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The map label for this gene is bglIIM [H]

Identifier: 220931356

GI number: 220931356

Start: 557074

End: 558234

Strand: Reverse

Name: bglIIM [H]

Synonym: Hore_05110

Alternate gene names: 220931356

Gene position: 558234-557074 (Counterclockwise)

Preceding gene: 220931357

Following gene: 220931354

Centisome position: 21.65

GC content: 34.45

Gene sequence:

>1161_bases
ATGAATGACAGTAGTACCTGTAATGACAATAATATTAACAATTATAAAATAATCAGGTTAAAACATAAATCTAATTTGCA
CTCTTCAGAAATTAAAGCCATTGACTATATAAATAAATTCTTTAACCAGGATAACTTCTACATTGAGGTAAATCCCGGCT
CCGTAAAGATTTATTTAAAAAAACTCGACATGAAAAAGCTGGGTCGGTTAATTAAAGAAATAAACGATACCAGTCCTTTT
CTTCTCAAAATATACCGTACTATAAAAAATATAAAAAGCTATGTCATTGAAAATAACAAAAGGGTTTATGTAGACTATAA
CAGAGAAAAAAAAGTTAAAAACAGAAAGAAAAAGGAAAGAAACAGGCAAAAATATTTTTATGCCAGAAATCATAATTTTA
ATGTCATTAATCAACCATTACCTGAAAAATATATAAATAAAATTATCTGTGGTGACAGTGAACAAATACTTAAAGAAATA
CCTGATAACAGTATAGATCTCATTCTTACTTCCCCGCCATATAACTTTGGACTTGATTACAAAGATTCACGGGATGGCTA
TTACTGGAAAAGTTATTTTAGTAAGTTGTTTTCCATTTTTAAGGAATGTATCAGAATTCTCAAATATGGCGGCCGGATAA
TCATCAACGTCCAGCCCCTCTTTTCAGATTATATCCCCACCCACCACCTGATCAGCAACTTTTTTATAAAAAATAAGATG
ATCTGGAAGGGAGAAATCCTCTGGGAAAAAAATAACTACAACTGCAAATATACAGCCTGGGGTAGCTGGAAAAGCCCCTC
AAGTCCTTATTTAAAATACACCTGGGAGTTTTTAGAGATCTTTGCAAAGGGTAGTTTAAAGAAAAAAGGAGATAAAAAAA
ATATTGATATTACAGGAGAGGAATTTAAAGAATGGGTTTCGGCCAGGTGGTCGATTGCCCCGGTCCGTAATATGAAAAAA
TACCAGCACCCGGCAGTATTCCCCGAGGAACTGGTTTATAGAGTCCTGAAGTTATTCAGTTATAAGGGTGACGTTATCCT
CGATCCCTTTAACGGAACAGGAACCACTACAGCAGTCGCCCACAGACTTAAAAGGAATTATCTGGGGATTGATATCTCAC
CTGATTACTGTAATACAGCCCGTGGCCGTCTTAATCCATAG

Upstream 100 bases:

>100_bases
AACTTAAACTTATCACATAAAAATGTATTGACTGAGTTCTCAGATGAAATAATACCTGAAGATAAATTAAATGGGAAGGA
AGATAGCGGGGGGAAAAACA

Downstream 100 bases:

>100_bases
CCATCTTTAAACGACCATCCTGAAACCAGTTATAAAACTCAGACAGGATTTGATCTGGCTTCCAGGATAAGGTCAATAAC
TTCACGGACAGCCCCGCGGC

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.BglII; N(4)- cytosine-specific methyltransferase BglII [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNDSSTCNDNNINNYKIIRLKHKSNLHSSEIKAIDYINKFFNQDNFYIEVNPGSVKIYLKKLDMKKLGRLIKEINDTSPF
LLKIYRTIKNIKSYVIENNKRVYVDYNREKKVKNRKKKERNRQKYFYARNHNFNVINQPLPEKYINKIICGDSEQILKEI
PDNSIDLILTSPPYNFGLDYKDSRDGYYWKSYFSKLFSIFKECIRILKYGGRIIINVQPLFSDYIPTHHLISNFFIKNKM
IWKGEILWEKNNYNCKYTAWGSWKSPSSPYLKYTWEFLEIFAKGSLKKKGDKKNIDITGEEFKEWVSARWSIAPVRNMKK
YQHPAVFPEELVYRVLKLFSYKGDVILDPFNGTGTTTAVAHRLKRNYLGIDISPDYCNTARGRLNP

Sequences:

>Translated_386_residues
MNDSSTCNDNNINNYKIIRLKHKSNLHSSEIKAIDYINKFFNQDNFYIEVNPGSVKIYLKKLDMKKLGRLIKEINDTSPF
LLKIYRTIKNIKSYVIENNKRVYVDYNREKKVKNRKKKERNRQKYFYARNHNFNVINQPLPEKYINKIICGDSEQILKEI
PDNSIDLILTSPPYNFGLDYKDSRDGYYWKSYFSKLFSIFKECIRILKYGGRIIINVQPLFSDYIPTHHLISNFFIKNKM
IWKGEILWEKNNYNCKYTAWGSWKSPSSPYLKYTWEFLEIFAKGSLKKKGDKKNIDITGEEFKEWVSARWSIAPVRNMKK
YQHPAVFPEELVYRVLKLFSYKGDVILDPFNGTGTTTAVAHRLKRNYLGIDISPDYCNTARGRLNP
>Mature_386_residues
MNDSSTCNDNNINNYKIIRLKHKSNLHSSEIKAIDYINKFFNQDNFYIEVNPGSVKIYLKKLDMKKLGRLIKEINDTSPF
LLKIYRTIKNIKSYVIENNKRVYVDYNREKKVKNRKKKERNRQKYFYARNHNFNVINQPLPEKYINKIICGDSEQILKEI
PDNSIDLILTSPPYNFGLDYKDSRDGYYWKSYFSKLFSIFKECIRILKYGGRIIINVQPLFSDYIPTHHLISNFFIKNKM
IWKGEILWEKNNYNCKYTAWGSWKSPSSPYLKYTWEFLEIFAKGSLKKKGDKKNIDITGEEFKEWVSARWSIAPVRNMKK
YQHPAVFPEELVYRVLKLFSYKGDVILDPFNGTGTTTAVAHRLKRNYLGIDISPDYCNTARGRLNP

Specific function: This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=260, Percent_Identity=29.6153846153846, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 45796; Mature: 45796

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSSTCNDNNINNYKIIRLKHKSNLHSSEIKAIDYINKFFNQDNFYIEVNPGSVKIYLK
CCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEE
KLDMKKLGRLIKEINDTSPFLLKIYRTIKNIKSYVIENNKRVYVDYNREKKVKNRKKKER
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHH
NRQKYFYARNHNFNVINQPLPEKYINKIICGDSEQILKEIPDNSIDLILTSPPYNFGLDY
HHHHEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCCCCC
KDSRDGYYWKSYFSKLFSIFKECIRILKYGGRIIINVQPLFSDYIPTHHLISNFFIKNKM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHCEE
IWKGEILWEKNNYNCKYTAWGSWKSPSSPYLKYTWEFLEIFAKGSLKKKGDKKNIDITGE
EEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
EFKEWVSARWSIAPVRNMKKYQHPAVFPEELVYRVLKLFSYKGDVILDPFNGTGTTTAVA
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
HRLKRNYLGIDISPDYCNTARGRLNP
HHHHHHCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDSSTCNDNNINNYKIIRLKHKSNLHSSEIKAIDYINKFFNQDNFYIEVNPGSVKIYLK
CCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEE
KLDMKKLGRLIKEINDTSPFLLKIYRTIKNIKSYVIENNKRVYVDYNREKKVKNRKKKER
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHHHHHHHHHHH
NRQKYFYARNHNFNVINQPLPEKYINKIICGDSEQILKEIPDNSIDLILTSPPYNFGLDY
HHHHEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCCCCC
KDSRDGYYWKSYFSKLFSIFKECIRILKYGGRIIINVQPLFSDYIPTHHLISNFFIKNKM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHHCEE
IWKGEILWEKNNYNCKYTAWGSWKSPSSPYLKYTWEFLEIFAKGSLKKKGDKKNIDITGE
EEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
EFKEWVSARWSIAPVRNMKKYQHPAVFPEELVYRVLKLFSYKGDVILDPFNGTGTTTAVA
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
HRLKRNYLGIDISPDYCNTARGRLNP
HHHHHHCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9073062 [H]