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The map label for this gene is topA [H]

Identifier: 197119084

GI number: 197119084

Start: 3127358

End: 3129697

Strand: Reverse

Name: topA [H]

Synonym: Gbem_2711

Alternate gene names: 197119084

Gene position: 3129697-3127358 (Counterclockwise)

Preceding gene: 197119085

Following gene: 197119083

Centisome position: 67.81

GC content: 61.54

Gene sequence:

>2340_bases
ATGTCTCAGAATCTCGTCATCGTTGAGTCTCCCGCCAAGGCGAAGACCATTGAAAAATTCCTCGGCCACGACTACAAGGT
CCTGGCCTCGTTCGGTCACGTGCGCGCCCTCCCCAGCAAGCAGGGGTCGGTGGACACGGAGCACGACTTCGAGCCGAAGT
ACGCCGTCCTCCCCGAGAGCAAAAAGCACATCGACGCCATCAAGAAGGAGATGAAGGGGATCTCCTCGCTCCTCCTGGCG
ACAGACCCCGACCGCGAAGGGGAGGCGATCTCCTGGCACCTTTTGGCCGCGCTCGGCCTGGACGGGAAGAAGAAGCTCCC
GTTCGAGATCAAGCGGGTGGTCTTCCACGAGATCACCAAGGACGCCATCGTGCATGCGGTGGAGAATCCCCGCGACATCG
CCCTTGACTTGGTCGACGCCCAGCAGGCCCGCTCCATCCTCGATTACCTGGTCGGCTTCACCCTCTCCCCGTTTCTTTGG
AAGAAGATCCGCTACGGCCTTTCCGCGGGGCGCGTGCAGTCCGTCGCCCTGAGGCTCATCTGCGAGCGCGAGAAGGAGAT
CCAGGCCTTCAAGGAGCAGGAATACTGGACCATCGCCGCGAAGCTTGAGACCGCCAAGAAGCAGGGGCTCACGGCGACGC
TGGTGGAGGCCGAGGGGAAGAAGCTCGGCAAGTTCGACATCCCGGACCAGGAAACCGCCTTCCGGCACTTCGCCAAGCTC
GGGGGAAAGGAATTCCCGACGCAGGAAGGGGACGAGAAGGGAACGCCGCTGGTGGTCGATAAACCCGCGCACCCGGAGTA
CCGGGTCGACAAGGTCACCAAGAGCGAGAGAAAGCGCCAGCCTTCTCCACCTTTCACCACCTCCACCTTGCAGCAGGAGG
CGGCGCGCAAATTGGGCTTCTCCGCCAAGAAGACCATGTCCACGGCGCAAAAACTCTATGAGGGTATCGACGTCGGCGAA
GGGGCCGTCGGTCTCATCACCTACATGCGTACCGACTCCGTCGCCCTTTCCAACGTGGCGCTGGAAGAGGCCAAGTCGGT
GATTACCTCGCTCTACGGCAAGGAATACGCGCTGGAGAAGCCGCGCTTCTTCAAGAACAAGTCCAAAAACGCCCAGGAAG
CGCACGAGGCGGTCCGCCCGACCTACATCGCCAAGACCCCGATCGAGCTGAAGAAGTTCTTAAGCTCCGACCAGTTCAAG
CTCTACGACCTGATCTGGAAGAGGACCGTCGCCTGCCAGATGGCCGAGGCGCTCCTGGACCAGACCTCGGTCGACATCGG
CGCGGGAGAGGGTTTCCGCTTCCGGGTCGCCGGCACCGTGATCCGCTTCGCCGGCTTCATGAAGCTCTACATCGAGGGGG
TCGACGACGAGACGGAGGACAAGGAGAAGGAAGGCCTGCTTCCGCCGCTGGCCGAGGGGGACATCCTCAAGCTGCAGCAG
CTCCTCCCCGAGCGGCACTTCACCCAGCCCCCGCCGCGCTACACCGAGGCGAGCCTGGTGAAGACCCTGGAGGAGTACGG
CATCGGGCGCCCGTCCACCTACGCCTCCATCATGAACACCCTTACCGAGAGGAAGTACGCGCGTCTCGACAAGAAGCGCT
TCTTCCCCGAGGACGTGGGGATGGTGGTCTCCGACCTTTTGACCAACCACTTCACCCAGTACGTCGACTACAACTTCACC
GCGAACCTGGAAGAAGAACTCGACATGGTCTCGCGCGGCGAGAAGCAGTGGCGCCCGCTCCTGCACGAGTTCTGGGGTCC
CTTCATCAACCTTCTGAAGTTGAAGGAAGGTGAGGTGAACAAGTCCGACCTCACCACCGAGGCGACCAACGAGGTCTGCC
CCGAGTGCGGCAAGCCCCTGGTGGTGAAGCTCGGCAAGTTCGGCAAGTTCTACGCCTGCACCGGCTATCCCGAATGCCGT
TACATCCGTCCCTTGGACAAGGAGACGGGCGAGGTGGTCGAACCGGTGGTTTCCGAGGAAATCTGCGACAAGTGCGGTAG
CCACATGCTGATCAAAGACGGGCGTTTCGGCAAGTACTTGGCCTGCTCCGCCTACCCCAACTGCAAGAACATCCAGCCCT
TGGTGAAGCCCAAGGGGACCGGGATCACCTGCGCCGAATGCGGCAAGGGTGAGCTGATCGAGAAGAAGTCCCGTTTCGGC
AAGCTCTTCTACTCCTGCAACCGCTATCCCGAGTGCAAGTTCGCCCTGTGGGATCTCCCGGTGCAGCAGCCCTGCCCCAA
GTGCGGCTTCCCGCTTTTGATCAAGAAGGTCTACAAGCGCGAAGGCGAGTTCCTCAAGTGCCCGAAAGAAGGGTGCGACT
ACAAGAGCAACCAGTCCTAG

Upstream 100 bases:

>100_bases
CTTGGAAATGAAGGGAGCCATAGTCCAACTCCCGGGCAAGCGCTTCGCAGTTGCGTGAGGCGTTGCCCCCAAAGGCGTAA
AATCCATACTAGGTGGACAC

Downstream 100 bases:

>100_bases
AGCGGGCTGGGCAGGTTTATAGGTGCTACCATGATGCGGGGTCTTAGGACCCCGCTTTGGTTTTTAAGGGATATTCCGGG
GAGCCAGAGACGTGTCCCTC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 779; Mature: 778

Protein sequence:

>779_residues
MSQNLVIVESPAKAKTIEKFLGHDYKVLASFGHVRALPSKQGSVDTEHDFEPKYAVLPESKKHIDAIKKEMKGISSLLLA
TDPDREGEAISWHLLAALGLDGKKKLPFEIKRVVFHEITKDAIVHAVENPRDIALDLVDAQQARSILDYLVGFTLSPFLW
KKIRYGLSAGRVQSVALRLICEREKEIQAFKEQEYWTIAAKLETAKKQGLTATLVEAEGKKLGKFDIPDQETAFRHFAKL
GGKEFPTQEGDEKGTPLVVDKPAHPEYRVDKVTKSERKRQPSPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGIDVGE
GAVGLITYMRTDSVALSNVALEEAKSVITSLYGKEYALEKPRFFKNKSKNAQEAHEAVRPTYIAKTPIELKKFLSSDQFK
LYDLIWKRTVACQMAEALLDQTSVDIGAGEGFRFRVAGTVIRFAGFMKLYIEGVDDETEDKEKEGLLPPLAEGDILKLQQ
LLPERHFTQPPPRYTEASLVKTLEEYGIGRPSTYASIMNTLTERKYARLDKKRFFPEDVGMVVSDLLTNHFTQYVDYNFT
ANLEEELDMVSRGEKQWRPLLHEFWGPFINLLKLKEGEVNKSDLTTEATNEVCPECGKPLVVKLGKFGKFYACTGYPECR
YIRPLDKETGEVVEPVVSEEICDKCGSHMLIKDGRFGKYLACSAYPNCKNIQPLVKPKGTGITCAECGKGELIEKKSRFG
KLFYSCNRYPECKFALWDLPVQQPCPKCGFPLLIKKVYKREGEFLKCPKEGCDYKSNQS

Sequences:

>Translated_779_residues
MSQNLVIVESPAKAKTIEKFLGHDYKVLASFGHVRALPSKQGSVDTEHDFEPKYAVLPESKKHIDAIKKEMKGISSLLLA
TDPDREGEAISWHLLAALGLDGKKKLPFEIKRVVFHEITKDAIVHAVENPRDIALDLVDAQQARSILDYLVGFTLSPFLW
KKIRYGLSAGRVQSVALRLICEREKEIQAFKEQEYWTIAAKLETAKKQGLTATLVEAEGKKLGKFDIPDQETAFRHFAKL
GGKEFPTQEGDEKGTPLVVDKPAHPEYRVDKVTKSERKRQPSPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGIDVGE
GAVGLITYMRTDSVALSNVALEEAKSVITSLYGKEYALEKPRFFKNKSKNAQEAHEAVRPTYIAKTPIELKKFLSSDQFK
LYDLIWKRTVACQMAEALLDQTSVDIGAGEGFRFRVAGTVIRFAGFMKLYIEGVDDETEDKEKEGLLPPLAEGDILKLQQ
LLPERHFTQPPPRYTEASLVKTLEEYGIGRPSTYASIMNTLTERKYARLDKKRFFPEDVGMVVSDLLTNHFTQYVDYNFT
ANLEEELDMVSRGEKQWRPLLHEFWGPFINLLKLKEGEVNKSDLTTEATNEVCPECGKPLVVKLGKFGKFYACTGYPECR
YIRPLDKETGEVVEPVVSEEICDKCGSHMLIKDGRFGKYLACSAYPNCKNIQPLVKPKGTGITCAECGKGELIEKKSRFG
KLFYSCNRYPECKFALWDLPVQQPCPKCGFPLLIKKVYKREGEFLKCPKEGCDYKSNQS
>Mature_778_residues
SQNLVIVESPAKAKTIEKFLGHDYKVLASFGHVRALPSKQGSVDTEHDFEPKYAVLPESKKHIDAIKKEMKGISSLLLAT
DPDREGEAISWHLLAALGLDGKKKLPFEIKRVVFHEITKDAIVHAVENPRDIALDLVDAQQARSILDYLVGFTLSPFLWK
KIRYGLSAGRVQSVALRLICEREKEIQAFKEQEYWTIAAKLETAKKQGLTATLVEAEGKKLGKFDIPDQETAFRHFAKLG
GKEFPTQEGDEKGTPLVVDKPAHPEYRVDKVTKSERKRQPSPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGIDVGEG
AVGLITYMRTDSVALSNVALEEAKSVITSLYGKEYALEKPRFFKNKSKNAQEAHEAVRPTYIAKTPIELKKFLSSDQFKL
YDLIWKRTVACQMAEALLDQTSVDIGAGEGFRFRVAGTVIRFAGFMKLYIEGVDDETEDKEKEGLLPPLAEGDILKLQQL
LPERHFTQPPPRYTEASLVKTLEEYGIGRPSTYASIMNTLTERKYARLDKKRFFPEDVGMVVSDLLTNHFTQYVDYNFTA
NLEEELDMVSRGEKQWRPLLHEFWGPFINLLKLKEGEVNKSDLTTEATNEVCPECGKPLVVKLGKFGKFYACTGYPECRY
IRPLDKETGEVVEPVVSEEICDKCGSHMLIKDGRFGKYLACSAYPNCKNIQPLVKPKGTGITCAECGKGELIEKKSRFGK
LFYSCNRYPECKFALWDLPVQQPCPKCGFPLLIKKVYKREGEFLKCPKEGCDYKSNQS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=638, Percent_Identity=25.3918495297806, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4507635, Length=810, Percent_Identity=22.3456790123457, Blast_Score=109, Evalue=1e-23,
Organism=Escherichia coli, GI1787529, Length=789, Percent_Identity=37.5158428390368, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI48994931, Length=156, Percent_Identity=43.5897435897436, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1788061, Length=568, Percent_Identity=23.943661971831, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=559, Percent_Identity=25.9391771019678, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585251, Length=647, Percent_Identity=24.4204018547141, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 87855; Mature: 87724

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNLVIVESPAKAKTIEKFLGHDYKVLASFGHVRALPSKQGSVDTEHDFEPKYAVLPES
CCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCC
KKHIDAIKKEMKGISSLLLATDPDREGEAISWHLLAALGLDGKKKLPFEIKRVVFHEITK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHH
DAIVHAVENPRDIALDLVDAQQARSILDYLVGFTLSPFLWKKIRYGLSAGRVQSVALRLI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHH
CEREKEIQAFKEQEYWTIAAKLETAKKQGLTATLVEAEGKKLGKFDIPDQETAFRHFAKL
HHHHHHHHHHHHCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
GGKEFPTQEGDEKGTPLVVDKPAHPEYRVDKVTKSERKRQPSPPFTTSTLQQEAARKLGF
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
SAKKTMSTAQKLYEGIDVGEGAVGLITYMRTDSVALSNVALEEAKSVITSLYGKEYALEK
HHHHHHHHHHHHHHCCCCCCCCEEHHEEHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
PRFFKNKSKNAQEAHEAVRPTYIAKTPIELKKFLSSDQFKLYDLIWKRTVACQMAEALLD
CHHHHCCCCCHHHHHHHHCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QTSVDIGAGEGFRFRVAGTVIRFAGFMKLYIEGVDDETEDKEKEGLLPPLAEGDILKLQQ
HCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHH
LLPERHFTQPPPRYTEASLVKTLEEYGIGRPSTYASIMNTLTERKYARLDKKRFFPEDVG
HCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MVVSDLLTNHFTQYVDYNFTANLEEELDMVSRGEKQWRPLLHEFWGPFINLLKLKEGEVN
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSDLTTEATNEVCPECGKPLVVKLGKFGKFYACTGYPECRYIRPLDKETGEVVEPVVSEE
HHHCCHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHHHH
ICDKCGSHMLIKDGRFGKYLACSAYPNCKNIQPLVKPKGTGITCAECGKGELIEKKSRFG
HHHHCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHH
KLFYSCNRYPECKFALWDLPVQQPCPKCGFPLLIKKVYKREGEFLKCPKEGCDYKSNQS
HHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCCCCC
>Mature Secondary Structure 
SQNLVIVESPAKAKTIEKFLGHDYKVLASFGHVRALPSKQGSVDTEHDFEPKYAVLPES
CCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCC
KKHIDAIKKEMKGISSLLLATDPDREGEAISWHLLAALGLDGKKKLPFEIKRVVFHEITK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHH
DAIVHAVENPRDIALDLVDAQQARSILDYLVGFTLSPFLWKKIRYGLSAGRVQSVALRLI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHH
CEREKEIQAFKEQEYWTIAAKLETAKKQGLTATLVEAEGKKLGKFDIPDQETAFRHFAKL
HHHHHHHHHHHHCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
GGKEFPTQEGDEKGTPLVVDKPAHPEYRVDKVTKSERKRQPSPPFTTSTLQQEAARKLGF
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
SAKKTMSTAQKLYEGIDVGEGAVGLITYMRTDSVALSNVALEEAKSVITSLYGKEYALEK
HHHHHHHHHHHHHHCCCCCCCCEEHHEEHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
PRFFKNKSKNAQEAHEAVRPTYIAKTPIELKKFLSSDQFKLYDLIWKRTVACQMAEALLD
CHHHHCCCCCHHHHHHHHCCCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
QTSVDIGAGEGFRFRVAGTVIRFAGFMKLYIEGVDDETEDKEKEGLLPPLAEGDILKLQQ
HCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHH
LLPERHFTQPPPRYTEASLVKTLEEYGIGRPSTYASIMNTLTERKYARLDKKRFFPEDVG
HCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MVVSDLLTNHFTQYVDYNFTANLEEELDMVSRGEKQWRPLLHEFWGPFINLLKLKEGEVN
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSDLTTEATNEVCPECGKPLVVKLGKFGKFYACTGYPECRYIRPLDKETGEVVEPVVSEE
HHHCCHHHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHHHH
ICDKCGSHMLIKDGRFGKYLACSAYPNCKNIQPLVKPKGTGITCAECGKGELIEKKSRFG
HHHHCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHH
KLFYSCNRYPECKFALWDLPVQQPCPKCGFPLLIKKVYKREGEFLKCPKEGCDYKSNQS
HHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]