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The map label for this gene is gudB [H]

Identifier: 222150834

GI number: 222150834

Start: 619497

End: 620741

Strand: Direct

Name: gudB [H]

Synonym: MCCL_0584

Alternate gene names: 222150834

Gene position: 619497-620741 (Clockwise)

Preceding gene: 222150833

Following gene: 222150835

Centisome position: 29.47

GC content: 39.6

Gene sequence:

>1245_bases
ATGTCAGAAACTACTAACTTAGTGTCATCTACACAGGAAATTATTCATGAAGCTTTAGAAAAATTAGGCTTTGATGAAGG
TATGTATGATTTAGTGAAAGAACCATTACGACTATTAACAGTACGCATTCCAGTGCGTATGGATGATGGTTCGGTTAAAA
CTTTCACGGGTTACCGTGCTCAGCATAATGATGCTGTAGGTCCTACTAAAGGTGGGGTACGTTTCCACCCGGATGTTGAT
GAAGATGAAGTAAAGGCTTTATCAATGTGGATGACATTAAAATGCGGTATCGTTGATCTGCCATACGGTGGAGGTAAAGG
GGGTATCGTGTGTGATCCACGTCAGATGAGCATTCATGAAGTTGAACGTTTATCTCGTGGCTATGTTCGTGCGATTTCTC
AAATCGTAGGTCCAACGAAAGATATTCCAGCACCGGACGTATTTACAAACAGTCAGATTATGGCTTGGATGATGGATGAA
TACAGCATGATGGATGCATTCAATTCTCCAGGATTTATTACAGGAAAACCGATTGTACTTGGAGGATCACAAGGACGTGA
CCGTTCAACTGCATTAGGTGTAGTAATTGCAATAGAAGAAGCAGCAAAACGTCGTGGTAAACACATTAAAGATGCTCGAG
TTGTTATTCAAGGTTTCGGTAATGCAGGAAGTTTCTTAGCGAAGTTCTTATATGATGCTGGCGCTAAAGTTGTCGGAATT
TCAGATGCGTATGGTGCTTTACATGATCCAAATGGCTTAGACATCGATTATTTATTAGATCGTCGTGATAGCTTCGGTAC
AGTAACGAATCTTTTTGAAGATACAATCACTAATAAGGAACTATTCGAATTAGATTGTGACATTCTAGTACCAGCTGCAA
TTTCTAACCAGATTACTGGTGATAACGCACATGATATTAAAGCTGATATCGTAGTTGAAGCTGCAAATGGTCCAACAACA
CCTGAAGCAACTAAAATTTTAACTGAACGTGGCATTCTACTTGTGCCAGATGTACTTGCAAGTGCAGGTGGCGTTACAGT
ATCATACTTTGAATGGGTACAGAATAATCAAGGATACTACTGGACAGAAGAAGAAGTTAATGAGAAGTTAAGGGATAAGC
TTGTAACTGCATTTGATAATGTATATACATTGTCTCAAAACAGAAATATTGATATGCGTCTAGCAGCATACATTATCGGG
ATTAAACGTACAGCAGAAGCTGCACGTTACCGTGGATGGGCTTAA

Upstream 100 bases:

>100_bases
AAAATAGTTGGTAGCGTTTTCAATTTTGGACTTTTAAAAGTTTTTTAATATGTTACAATTAACAATGTAGAATTATAGAA
TAAAAAAAGAGGCGAATAAA

Downstream 100 bases:

>100_bases
TCATTTTCAATTCTATATTTTTTATGACATAATATGCTTACTTGCATGTTATGTCATTTTTATTTCAGGAGGAAGTAATG
AATCAATTTACATTTTATAA

Product: NAD-specific glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSETTNLVSSTQEIIHEALEKLGFDEGMYDLVKEPLRLLTVRIPVRMDDGSVKTFTGYRAQHNDAVGPTKGGVRFHPDVD
EDEVKALSMWMTLKCGIVDLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDE
YSMMDAFNSPGFITGKPIVLGGSQGRDRSTALGVVIAIEEAAKRRGKHIKDARVVIQGFGNAGSFLAKFLYDAGAKVVGI
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEDTITNKELFELDCDILVPAAISNQITGDNAHDIKADIVVEAANGPTT
PEATKILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVNEKLRDKLVTAFDNVYTLSQNRNIDMRLAAYIIG
IKRTAEAARYRGWA

Sequences:

>Translated_414_residues
MSETTNLVSSTQEIIHEALEKLGFDEGMYDLVKEPLRLLTVRIPVRMDDGSVKTFTGYRAQHNDAVGPTKGGVRFHPDVD
EDEVKALSMWMTLKCGIVDLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDE
YSMMDAFNSPGFITGKPIVLGGSQGRDRSTALGVVIAIEEAAKRRGKHIKDARVVIQGFGNAGSFLAKFLYDAGAKVVGI
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEDTITNKELFELDCDILVPAAISNQITGDNAHDIKADIVVEAANGPTT
PEATKILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVNEKLRDKLVTAFDNVYTLSQNRNIDMRLAAYIIG
IKRTAEAARYRGWA
>Mature_413_residues
SETTNLVSSTQEIIHEALEKLGFDEGMYDLVKEPLRLLTVRIPVRMDDGSVKTFTGYRAQHNDAVGPTKGGVRFHPDVDE
DEVKALSMWMTLKCGIVDLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEY
SMMDAFNSPGFITGKPIVLGGSQGRDRSTALGVVIAIEEAAKRRGKHIKDARVVIQGFGNAGSFLAKFLYDAGAKVVGIS
DAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEDTITNKELFELDCDILVPAAISNQITGDNAHDIKADIVVEAANGPTTP
EATKILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVNEKLRDKLVTAFDNVYTLSQNRNIDMRLAAYIIGI
KRTAEAARYRGWA

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=342, Percent_Identity=39.4736842105263, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI4885281, Length=342, Percent_Identity=39.1812865497076, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1788059, Length=362, Percent_Identity=31.767955801105, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17544676, Length=352, Percent_Identity=40.0568181818182, Blast_Score=255, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6319256, Length=411, Percent_Identity=28.7104622871046, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6324951, Length=395, Percent_Identity=27.5949367088608, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24649283, Length=328, Percent_Identity=41.1585365853659, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI45549226, Length=345, Percent_Identity=39.7101449275362, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI45553475, Length=345, Percent_Identity=39.7101449275362, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24649460, Length=280, Percent_Identity=38.5714285714286, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 45522; Mature: 45391

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETTNLVSSTQEIIHEALEKLGFDEGMYDLVKEPLRLLTVRIPVRMDDGSVKTFTGYRA
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEEEECCCCEEEEECCCC
QHNDAVGPTKGGVRFHPDVDEDEVKALSMWMTLKCGIVDLPYGGGKGGIVCDPRQMSIHE
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHEEEECCEEECCCCCCCCCEEECCCCCCHHH
VERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSMMDAFNSPGFITGKPIVL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEECCCCEEE
GGSQGRDRSTALGVVIAIEEAAKRRGKHIKDARVVIQGFGNAGSFLAKFLYDAGAKVVGI
ECCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHEEEEEECCCHHHHHHHHHHHCCCEEEEC
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEDTITNKELFELDCDILVPAAISNQITG
CCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCHHEEECCCEEECHHHCCCCCC
DNAHDIKADIVVEAANGPTTPEATKILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYY
CCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEEHHHHCCCCEE
WTEEEVNEKLRDKLVTAFDNVYTLSQNRNIDMRLAAYIIGIKRTAEAARYRGWA
ECHHHHHHHHHHHHHHHHHCEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SETTNLVSSTQEIIHEALEKLGFDEGMYDLVKEPLRLLTVRIPVRMDDGSVKTFTGYRA
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEEEECCCCEEEEECCCC
QHNDAVGPTKGGVRFHPDVDEDEVKALSMWMTLKCGIVDLPYGGGKGGIVCDPRQMSIHE
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHEEEECCEEECCCCCCCCCEEECCCCCCHHH
VERLSRGYVRAISQIVGPTKDIPAPDVFTNSQIMAWMMDEYSMMDAFNSPGFITGKPIVL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCEECCCCEEE
GGSQGRDRSTALGVVIAIEEAAKRRGKHIKDARVVIQGFGNAGSFLAKFLYDAGAKVVGI
ECCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHEEEEEECCCHHHHHHHHHHHCCCEEEEC
SDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEDTITNKELFELDCDILVPAAISNQITG
CCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCHHEEECCCEEECHHHCCCCCC
DNAHDIKADIVVEAANGPTTPEATKILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYY
CCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEEHHHHCCCCEE
WTEEEVNEKLRDKLVTAFDNVYTLSQNRNIDMRLAAYIIGIKRTAEAARYRGWA
ECHHHHHHHHHHHHHHHHHCEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA