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The map label for this gene is purN [H]

Identifier: 229917953

GI number: 229917953

Start: 2180020

End: 2180598

Strand: Direct

Name: purN [H]

Synonym: EAT1b_2232

Alternate gene names: 229917953

Gene position: 2180020-2180598 (Clockwise)

Preceding gene: 229917952

Following gene: 229917954

Centisome position: 72.67

GC content: 51.99

Gene sequence:

>579_bases
ATGAAACGATTTGCCATCTTTGCGTCAGGATCGGGAAGTAACGCCGAAGCGATCTGGCAAGCGATCGCCGATGGTCAATT
GTCTGCCGAATGTGTGTTGCTCGTGACGGACAAACCAGAAGCCACTGTTCTCGATCGAGCGGAACGTTATGGGATCTCAA
GCTTTTCGTTTACACCGAAGGCGTACGCGTCAAAAGAAGAGTTCGAGGAAGAGATCCTCGTCCTGCTCCGTACGCTTCGT
GTAGACTATATCGTGTTGGCGGGGTATATGCGCTTGATCGGAAATGTGTTACTATCCGCATATCCGAATCGCATTCTAAA
CATTCATCCATCGCTTTTACCAGCTTTTCCGGGTAAAGACGCAATCGGACAGGCGCTTGACGCCAACGTGCCGACGAGTG
GTGTGACCGTTCATTATGTGGATGCGGGGATGGACACGGGTCCGATCATCGCACAGGCGTCTGTCGAGATTGAAGGGTGT
GACCGCACCGAGGCGACGCGTCGCATTCAGACGATCGAGCACCAACTGTATCCGCGCGTATTACAACAAGTGTTGAATCA
ACAGGAGGTTTTCAAGTGA

Upstream 100 bases:

>100_bases
TGGAACTCGTCGAAGCGGCGCTTGAACGTGTCGGGGAGACTGCGCACCGAATCGGGAAAGTCGAGAGTCGTCCAGGCATC
CGGATTTCAGGAGTGGACCA

Downstream 100 bases:

>100_bases
GAGCATTATTAAGTGTTTCTGATAAAACGGGAATTGTCGAACTCGCGACCGTCTTGCATGAGGCGGGCATCGAGCTCATT
TCAACAGGTGGGACCGAGGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKRFAIFASGSGSNAEAIWQAIADGQLSAECVLLVTDKPEATVLDRAERYGISSFSFTPKAYASKEEFEEEILVLLRTLR
VDYIVLAGYMRLIGNVLLSAYPNRILNIHPSLLPAFPGKDAIGQALDANVPTSGVTVHYVDAGMDTGPIIAQASVEIEGC
DRTEATRRIQTIEHQLYPRVLQQVLNQQEVFK

Sequences:

>Translated_192_residues
MKRFAIFASGSGSNAEAIWQAIADGQLSAECVLLVTDKPEATVLDRAERYGISSFSFTPKAYASKEEFEEEILVLLRTLR
VDYIVLAGYMRLIGNVLLSAYPNRILNIHPSLLPAFPGKDAIGQALDANVPTSGVTVHYVDAGMDTGPIIAQASVEIEGC
DRTEATRRIQTIEHQLYPRVLQQVLNQQEVFK
>Mature_192_residues
MKRFAIFASGSGSNAEAIWQAIADGQLSAECVLLVTDKPEATVLDRAERYGISSFSFTPKAYASKEEFEEEILVLLRTLR
VDYIVLAGYMRLIGNVLLSAYPNRILNIHPSLLPAFPGKDAIGQALDANVPTSGVTVHYVDAGMDTGPIIAQASVEIEGC
DRTEATRRIQTIEHQLYPRVLQQVLNQQEVFK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=183, Percent_Identity=35.5191256830601, Blast_Score=127, Evalue=5e-30,
Organism=Homo sapiens, GI209869995, Length=183, Percent_Identity=35.5191256830601, Blast_Score=127, Evalue=5e-30,
Organism=Homo sapiens, GI209869993, Length=183, Percent_Identity=35.5191256830601, Blast_Score=127, Evalue=5e-30,
Organism=Escherichia coli, GI1788846, Length=177, Percent_Identity=36.1581920903955, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1787483, Length=142, Percent_Identity=33.0985915492958, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=36.6666666666667, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320616, Length=212, Percent_Identity=30.6603773584906, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=41.4893617021277, Blast_Score=140, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21126; Mature: 21126

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFAIFASGSGSNAEAIWQAIADGQLSAECVLLVTDKPEATVLDRAERYGISSFSFTPK
CCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCH
AYASKEEFEEEILVLLRTLRVDYIVLAGYMRLIGNVLLSAYPNRILNIHPSLLPAFPGKD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCH
AIGQALDANVPTSGVTVHYVDAGMDTGPIIAQASVEIEGCDRTEATRRIQTIEHQLYPRV
HHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LQQVLNQQEVFK
HHHHHHHHHCCC
>Mature Secondary Structure
MKRFAIFASGSGSNAEAIWQAIADGQLSAECVLLVTDKPEATVLDRAERYGISSFSFTPK
CCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCH
AYASKEEFEEEILVLLRTLRVDYIVLAGYMRLIGNVLLSAYPNRILNIHPSLLPAFPGKD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCH
AIGQALDANVPTSGVTVHYVDAGMDTGPIIAQASVEIEGCDRTEATRRIQTIEHQLYPRV
HHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LQQVLNQQEVFK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]