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The map label for this gene is trmFO

Identifier: 197119083

GI number: 197119083

Start: 3125817

End: 3127124

Strand: Reverse

Name: trmFO

Synonym: Gbem_2710

Alternate gene names: 197119083

Gene position: 3127124-3125817 (Counterclockwise)

Preceding gene: 197119084

Following gene: 197119082

Centisome position: 67.76

GC content: 64.6

Gene sequence:

>1308_bases
ATGACGCAGGAAATAACGGTAATAGGCGGAGGTCTCGCCGGTTGCGAGGCCGCCTGGCAGGCGGCCAAGCGCGGCGTGAA
GGTGCGCCTTTTCGAGATGAAGCCAAACTGCTACAGCGAGGCGCACCACCTTCCAGGGCTGAGCGAGCTGGTCTGCTCCA
ACTCGCTGCGCGGGGACTCCCTGGAGAACGCGGTAGGTCTTCTGAAGGAAGAGCTGCGCCGCTTGCAGTCGCTCTTCATG
GAGGGGGCCGAGGCGACCAAGGTCCCCGCAGGGGGGGCGCTCGCCGTCGACCGCGACCTCTTCTCGCAGTACATCACCTC
CCGCATCGAGAGTCACCCGCTCATCGAGGTGGTCCGGGAAGAGGTGACGCGCATCCCGGAAGAGGGGATCGTGGTGCTCG
CCTCCGGCCCCTTGACCTCCGGCCTCCTGGCCGAAGAGATTGGGCGCCTCGCGGGTAGCTACCTTTATTTTTACGATGCC
ATCGCCCCCATCGTCGCGGCCGATTCCATCGACTACGACAAGGCCTTCCGCGCCTCGCGCTACGGCAAAGGGGACGGCGA
CGACTATCTCAACTGCCCCATGGACGAGGAGCAGTACCAGGCCTTCGTGCGCGAGATCGTCGCTGCCGAGAAGGTGGAGC
CGAAGAGCTTCGAGAAGGTGGTCCATTTCGAGGGGTGCATGCCTATCGAGGAGATGGCGAGCCGTGGGCCGGAGACGCTC
CGCTTCGGTCCGATGAAGCCGGTGGGGCTCGTCGATCCCAGGGTGGGGGTGGAGCCGCACGCGGTGATCCAGCTGCGCCA
GGAAAACCGCGAGGCGACCATGTACAACCTGGTCGGCTTCCAGACGAAGCTCACCTGGCCCGAGCAAAAGCGGATCTTCC
GGATGATCCCGGGGCTAGAGAACGCCCAGTTCTTGAGGCTCGGCTCCATGCACAGGAATACCTTCATCAACGCGCCGGAA
CTGCTTATGGCGACTTGCCAGCTGAAGAGCGACCAAAGGATCTTCTTCGCCGGCCAGATCACCGGAGTAGAGGGGTACGT
GGAGTCCGCCTCCAGCGGCTTCGTGGCCGGCGTCAACGCGGCGAGGCTCGCCCAGGGGGAGGGGCTGGTAGTCCCCCCGG
CGGAAACCGCCATCGGCGCGCTGGCGCGGCACATCACCAACACCGAGGCCGGGCACTTCCAGCCCATGAACGTCAACTAC
GGCCTTTTCCCCTCCCTTCCCGGGCGGGTCAAGAAGAAAGAAAAACGCGGGCTTTTGGCGCAGCGGGGCCTGGAAACGTT
GGAGAAGTGGCTCCCGGAGTTGTCCTGA

Upstream 100 bases:

>100_bases
TTGCGAAGGGGGGACAGGGGGGATTTGCCCCAGACGACACACCCTCTCCCAAGAAAAGGGGGAAGGGGGCTTTGCCTAAG
CAGTTTTTTCAGGAGAAACG

Downstream 100 bases:

>100_bases
TCGTTCACCTCGGCAAGCCCTTTCGGGCGGGAGGTAGGCATGCAAGGAGACAAGGAGAAGACACAGCAGGAGATCAGCGT
CATCGGGCGCCTTATCTCAC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTQEITVIGGGLAGCEAAWQAAKRGVKVRLFEMKPNCYSEAHHLPGLSELVCSNSLRGDSLENAVGLLKEELRRLQSLFM
EGAEATKVPAGGALAVDRDLFSQYITSRIESHPLIEVVREEVTRIPEEGIVVLASGPLTSGLLAEEIGRLAGSYLYFYDA
IAPIVAADSIDYDKAFRASRYGKGDGDDYLNCPMDEEQYQAFVREIVAAEKVEPKSFEKVVHFEGCMPIEEMASRGPETL
RFGPMKPVGLVDPRVGVEPHAVIQLRQENREATMYNLVGFQTKLTWPEQKRIFRMIPGLENAQFLRLGSMHRNTFINAPE
LLMATCQLKSDQRIFFAGQITGVEGYVESASSGFVAGVNAARLAQGEGLVVPPAETAIGALARHITNTEAGHFQPMNVNY
GLFPSLPGRVKKKEKRGLLAQRGLETLEKWLPELS

Sequences:

>Translated_435_residues
MTQEITVIGGGLAGCEAAWQAAKRGVKVRLFEMKPNCYSEAHHLPGLSELVCSNSLRGDSLENAVGLLKEELRRLQSLFM
EGAEATKVPAGGALAVDRDLFSQYITSRIESHPLIEVVREEVTRIPEEGIVVLASGPLTSGLLAEEIGRLAGSYLYFYDA
IAPIVAADSIDYDKAFRASRYGKGDGDDYLNCPMDEEQYQAFVREIVAAEKVEPKSFEKVVHFEGCMPIEEMASRGPETL
RFGPMKPVGLVDPRVGVEPHAVIQLRQENREATMYNLVGFQTKLTWPEQKRIFRMIPGLENAQFLRLGSMHRNTFINAPE
LLMATCQLKSDQRIFFAGQITGVEGYVESASSGFVAGVNAARLAQGEGLVVPPAETAIGALARHITNTEAGHFQPMNVNY
GLFPSLPGRVKKKEKRGLLAQRGLETLEKWLPELS
>Mature_434_residues
TQEITVIGGGLAGCEAAWQAAKRGVKVRLFEMKPNCYSEAHHLPGLSELVCSNSLRGDSLENAVGLLKEELRRLQSLFME
GAEATKVPAGGALAVDRDLFSQYITSRIESHPLIEVVREEVTRIPEEGIVVLASGPLTSGLLAEEIGRLAGSYLYFYDAI
APIVAADSIDYDKAFRASRYGKGDGDDYLNCPMDEEQYQAFVREIVAAEKVEPKSFEKVVHFEGCMPIEEMASRGPETLR
FGPMKPVGLVDPRVGVEPHAVIQLRQENREATMYNLVGFQTKLTWPEQKRIFRMIPGLENAQFLRLGSMHRNTFINAPEL
LMATCQLKSDQRIFFAGQITGVEGYVESASSGFVAGVNAARLAQGEGLVVPPAETAIGALARHITNTEAGHFQPMNVNYG
LFPSLPGRVKKKEKRGLLAQRGLETLEKWLPELS

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=104, Percent_Identity=40.3846153846154, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17534255, Length=103, Percent_Identity=40.7766990291262, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_GEOBB (B5EHR5)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139510.1
- ProteinModelPortal:   B5EHR5
- GeneID:   6782708
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2710
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 47840; Mature: 47709

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEITVIGGGLAGCEAAWQAAKRGVKVRLFEMKPNCYSEAHHLPGLSELVCSNSLRGDS
CCCEEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCHHHHHHCCCCCCCH
LENAVGLLKEELRRLQSLFMEGAEATKVPAGGALAVDRDLFSQYITSRIESHPLIEVVRE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHH
EVTRIPEEGIVVLASGPLTSGLLAEEIGRLAGSYLYFYDAIAPIVAADSIDYDKAFRASR
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
YGKGDGDDYLNCPMDEEQYQAFVREIVAAEKVEPKSFEKVVHFEGCMPIEEMASRGPETL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCE
RFGPMKPVGLVDPRVGVEPHAVIQLRQENREATMYNLVGFQTKLTWPEQKRIFRMIPGLE
EECCCCCCCCCCCCCCCCCHHHEEEHHCCCCHHHHHHHCCEEECCCHHHHHHHHHCCCCC
NAQFLRLGSMHRNTFINAPELLMATCQLKSDQRIFFAGQITGVEGYVESASSGFVAGVNA
CCHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCCCCCEECCCH
ARLAQGEGLVVPPAETAIGALARHITNTEAGHFQPMNVNYGLFPSLPGRVKKKEKRGLLA
HHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHH
QRGLETLEKWLPELS
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQEITVIGGGLAGCEAAWQAAKRGVKVRLFEMKPNCYSEAHHLPGLSELVCSNSLRGDS
CCEEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCHHHHHHCCCCCCCH
LENAVGLLKEELRRLQSLFMEGAEATKVPAGGALAVDRDLFSQYITSRIESHPLIEVVRE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHH
EVTRIPEEGIVVLASGPLTSGLLAEEIGRLAGSYLYFYDAIAPIVAADSIDYDKAFRASR
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
YGKGDGDDYLNCPMDEEQYQAFVREIVAAEKVEPKSFEKVVHFEGCMPIEEMASRGPETL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCE
RFGPMKPVGLVDPRVGVEPHAVIQLRQENREATMYNLVGFQTKLTWPEQKRIFRMIPGLE
EECCCCCCCCCCCCCCCCCHHHEEEHHCCCCHHHHHHHCCEEECCCHHHHHHHHHCCCCC
NAQFLRLGSMHRNTFINAPELLMATCQLKSDQRIFFAGQITGVEGYVESASSGFVAGVNA
CCHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCCCCCEECCCH
ARLAQGEGLVVPPAETAIGALARHITNTEAGHFQPMNVNYGLFPSLPGRVKKKEKRGLLA
HHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHH
QRGLETLEKWLPELS
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA