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The map label for this gene is btuC [H]

Identifier: 218709685

GI number: 218709685

Start: 1821174

End: 1822169

Strand: Reverse

Name: btuC [H]

Synonym: VS_1696

Alternate gene names: 218709685

Gene position: 1822169-1821174 (Counterclockwise)

Preceding gene: 218709686

Following gene: 218709684

Centisome position: 55.23

GC content: 47.59

Gene sequence:

>996_bases
ATGGATTTCCAACAACTTCTTCACCAAAAACAGCGCAAATGGACCCGAGCCATTTATCTGATGGCAGCACTGCTTGTCGC
GCTGAGTGCGATTTACCTAATGGTTGGCGATCTTTTCATTTCCCCTCTGGGCACCTTGTCCACGTTAGAACAAAAACTAC
TGATTGATTTACGCTTACCTCGACTATTGGCGGCTATTGCTATCGGGGCAGGGTTAGCCGTATCTGGCGCAAGCTTACAA
GTCTTATTGGGTAACGTATTAGCAGAGCCCGGTGTGCTCGGCATTTCTGGCGGCGCAAGCTTGGCGATGGTGATCGTGCT
GTTCTTCTTGCCGTTTGCTCCTACGCCTGAATTATTTATGATTGCCGCCGTTTTAGGCTCACTCTGTTTTACCGTGATTC
TGGTGAGTATGGTTAAAACGATGCGACTGACTACGGCCAAGTTATTGCTGGTGGGTGTTGCGTTAGGCATTCTTTCTGGC
GCGATGGTGACGTGGGCATTCTATTTTAGTGATGATCTTAGCTTACGCTTATTGATGTATTGGCTGATGGGTAGTTTGGG
TGGTGTAACTTGGTACCAGCACTCGCTCACGTTAGTAATGATCCCCGTTATTATCTGGCTGTGCCTGCAAGGTAGCAAGC
TAGACAAACTGATGATTGGCGAGACCCATGCTGCGCAGTTAGGCGTGAACGTTCCGAGATTACGTTGGCGTCTAATCTTC
GCTGTGTCTATTTTAGTCGGCTGCGCAGTAGCGTTAGGTGGCGTGATCAGCTTTGTGGGTTTGGTGGTGCCACACTTACT
CCGTTTAGCGATTGGTACCGACAACCGATACCTTCTTCCTTTGTCTGCCGTTGCAGGTGCCGCGTTACTGGTGTTTGCTG
ATATTTGTGCAAGAACTTTATTAGATTCTGCAGAATTACCCTTGGGTGTGATGACCACCAGTATCGGTGCACCAATCTTC
ATTTGGATGTTAATTAAAAATCATGATTCAAATTAA

Upstream 100 bases:

>100_bases
CTCAACTGGTTGAAATTTCAAGGTTCACATTACGGTGTGAGCCTTGAGTTTATTTGGGGATACAGGTAAGGTTACGCGAA
CAATTTCAAAGTAGCTTGAT

Downstream 100 bases:

>100_bases
GAGCCTGAGCGTCGGCGCTCGTTTATTACCACTATCATTTGAGCTCAAGCAAGGGCAGGTGACTCATGTCATCGGTCCTA
ATGGCAGTGGAAAAAGTACC

Product: vtamin B12-transporter permease

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MDFQQLLHQKQRKWTRAIYLMAALLVALSAIYLMVGDLFISPLGTLSTLEQKLLIDLRLPRLLAAIAIGAGLAVSGASLQ
VLLGNVLAEPGVLGISGGASLAMVIVLFFLPFAPTPELFMIAAVLGSLCFTVILVSMVKTMRLTTAKLLLVGVALGILSG
AMVTWAFYFSDDLSLRLLMYWLMGSLGGVTWYQHSLTLVMIPVIIWLCLQGSKLDKLMIGETHAAQLGVNVPRLRWRLIF
AVSILVGCAVALGGVISFVGLVVPHLLRLAIGTDNRYLLPLSAVAGAALLVFADICARTLLDSAELPLGVMTTSIGAPIF
IWMLIKNHDSN

Sequences:

>Translated_331_residues
MDFQQLLHQKQRKWTRAIYLMAALLVALSAIYLMVGDLFISPLGTLSTLEQKLLIDLRLPRLLAAIAIGAGLAVSGASLQ
VLLGNVLAEPGVLGISGGASLAMVIVLFFLPFAPTPELFMIAAVLGSLCFTVILVSMVKTMRLTTAKLLLVGVALGILSG
AMVTWAFYFSDDLSLRLLMYWLMGSLGGVTWYQHSLTLVMIPVIIWLCLQGSKLDKLMIGETHAAQLGVNVPRLRWRLIF
AVSILVGCAVALGGVISFVGLVVPHLLRLAIGTDNRYLLPLSAVAGAALLVFADICARTLLDSAELPLGVMTTSIGAPIF
IWMLIKNHDSN
>Mature_331_residues
MDFQQLLHQKQRKWTRAIYLMAALLVALSAIYLMVGDLFISPLGTLSTLEQKLLIDLRLPRLLAAIAIGAGLAVSGASLQ
VLLGNVLAEPGVLGISGGASLAMVIVLFFLPFAPTPELFMIAAVLGSLCFTVILVSMVKTMRLTTAKLLLVGVALGILSG
AMVTWAFYFSDDLSLRLLMYWLMGSLGGVTWYQHSLTLVMIPVIIWLCLQGSKLDKLMIGETHAAQLGVNVPRLRWRLIF
AVSILVGCAVALGGVISFVGLVVPHLLRLAIGTDNRYLLPLSAVAGAALLVFADICARTLLDSAELPLGVMTTSIGAPIF
IWMLIKNHDSN

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Involved in the translocation of the substrate across the membrane [H]

COG id: COG4139

COG function: function code H; ABC-type cobalamin transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788004, Length=323, Percent_Identity=43.9628482972136, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1790740, Length=282, Percent_Identity=40.4255319148936, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790741, Length=291, Percent_Identity=35.0515463917526, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786805, Length=290, Percent_Identity=33.448275862069, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786347, Length=280, Percent_Identity=32.8571428571429, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786804, Length=306, Percent_Identity=30.718954248366, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 35538; Mature: 35538

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQQLLHQKQRKWTRAIYLMAALLVALSAIYLMVGDLFISPLGTLSTLEQKLLIDLRLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RLLAAIAIGAGLAVSGASLQVLLGNVLAEPGVLGISGGASLAMVIVLFFLPFAPTPELFM
HHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHH
IAAVLGSLCFTVILVSMVKTMRLTTAKLLLVGVALGILSGAMVTWAFYFSDDLSLRLLMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WLMGSLGGVTWYQHSLTLVMIPVIIWLCLQGSKLDKLMIGETHAAQLGVNVPRLRWRLIF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCHHHHHHHHH
AVSILVGCAVALGGVISFVGLVVPHLLRLAIGTDNRYLLPLSAVAGAALLVFADICARTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
LDSAELPLGVMTTSIGAPIFIWMLIKNHDSN
HCCCCCCHHHHHHHCCCHHHHHEEHHCCCCC
>Mature Secondary Structure
MDFQQLLHQKQRKWTRAIYLMAALLVALSAIYLMVGDLFISPLGTLSTLEQKLLIDLRLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RLLAAIAIGAGLAVSGASLQVLLGNVLAEPGVLGISGGASLAMVIVLFFLPFAPTPELFM
HHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCHHHHH
IAAVLGSLCFTVILVSMVKTMRLTTAKLLLVGVALGILSGAMVTWAFYFSDDLSLRLLMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
WLMGSLGGVTWYQHSLTLVMIPVIIWLCLQGSKLDKLMIGETHAAQLGVNVPRLRWRLIF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCHHHHHHHHH
AVSILVGCAVALGGVISFVGLVVPHLLRLAIGTDNRYLLPLSAVAGAALLVFADICARTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
LDSAELPLGVMTTSIGAPIFIWMLIKNHDSN
HCCCCCCHHHHHHHCCCHHHHHEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]