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The map label for this gene is zraS [H]

Identifier: 197116682

GI number: 197116682

Start: 339633

End: 341117

Strand: Direct

Name: zraS [H]

Synonym: Gbem_0282

Alternate gene names: 197116682

Gene position: 339633-341117 (Clockwise)

Preceding gene: 197116681

Following gene: 197116683

Centisome position: 7.36

GC content: 62.22

Gene sequence:

>1485_bases
GTGCTCTCCATCTGTCTGCTCGTCTTCGGTATCGGGACCTTCGCCTTCCTGACGCTTCGGCGCGAGCAGATGCAGCTCGT
CAATTCCGCTCGCGAATCGAGCGAACTCCTCTTGAACACCATCGAACGCTCCCTCTACAACTCCATGAGGATCGGCAACA
CCGAGGACGTGAAGGTGATCCTGGAGATGGTGGGGCAGAGCGAGAACCTGGTGGGGGTGCGCATCTTCCATCCCCACGGG
GTCATCCTGAAGTCCTCCAACCCCAACGAGGTCGGGCGGGTGGTCGACGAGCGCGACTACAAGCTCTTCCTTGACAACAA
GCGCGAAGGCGTCTTCAACCTTGACGGGCACGGCGAGGTGCTGGGGATGGTTAAGCCGATCTACAACGACCGTCAGTGCA
ACCTCTGCCATGGTTCCAAGAGCCGGATCATCGGTCTTTTGAACGTCAATTACTCCCTGACCGAGACACGGAACCGGATC
GTGGAACTGACCAAGCTCTTCGTGATGTCCACCGTCGCCATCATCGGCTTTCTCTCCCTTGCCATCTCGCTGGTCATGCT
GAAGTTCGTCAAGAAGCCGTTAACCCAGCTTGCCAAGAACATGGCGCGCGTGGAGGCGGGGGATCTCTCGGTGCGCATGA
CCCCCGAGGGGAAGGACGAAATCGGGCGCCTGATCGTCTCCTTCGACTCCATGGTGGATCGGCTGGACCGGGCCAAGAAG
GAGCTGGAGAACTTCCATTTCCAGCAGATGGAGCGCGCCGACCGCCTCGCCTCGGTGGGAGAGATGGCCGCCGGCATCGC
CCACGAGATCAAGAACCCGCTTACCGGCATCGCCGCCGCCATCACGGTGCTCAAAGACGACTTCGCCCCGGAAGACGGCC
GCATCCAGATCATCGGCGAGGTGCTGGAGCAGATCTCCCGGCTGGACAAGACGGTGAACGACCTGCTCTTCTTCGGCAAG
CCCACCGTCGCCGAGCCGACCTGCACCGACATCAACTCGGCGCTCAAAAAGATACTGGTCTTCGCCTCGCAGCACCGTGG
CGGCAAGAACATCGAAAAGCGCCTGGAACTGTCCGAAAATCTCCCCACCGTCTATGTGGACCCGAAGCAGATCCAGCAGG
TCTTCCTGAACCTGTTCCTGAACGCGGTCCAGGCGATGCAGGCTGGGGGGACGCTCACCGTGAAGAGCGGGCTGGCCAAG
GTCGAGGGGGTCGACATGGTGCAGGTGCGGGTGACCGACACCGGCCAGGGGATTCCGCCGCAGATCCTGGAGAAGATCTT
CACCCCGTTTTTCACCACCAAGGCGCAGGGGACCGGTCTCGGCCTCGCCATCAGCCACCGCCTCATCGAGCAGCACGGCG
GGAGGCTTTCCGTGGTGAGCCAGGACGGAATGGGAACCACCTTCACCGTGGAACTCCCCGCCTACTGCCCCGGGCAGCCG
GGGTACTCCGGGCCTGACGCGCCCCCCCCGACGGTGTGCCAGTAA

Upstream 100 bases:

>100_bases
GCGCCCCCGTCCAATCCTGGAACCGGGGGCGTTGTGAAAAGATTCACCCACTCAACCGGAGGCACTCCTGATTTTCAAGA
GTCTGACATCGCGGGTCATC

Downstream 100 bases:

>100_bases
AGAGCGCCAGCAGAAACGACAACAACCGGTCCGCCACCGGATCAGTTGCAGCGGGAAATAACCTTAAGGAGCCGGAATGC
GCAAGATAAAGATTATGGTC

Product: HAMP domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MLSICLLVFGIGTFAFLTLRREQMQLVNSARESSELLLNTIERSLYNSMRIGNTEDVKVILEMVGQSENLVGVRIFHPHG
VILKSSNPNEVGRVVDERDYKLFLDNKREGVFNLDGHGEVLGMVKPIYNDRQCNLCHGSKSRIIGLLNVNYSLTETRNRI
VELTKLFVMSTVAIIGFLSLAISLVMLKFVKKPLTQLAKNMARVEAGDLSVRMTPEGKDEIGRLIVSFDSMVDRLDRAKK
ELENFHFQQMERADRLASVGEMAAGIAHEIKNPLTGIAAAITVLKDDFAPEDGRIQIIGEVLEQISRLDKTVNDLLFFGK
PTVAEPTCTDINSALKKILVFASQHRGGKNIEKRLELSENLPTVYVDPKQIQQVFLNLFLNAVQAMQAGGTLTVKSGLAK
VEGVDMVQVRVTDTGQGIPPQILEKIFTPFFTTKAQGTGLGLAISHRLIEQHGGRLSVVSQDGMGTTFTVELPAYCPGQP
GYSGPDAPPPTVCQ

Sequences:

>Translated_494_residues
MLSICLLVFGIGTFAFLTLRREQMQLVNSARESSELLLNTIERSLYNSMRIGNTEDVKVILEMVGQSENLVGVRIFHPHG
VILKSSNPNEVGRVVDERDYKLFLDNKREGVFNLDGHGEVLGMVKPIYNDRQCNLCHGSKSRIIGLLNVNYSLTETRNRI
VELTKLFVMSTVAIIGFLSLAISLVMLKFVKKPLTQLAKNMARVEAGDLSVRMTPEGKDEIGRLIVSFDSMVDRLDRAKK
ELENFHFQQMERADRLASVGEMAAGIAHEIKNPLTGIAAAITVLKDDFAPEDGRIQIIGEVLEQISRLDKTVNDLLFFGK
PTVAEPTCTDINSALKKILVFASQHRGGKNIEKRLELSENLPTVYVDPKQIQQVFLNLFLNAVQAMQAGGTLTVKSGLAK
VEGVDMVQVRVTDTGQGIPPQILEKIFTPFFTTKAQGTGLGLAISHRLIEQHGGRLSVVSQDGMGTTFTVELPAYCPGQP
GYSGPDAPPPTVCQ
>Mature_494_residues
MLSICLLVFGIGTFAFLTLRREQMQLVNSARESSELLLNTIERSLYNSMRIGNTEDVKVILEMVGQSENLVGVRIFHPHG
VILKSSNPNEVGRVVDERDYKLFLDNKREGVFNLDGHGEVLGMVKPIYNDRQCNLCHGSKSRIIGLLNVNYSLTETRNRI
VELTKLFVMSTVAIIGFLSLAISLVMLKFVKKPLTQLAKNMARVEAGDLSVRMTPEGKDEIGRLIVSFDSMVDRLDRAKK
ELENFHFQQMERADRLASVGEMAAGIAHEIKNPLTGIAAAITVLKDDFAPEDGRIQIIGEVLEQISRLDKTVNDLLFFGK
PTVAEPTCTDINSALKKILVFASQHRGGKNIEKRLELSENLPTVYVDPKQIQQVFLNLFLNAVQAMQAGGTLTVKSGLAK
VEGVDMVQVRVTDTGQGIPPQILEKIFTPFFTTKAQGTGLGLAISHRLIEQHGGRLSVVSQDGMGTTFTVELPAYCPGQP
GYSGPDAPPPTVCQ

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=39.0041493775934, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=35.4430379746835, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1790300, Length=233, Percent_Identity=31.3304721030043, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786783, Length=360, Percent_Identity=25.2777777777778, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788393, Length=293, Percent_Identity=26.2798634812287, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081816, Length=350, Percent_Identity=29.4285714285714, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693157, Length=267, Percent_Identity=30.7116104868914, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789149, Length=347, Percent_Identity=24.207492795389, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994928, Length=263, Percent_Identity=28.8973384030418, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786912, Length=281, Percent_Identity=27.7580071174377, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=303, Percent_Identity=24.7524752475248, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=24.3697478991597, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790861, Length=325, Percent_Identity=24, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789808, Length=308, Percent_Identity=24.3506493506494, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082128, Length=223, Percent_Identity=25.5605381165919, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54420; Mature: 54420

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSICLLVFGIGTFAFLTLRREQMQLVNSARESSELLLNTIERSLYNSMRIGNTEDVKVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LEMVGQSENLVGVRIFHPHGVILKSSNPNEVGRVVDERDYKLFLDNKREGVFNLDGHGEV
HHHHCCCCCEEEEEEECCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCCH
LGMVKPIYNDRQCNLCHGSKSRIIGLLNVNYSLTETRNRIVELTKLFVMSTVAIIGFLSL
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AISLVMLKFVKKPLTQLAKNMARVEAGDLSVRMTPEGKDEIGRLIVSFDSMVDRLDRAKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ELENFHFQQMERADRLASVGEMAAGIAHEIKNPLTGIAAAITVLKDDFAPEDGRIQIIGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEHHHH
VLEQISRLDKTVNDLLFFGKPTVAEPTCTDINSALKKILVFASQHRGGKNIEKRLELSEN
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
LPTVYVDPKQIQQVFLNLFLNAVQAMQAGGTLTVKSGLAKVEGVDMVQVRVTDTGQGIPP
CCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCCEEEEEEEECCCCCCCH
QILEKIFTPFFTTKAQGTGLGLAISHRLIEQHGGRLSVVSQDGMGTTFTVELPAYCPGQP
HHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCC
GYSGPDAPPPTVCQ
CCCCCCCCCCCCCC
>Mature Secondary Structure
MLSICLLVFGIGTFAFLTLRREQMQLVNSARESSELLLNTIERSLYNSMRIGNTEDVKVI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LEMVGQSENLVGVRIFHPHGVILKSSNPNEVGRVVDERDYKLFLDNKREGVFNLDGHGEV
HHHHCCCCCEEEEEEECCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCCH
LGMVKPIYNDRQCNLCHGSKSRIIGLLNVNYSLTETRNRIVELTKLFVMSTVAIIGFLSL
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AISLVMLKFVKKPLTQLAKNMARVEAGDLSVRMTPEGKDEIGRLIVSFDSMVDRLDRAKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ELENFHFQQMERADRLASVGEMAAGIAHEIKNPLTGIAAAITVLKDDFAPEDGRIQIIGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEHHHH
VLEQISRLDKTVNDLLFFGKPTVAEPTCTDINSALKKILVFASQHRGGKNIEKRLELSEN
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
LPTVYVDPKQIQQVFLNLFLNAVQAMQAGGTLTVKSGLAKVEGVDMVQVRVTDTGQGIPP
CCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCCEEEEEEEECCCCCCCH
QILEKIFTPFFTTKAQGTGLGLAISHRLIEQHGGRLSVVSQDGMGTTFTVELPAYCPGQP
HHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCC
GYSGPDAPPPTVCQ
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]