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The map label for this gene is yugJ [H]

Identifier: 222150835

GI number: 222150835

Start: 620819

End: 621979

Strand: Direct

Name: yugJ [H]

Synonym: MCCL_0585

Alternate gene names: 222150835

Gene position: 620819-621979 (Clockwise)

Preceding gene: 222150834

Following gene: 222150838

Centisome position: 29.53

GC content: 38.16

Gene sequence:

>1161_bases
ATGAATCAATTTACATTTTATAATCCAGTTAAACTGGTATTTGGAAAAGAAAGTCTAGAACAGCTGAACGATATGGTGAA
AGCTTATAGTAACGGAAATAAAGTACTACTTACATATGGAGGAGGAAGCATTAAGAAGACAGGTCTTTATGACAAAGTAA
TGGAAAAGCTATCAGACTTTGAAGTATTTGAACTGTCTGGAATTGAACCGAATCCACGTGTTGAAACGGTACGTAAAGGC
GTAGAAATCGTTAAAGAGAATAATATCGATTTTATCGTAGCAGTCGGTGGTGGATCTGTTATCGATGGTACGAAATTGAT
TGCAGCTGCAAGTAAATATGACGGTGATGCTTGGGATATCGTTACACGTAAAGCACAAGTACAAGATGCTGTACCTTTTG
GAACGGTATTAACATTATCGGCAACTGGTTCTGAAATGAACTCAGGCAGTGTTATTACAAACTGGGAGACAAACGAGAAG
TTAGGTTGGGGTTCACCTCTTGTATTCCCTAAGTTTAGTCTGTTAAATCCGGAAGTTCTATACACATTACCAGAGCATCA
GACAGTGAATGGTATTATCGATAGTATGAGTCACCTATTAGAACAATACTTTAACGAAGCAGATAATACAGAAGTACAAG
ACCAGATGATCAGTGGCGTACTGAAGACTGTGATGAAGACTGGGCCAATTGCAGTGAAAGAACCAGAAAATTACGAAGCA
CGTGAGACATTGATGTTATCATCAACAGTTGCATTAAACGGCTTCTTACGATTAGGCTACGTTGGTGACTGGGCAACACA
TAATCTAGAACATGCAGTAAGTGCTGTATACGATATTGCACACGGTGCAGGTCTTGCGATATTATTCCCTGAGTGGATGC
GTTATGTAAGTAAGCAGTCTCCTGCACGATTTGTGAAGTTTGCTGAAGATGTGTTCAATGTAGAGGCATCTTTGTCAGAT
GAAGAAAAGATTAATTATGCGATTGATGAAATTCAGAACTTCTGGACAAGTTTAGGAGCGAAACAGAAGTTATCTGATTA
TGGAGTGAAAGCTGAAGATTTAGAAGCATTTGCAGATAAAACTTTAATCTATGGTCCATTCGGTAACTTCTATAAGTTAC
AACGTGAAGACATACTAGCAATCTATAAAGCAGCATTATAA

Upstream 100 bases:

>100_bases
CACGTTACCGTGGATGGGCTTAATCATTTTCAATTCTATATTTTTTATGACATAATATGCTTACTTGCATGTTATGTCAT
TTTTATTTCAGGAGGAAGTA

Downstream 100 bases:

>100_bases
TATTCAACAGTAGTAAAAAAGCAGCGGATTCCGCTGCTTTTTTACATGTATTGTTCTGCTTTTGCGATTTGATTCATCAC
GCTTTTTGTACTTGTTCCAT

Product: NADH-dependent butanol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNQFTFYNPVKLVFGKESLEQLNDMVKAYSNGNKVLLTYGGGSIKKTGLYDKVMEKLSDFEVFELSGIEPNPRVETVRKG
VEIVKENNIDFIVAVGGGSVIDGTKLIAAASKYDGDAWDIVTRKAQVQDAVPFGTVLTLSATGSEMNSGSVITNWETNEK
LGWGSPLVFPKFSLLNPEVLYTLPEHQTVNGIIDSMSHLLEQYFNEADNTEVQDQMISGVLKTVMKTGPIAVKEPENYEA
RETLMLSSTVALNGFLRLGYVGDWATHNLEHAVSAVYDIAHGAGLAILFPEWMRYVSKQSPARFVKFAEDVFNVEASLSD
EEKINYAIDEIQNFWTSLGAKQKLSDYGVKAEDLEAFADKTLIYGPFGNFYKLQREDILAIYKAAL

Sequences:

>Translated_386_residues
MNQFTFYNPVKLVFGKESLEQLNDMVKAYSNGNKVLLTYGGGSIKKTGLYDKVMEKLSDFEVFELSGIEPNPRVETVRKG
VEIVKENNIDFIVAVGGGSVIDGTKLIAAASKYDGDAWDIVTRKAQVQDAVPFGTVLTLSATGSEMNSGSVITNWETNEK
LGWGSPLVFPKFSLLNPEVLYTLPEHQTVNGIIDSMSHLLEQYFNEADNTEVQDQMISGVLKTVMKTGPIAVKEPENYEA
RETLMLSSTVALNGFLRLGYVGDWATHNLEHAVSAVYDIAHGAGLAILFPEWMRYVSKQSPARFVKFAEDVFNVEASLSD
EEKINYAIDEIQNFWTSLGAKQKLSDYGVKAEDLEAFADKTLIYGPFGNFYKLQREDILAIYKAAL
>Mature_386_residues
MNQFTFYNPVKLVFGKESLEQLNDMVKAYSNGNKVLLTYGGGSIKKTGLYDKVMEKLSDFEVFELSGIEPNPRVETVRKG
VEIVKENNIDFIVAVGGGSVIDGTKLIAAASKYDGDAWDIVTRKAQVQDAVPFGTVLTLSATGSEMNSGSVITNWETNEK
LGWGSPLVFPKFSLLNPEVLYTLPEHQTVNGIIDSMSHLLEQYFNEADNTEVQDQMISGVLKTVMKTGPIAVKEPENYEA
RETLMLSSTVALNGFLRLGYVGDWATHNLEHAVSAVYDIAHGAGLAILFPEWMRYVSKQSPARFVKFAEDVFNVEASLSD
EEKINYAIDEIQNFWTSLGAKQKLSDYGVKAEDLEAFADKTLIYGPFGNFYKLQREDILAIYKAAL

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1789386, Length=390, Percent_Identity=40, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI48994951, Length=394, Percent_Identity=28.6802030456853, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789163, Length=386, Percent_Identity=25.3886010362694, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082107, Length=315, Percent_Identity=24.4444444444444, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787493, Length=383, Percent_Identity=22.976501305483, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17537053, Length=335, Percent_Identity=24.4776119402985, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321181, Length=384, Percent_Identity=26.3020833333333, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 42814; Mature: 42814

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFTFYNPVKLVFGKESLEQLNDMVKAYSNGNKVLLTYGGGSIKKTGLYDKVMEKLSDF
CCCCCEECCEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
EVFELSGIEPNPRVETVRKGVEIVKENNIDFIVAVGGGSVIDGTKLIAAASKYDGDAWDI
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHEEECCCCCCCHHHH
VTRKAQVQDAVPFGTVLTLSATGSEMNSGSVITNWETNEKLGWGSPLVFPKFSLLNPEVL
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHCCCCEEE
YTLPEHQTVNGIIDSMSHLLEQYFNEADNTEVQDQMISGVLKTVMKTGPIAVKEPENYEA
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
RETLMLSSTVALNGFLRLGYVGDWATHNLEHAVSAVYDIAHGAGLAILFPEWMRYVSKQS
HHHHEEHHHHHHHCEEEEEEECCHHHCCHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCC
PARFVKFAEDVFNVEASLSDEEKINYAIDEIQNFWTSLGAKQKLSDYGVKAEDLEAFADK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCC
TLIYGPFGNFYKLQREDILAIYKAAL
EEEECCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNQFTFYNPVKLVFGKESLEQLNDMVKAYSNGNKVLLTYGGGSIKKTGLYDKVMEKLSDF
CCCCCEECCEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
EVFELSGIEPNPRVETVRKGVEIVKENNIDFIVAVGGGSVIDGTKLIAAASKYDGDAWDI
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHEEECCCCCCCHHHH
VTRKAQVQDAVPFGTVLTLSATGSEMNSGSVITNWETNEKLGWGSPLVFPKFSLLNPEVL
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHCCCCEEE
YTLPEHQTVNGIIDSMSHLLEQYFNEADNTEVQDQMISGVLKTVMKTGPIAVKEPENYEA
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCH
RETLMLSSTVALNGFLRLGYVGDWATHNLEHAVSAVYDIAHGAGLAILFPEWMRYVSKQS
HHHHEEHHHHHHHCEEEEEEECCHHHCCHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCC
PARFVKFAEDVFNVEASLSDEEKINYAIDEIQNFWTSLGAKQKLSDYGVKAEDLEAFADK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCC
TLIYGPFGNFYKLQREDILAIYKAAL
EEEECCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]