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The map label for this gene is mhpC [H]

Identifier: 222524127

GI number: 222524127

Start: 1061871

End: 1062713

Strand: Direct

Name: mhpC [H]

Synonym: Chy400_0843

Alternate gene names: 222524127

Gene position: 1061871-1062713 (Clockwise)

Preceding gene: 222524121

Following gene: 222524129

Centisome position: 20.15

GC content: 65.48

Gene sequence:

>843_bases
ATGCGTTCACGCTGGTTACGGATCAACGGCTATCAACTCCACTGGATCGAAGCCGGACAGGGGCCGGCTGTAATCCTCTT
ACACGGTTTCGCCGGCTCGTGTGCCGACTGGGAACCAACTGTAGACTGGCTGGCTCGACAGGGGTATCGCGCCTTAGCCG
TCGACGCCCTCGGCTTTGGACGCTCAGAAAAGCCGGTCAACGCCCCCTATGGACTGCACTTGCAGAGCGATCTCTACGCC
GGTCTCCTGACCGCACTCGGTATCGAGCGTGCCGTCTTCGTAGCGCATTCGATGGGAGGCAAATACGCGCTGGCGACCGC
CCTCCGCCATCCCCAACGGATCGCCCGCCTGGTACTGATCGCCACCGACGGCTTCATCAAGCCATCGCCACTCACCATCA
TCGGCGGCTGGCCTGTCCTGGGCGAGACCATCCTCTGGCTCTCGGCCCACCCCGCAGTCGTGCGCGCCTTTCTCGCCGCC
GCCTTCCACAACCCCGAACCCTACCTCACCGCCGACCTGATCGAGCGCGGTCGGGCCGCCATCAGCGGTACCGCCAATCG
CCGTGCCCTCACCGCCCTCAGCCGGCGCTACGCCGCCACCGATCTCACCCTCACCGGCCTGCGCGCACGCCTCGGCGAGC
TTCGCGTGCCCACACTGCTCGTCTGGGGCGAACACGACCGCGTCTTCTCGTTCGACTACGCCCGCATTGCCAACCGCGAA
ATCCCCGGCTCGCAGCTCGTCGTCATCCCACACTGCGGCCACTTCCCGCACATCGAAGCCGTGCGCCCGTTTCGTGGCCT
GCTAAATGGCTTCCTGGCCCCGGTACACTCGAACCCATCGTAA

Upstream 100 bases:

>100_bases
GCCGGCAATGAGCAGAAACGCCCTTTTTGCGCTTCATCACTGTCAACCACTCGCCTACCCGTGGTATACTCACTATGGCC
TGTGTCTACGAGGAAAAGCG

Downstream 100 bases:

>100_bases
ACGCCCCACCATCACCGACTGCGCGTCACCCCAATCCGCGGACAGACCTCACGGATCGGGCAGATACTACACCGCGGCGA
CGTAGGGTGACAGATATTCT

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRSRWLRINGYQLHWIEAGQGPAVILLHGFAGSCADWEPTVDWLARQGYRALAVDALGFGRSEKPVNAPYGLHLQSDLYA
GLLTALGIERAVFVAHSMGGKYALATALRHPQRIARLVLIATDGFIKPSPLTIIGGWPVLGETILWLSAHPAVVRAFLAA
AFHNPEPYLTADLIERGRAAISGTANRRALTALSRRYAATDLTLTGLRARLGELRVPTLLVWGEHDRVFSFDYARIANRE
IPGSQLVVIPHCGHFPHIEAVRPFRGLLNGFLAPVHSNPS

Sequences:

>Translated_280_residues
MRSRWLRINGYQLHWIEAGQGPAVILLHGFAGSCADWEPTVDWLARQGYRALAVDALGFGRSEKPVNAPYGLHLQSDLYA
GLLTALGIERAVFVAHSMGGKYALATALRHPQRIARLVLIATDGFIKPSPLTIIGGWPVLGETILWLSAHPAVVRAFLAA
AFHNPEPYLTADLIERGRAAISGTANRRALTALSRRYAATDLTLTGLRARLGELRVPTLLVWGEHDRVFSFDYARIANRE
IPGSQLVVIPHCGHFPHIEAVRPFRGLLNGFLAPVHSNPS
>Mature_280_residues
MRSRWLRINGYQLHWIEAGQGPAVILLHGFAGSCADWEPTVDWLARQGYRALAVDALGFGRSEKPVNAPYGLHLQSDLYA
GLLTALGIERAVFVAHSMGGKYALATALRHPQRIARLVLIATDGFIKPSPLTIIGGWPVLGETILWLSAHPAVVRAFLAA
AFHNPEPYLTADLIERGRAAISGTANRRALTALSRRYAATDLTLTGLRARLGELRVPTLLVWGEHDRVFSFDYARIANRE
IPGSQLVVIPHCGHFPHIEAVRPFRGLLNGFLAPVHSNPS

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=304, Percent_Identity=25.9868421052632, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87081721, Length=271, Percent_Identity=28.4132841328413, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30664; Mature: 30664

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRWLRINGYQLHWIEAGQGPAVILLHGFAGSCADWEPTVDWLARQGYRALAVDALGFG
CCCCEEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCCC
RSEKPVNAPYGLHLQSDLYAGLLTALGIERAVFVAHSMGGKYALATALRHPQRIARLVLI
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCHHHHEEEEEE
ATDGFIKPSPLTIIGGWPVLGETILWLSAHPAVVRAFLAAAFHNPEPYLTADLIERGRAA
EECCCCCCCCEEEECCCHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCEEHHHHHCCCCE
ISGTANRRALTALSRRYAATDLTLTGLRARLGELRVPTLLVWGEHDRVFSFDYARIANRE
ECCCCCHHHHHHHHHHHHHCCEEEHHHHHHHCCCCCCEEEEECCCCCEEEEHHHHHHCCC
IPGSQLVVIPHCGHFPHIEAVRPFRGLLNGFLAPVHSNPS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MRSRWLRINGYQLHWIEAGQGPAVILLHGFAGSCADWEPTVDWLARQGYRALAVDALGFG
CCCCEEEECCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCCC
RSEKPVNAPYGLHLQSDLYAGLLTALGIERAVFVAHSMGGKYALATALRHPQRIARLVLI
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCHHHHEEEEEE
ATDGFIKPSPLTIIGGWPVLGETILWLSAHPAVVRAFLAAAFHNPEPYLTADLIERGRAA
EECCCCCCCCEEEECCCHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCEEHHHHHCCCCE
ISGTANRRALTALSRRYAATDLTLTGLRARLGELRVPTLLVWGEHDRVFSFDYARIANRE
ECCCCCHHHHHHHHHHHHHCCEEEHHHHHHHCCCCCCEEEEECCCCCEEEEHHHHHHCCC
IPGSQLVVIPHCGHFPHIEAVRPFRGLLNGFLAPVHSNPS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA