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The map label for this gene is pgk

Identifier: 229916591

GI number: 229916591

Start: 816628

End: 817812

Strand: Reverse

Name: pgk

Synonym: EAT1b_0863

Alternate gene names: 229916591

Gene position: 817812-816628 (Counterclockwise)

Preceding gene: 229916592

Following gene: 229916590

Centisome position: 27.26

GC content: 52.15

Gene sequence:

>1185_bases
ATGAACAAACAGTCTATTCGTGACATCGATGTAAAAGGGAAACGCGTCTTTTGCCGCGTTGACTTCAACGTTCCACTCAA
AGATGGCGTCATCCAAGACGAAACTCGGATCCAAGCCGCACTTCCAACGATCAAGCACTTGCTTGACGGTGGTGCGAAAC
TCGTCCTTGCGAGCCACCTCGGCCGTCCAAAAGGCGAGAAGAAACCTGAGTTCTCACTTGCTCCGGTAGCGAAGCGTCTC
GCTGAGCTCCTCGGAAAAGATGTACCGCTCGTCGAAGAAGCATACGGTCCGGTCGCAGAAGAAGCGGTCGCTAACCTCTC
TGAAGGCGATGTCGTCGTTTTAGAGAACGTTCGTTTCTACCCAGGTGAAACGAAAAACGACCCTGAACTTGCAAAAGGAT
TTGCAGCACTTGCTGACGTATACGTCAACGACGCGTTCGGCGCGGCTCACCGTGCACACGCTTCAACTGAAGGGATTGCA
CACCACGTGGACACAGCCGTTGCTGGTCTCTTGATGGAAAAAGAACTTGAAGTTCTCGGTAAAGCTCTTTCAAACCCAGA
CCGTCCGTTCACGGCGATTATCGGCGGGTCGAAAGTAGCGGATAAGATTGGGGTCATCGACCACCTTCTTGACATCGTTG
ACACACTCATCATCGGTGGCGGACTCTCGTACACGTTCTCGAAAGCACTCGGGCACGAAGTCGGAACGTCACTCCTTGAA
GAGGATAAGCTTGACCTCGCTCGTCAATTCATGAAGAAAGCAGAAGACAAAGGCGTGAAATTCTTGATGCCTGTCGACTG
CGTCATCACGAAAGAATTTGGCGAAGAAACGTATGTCGGGCCAGTCGATATCGACTCGATCCCAGCGGATCACATGGGTC
TCGATATCGGACCGAAGACAGTCGAACTTTACGCGGACGCGATTCGCGAATCGAAACTTGTCGTATGGAACGGACCGATG
GGCGTATTCGAACTCGATAAATACGCAAACGGAACGAAAGGTGTCGCTCAAGCGCTTGCGGACAGCGATGCATACTCAAT
CATCGGTGGTGGCGACTCAGCAGCTGCGGCAGCCAAGTTTGGTCTTGCTGACAAGATGAGTCACATCTCAACTGGTGGCG
GCGCGTCACTCGAATTCATGGAAGGCAAAAAACTTCCAGGTGTCGAGGCGTTGAACGACAAGTAA

Upstream 100 bases:

>100_bases
TAATTTCGTGAACTAAATAGAATCAAATAGGAGCGGAAACAAGAGATTGTTCTCCGCTCTATTTATGAAATGACACGCAC
GACATATGGAGGCGAAAGTT

Downstream 100 bases:

>100_bases
TTTAGAGGAGGGTTATCGATGCGTAAACCGATTATTGCAGGTAACTGGAAAATGAACAAAACACTCTCGGAAGCTGTTGC
TTTCGTTGAGGAAGTAAAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKQSIRDIDVKGKRVFCRVDFNVPLKDGVIQDETRIQAALPTIKHLLDGGAKLVLASHLGRPKGEKKPEFSLAPVAKRL
AELLGKDVPLVEEAYGPVAEEAVANLSEGDVVVLENVRFYPGETKNDPELAKGFAALADVYVNDAFGAAHRAHASTEGIA
HHVDTAVAGLLMEKELEVLGKALSNPDRPFTAIIGGSKVADKIGVIDHLLDIVDTLIIGGGLSYTFSKALGHEVGTSLLE
EDKLDLARQFMKKAEDKGVKFLMPVDCVITKEFGEETYVGPVDIDSIPADHMGLDIGPKTVELYADAIRESKLVVWNGPM
GVFELDKYANGTKGVAQALADSDAYSIIGGGDSAAAAAKFGLADKMSHISTGGGASLEFMEGKKLPGVEALNDK

Sequences:

>Translated_394_residues
MNKQSIRDIDVKGKRVFCRVDFNVPLKDGVIQDETRIQAALPTIKHLLDGGAKLVLASHLGRPKGEKKPEFSLAPVAKRL
AELLGKDVPLVEEAYGPVAEEAVANLSEGDVVVLENVRFYPGETKNDPELAKGFAALADVYVNDAFGAAHRAHASTEGIA
HHVDTAVAGLLMEKELEVLGKALSNPDRPFTAIIGGSKVADKIGVIDHLLDIVDTLIIGGGLSYTFSKALGHEVGTSLLE
EDKLDLARQFMKKAEDKGVKFLMPVDCVITKEFGEETYVGPVDIDSIPADHMGLDIGPKTVELYADAIRESKLVVWNGPM
GVFELDKYANGTKGVAQALADSDAYSIIGGGDSAAAAAKFGLADKMSHISTGGGASLEFMEGKKLPGVEALNDK
>Mature_394_residues
MNKQSIRDIDVKGKRVFCRVDFNVPLKDGVIQDETRIQAALPTIKHLLDGGAKLVLASHLGRPKGEKKPEFSLAPVAKRL
AELLGKDVPLVEEAYGPVAEEAVANLSEGDVVVLENVRFYPGETKNDPELAKGFAALADVYVNDAFGAAHRAHASTEGIA
HHVDTAVAGLLMEKELEVLGKALSNPDRPFTAIIGGSKVADKIGVIDHLLDIVDTLIIGGGLSYTFSKALGHEVGTSLLE
EDKLDLARQFMKKAEDKGVKFLMPVDCVITKEFGEETYVGPVDIDSIPADHMGLDIGPKTVELYADAIRESKLVVWNGPM
GVFELDKYANGTKGVAQALADSDAYSIIGGGDSAAAAAKFGLADKMSHISTGGGASLEFMEGKKLPGVEALNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=48.5507246376812, Blast_Score=370, Evalue=1e-103,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=47.2154963680387, Blast_Score=357, Evalue=1e-98,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=47.8481012658228, Blast_Score=347, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=48.0676328502416, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=48.6682808716707, Blast_Score=365, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=48.0582524271845, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=47.9518072289157, Blast_Score=348, Evalue=4e-96,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_EXISA (C4L5H7)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885237.1
- GeneID:   7869028
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0863
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41898; Mature: 41898

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 202-202 BINDING 293-293 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQSIRDIDVKGKRVFCRVDFNVPLKDGVIQDETRIQAALPTIKHLLDGGAKLVLASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEHHHHC
GRPKGEKKPEFSLAPVAKRLAELLGKDVPLVEEAYGPVAEEAVANLSEGDVVVLENVRFY
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHCCCCCCEEEEECEEEC
PGETKNDPELAKGFAALADVYVNDAFGAAHRAHASTEGIAHHVDTAVAGLLMEKELEVLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KALSNPDRPFTAIIGGSKVADKIGVIDHLLDIVDTLIIGGGLSYTFSKALGHEVGTSLLE
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EDKLDLARQFMKKAEDKGVKFLMPVDCVITKEFGEETYVGPVDIDSIPADHMGLDIGPKT
HHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCHH
VELYADAIRESKLVVWNGPMGVFELDKYANGTKGVAQALADSDAYSIIGGGDSAAAAAKF
HHHHHHHHHCCCEEEEECCCCCEEHHHHCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHH
GLADKMSHISTGGGASLEFMEGKKLPGVEALNDK
CHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNKQSIRDIDVKGKRVFCRVDFNVPLKDGVIQDETRIQAALPTIKHLLDGGAKLVLASHL
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEHHHHC
GRPKGEKKPEFSLAPVAKRLAELLGKDVPLVEEAYGPVAEEAVANLSEGDVVVLENVRFY
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHCCCCCCEEEEECEEEC
PGETKNDPELAKGFAALADVYVNDAFGAAHRAHASTEGIAHHVDTAVAGLLMEKELEVLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
KALSNPDRPFTAIIGGSKVADKIGVIDHLLDIVDTLIIGGGLSYTFSKALGHEVGTSLLE
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EDKLDLARQFMKKAEDKGVKFLMPVDCVITKEFGEETYVGPVDIDSIPADHMGLDIGPKT
HHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCCHH
VELYADAIRESKLVVWNGPMGVFELDKYANGTKGVAQALADSDAYSIIGGGDSAAAAAKF
HHHHHHHHHCCCEEEEECCCCCEEHHHHCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHH
GLADKMSHISTGGGASLEFMEGKKLPGVEALNDK
CHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA