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The map label for this gene is astE [H]

Identifier: 218709686

GI number: 218709686

Start: 1822303

End: 1823331

Strand: Reverse

Name: astE [H]

Synonym: VS_1697

Alternate gene names: 218709686

Gene position: 1823331-1822303 (Counterclockwise)

Preceding gene: 218709687

Following gene: 218709685

Centisome position: 55.26

GC content: 45.0

Gene sequence:

>1029_bases
ATGACGAAGACCCTCTTTCGCCAATCATTTCTTTTTGACAGCCTAGATCTTGAGCAAGAAGTGGTTGCAGGACAGACCGT
ACTGAGTAACGGTGTTCAAATTAAGCTTCATCAACGCGGTGTATTGGAAGTGATTCCCGCAGAGTATAATTCTGAGACCA
AGAACATCATCTTTTCAACAGGTGTTCATGGCGACGAAACTTCACCAATGGAGCTGATTGATAAGCTCATTGAGGATATT
GAAACCGGCTTCCAAGTCGTGACAGCAAGATGTTTGTTCATCATTGCTCACCCAGAAGCGACCAATGCGCATACGCGTTT
TCTTGATGTGAATATGAACCGTCTGTTTGATGAAAAACAGTACGAGAGTAATCGAGAAGTGGACATCGCGAAGAACCTTA
AGTTCTTGGTTACTGAGTTTTACAAGGAAACGGTGCCTGCCACTCGTTGGCACTTAGATCTGCACTGTGCGATCCGTTTG
TCTAAGCATTACTCTTTCGCCGTAAGCCCTAAGGTTCGCCACGAAGTTCGCAGTAAAGCCTTGTTCGATTTCATTAACAG
TGCTCACGTTGAAGCGGTGTTGTTATCCAATGCGCCAACGAGCACCTTCAGTTGGTACAGCGCTGAAAACTTTGAAGCCC
AAGCACTGACAATGGAGCTAGGGCAGGTAGCAAGAATTGGTGAGAACCAGCTTGATAAGTTAACGGCTTTCGATTTGGCA
ATGCGCAATTTGATTGCTGAAATAGAACCAGAGCATTTGCCAAAGAAAACCATTACTTATCGCGTAAGCCGAACCATTGT
TCGTTTGCATGATGATTTTGATTTCATGTTCTCTGATGCCGTTGAAAACTTTACGGCGTTCAAGCACGGTGAAGTGTTTG
GTCATGATGGTGATAAGCCATTGATGGCGAAGAATGAAAACGAAGCAGTAGTGTTCCCAAATCGCAACGTTGCTATCGGT
CAACGAGCGGCCTTAATGGTGTGCGAAGTTGAAACACGATTCGACCACGGTCAGCTTGTGTACGATTAA

Upstream 100 bases:

>100_bases
TGTGAGTGATAACGGAGTTTCTATTGATAAGTGCGATCCTAGCAACACAATTTCTATGTACAATTTATTAACATACGGCC
GTGCAATTAAGGAGTAGCGC

Downstream 100 bases:

>100_bases
TACCGACTCGGGTTTAAACGACGAAAACAACCGCTCAACTGGTTGAAATTTCAAGGTTCACATTACGGTGTGAGCCTTGA
GTTTATTTGGGGATACAGGT

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTKTLFRQSFLFDSLDLEQEVVAGQTVLSNGVQIKLHQRGVLEVIPAEYNSETKNIIFSTGVHGDETSPMELIDKLIEDI
ETGFQVVTARCLFIIAHPEATNAHTRFLDVNMNRLFDEKQYESNREVDIAKNLKFLVTEFYKETVPATRWHLDLHCAIRL
SKHYSFAVSPKVRHEVRSKALFDFINSAHVEAVLLSNAPTSTFSWYSAENFEAQALTMELGQVARIGENQLDKLTAFDLA
MRNLIAEIEPEHLPKKTITYRVSRTIVRLHDDFDFMFSDAVENFTAFKHGEVFGHDGDKPLMAKNENEAVVFPNRNVAIG
QRAALMVCEVETRFDHGQLVYD

Sequences:

>Translated_342_residues
MTKTLFRQSFLFDSLDLEQEVVAGQTVLSNGVQIKLHQRGVLEVIPAEYNSETKNIIFSTGVHGDETSPMELIDKLIEDI
ETGFQVVTARCLFIIAHPEATNAHTRFLDVNMNRLFDEKQYESNREVDIAKNLKFLVTEFYKETVPATRWHLDLHCAIRL
SKHYSFAVSPKVRHEVRSKALFDFINSAHVEAVLLSNAPTSTFSWYSAENFEAQALTMELGQVARIGENQLDKLTAFDLA
MRNLIAEIEPEHLPKKTITYRVSRTIVRLHDDFDFMFSDAVENFTAFKHGEVFGHDGDKPLMAKNENEAVVFPNRNVAIG
QRAALMVCEVETRFDHGQLVYD
>Mature_341_residues
TKTLFRQSFLFDSLDLEQEVVAGQTVLSNGVQIKLHQRGVLEVIPAEYNSETKNIIFSTGVHGDETSPMELIDKLIEDIE
TGFQVVTARCLFIIAHPEATNAHTRFLDVNMNRLFDEKQYESNREVDIAKNLKFLVTEFYKETVPATRWHLDLHCAIRLS
KHYSFAVSPKVRHEVRSKALFDFINSAHVEAVLLSNAPTSTFSWYSAENFEAQALTMELGQVARIGENQLDKLTAFDLAM
RNLIAEIEPEHLPKKTITYRVSRTIVRLHDDFDFMFSDAVENFTAFKHGEVFGHDGDKPLMAKNENEAVVFPNRNVAIGQ
RAALMVCEVETRFDHGQLVYD

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate [H]

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788040, Length=321, Percent_Identity=30.8411214953271, Blast_Score=161, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007036
- InterPro:   IPR016681 [H]

Pfam domain/function: PF04952 AstE_AspA [H]

EC number: =3.5.1.96 [H]

Molecular weight: Translated: 39016; Mature: 38884

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLFRQSFLFDSLDLEQEVVAGQTVLSNGVQIKLHQRGVLEVIPAEYNSETKNIIFST
CCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCEEEEEC
GVHGDETSPMELIDKLIEDIETGFQVVTARCLFIIAHPEATNAHTRFLDVNMNRLFDEKQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEECCHHHHHCHHH
YESNREVDIAKNLKFLVTEFYKETVPATRWHLDLHCAIRLSKHYSFAVSPKVRHEVRSKA
HCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCEEEECHHHHHHHHHHH
LFDFINSAHVEAVLLSNAPTSTFSWYSAENFEAQALTMELGQVARIGENQLDKLTAFDLA
HHHHHHCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MRNLIAEIEPEHLPKKTITYRVSRTIVRLHDDFDFMFSDAVENFTAFKHGEVFGHDGDKP
HHHHHHHCCCCCCCCHHEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEECCCCCCC
LMAKNENEAVVFPNRNVAIGQRAALMVCEVETRFDHGQLVYD
CEECCCCCEEEECCCCEECCCCEEEEEEEEECCCCCCCEECC
>Mature Secondary Structure 
TKTLFRQSFLFDSLDLEQEVVAGQTVLSNGVQIKLHQRGVLEVIPAEYNSETKNIIFST
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCEEEEEC
GVHGDETSPMELIDKLIEDIETGFQVVTARCLFIIAHPEATNAHTRFLDVNMNRLFDEKQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEECCHHHHHCHHH
YESNREVDIAKNLKFLVTEFYKETVPATRWHLDLHCAIRLSKHYSFAVSPKVRHEVRSKA
HCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCEEEECHHHHHHHHHHH
LFDFINSAHVEAVLLSNAPTSTFSWYSAENFEAQALTMELGQVARIGENQLDKLTAFDLA
HHHHHHCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MRNLIAEIEPEHLPKKTITYRVSRTIVRLHDDFDFMFSDAVENFTAFKHGEVFGHDGDKP
HHHHHHHCCCCCCCCHHEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEECCCCCCC
LMAKNENEAVVFPNRNVAIGQRAALMVCEVETRFDHGQLVYD
CEECCCCCEEEECCCCEECCCCEEEEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA