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The map label for this gene is folC [H]

Identifier: 189424301

GI number: 189424301

Start: 1283464

End: 1284750

Strand: Reverse

Name: folC [H]

Synonym: Glov_1236

Alternate gene names: 189424301

Gene position: 1284750-1283464 (Counterclockwise)

Preceding gene: 189424302

Following gene: 189424300

Centisome position: 32.79

GC content: 62.63

Gene sequence:

>1287_bases
GTGCCCGCTTCACAGCAGCTTTCCCGGCTTTTTGGCCGGCGACGTTTCAACATCAAGCCGGGACTTGAACGGATCACGGC
CCTGCTGGAGCGCCACGGTCACCCGGAACGTTCCTTTGCCGCCATCCATGTTGTCGGCACCAACGGCAAAGGCTCTACCG
CCGCATTTCTAGCTGCCATCATTGAACAGGCCGGTTACTGCACCGGCCTGTTTACCTCTCCCCACCTTGTCAGCTACACG
GAACGATTTCGGGTCAACGGGGTTGATTGCCCACCAGAGCAACTTGAGGCGCTTATCCCGGACCTGCTTGAACAGGCCGG
CCCGGATGACACGTTCTTTGAGCTGACCACCGCCCTGGCCTGCCACTGGTTTGCCAGGAACAAGGTTCAGCTGGCCGTGC
TGGAAGCCGGCATGGGGGGACGCAGTGACGCCACTGCCGCAGTAGCGGGCATAGCCACCATCATCAGCCCGATTGCCCTT
GACCATTGTCAGTGGCTGGGTAATGACCTCCAGTCCATTGCAGCTGAGAAAGTCGCCATTGCCAGACCGGGCAGTCCGGT
TATCTCTGCAGTACAACCGCCGGACGTGCAGGCGGTGATTGAGGAGTACTGCCGGACAAACAACAACCGGCTCCTGCTGG
CCGGCCGGGACTTCAACGCAACCAGCAACGGAGCTGACACCGGCCTGACCTTCACCGGCAGCTCCGGCAGCCTGAGCGGT
CTGCACCCCAGCTTGAGCGGCAGCTATCAGACCGGTAACGCCGCTCTTGCACTGGCCGCGGCAGAACAACTGGCGACGCT
CGGTTTTCCCATTTCCCAGCAGGCCATGCAGGCAGGTCTGGCCACAGCACGCTGGCCGGGCCGCCTTGAACGGCTCAGAC
TGGCAGATGGTTCCGAACTGCTGCTGGATGGCGCCCACAACCCGGCAGGTGCGCAGGCATTGGCAGCGGCGCTGCAGGAG
CTTGGTAAACGCAGGATCATCCTGTTACTGGGGATGATGGAAGACAAAGACCTGCAAGGCGTATTGCAACCGTTGCTGCA
ACGGGTTCAGCAGGTTATAACGGTCAGCCCCGCGCAGGAACGCGCTCTTCCGGCCACCGAACTGGCTGCCAGGTGCAGGA
CGGCAGGGACTCCGGCCACAGCCGCAGACTCGGTTGCAGCCGGCCTGGAGGCAGCGCGGGCAGCAGCCGGACCCGGTGAC
TTGATCGTAGCTGCCGGCTCACTCTTTCTGGTGGGTGAACTCAAGGCGCTGCTGGCCGGTGTTCCCTGTGAAGCGGTGCG
GGGGTAA

Upstream 100 bases:

>100_bases
AGTACCTGCTTGACCACGGCATGGTTGACATGATTGTCTCACGGCTTGAGATGCGCAGCACCCTGGGCCAGATTCTGGCC
ATGCTCACCAAGCAGTAGGC

Downstream 100 bases:

>100_bases
TGCGTCTCCCTGTAAACGTAAGACGTGCATGTTGTAAGGATCCTATCTTGAAACTACGTACCTGCATTTTTCTACTATTC
CTGCTGATGCCGGCCCTGCT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPASQQLSRLFGRRRFNIKPGLERITALLERHGHPERSFAAIHVVGTNGKGSTAAFLAAIIEQAGYCTGLFTSPHLVSYT
ERFRVNGVDCPPEQLEALIPDLLEQAGPDDTFFELTTALACHWFARNKVQLAVLEAGMGGRSDATAAVAGIATIISPIAL
DHCQWLGNDLQSIAAEKVAIARPGSPVISAVQPPDVQAVIEEYCRTNNNRLLLAGRDFNATSNGADTGLTFTGSSGSLSG
LHPSLSGSYQTGNAALALAAAEQLATLGFPISQQAMQAGLATARWPGRLERLRLADGSELLLDGAHNPAGAQALAAALQE
LGKRRIILLLGMMEDKDLQGVLQPLLQRVQQVITVSPAQERALPATELAARCRTAGTPATAADSVAAGLEAARAAAGPGD
LIVAAGSLFLVGELKALLAGVPCEAVRG

Sequences:

>Translated_428_residues
MPASQQLSRLFGRRRFNIKPGLERITALLERHGHPERSFAAIHVVGTNGKGSTAAFLAAIIEQAGYCTGLFTSPHLVSYT
ERFRVNGVDCPPEQLEALIPDLLEQAGPDDTFFELTTALACHWFARNKVQLAVLEAGMGGRSDATAAVAGIATIISPIAL
DHCQWLGNDLQSIAAEKVAIARPGSPVISAVQPPDVQAVIEEYCRTNNNRLLLAGRDFNATSNGADTGLTFTGSSGSLSG
LHPSLSGSYQTGNAALALAAAEQLATLGFPISQQAMQAGLATARWPGRLERLRLADGSELLLDGAHNPAGAQALAAALQE
LGKRRIILLLGMMEDKDLQGVLQPLLQRVQQVITVSPAQERALPATELAARCRTAGTPATAADSVAAGLEAARAAAGPGD
LIVAAGSLFLVGELKALLAGVPCEAVRG
>Mature_427_residues
PASQQLSRLFGRRRFNIKPGLERITALLERHGHPERSFAAIHVVGTNGKGSTAAFLAAIIEQAGYCTGLFTSPHLVSYTE
RFRVNGVDCPPEQLEALIPDLLEQAGPDDTFFELTTALACHWFARNKVQLAVLEAGMGGRSDATAAVAGIATIISPIALD
HCQWLGNDLQSIAAEKVAIARPGSPVISAVQPPDVQAVIEEYCRTNNNRLLLAGRDFNATSNGADTGLTFTGSSGSLSGL
HPSLSGSYQTGNAALALAAAEQLATLGFPISQQAMQAGLATARWPGRLERLRLADGSELLLDGAHNPAGAQALAAALQEL
GKRRIILLLGMMEDKDLQGVLQPLLQRVQQVITVSPAQERALPATELAARCRTAGTPATAADSVAAGLEAARAAAGPGDL
IVAAGSLFLVGELKALLAGVPCEAVRG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=352, Percent_Identity=31.5340909090909, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI66932984, Length=352, Percent_Identity=31.5340909090909, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1788654, Length=419, Percent_Identity=31.5035799522673, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71984923, Length=335, Percent_Identity=29.8507462686567, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17553148, Length=335, Percent_Identity=29.8507462686567, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17553150, Length=335, Percent_Identity=29.8507462686567, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323760, Length=424, Percent_Identity=31.6037735849057, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324815, Length=371, Percent_Identity=25.0673854447439, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322718, Length=359, Percent_Identity=25.0696378830084, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24641571, Length=291, Percent_Identity=31.6151202749141, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24581568, Length=231, Percent_Identity=28.1385281385281, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44663; Mature: 44532

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASQQLSRLFGRRRFNIKPGLERITALLERHGHPERSFAAIHVVGTNGKGSTAAFLAAI
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHH
IEQAGYCTGLFTSPHLVSYTERFRVNGVDCPPEQLEALIPDLLEQAGPDDTFFELTTALA
HHHCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CHWFARNKVQLAVLEAGMGGRSDATAAVAGIATIISPIALDHCQWLGNDLQSIAAEKVAI
HHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ARPGSPVISAVQPPDVQAVIEEYCRTNNNRLLLAGRDFNATSNGADTGLTFTGSSGSLSG
ECCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCC
LHPSLSGSYQTGNAALALAAAEQLATLGFPISQQAMQAGLATARWPGRLERLRLADGSEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHCCCCCHHHHEECCCCCEE
LLDGAHNPAGAQALAAALQELGKRRIILLLGMMEDKDLQGVLQPLLQRVQQVITVSPAQE
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHH
RALPATELAARCRTAGTPATAADSVAAGLEAARAAAGPGDLIVAAGSLFLVGELKALLAG
HCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHC
VPCEAVRG
CCHHHCCC
>Mature Secondary Structure 
PASQQLSRLFGRRRFNIKPGLERITALLERHGHPERSFAAIHVVGTNGKGSTAAFLAAI
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHH
IEQAGYCTGLFTSPHLVSYTERFRVNGVDCPPEQLEALIPDLLEQAGPDDTFFELTTALA
HHHCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CHWFARNKVQLAVLEAGMGGRSDATAAVAGIATIISPIALDHCQWLGNDLQSIAAEKVAI
HHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ARPGSPVISAVQPPDVQAVIEEYCRTNNNRLLLAGRDFNATSNGADTGLTFTGSSGSLSG
ECCCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCCCC
LHPSLSGSYQTGNAALALAAAEQLATLGFPISQQAMQAGLATARWPGRLERLRLADGSEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHCCCCCHHHHEECCCCCEE
LLDGAHNPAGAQALAAALQELGKRRIILLLGMMEDKDLQGVLQPLLQRVQQVITVSPAQE
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHH
RALPATELAARCRTAGTPATAADSVAAGLEAARAAAGPGDLIVAAGSLFLVGELKALLAG
HCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHC
VPCEAVRG
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]