATKPTLLEAKTAVYAAISQLDLP
ETFHRTDIGSRAFTQII

Sequences:

>Translated_417_residues
MNVLVIGRGGREHALAWKLKQDGHHVFVAPGNDFMEGMTCASIHETDVEALAHFAEENEIEWTIVGPESSLMAGVVDTFR
DKGLNIFGPTKAAALLEGSKAFAKEVMMEAGIPTAAYEAFTDAASALAYIEKLGAPLVVKADGLAAGKGVTVAFTVEEAE
AAVRDIFTTDKFGQQSQVVIEEFLDGEEFSLMAFVSGERVIPMITSQDHKRAYDGDGGPNTGGMGAYSPMPHLSKDVYEE
AVETVLKPLAQTMVKRGTPFEGFLYAGLILTANGPKVIEFNARFGDPETEVILPNLKSPLLDVIEAVVRQESYSVDWIDG
YTMGVVIAAEGYPDRPITGQVLSGFESLPKDVLVFHAGTTKKGDQVVGSGGRLLVVTATKPTLLEAKTAVYAAISQLDLP
ETFHRTDIGSRAFTQII
>Mature_417_residues
MNVLVIGRGGREHALAWKLKQDGHHVFVAPGNDFMEGMTCASIHETDVEALAHFAEENEIEWTIVGPESSLMAGVVDTFR
DKGLNIFGPTKAAALLEGSKAFAKEVMMEAGIPTAAYEAFTDAASALAYIEKLGAPLVVKADGLAAGKGVTVAFTVEEAE
AAVRDIFTTDKFGQQSQVVIEEFLDGEEFSLMAFVSGERVIPMITSQDHKRAYDGDGGPNTGGMGAYSPMPHLSKDVYEE
AVETVLKPLAQTMVKRGTPFEGFLYAGLILTANGPKVIEFNARFGDPETEVILPNLKSPLLDVIEAVVRQESYSVDWIDG
YTMGVVIAAEGYPDRPITGQVLSGFESLPKDVLVFHAGTTKKGDQVVGSGGRLLVVTATKPTLLEAKTAVYAAISQLDLP
ETFHRTDIGSRAFTQII

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=47.877358490566, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=47.877358490566, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=47.877358490566, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=47.877358490566, Blast_Score=357, Evalue=1e-98,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=50.8158508158508, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=38.7173396674584, Blast_Score=247, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=37.5854214123007, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=42.1911421911422, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=42.1911421911422, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44720; Mature: 44720

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGRGGREHALAWKLKQDGHHVFVAPGNDFMEGMTCASIHETDVEALAHFAEENEI
CEEEEEECCCCCCEEEEEEECCCCEEEEECCCHHHHCCEECHHHHHHHHHHHHHHCCCCE
EWTIVGPESSLMAGVVDTFRDKGLNIFGPTKAAALLEGSKAFAKEVMMEAGIPTAAYEAF
EEEEECCCHHHHHHHHHHHHHCCCEEECCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHH
TDAASALAYIEKLGAPLVVKADGLAAGKGVTVAFTVEEAEAAVRDIFTTDKFGQQSQVVI
HHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCHHHHH
EEFLDGEEFSLMAFVSGERVIPMITSQDHKRAYDGDGGPNTGGMGAYSPMPHLSKDVYEE
HHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AVETVLKPLAQTMVKRGTPFEGFLYAGLILTANGPKVIEFNARFGDPETEVILPNLKSPL
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEECCCCCCHH
LDVIEAVVRQESYSVDWIDGYTMGVVIAAEGYPDRPITGQVLSGFESLPKDVLVFHAGTT
HHHHHHHHHHCCCCEEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCC
KKGDQVVGSGGRLLVVTATKPTLLEAKTAVYAAISQLDLPETFHRTDIGSRAFTQII
CCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHCC
>Mature Secondary Structure
MNVLVIGRGGREHALAWKLKQDGHHVFVAPGNDFMEGMTCASIHETDVEALAHFAEENEI
CEEEEEECCCCCCEEEEEEECCCCEEEEECCCHHHHCCEECHHHHHHHHHHHHHHCCCCE
EWTIVGPESSLMAGVVDTFRDKGLNIFGPTKAAALLEGSKAFAKEVMMEAGIPTAAYEAF
EEEEECCCHHHHHHHHHHHHHCCCEEECCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHH
TDAASALAYIEKLGAPLVVKADGLAAGKGVTVAFTVEEAEAAVRDIFTTDKFGQQSQVVI
HHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCHHHHH
EEFLDGEEFSLMAFVSGERVIPMITSQDHKRAYDGDGGPNTGGMGAYSPMPHLSKDVYEE
HHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AVETVLKPLAQTMVKRGTPFEGFLYAGLILTANGPKVIEFNARFGDPETEVILPNLKSPL
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEECCCCCCHH
LDVIEAVVRQESYSVDWIDGYTMGVVIAAEGYPDRPITGQVLSGFESLPKDVLVFHAGTT
HHHHHHHHHHCCCCEEEECCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCC
KKGDQVVGSGGRLLVVTATKPTLLEAKTAVYAAISQLDLPETFHRTDIGSRAFTQII
CCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]